Characterization of cholinesterase molecular forms in the mucosal cells along the intestine of the chicken.

Abstract:

:The presence of a butyrylcholinesterase (BuChE, EC 3.1.1.8) in the musocal cells of the chicken intestine was demonstrated by histochemical and biochemical methods. The study of its distribution, along the intestine from duodenum to rectum, showed that the jejuno-ileum possesses the highest activity. Sucrose gradient centrifugation revealed, in all intestinal areas, two globular forms with sedimentation coefficients of 4.3 S (G1 form) and 10.8 S (G4 form). The presence of Triton X-100 in the preparations did not modify the sedimentation profiles of these two forms which can be considered as soluble BuChE. The ratio of G1/G4-forms progressively decreases along the intestine from duodenum to rectum indicating a predominance of the G4 form in the areas where the activity is low. Our results are discussed in relation to other studies of globular forms of chicken BuChE.

journal_name

Mol Cell Biochem

authors

Sine JP,Ferrand R,Colas B

doi

10.1007/BF00223513

subject

Has Abstract

pub_date

1989-01-23 00:00:00

pages

49-56

issue

1

eissn

0300-8177

issn

1573-4919

journal_volume

85

pub_type

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