Profilin connects actin assembly with microtubule dynamics.

abstract：:The export of mRNA from the nucleus to the cytoplasm involves interactions of proteins with mRNA and the nuclear pore complex. We isolated Crp79p, a novel mRNA export factor from the same synthetic lethal screen that led to the identification of spMex67p in Schizosaccharomyces pombe. Crp79p is a 710-amino-acid-long pr...

journal_title：Molecular biology of the cell

authors： Thakurta AG,Whalen WA,Yoon JH,Bharathi A,Kozak L,Whiteford C,Love DC,Hanover JA,Dhar R

更新日期：2002-08-01 00:00:00

abstract：:Phosphatidylinositol transfer proteins (PITPs) regulate the interface between lipid metabolism and specific steps in membrane trafficking through the secretory pathway in eukaryotes. Herein, we describe the cis-acting information that controls PITPbeta localization in mammalian cells. We demonstrate PITPbeta localizes...

journal_title：Molecular biology of the cell

authors： Phillips SE,Ile KE,Boukhelifa M,Huijbregts RP,Bankaitis VA

更新日期：2006-06-01 00:00:00

abstract：:Sec13p has been thought to be an essential component of the COPII coat, required for exit of proteins from the yeast endoplasmic reticulum (ER). We show herein that normal function of Sec13p was not required for ER exit of the Hsp150 glycoprotein. Hsp150 was secreted to the medium under restrictive conditions in a sec...

journal_title：Molecular biology of the cell

authors： Fatal N,Suntio T,Makarow M

更新日期：2002-12-01 00:00:00

abstract：:The exocyst is an evolutionarily conserved octameric protein complex that tethers post-Golgi secretory vesicles at the plasma membrane for exocytosis. To elucidate the mechanism of vesicle tethering, it is important to understand how the exocyst physically associates with the plasma membrane (PM). In this study, we re...

journal_title：Molecular biology of the cell

authors： Liu J,Zuo X,Yue P,Guo W

更新日期：2007-11-01 00:00:00

abstract：:The small GTPase Ran is essential for spindle assembly. Ran is proposed to act through its nuclear import receptors importin alpha and/or importin beta to control the sequestration of proteins necessary for spindle assembly. To date, the molecular mechanisms by which the Ran pathway functions remain unclear. Using pur...

journal_title：Molecular biology of the cell

authors： Ems-McClung SC,Zheng Y,Walczak CE

更新日期：2004-01-01 00:00:00

abstract：:Mutations in the cytolinker protein plectin lead to grossly distorted morphology of neuromuscular junctions (NMJs) in patients suffering from epidermolysis bullosa simplex (EBS)-muscular dystrophy (MS) with myasthenic syndrome (MyS). Here we investigated whether plectin contributes to the structural integrity of NMJs ...

journal_title：Molecular biology of the cell

authors： Mihailovska E,Raith M,Valencia RG,Fischer I,Al Banchaabouchi M,Herbst R,Wiche G

更新日期：2014-12-15 00:00:00

abstract：:The RNA-binding protein HuD binds to a regulatory element in the 3' untranslated region (3' UTR) of the GAP-43 mRNA. To investigate the functional significance of this interaction, we generated PC12 cell lines in which HuD levels were controlled by transfection with either antisense (pDuH) or sense (pcHuD) constructs....

journal_title：Molecular biology of the cell

authors： Mobarak CD,Anderson KD,Morin M,Beckel-Mitchener A,Rogers SL,Furneaux H,King P,Perrone-Bizzozero NI

更新日期：2000-09-01 00:00:00

abstract：:Extracellular signal-regulated kinase (Erk) is widely recognized for its central role in cell proliferation and motility. Although previous work has shown that Erk is localized at endosomal compartments, no role for Erk in regulating endosomal trafficking has been demonstrated. Here, we report that Erk signaling regul...

journal_title：Molecular biology of the cell

authors： Robertson SE,Setty SR,Sitaram A,Marks MS,Lewis RE,Chou MM

更新日期：2006-02-01 00:00:00

abstract：:Alternative pre-mRNA splicing patterns can change an extracellular stimulus, but the signaling pathways leading to these changes are still poorly characterized. Here, we describe a tyrosine-phosphorylated nuclear protein, YT521-B, and show that it interacts with the nuclear transcriptosomal component scaffold attachme...

journal_title：Molecular biology of the cell

authors： Hartmann AM,Nayler O,Schwaiger FW,Obermeier A,Stamm S

更新日期：1999-11-01 00:00:00

abstract：:The expression of FREQUENCY, a central component of the circadian clock in Neurospora crassa, shows daily cycles that are exquisitely sensitive to the environment. Two forms of FRQ that differ in length by 99 amino acids, LFRQ and SFRQ, are synthesized from alternative initiation codons and the change in their ratio a...

