Zinc-modified titanium surface enhances osteoblast differentiation of dental pulp stem cells in vitro.

Abstract:

:Zinc is an essential trace element that plays an important role in differentiation of osteoblasts and bone modeling. This in vitro study aimed to evaluate the osteoblast differentiation of human dental pulp stem cells (DPSCs) on zinc-modified titanium (Zn-Ti) that releases zinc ions from its surface. Based on real-time PCR, alkaline phosphatase (ALP) activity and Western blot analysis data, we investigated osteoblast differentiation of DPSCs cultured on Zn-Ti and controls. DPSCs cultured on Zn-Ti exhibited significantly up-regulated gene expression levels of osteoblast-related genes of type I collagen (Col I), bone morphogenetic protein 2 (BMP2), ALP, runt-related transcription factor 2 (Runx2), osteopontin (OPN), and vascular endothelial growth factor A (VEGF A), as compared with controls. We also investigated extracellular matrix (ECM) mineralization by Alizarin Red S (ARS) staining and found that Zn-Ti significantly promoted ECM mineralization when compared with controls. These findings suggest that the combination of Zn-Ti and DPSCs provides a novel approach for bone regeneration therapy.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Yusa K,Yamamoto O,Takano H,Fukuda M,Iino M

doi

10.1038/srep29462

subject

Has Abstract

pub_date

2016-07-08 00:00:00

pages

29462

issn

2045-2322

pii

srep29462

journal_volume

6

pub_type

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