Abstract：
:Arrestins desensitize G protein-coupled receptors (GPCRs) and act as mediators of signalling. Here we investigated the interactions of arrestin-1 with two functionally distinct forms of the dim-light photoreceptor rhodopsin. Using unbiased scanning mutagenesis we probed the individual contribution of each arrestin residue to the interaction with the phosphorylated apo-receptor (Ops-P) and the agonist-bound form (Meta II-P). Disruption of the polar core or displacement of the C-tail strengthened binding to both receptor forms. In contrast, mutations of phosphate-binding residues (phosphosensors) suggest the phosphorylated receptor C-terminus binds arrestin differently for Meta II-P and Ops-P. Likewise, mutations within the inter-domain interface, variations in the receptor-binding loops and the C-edge of arrestin reveal different binding modes. In summary, our results indicate that arrestin-1 binding to Meta II-P and Ops-P is similarly dependent on arrestin activation, although the complexes formed with these two receptor forms are structurally distinct.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Peterhans C,Lally CC,Ostermaier MK,Sommer ME,Standfuss Jdoi
10.1038/srep28686subject
Has Abstractpub_date
2016-06-28 00:00:00pages
28686issn
2045-2322pii
srep28686journal_volume
6pub_type
杂志文章abstract：:To date, the significant anti-cancer capacity of cold atmospheric plasma (CAP) on dozens of cancer cell lines has been demonstrated in vitro and in mice models. Conventionally, CAP was directly applied to irradiate cancer cells or tumor tissue. Over past three years, the CAP irradiated media was also found to kill can...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep18339
更新日期：2015-12-17 00:00:00
abstract：:Cholecystokinin (CCK) and leptin are satiety-controlling peptides, yet their interactive roles remain unclear. Here, we addressed this issue using in vitro and in vivo models. In rat C6 glioma cells, leptin pre-treatment enhanced Ca2+ mobilization by a CCK agonist (CCK-8s). This leptin action was reduced by Janus kina...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69035-6
更新日期：2020-07-20 00:00:00
abstract：:Little is known on the causes and pathogenesis of the adipose tissue disorder (familial) Multiple Symmetric Lipomatosis (MSL). In a four-generation MSL-family, we performed whole exome sequencing (WES) in 3 affected individuals and 1 obligate carrier and identified Calcyphosine-like (CAPSL) as the most promising candi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-44382-1
更新日期：2019-06-11 00:00:00
abstract：:Given new distribution patterns of the endangered North Atlantic right whale (NARW; Eubalaena glacialis) population in recent years, an improved understanding of spatio-temporal movements are imperative for the conservation of this species. While so far visual data have provided most information on NARW movements, pas...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13359-3
更新日期：2017-10-18 00:00:00
abstract：:Chronic inflammatory insults compromise immune cell responses and ultimately contribute to pathologic outcomes. Clinically, it has been suggested that bone debris and implant particles, such as polymethylmethacrylate (PMMA), which are persistently released following implant surgery evoke heightened immune, inflammator...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46934-x
更新日期：2019-07-18 00:00:00
abstract：:The Fenton-like activity of nanoceria has attracted intensive attention for wastewater treatment in recent years. During the Fenton-like reaction, the adsorption of organic pollutants on catalyst surface plays a key role in their degradation. In this work, the adsorption-degradation of methylene blue (MB) and Congo re...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36794-2
更新日期：2019-03-21 00:00:00
abstract：:The prevalence of type 2 diabetes (T2D) has increased dramatically in Middle Eastern populations that represent the largest non-European immigrant group in Sweden today. As proneurotensin predicts T2D, the aim of this study was to investigate differences in proneurotensin levels across populations of Middle Eastern an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50040-3
更新日期：2019-09-20 00:00:00
abstract：:High-throughput technologies have identified significant changes in patterns of mRNA expression over cancer development but the functional significance of these changes often rests upon the assumption that observed changes in levels of mRNA accurately reflect changes in levels of their encoded proteins. We systematica...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08502-z
更新日期：2017-08-15 00:00:00
abstract：:Human cardiac stem/progenitor cells (hCPCs) may serve in regenerative medicine to repair the infarcted heart. However, this approach is severely limited by the poor survival of donor cells. Recent studies suggest that the mammalian globin cytoglobin (CYGB) regulates nitric oxide (NO) metabolism and cell death. In the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-11342-6
更新日期：2017-09-07 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-62904-0
更新日期：2020-04-10 00:00:00
abstract：:FTY-720 (Fingolimod) was one of the first compounds authorized for the treatment of multiple sclerosis. Among its other activities, this sphingosine analogue enhances exocytosis in neuroendocrine chromaffin cells, altering the quantal release of catecholamines. Surprisingly, the size of chromaffin granules is reduced ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-55106-w
更新日期：2019-12-05 00:00:00
abstract：:Clinical data have been equivocal and controversial as to the benefits to the brain and cognition of hormone therapy (HT) in postmenopausal women. Recent reevaluation of the role of estrogens proposed that HT may effectively prevent the deleterious effects of aging on cognition, and reduces the risks of dementia, incl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44917
更新日期：2017-03-21 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-65506-y
更新日期：2020-05-15 00:00:00
abstract：:Saliva has immense potential as a diagnostic fluid for identification and monitoring of several systemic diseases. Composition of the microbiome and inflammation has been associated and reflective of oral and overall health. In addition, the relative ease of collection of saliva further strengthens large-scale diagnos...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81420-3
