Abstract：
:It is known that ribosomal RNA (rRNA) synthesis is regulated by cellular energy and proliferation status. In this study, we investigated rRNA gene transcription in response to cytoskeletal stress. Our data revealed that the cell shape constrained by isotropic but not elongated micropatterns in HeLa cells led to a significant reduction in rRNA transcription dependent on ROCK. Expression of a dominant-active form of ROCK also repressed rRNA transcription. Isotropic constraint and ROCK over-activation led to different types of aberrant F-actin organization, but their suppression effects on rRNA transcription were similarly reversed by inhibition of histone deacetylase (HDAC) or overexpression of a dominant negative form of Nesprin, which shields the signal transmitted from actin filament to the nuclear interior. We further showed that the binding of HDAC1 to the active fraction of rDNA genes is increased by ROCK over-activation, thus reducing H3K9/14 acetylation and suppressing transcription. Our results demonstrate an epigenetic control of active rDNA genes that represses rRNA transcription in response to the cytoskeletal stress.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wu TH,Kuo YY,Lee HH,Kuo JC,Ou MH,Chang ZFdoi
10.1038/srep28685subject
Has Abstractpub_date
2016-06-28 00:00:00pages
28685issn
2045-2322pii
srep28685journal_volume
6pub_type
杂志文章abstract：:Studies suggest that chromium deficiency is associated with elevated levels of fasting blood glucose, circulating insulin, cholesterol and triglycerides, and decreased proportion of lean body mass. However, data directly relating chromium levels to metabolic syndrome (MetS) risk are lacking. A total of 3,648 American ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15606
更新日期：2015-10-22 00:00:00
abstract：:The endoplasmic reticulum (ER) is the main site of protein synthesis in eukaryotic cells and requires a high concentration of luminal chaperones to function. During protein synthesis, ER luminal chaperones are swept along the secretory pathway and must be retrieved to maintain cell viability. ER protein retrieval is a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-73906-3
更新日期：2020-10-09 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-21306-z
更新日期：2018-03-06 00:00:00
abstract：:The aim of this study was to identify novel plasma metabolic signatures with possible relevance during multiple myeloma (MM) development and progression. A biochemical quantitative phenotyping platform based on targeted electrospray ionization tandem mass spectrometry technology was used to aid in the identification o...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75862-4
更新日期：2020-12-14 00:00:00
abstract：:The prion protein (PrPC), a mainly neuronal protein, is known to modulate glucose homeostasis in mouse models. We explored the underlying mechanism in mouse models and the human pancreatic β-cell line 1.1B4. We report expression of PrPC on mouse pancreatic β-cells, where it promoted uptake of iron through divalent-met...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-24786-1
更新日期：2018-04-26 00:00:00
abstract：:Epigenetic silencing through promoter hypermethylation is an important hallmark for the inactivation of tumor-related genes in carcinogenesis. Here we identified the ATP-binding cassette sub-family B member 4 (ABCB4) as a novel epigenetically silenced target gene. We investigated the epigenetic regulation of ABCB4 in ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06899
更新日期：2014-11-04 00:00:00
abstract：:The migration of oligodendrocyte progenitor cells (OPCs) to the white matter is an indispensable requirement for an intact brain function. The mechanism of cell migration in general is not yet completely understood. Nevertheless, evidence is accumulating that besides the coordinated rearrangement of the cytoskeleton, ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01806
更新日期：2013-01-01 00:00:00
abstract：:Metals can transmit light by tunnelling when they possess skin-depth thickness. Tunnelling can be resonantly enhanced if resonators are added to each side of a metal film, such as additional dielectric layers or periodic structures on a metal surface. Here we show that, even with no additional resonators, tunnelling r...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep02423
更新日期：2013-01-01 00:00:00
abstract：:Pathologic diagnoses mainly depend on visual scoring by pathologists, a process that can be time-consuming, laborious, and susceptible to inter- and/or intra-observer variations. This study proposes a novel method to enhance pathologic scoring of renal allograft rejection. A fully automated system using a convolutiona...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41479-5
更新日期：2019-03-26 00:00:00
abstract：:This paper presents the results of the experiments which were performed using the optical biopsy system specially developed for in vivo tissue classification during the percutaneous needle biopsy (PNB) of the liver. The proposed system includes an optical probe of small diameter acceptable for use in the PNB of the li...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71089-5
更新日期：2020-08-26 00:00:00
abstract：:Peatland ecosystem services include drinking water provision, flood mitigation, habitat provision and carbon sequestration. Dissolved organic carbon (DOC) removal is a key treatment process for the supply of potable water downstream from peat-dominated catchments. A transition from peat-forming Sphagnum moss to vascul...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36751
更新日期：2016-11-18 00:00:00
abstract：:Polarimetric imaging has proved its value in medical diagnostics, bionics, remote sensing, astronomy, and in many other wide fields. Pixel-level solid monolithically integrated polarimetric imaging photo-detectors are the trend for infrared polarimetric imaging devices. For better polarimetric imaging performance the ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep06332
更新日期：2014-09-11 00:00:00
abstract：:Cognitive dysfunction in fibromyalgia has been reported, especially memory. Anodal transcranial direct current stimulation (tDCS) over the dorsolateral prefrontal cortex (DLPFC) has been effective in enhancing this function. We tested the effects of eight sessions of tDCS and cognitive training on immediate and delaye...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-018-30127-z
更新日期：2018-08-20 00:00:00
abstract：:Entamoeba possesses a highly divergent mitochondrion-related organelle known as the mitosome. Here, we report the discovery of a novel protein in Entamoeba, which we name Mitosomal β-barrel Outer Membrane Protein of 30 kDa (MBOMP30). Initially identified through in silico analysis, we experimentally confirmed that MBO...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08545
更新日期：2015-02-25 00:00:00
abstract：:Many cardiac pathologies involve changes in tissue structure. Conventional analysis of structural features is extremely time-consuming and subject to observer bias. The possibility to determine spatial interrelations between these features is often not fully exploited. We developed a staining protocol and an ImageJ-ba...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-76932-3
更新日期：2020-11-18 00:00:00
abstract：:Glacial refugia are centers of high biodiversity. Therefore, knowledge on their locations and reactions of associated populations and landscapes to climatic changes is crucial for conservation management. We here investigated the biogeography of a butterfly species linked to open forest habitats. Using mitochondrial a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-79376-x
更新日期：2021-01-08 00:00:00
abstract：:Changes in climate and atmospheric CO2 and nitrogen (N) over the last several decades have induced significant effects on forest carbon (C) cycling. However, contributions of individual factors are largely unknown because of the lack of long observational data and the undifferentiating between intrinsic factors and ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04461
更新日期：2014-03-25 00:00:00
abstract：:Lipoprotein-associated phospholipase A2 (Lp-PLA2) levels are associated with the development of atherosclerosis. We aimed to assess the genetic determinants of Lp-PLA2 activity and mass by genotyping multiple polymorphisms in PLA2G7, the gene encoding Lp-PLA2, among 1258 participants from the Chinese Multi-provincial ...
