Abstract:
:Prostaglandin E2 (PGE2), a cyclooxygenase metabolite that generally acts as a systemic vasodepressor, has been shown to have vasopressor effects under certain physiologic conditions. Previous studies have demonstrated that PGE2 receptor signaling modulates angiotensin II (Ang II)-induced hypertension, but the interaction of these two systems in the regulation of vascular reactivity is incompletely characterized. We hypothesized that Ang II, a principal effector of the renin-angiotensin-aldosterone system, potentiates PGE2-mediated vasoconstriction. Here we demonstrate that pre-treatment of arterial rings with 1nM Ang II potentiated PGE2-evoked constriction in a concentration dependent manner (AUC-Ang II 2.778±2.091, AUC+Ang II 22.830±8.560, ***P<0.001). Using genetic deletion models and pharmacological antagonists, we demonstrate that this potentiation effect is mediated via concurrent signaling between the angiotensin II receptor 1 (AT1) and the PGE2 E-prostanoid receptor 3 (EP3) in the mouse femoral artery. EP3 receptor-mediated vasoconstriction is shown to be dependent on extracellular calcium in combination with proline-rich tyrosine kinase 2 (Pyk2) and Rho-kinase. Thus, our findings reveal a novel mechanism through which Ang II and PGE2 regulate peripheral vascular reactivity.
journal_name
Vascul Pharmacoljournal_title
Vascular pharmacologyauthors
Kraemer MP,Choi H,Reese J,Lamb FS,Breyer RMdoi
10.1016/j.vph.2016.05.015subject
Has Abstractpub_date
2016-09-01 00:00:00pages
47-54eissn
1537-1891issn
1879-3649pii
S1537-1891(16)30016-7journal_volume
84pub_type
杂志文章abstract::We recently demonstrated that lipoic acid suppresses endotoxin-stimulated expression of inducible nitric oxide synthase and nitric oxide production in mouse macrophages. In this study, we tested whether lipoic acid suppresses these inflammatory mediators in the lungs of rats. Rats were assigned to receive either no sp...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2005.08.005
更新日期:2005-12-01 00:00:00
abstract::We investigated the effects of epinephrine and dopamine on retinal blood vessels in streptozotocin (STZ, 80 mg/kg, i.p.)-treated rats and age-matched control rats to determine whether diabetes mellitus alters the retinal vascular responses to circulating catecholamines. Experiments were performed 6-8 weeks after treat...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2008.06.001
更新日期:2008-08-01 00:00:00
abstract::Endothelial cell dysfunction may play an important role in the development of various vascular diseases, including atherosclerosis. Here we investigated whether lithium chloride (LiCl), an inhibitor of glycogen synthase kinase-3β (GSK-3β), could counteract atherosclerosis induced by a high-fat diet in ApoE⁻/⁻ mice. Te...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2010.09.004
更新日期:2010-11-01 00:00:00
abstract::LIGHT is known to act as a novel mediator for atherogenesis. Furthermore, it has been reported that emodin, an active component extracted from rhubarb, can stop the growth of cancer cells. However, it is not known if emodin exerts anti-atherogenic effects in the human monocyte, THP-1, following treatment with LIGHT. I...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2010.03.002
更新日期:2010-07-01 00:00:00
abstract::Chemerin is an adipokine associated with increased blood pressure, and may link obesity with hypertension. We tested the hypothesis that chemerin-induced contraction of the vasculature occurs via calcium flux in smooth muscle cells. Isometric contraction of rat aortic rings was performed in parallel with calcium kinet...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2016.11.009
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Suboptimal platelet inhibition still represents an important challenge, especially for patients undergoing percutaneous coronary interventions (PCI). Chronic kidney disease (CKD) is a common comorbidity of patients with coronary artery disease, and may potentially influence platelet reactivity. So far only f...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2015.10.006
更新日期:2016-04-01 00:00:00
abstract:OBJECTIVE:In patients with familial combined hyperlipidemia (FCHL), without metabolic syndrome (MS), occurrence of non-alcoholic fatty liver disease (NAFLD) is related to a specific pro-inflammatory profile, influenced by genetic traits, involved in oxidative stress and adipokine secretion. Among FCHL or MS patients, h...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2015.04.004
更新日期:2015-09-01 00:00:00
abstract::Tyrosine kinase pathway has been shown to be involved in the effects of hypoxia in pulmonary arteries, but its role in pulmonary vein is not known. The aims of this study were to determine the effect of hypoxia in sheep isolated pulmonary veins and to identify the role of tyrosine kinase pathway in hypoxic response. G...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/s1537-1891(03)00051-x
更新日期:2003-10-01 00:00:00
abstract::Four main vascular effects of hydrogen peroxide (H2O2) were studied in intact and rubbed aortic rings from WKY rats. In rings partially precontracted with phenylephrine: 1-30 microM H2O2 induced an increase of tone, 100 microM H2O2 produced a transient contraction followed by a fast-developing endothelium-independent ...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2005.06.001
更新日期:2005-08-01 00:00:00
abstract::The key for the survival of all organisms is the regulation and control of energy metabolism. Thus, several strategies have evolved in each tissue in order to balance nutrient supply with energy demand. Adenosine monophosphate-activated protein kinase (AMPK) is now recognized as a key participant in energy metabolism....