更新日期：2021-01-29 00:00:00
abstract：:Leptin has a central role in the maintenance of energy homeostasis, and its sensitivity is influenced by both the photoperiod and dietary polyphenols. The aim of this study was to investigate the effect of seasonal consumption of polyphenol-rich fruits on the hypothalamic leptin signaling system in non-obese and obese...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31855-y
更新日期：2018-09-11 00:00:00
abstract：:Nitric oxide (NO) regulates plant growth and development as well as responses to stress that enhanced its endogenous production. Arabidopsis plants exposed to a pulse of exogenous NO gas were used for untargeted global metabolomic analyses thus allowing the identification of metabolic processes affected by NO. At earl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep37945
更新日期：2016-11-25 00:00:00
abstract：:KIBRA has been suggested as a key regulator of the hippo pathway, regulating organ size, cell contact inhibition as well as tissue regeneration and tumorigenesis. Recently, alterations of KIBRA expression caused by promotor methylation have been reported for several types of cancer. Our current study aimed to design a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22600-6
更新日期：2018-03-09 00:00:00
abstract：:Keyboards and smartphones allow users to express their thoughts freely via manual control. Hands-free communication can be realized with brain-computer interfaces (BCIs) based on code-modulated visual evoked potentials (c-VEPs). Various variations of such spellers have been developed: Low-target systems, multi-target ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-74143-4
更新日期：2020-10-13 00:00:00
abstract：:Dengue is a mild flu-like arboviral illness caused by dengue virus (DENV) that occurs in tropical and subtropical countries. An increasing number of reports have been indicating that dengue is also associated to neurological manifestations, however, little is known regarding the neuropathogenesis of the disease. Here,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-28137-y
更新日期：2018-06-27 00:00:00
abstract：:The Dirac materials, such as graphene and three-dimensional topological insulators, have attracted much attention because they exhibit novel quantum phenomena with their low energy electrons governed by the relativistic Dirac equations. One particular interest is to generate Dirac cone anisotropy so that the electrons...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05385
更新日期：2014-06-20 00:00:00
abstract：:Autism is a common and often severe neurodevelopmental disorder for which diverse pathophysiological processes have been proposed. Recent gene expression data comparing autistic and control brains suggest that the normal differential gene expression between frontal and temporal cortex is attenuated in autistic brains....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01831
更新日期：2013-01-01 00:00:00
abstract：:Transport of tissue-derived lymphatic fluid and clearance by draining lymph nodes are pivotal for maintenance of fluid homeostasis in the body and for immune-surveillance of the self- and non-self-proteomes. Yet a quantitative analysis of nodal filtration of the tissue-derived proteome present in lymphatic fluid has n...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29614-0
更新日期：2018-07-26 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-55968-0
更新日期：2019-12-27 00:00:00
abstract：:Plasmodium vivax, a major agent of malaria in both temperate and tropical climates, has been thought to be unable to infect humans lacking the Duffy (Fy) blood group antigen because this receptor is critical for erythrocyte invasion. Recent surveys in various endemic regions, however, have reported P. vivax infections...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep35581
更新日期：2016-10-19 00:00:00
abstract：:Bats of the Rhinolophidae and Hipposideridae families, and Pteronotus parnellii, compensate for Doppler shifts generated by their own flight movement. They adjust their call frequency such that the frequency of echoes coming from ahead fall in a specialized frequency range of the hearing system, the auditory fovea, to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22880-y
更新日期：2018-03-15 00:00:00
abstract：:In arid and semiarid regions, low precipitation rates lead to soil salinity problems, which may limit plant establishment, growth, and survival. Herein, we investigated the NaCl stress effect on chlorophyll fluorescence, photosynthetic-pigments, movement and chloroplasts ultrastructure in chlorenchyma cells of Opuntia...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08744-x
更新日期：2017-08-17 00:00:00
abstract：:Faithful DNA replication is a prerequisite for cell proliferation. Several cytological studies have shown that chromosome structures alter in the S-phase of the cell cycle. However, the molecular mechanisms behind the alteration of chromosome structures associated with DNA replication have not been elucidated. Here, w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12720
更新日期：2015-07-30 00:00:00
abstract：:The behavior of clogging has a close relationship with the biofilm attached on inner surface of the pipeline in a drip irrigation system using reclaimed water. Therefore, inhibiting biofilm growth is the key to completely addressing the clogging problem. Water shear forces play a vital role in the formation, developme...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63898-5
更新日期：2020-04-24 00:00:00
abstract：:When one liquid is introduced into another immiscible one, it ultimately fragments due to hydrodynamic instability. In contrast to neck pinch-off without external actuation, the viscous two-fluid system subjected to an oscillatory flow demonstrates higher efficiency in breaking fluid threads. However, the underlying d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31436
更新日期：2016-08-11 00:00:00
abstract：:Obesity causes low-grade inflammation that is involved in male infertility. Interleukin 1 beta (IL1β) plays an important role in this process. A high-fat diet (HFD) is the most common cause of obesity. However, the effect of a HFD on IL1β and its consequence in reproduction remain unclear. We established a HFD model i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00505-0
更新日期：2017-04-03 00:00:00