journal_title：Scientific reports
pub_type： 杂志文章,多中心研究
doi：10.1038/srep37465
更新日期：2016-12-01 00:00:00
abstract：:Ecosystem carbon carrying capacity (CCC) is determined by prevailing climate and natural disturbance regimes, conditions that are projected to change significantly. The interaction of changing climate and its effects on disturbance regimes is expected to affect forest regeneration and growth, which may diminish forest...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02686-0
更新日期：2017-05-25 00:00:00
abstract：:Perhaps, oxidative stress progresses pupation in some Lepidopteran insects; however, the reasons for this remain obscure. In our previous study, we clarified Bombyx mori SOD1 (BmSOD1) and B. mori SOD2 (BmSOD2) proteins respond in common to ultraviolet irradiation (UV) oxidative stress and metamorphosis. This result st...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51163-3
更新日期：2019-10-11 00:00:00
abstract：:Recently proposed tumor fitness measures, based on profiling neoepitopes for reactive viral epitope similarity, have been proposed to predict response to immune checkpoint inhibitors in melanoma and small-cell lung cancer. Here we applied these checkpoint based fitness measures to the matched checkpoint treatment naiv...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61992-2
更新日期：2020-03-19 00:00:00
abstract：:Incomplete or partial observations of network structures pose a serious challenge to theoretical and engineering studies of real networks. To remedy the missing links in real datasets, topology-based link prediction is introduced into the studies of various networks. Due to the complexity of network structures, the ac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-31254-3
更新日期：2018-09-06 00:00:00
abstract：:Epidemiology of acute kidney injury (AKI) in developing countries is under-studied. We evaluated the risk and prognosis of AKI in patients admitted to intensive care units (ICUs) in Egypt. We recruited consecutive adults admitted to ICUs in Alexandria Teaching Hospitals over six months. We used the KDIGO criteria for ...
journal_title：Scientific reports
pub_type： 杂志文章,多中心研究
doi：10.1038/s41598-017-17264-7
更新日期：2017-12-07 00:00:00
abstract：:Plastic represents 60-80% of litter in the ocean. Degradation of plastic to small fragments leads to the formation of microplastics (MPs <5 mm) and nanoplastics (NPs <1 µm). One of the most widely used and representative plastics found in the ocean is polystyrene (PS). Among marine organisms, the immune system of biva...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65596-8
更新日期：2020-05-25 00:00:00
abstract：:In this study, we describe a web-based resource, developed for assisting the scientific community in designing an effective therapeutics against the Ebola virus. Firstly, we predicted and identified experimentally validated epitopes in each of the antigens/proteins of the five known ebolaviruses. Secondly, we generate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24782
更新日期：2016-04-26 00:00:00
abstract：:This article shows that nanodiamonds can transmigrate through the insect cuticle easily, and the doses used were not hemocytotoxic and did not cause inhibition of cellular and humoral immune responses in larvae, pupae and adults of Tenebrio molitor. The examination of the nanodiamond biodistribution in insect cells de...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-46720-9
更新日期：2019-07-17 00:00:00
abstract：:Geographically diverse samples from strawberry exhibiting symptoms of Strawberry Green Petal (SbGP), periwinkle plants with virescence, and blackberry, blueberry, and raspberry plants displaying yellowing and inedible fruits, were assayed for the presence of phytoplasma DNA. PCR targeting the 16S rRNA-encoding gene an...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00895-1
更新日期：2017-04-19 00:00:00
abstract：:Plant extracts, or phytonutrients, are used in traditional medicine practices as supplements to enhance the immune system and gain resistance to various infectious diseases and are used in animal production as health promoting feed additives. To date, there are no studies that have assessed their mechanism of action a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09253
更新日期：2015-03-19 00:00:00
abstract：:Recent studies demonstrate that the invasion and metastasis of gastric cancer (GC) is closely associated with a multi-subunit vacuolar H+-ATPase (V-ATPase). Here we investigated the expression and role of the human ATP6V1A gene that encodes the catalytic subunit A of V-ATPase in GC. We found that ATP6V1A expression le...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-03021-3
更新日期：2017-06-07 00:00:00
abstract：:Forest cutting is a major anthropogenic disturbance that affects forest carbon (C) storage and fluxes. Yet its characteristics and impacts on C cycling are poorly understood over large areas. Using recent annualized forest inventory data, we estimated cutting-related loss of live biomass in the eastern United States w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep03547
更新日期：2013-12-19 00:00:00