journal_title:Vascular pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.vph.2015.02.017
更新日期:2015-04-01 00:00:00
abstract::Neurokinin(3) (NK(3)) receptors may regulate the airways primarily through actions on the nerves. In the periphery, airway parasympathetic ganglia neurons are depolarized following NK(3) receptor activation resulting subsequently in the facilitation of synaptic transmission. Such an effect may account for the excessiv...
journal_title:Vascular pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.vph.2005.08.031
更新日期:2006-10-01 00:00:00
abstract::Results of our previous studies have suggested that enhanced generation of superoxide (O2(-)) may contribute to the pathophysiology of hypertension in Ren-2 transgenic rats (TGR). The present study was performed to evaluate in TGR the effects of chronic treatment with the O2(-) scavenger tempol and the antioxidant apo...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2009.06.001
更新日期:2009-08-01 00:00:00
abstract::AMP-activated protein kinase (AMPK) is present in the arterial wall and is activated in response to cellular stressors that raise AMP relative to ADP/ATP. Activation of AMPK in vivo lowers blood pressure but the influence of hyperlipidemia on this response has not been studied. ApoE(-/-) mice on high fat diet for 6wee...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2015.07.010
更新日期:2015-11-01 00:00:00
abstract::Genetic mutations in bone morphogenetic protein receptor 2 (BMPR2) have been shown to occur in patients with familial and idiopathic pulmonary arterial hypertension (PAH). However the interactions between ligands for this receptor and other mediators implicated in heritable PAH have not been investigated. This study e...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2009.09.001
更新日期:2009-11-01 00:00:00
abstract::Peroxisome proliferator-activated receptors (PPAR)alpha, gamma and beta/delta belong to the nuclear receptor family of ligand-activated transcription factors. PPARs heterodimerize with the retinoid X receptor (RXR) and then act as transcription factors to modulate the function of many target genes. PPARalpha, gamma an...
journal_title:Vascular pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.vph.2005.11.014
更新日期:2006-07-01 00:00:00
abstract::The antiplatelet and antithrombotic activities of a newly synthesized CP201, 2-(3,5-di-tert-butyl-4-hydroxyl)-3-chloro-1,4-naphthoquinone on human platelet aggregation in vitro and murine pulmonary thrombosis in vivo were examined. In addition, the antiplatelet activity of CP201 involved in calcium-signaling cascade w...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2004.04.001
更新日期:2004-02-01 00:00:00
abstract::In this study, we have investigated the actions of danshensu, an active, water-extractable component of the medicinal herb danshen (Salvia miltiorrhiza), on rat isolated coronary artery rings precontracted with 1 microM 5-hydroxytryptamine (5-HT) and its action compared to the water-extractable fraction of the herb. E...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2006.10.011
更新日期:2007-04-01 00:00:00
abstract::Endothelin (ET-1) is chronically elevated in diabetes. However, role of ET-1 in increased oxidative stress in type 2 diabetes is less clear. This study tested the hypotheses that: 1) oxidative stress markers are increased and total antioxidant capacity is decreased in diabetes, and 2) activation of ET(A) receptors med...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2007.05.006
更新日期:2007-08-01 00:00:00
abstract::Recent studies evidence that peroxynitrite is spontaneously formed when nitric oxide (NO) and superoxide coexist and suggest that it is likely to be involved in the destruction of the pancreatic beta cells. We examined whether drugs that inhibit nitric oxide synthase (NOS) or scavenge peroxynitrite could abrogate STZ-...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/s1537-1891(02)00212-4
更新日期:2002-03-01 00:00:00
abstract::Autoimmune rheumatic diseases are characterised by systemic inflammation and complex immunopathology, with an increased risk of cardiovascular disease, initiated by endothelial dysfunction in a chronic inflammatory environment. Endothelial microparticles (EMPs) are released into the circulation from activated endothel...