journal_title：Molecular biology of the cell

authors： Colot HV,Loros JJ,Dunlap JC

更新日期：2005-12-01 00:00:00

abstract：:The RNA component of the signal recognition particle (SRP) is universally required for cotranslational protein targeting. Biochemical studies have shown that SRP RNA participates in the central step of protein targeting by catalyzing the interaction of the SRP with the SRP receptor (SR). SRP RNA also accelerates GTP h...

journal_title：Molecular biology of the cell

authors： Bradshaw N,Walter P

更新日期：2007-07-01 00:00:00

abstract：:Target of rapamycin complex 1 (TORC1) is a central cellular signaling coordinator that allows eukaryotic cells to adapt to the environment. In the budding yeast, Saccharomyces cerevisiae, TORC1 senses nitrogen and various stressors and modulates proteosynthesis, nitrogen uptake and metabolism, stress responses, and au...

journal_title：Molecular biology of the cell

authors： Takeda E,Jin N,Itakura E,Kira S,Kamada Y,Weisman LS,Noda T,Matsuura A

更新日期：2018-02-15 00:00:00

abstract：:Cytokinesis in metazoan cells requires a set of antiparallel microtubules that become bundled upon anaphase onset to form a structure known as the central spindle. Bundling of these microtubules requires a protein complex, centralspindlin, that consists of the CYK-4/MgcRacGAP Rho-family GTPase-activating protein and t...

journal_title：Molecular biology of the cell

authors： Pavicic-Kaltenbrunner V,Mishima M,Glotzer M

更新日期：2007-12-01 00:00:00

abstract：:Normal epithelial cells undergo apoptosis when they are denied contact with the extracellular matrix, in a process termed "anoikis." Conversely, malignant epithelial cells typically acquire anchorage independence, i.e., the capacity to survive and grow in the absence of matrix interaction. Here we asked the question w...

journal_title：Molecular biology of the cell

authors： Jost M,Huggett TM,Kari C,Rodeck U

更新日期：2001-05-01 00:00:00

abstract：:Rab2 immunolocalizes to pre-Golgi intermediates (vesicular-tubular clusters [VTCs]) that are the first site of segregation of anterograde- and retrograde-transported proteins and a major peripheral site for COPI recruitment. Our previous work showed that Rab2 Q65L (equivalent to Ras Q61L) inhibited endoplasmic reticul...

journal_title：Molecular biology of the cell

authors： Tisdale EJ

更新日期：1999-06-01 00:00:00

abstract：:Mutations in the Caenorhabditis elegans unc-84 gene cause defects in nuclear migration and anchoring. We show that endogenous UNC-84 protein colocalizes with Ce-lamin at the nuclear envelope and that the envelope localization of UNC-84 requires Ce-lamin. We also show that during mitosis, UNC-84 remains at the nuclear ...

journal_title：Molecular biology of the cell

authors： Lee KK,Starr D,Cohen M,Liu J,Han M,Wilson KL,Gruenbaum Y

更新日期：2002-03-01 00:00:00

abstract：:Shroom (Shrm) proteins are essential regulators of cell shape and tissue morpho-logy during animal development that function by interacting directly with the coiled-coil region of Rho kinase (Rock). The Shrm-Rock interaction is sufficient to direct Rock subcellular localization and the subsequent assembly of contracti...

journal_title：Molecular biology of the cell

authors： Mohan S,Rizaldy R,Das D,Bauer RJ,Heroux A,Trakselis MA,Hildebrand JD,VanDemark AP

更新日期：2012-06-01 00:00:00

abstract：:Cellular responses to environmental changes occur on different levels. We investigated the translational response of yeast cells after mild hyperosmotic shock by isolating mRNA associated with multiple ribosomes (polysomes) followed by array analysis. Globally, recruitment of preexisting mRNAs to ribosomes (translatio...

journal_title：Molecular biology of the cell

authors： Warringer J,Hult M,Regot S,Posas F,Sunnerhagen P

更新日期：2010-09-01 00:00:00

abstract：:A key question in eukaryotic differentiation is whether there are common regulators or biochemical events that are required for diverse types of differentiation or whether there is a core mechanism for differentiation. The unicellular model organism Saccharomyces cerevisiae undergoes filamentous differentiation in res...

journal_title：Molecular biology of the cell

authors： Jiang YW,Kang CM

更新日期：2003-12-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the Apg12p-Apg5p conjugating system is essential for autophagy. Apg7p is required for the conjugation reaction, because Apg12p is unable to form a conjugate with Apg5p in the apg7/cvt2 mutant. Apg7p shows a significant similarity to a ubiquitin-activating enzyme, Uba1p. In this a...

