Abstract：
:NF-E2-related factor 2 (Nrf2) has been identified as a master regulatory factor in the protection of cells from oxidative and electrophilic stress. However, overexpression of Nrf2 in lung cancer may cause chemoresistance, as well as radioresistance. In this study, we examined the relationship between radioresistance and Nrf2 protein levels in H1299, A549, and H460 cells, and finally chose the A549 cell line to continue with due to its strong radioresistance and high Nrf2 protein levels. We found that the Nrf2 inhibitor, brusatol, could prevent the increase and accumulation of Nrf2 after exposure to irradiation. Additionally, following treatment with 80 nM brusatol, A549 cells became sensitive to irradiation, suffering severe DNA damage. Combination treatment with brusatol and ionizing radiation (IR) can distinctly increase the level of reactive oxygen species in A549 cells, causing a 1.8-fold increase compared with the control, and a 1.4-fold increase compared with IR alone. In fact, in the treatment with both brusatol and IR, lung cancer cell proliferation is halted, gradually leading to cell death. Because Nrf2 is closely linked to DNA damage repair, inhibiting the function of Nrf2, as in brusatol treatment, may increase the DNA damage caused by radiotherapy or chemotherapy, possibly enhancing the efficacy of chemotherapeutic drugs. Our study is the first to demonstrate brusatol's ability to enhance the responsiveness of lung cancer cells to irradiation, and its potential application as a natural sensitizer in radiotherapy.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Sun X,Wang Q,Wang Y,Du L,Xu C,Liu Qdoi
10.3390/ijms17070997subject
Has Abstractpub_date
2016-06-24 00:00:00issue
7issn
1422-0067pii
ijms17070997journal_volume
17pub_type
杂志文章abstract：:The insect midgut secretes a semi-permeable, acellular peritrophic membrane (PM) that maintains intestinal structure, promotes digestion, and protects the midgut from food particles and pathogenic microorganisms. Peritrophin is an important PM protein (PMP) in the PM. Here, we identified 11 peritrophins with 1-16 chit...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21217973
更新日期：2020-10-27 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2020-11-10 00:00:00
abstract：:Polyglutamine diseases, including Huntington's disease and a number of spinocerebellar ataxias, are caused by expanded CAG repeats that are located in translated sequences of individual, functionally-unrelated genes. Only mutant proteins containing polyglutamine expansions have long been thought to be pathogenic, but ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms160818741
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2018-09-27 00:00:00
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journal_title：International journal of molecular sciences
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doi：10.3390/ijms17101604
更新日期：2016-09-22 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,随机对照试验
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2016-04-01 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18081722
更新日期：2017-08-07 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21218111
更新日期：2020-10-30 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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pub_type： 杂志文章,评审
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journal_title：International journal of molecular sciences
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更新日期：2020-01-16 00:00:00
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更新日期：2018-03-13 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2014-07-30 00:00:00
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2018-03-23 00:00:00
abstract：:Controlling the differentiation potential of adipose-derived stem cells (ADSCs) is attracting attention as a new strategy for the prevention and treatment of obesity. Here, we aimed to observe the effect of exercise training (TR) and high-fat diet (HFD) on the metabolic profiles of ADSCs-derived adipocytes. The rats w...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms22020966
更新日期：2021-01-19 00:00:00