journal_title:Vascular pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.vph.2016.05.016
更新日期:2016-11-01 00:00:00
abstract::Intra-aortic balloon pump (IABP) counterpulsation has been the most widely used left ventricular assist device for nearly five decades. Due to diastolic inflation and systolic deflation, coronary blood flow is increased and afterload decreased translating into augmentation of oxygen supply and lowering of oxygen deman...
journal_title:Vascular pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.vph.2013.12.003
更新日期:2014-02-01 00:00:00
abstract::Hypertension is a predominant risk factor for cardiovascular diseases and a major health care burden. Accumulating epidemiological and experimental evidence suggest that adult-onset hypertension may have its origins during early development. Upon exposure to glucocorticoids, the fetus develops hypertension, and the of...
journal_title:Vascular pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.vph.2016.02.002
更新日期:2016-07-01 00:00:00
abstract::Therapeutic modulation of angiogenesis is believed to be a prospective powerful treatment strategy to modulate the microcirculation and therefore help millions of patients with cardiovascular and cancer diseases. The often-frustrating results from late-stage clinical studies indicate an urgent need for improved assess...
journal_title:Vascular pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.vph.2018.09.003
更新日期:2019-01-01 00:00:00
abstract::Excessive iron can generate reactive oxygen species (ROS), leading to oxidative stress that is closely associated with cardiovascular dysfunction. Iron overload was induced in male ICR mice by injection of iron sucrose (10mg/kg/day) for eight weeks. Iron overload was evidenced by increased serum iron indices. The mice...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2016.10.001
更新日期:2016-12-01 00:00:00
abstract::Animal studies have identified monocyte chemoattractive protein-1 (MCP-1) and vascular endothelial growth factor (VEGF) as critical mediators of arterial diameter enlargement in response to chronic increases in blood flow (arteriogenesis). Furthermore, cellular studies have shown that the shear stresses resulting from...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2008.11.005
更新日期:2009-03-01 00:00:00
abstract::The sulfur-containing amino acid, taurine, has been shown to ameliorate the vascular disorders. We examined the effects of taurine ingestion on intimal thickening following balloon injury. Balloon injury was induced in the left common carotid artery of Wistar rats. Taurine (3% (w/v)) was mixed in the drinking water fr...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2010.07.003
更新日期:2010-09-01 00:00:00
abstract:BACKGROUND:Proprotein convertase subtilisin-kexin type 9 inhibitors (PCSK9-I) reduce low-density lipoprotein (LDL) cholesterol in human studies. Previous studies suggest that PCSK9-I may also affect very-low-density lipoproteins (VLDL). We therefore studied VLDL size and composition in a "real-world" study population w...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2019.03.002
更新日期:2019-05-01 00:00:00
abstract::Metformin (Met) can improve atherosclerosis (As). Abnormal endothelin receptors [including endothelin type A (ETA) or type B (ETB) receptor] in vascular smooth muscle cells (VSMCs) are involved in As. Hyperhomocysteinemia (HHcy) is an independent risk factor for As. The present study was designed to test our hypothesi...
journal_title:Vascular pharmacology
pub_type: 杂志文章
doi:10.1016/j.vph.2019.106613
更新日期:2020-01-01 00:00:00
abstract::Impaired endothelial function is recognised as one of the earliest events of atherogenesis. Endothelium-dependent vasomotion has been the principal method to assess endothelial function. In this article, we will discuss the clinical value of the different techniques to evaluate endothelium-dependent vasomotion. To dat...
journal_title:Vascular pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.vph.2005.01.009
更新日期:2005-02-01 00:00:00
abstract::Atherosclerosis is a complex multifactorial disease that affects large and medium-sized arteries. Rupture of atherosclerotic plaques and subsequent acute cardiovascular complications remain a leading cause of death and morbidity in the Western world. There is a considerable difference in safety profile between a stabl...
journal_title:Vascular pharmacology
pub_type: 杂志文章,评审
doi:10.1016/j.vph.2018.06.014
更新日期:2019-01-01 00:00:00