journal_title：Molecular biology of the cell

authors： Tanida I,Mizushima N,Kiyooka M,Ohsumi M,Ueno T,Ohsumi Y,Kominami E

更新日期：1999-05-01 00:00:00

abstract：:During the course of a bacterial infection, cells are exposed simultaneously to a range of bacterial and host factors, which converge on the central transcription factor nuclear factor (NF)-κB. How do single cells integrate and process these converging stimuli? Here we tackle the question of how cells process combinat...

journal_title：Molecular biology of the cell

authors： Gutschow MV,Mason JC,Lane KM,Maayan I,Hughey JJ,Bajar BT,Amatya DN,Valle SD,Covert MW

更新日期：2019-01-15 00:00:00

abstract：:Mitochondrial function depends crucially on the maintenance of multiple mitochondrial DNA (mtDNA) copies. Surprisingly, the cellular mechanisms regulating mtDNA copy number remain poorly understood. Through a systematic high-throughput approach in Saccharomyces cerevisiae, we determined mtDNA-to-nuclear DNA ratios in ...

journal_title：Molecular biology of the cell

authors： Göke A,Schrott S,Mizrak A,Belyy V,Osman C,Walter P

更新日期：2020-03-19 00:00:00

abstract：:Fibroblasts, when plated on the extracellular matrix protein fibronectin (FN), rapidly spread and form an organized actin cytoskeleton. This process is known to involve both the central alpha5beta1 integrin-binding and the C-terminal heparin-binding regions of FN. We found that within the heparin-binding region, the i...

journal_title：Molecular biology of the cell

authors： Bloom L,Ingham KC,Hynes RO

更新日期：1999-05-01 00:00:00

abstract：:The ARF tumor suppressor is a product of the INK4a/ARF locus, which is frequently mutated in human cancer. The expression of ARF is up-regulated in response to certain types of DNA damage, oncogene activation, and interferon stimuli. Through interaction with the p53 negative regulator MDM2, ARF controls a well-describ...

journal_title：Molecular biology of the cell

authors： Li Z,Hou J,Sun L,Wen T,Wang L,Zhao X,Xie Q,Zhang SQ

更新日期：2012-12-01 00:00:00

abstract：:Mitochondrial decline is a hallmark of aging, and cells are equipped with many systems to regulate mitochondrial structure and function in response to stress and metabolic alterations. Here, using budding yeast, we identify a proteolytic pathway that contributes to alterations in mitochondrial structure in aged cells ...

journal_title：Molecular biology of the cell

authors： Goodrum JM,Lever AR,Coody TK,Gottschling DE,Hughes AL

更新日期：2019-08-01 00:00:00

abstract：:A major goal of synthetic biology is to define the minimal cellular machinery required to assemble a biological structure in its simplest form. Here, we focused on Saccharomyces cerevisiae actin cables, which provide polarized tracks for intracellular transport and maintain defined lengths while continuously undergoin...

journal_title：Molecular biology of the cell

authors： Pollard LW,Garabedian MV,Alioto SL,Shekhar S,Goode BL

更新日期：2020-03-01 00:00:00

abstract：:Schizosaccharomyces pombe Rho GTPases regulate actin cytoskeleton organization and cell integrity. We studied the fission yeast gene SPBC4F6.12 based on its ability to suppress the thermosensitivity of cdc42-1625 mutant strain. This gene, named pxl1(+), encodes a protein with three LIM domains that is similar to paxil...

journal_title：Molecular biology of the cell

authors： Pinar M,Coll PM,Rincón SA,Pérez P

更新日期：2008-04-01 00:00:00

abstract：:Cyclin D1 expression is jointly regulated by growth factors and cell adhesion to the extracellular matrix in many cell types. Growth factors are thought to regulate cyclin D1 expression because they stimulate sustained extracellular signal-regulated kinase (ERK) activity. However, we show here that growth factors indu...

journal_title：Molecular biology of the cell

authors： Roovers K,Davey G,Zhu X,Bottazzi ME,Assoian RK

更新日期：1999-10-01 00:00:00

abstract：:In mouse embryoid bodies, mutation of the tight junction protein cingulin results in changes in gene expression. Here, we studied the function of cingulin using a gene silencing approach in Madin-Darby canine kidney (MDCK) cells. Cingulin-depleted cells show higher protein and mRNA levels of claudin-2 and ZO-3, increa...

journal_title：Molecular biology of the cell

authors： Guillemot L,Citi S

更新日期：2006-08-01 00:00:00

abstract：:Clustered protocadherins (Pcdhs) are arranged in gene clusters (α, β, and γ) with variable and constant exons. Variable exons encode cadherin and transmembrane domains and ~90 cytoplasmic residues. The 14 Pcdh-αs and 22 Pcdh-γs are spliced to constant exons, which, for Pcdh-γs, encode ~120 residues of an identical cyt...

journal_title：Molecular biology of the cell

authors： O'Leary R,Reilly JE,Hanson HH,Kang S,Lou N,Phillips GR

更新日期：2011-11-01 00:00:00