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Compact characterization of liquid absorption and emission spectra using linear variable filters integrated with a CMOS imaging camera.

Abstract：

:A compact analysis platform for detecting liquid absorption and emission spectra using a set of optical linear variable filters atop a CMOS image sensor is presented. The working spectral range of the analysis platform can be extended without a reduction in spectral resolution by utilizing multiple linear variable filters with different wavelength ranges on the same CMOS sensor. With optical setup reconfiguration, its capability to measure both absorption and fluorescence emission is demonstrated. Quantitative detection of fluorescence emission down to 0.28 nM for quantum dot dispersions and 32 ng/mL for near-infrared dyes has been demonstrated on a single platform over a wide spectral range, as well as an absorption-based water quality test, showing the versatility of the system across liquid solutions for different emission and absorption bands. Comparison with a commercially available portable spectrometer and an optical spectrum analyzer shows our system has an improved signal-to-noise ratio and acceptable spectral resolution for discrimination of emission spectra, and characterization of colored liquid's absorption characteristics generated by common biomolecular assays. This simple, compact, and versatile analysis platform demonstrates a path towards an integrated optical device that can be utilized for a wide variety of applications in point-of-use testing and point-of-care diagnostics.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Wan Y,Carlson JA,Kesler BA,Peng W,Su P,Al-Mulla SA,Lim SJ,Smith AM,Dallesasse JM,Cunningham BT

doi

10.1038/srep29117

subject

Has Abstract

pub_date

2016-07-08 00:00:00

pages

29117

issn

2045-2322

pii

srep29117

journal_volume

6

pub_type

杂志文章

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