Abstract：
:In recent years it has been shown that the therapeutic benefits of human mesenchymal stem/stromal cells (hMSCs) in the Central Nervous System (CNS) are mainly attributed to their secretome. The implementation of computer-controlled suspension bioreactors has shown to be a viable route for the expansion of these cells to large numbers. As hMSCs actively respond to their culture environment, there is the hypothesis that one can modulate its secretome through their use. Herein, we present data indicating that the use of computer-controlled suspension bioreactors enhanced the neuroregulatory profile of hMSCs secretome. Indeed, higher levels of in vitro neuronal differentiation and NOTCH1 expression in human neural progenitor cells (hNPCs) were observed when these cells were incubated with the secretome of dynamically cultured hMSCs. A similar trend was also observed in the hippocampal dentate gyrus (DG) of rat brains where, upon injection, an enhanced neuronal and astrocytic survival and differentiation, was observed. Proteomic analysis also revealed that the dynamic culturing of hMSCs increased the secretion of several neuroregulatory molecules and miRNAs present in hMSCs secretome. In summary, the appropriate use of dynamic culture conditions can represent an important asset for the development of future neuro-regenerative strategies involving the use of hMSCs secretome.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Teixeira FG,Panchalingam KM,Assunção-Silva R,Serra SC,Mendes-Pinheiro B,Patrício P,Jung S,Anjo SI,Manadas B,Pinto L,Sousa N,Behie LA,Salgado AJdoi
10.1038/srep27791subject
Has Abstractpub_date
2016-06-15 00:00:00pages
27791issn
2045-2322pii
srep27791journal_volume
6pub_type
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