Abstract：
:Patient-matched transcriptomic studies using tumour samples before and after treatment allow inter-patient heterogeneity to be controlled, but tend not to include an untreated comparison. Here, Illumina BeadArray technology was used to measure dynamic changes in gene expression from thirty-seven paired diagnostic core and surgically excised breast cancer biopsies obtained from women receiving no treatment prior to surgery, to determine the impact of sampling method and tumour heterogeneity. Despite a lack of treatment and perhaps surprisingly, consistent changes in gene expression were identified during the diagnosis-surgery interval (48 up, 2 down; Siggenes FDR 0.05) in a manner independent of both subtype and sampling-interval length. Instead, tumour sampling method was seen to directly impact gene expression, with similar effects additionally identified in six published breast cancer datasets. In contrast with previous findings, our data does not support the concept of a significant wounding or immune response following biopsy in the absence of treatment and instead implicates a hypoxic response following the surgical biopsy. Whilst sampling-related gene expression changes are evident in treated samples, they are secondary to those associated with response to treatment. Nonetheless, sampling method remains a potential confounding factor for neoadjuvant study design.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Pearce DA,Arthur LM,Turnbull AK,Renshaw L,Sabine VS,Thomas JS,Bartlett JM,Dixon JM,Sims AHdoi
10.1038/srep29434subject
Has Abstractpub_date
2016-07-07 00:00:00pages
29434issn
2045-2322pii
srep29434journal_volume
6pub_type
杂志文章abstract：:In this study, the biocompatibility and in vitro degradation behaviour of a commercial zinc-based alloy (Zn-5 Al-4 Mg) were evaluated and compared with that of pure zinc for temporary orthopaedic implant applications. Biocompatibility tests were conducted using human alveolar lung epithelial cells (A549), which showed...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-15873-w
更新日期：2017-11-15 00:00:00
abstract：:In recent years methicillin-resistant Staphylococcus aureus has posed a challenge in treating skin and soft tissue infections. Finding new antimicrobial agents has therefore become imperative. We evaluated the in vitro antimicrobial activity of a synthetic peptide, P6, against multidrug resistant clinical strains of S...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-53926-4
更新日期：2019-11-20 00:00:00
abstract：:Lipolysis-stimulated lipoprotein receptor (LSR) is a unique molecule of tricellular contacts of normal and cancer cells. We investigated how the loss of LSR induced cell migration, invasion and proliferation in endometrial cancer cell line Sawano. mRNAs of amphiregulin (AREG) and TEA domain family member 1 (TEAD1) wer...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37049
更新日期：2017-01-10 00:00:00
abstract：:Tropical Pacific stalagmites are commonly affected by dating uncertainties because of their low U concentration and/or elevated initial 230Th content. This poses problems in establishing reliable trends and periodicities for droughts and pluvial episodes in a region vulnerable to climate change. Here we constrain the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81941-x
更新日期：2021-01-26 00:00:00
abstract：:In most species, fertilization induces Ca2+ transients in the egg. In mammals, the Ca2+ rises are triggered by phospholipase Cζ (PLCζ) released from the sperm; IP3 generated by PLCζ induces Ca2+ release from the intracellular Ca2+ store through IP3 receptor, termed IP3-induced Ca2+ release. Here, we developed new fluo...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40931-w
更新日期：2019-03-18 00:00:00
abstract：:Low miR-96-5p expression is characteristic of many cancers but its role in breast cancer (BCa) remains poorly defined. Here, the role of miR-96-5p in BC development was assessed. We demonstrate that exogenously expressing miR-96-5p inhibits the proliferative, migratory and invasive capacity of BCa cells. Mechanistical...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56571-z
更新日期：2020-01-08 00:00:00
abstract：:Quercetin is a natural flavonoid, which has been shown to have anti hepatitis C virus (HCV) properties. However, the exact mechanisms whereby quercetin impacts the HCV life cycle are not fully understood. We assessed the effect of quercetin on different steps of the HCV life cycle in Huh-7.5 cells and primary human he...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31777
更新日期：2016-08-22 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-47978-9
更新日期：2019-08-09 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2015-06-05 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2016-11-18 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-01-31 00:00:00
abstract：:The gene PFKFB3 encodes for inducible 6-phosphofructo-2-kinase, a glycolysis-regulatory enzyme that protects against diet-induced intestine inflammation. However, it is unclear how nutrient overload regulates PFKFB3 expression and inflammatory responses in intestinal epithelial cells (IECs). In the present study, prim...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28963
更新日期：2016-07-08 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35220-x
更新日期：2018-11-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56258-5
更新日期：2019-12-27 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep20299
更新日期：2016-02-02 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72521-6
更新日期：2020-09-23 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep29831
更新日期：2016-07-20 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep25251
更新日期：2016-05-04 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40546-1
更新日期：2019-03-11 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51224-7
更新日期：2019-10-10 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30260
更新日期：2016-07-25 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-11-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50494-5
更新日期：2019-10-01 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2020-10-19 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39695-0
更新日期：2019-03-05 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04114
更新日期：2014-02-18 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31477
更新日期：2016-08-11 00:00:00
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pub_type： 杂志文章
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更新日期：2020-06-10 00:00:00
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journal_title：Scientific reports
pub_type： 杂志文章
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更新日期：2019-08-21 00:00:00
abstract：:Fatal Familial Insomnia (FFI) is a genetic prion disease caused by a point mutation in the prion protein gene (PRNP) characterized by prominent thalamic atrophy, diffuse astrogliosis and moderate deposition of PrPSc in the brain. Here, for the first time, we demonstrate that the olfactory mucosa (OM) of patients with ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep46269
更新日期：2017-04-07 00:00:00
