Sex determination and gender expression: Reproductive investment in snails.

abstract：:Production of transgenic cattle by microinjection of DNA has been difficult and costly. To explore an alternative method, one- to four-cell bovine embryos were exposed to a replication-defective retrovirus by microinjection of retrovirus producer cells into the perivitelline space. Embryos were cultured in vitro for 3...

journal_title：Molecular reproduction and development

authors： Haskell RE,Bowen RA

更新日期：1995-03-01 00:00:00

abstract：:The gonadotropin releasing hormone (GnRH) has long been considered as a neuropeptide involved in the control of the reproductive cycle. However, the presence of GnRH and its receptors in various tissues, including ovary and testis, suggests a role as autocrine/paracrine factor. In the present study, we report the expr...

journal_title：Molecular reproduction and development

authors： Uzbekova S,Ferrière F,Guiguen Y,Bailhache T,Breton B,Lareyre JJ

更新日期：2001-05-01 00:00:00

abstract：:The purpose of the present study was to investigate the effects of prostaglandin (PG) E(2) and tumor necrosis factor (TNF) alpha on expression of vascular endothelial growth factor (VEGF) and its receptors, fms-like tyrosine kinase (Flt-1) and fetal liver kinase-1/kinase insert domain-containing receptor (Flk-1/KDR), ...

journal_title：Molecular reproduction and development

authors： Kowalczyk AE,Kaczmarek MM,Schams D,Ziecik AJ

更新日期：2008-10-01 00:00:00

abstract：:Acrosomal reaction is an essential prerequisite to fertilization. The changes in lipid composition of sperm membranes cause fusion of the plasma and outer acrosomal membranes that results in the exocytosis of acrosomal contents. We report that both bull and rabbit spermatozoa contain a phosphatidylcholine-specific pho...

journal_title：Molecular reproduction and development

authors： Hinkovska-Galchev V,Srivastava PN

更新日期：1992-11-01 00:00:00

abstract：:During implantation in mice, tissue inhibitor of matrix metalloproteinases-3 is believed to play a key role in inhibiting matrix metalloproteinase activity associated with embryo invasion and tissue remodeling. The first objective of this study was to quantitatively compare the steady-state mRNA levels of tissue inhib...

journal_title：Molecular reproduction and development

authors： Bany BM,Schultz GA

更新日期：2001-06-01 00:00:00

abstract：:Acrosin (ACR), a serine proteinase located in the acrosome of the sperm, has been presumed to be involved in the recognition and binding of the sperm to the zona pellucida of the ovum and the sperm penetration through the zona pellucida. To examine the function of acrosin in vivo, we have generated mice carrying a mut...

journal_title：Molecular reproduction and development

authors： Adham IM,Nayernia K,Engel W

更新日期：1997-03-01 00:00:00

abstract：:Germ cells require communication with associated somatic cells for normal gametogenesis, as exemplified by an oocyte that interacts with granulosa cells via paracrine factors as well as gap junctions located at sites of contact between these two cell types. The objective of the present study was to define the mechanis...

journal_title：Molecular reproduction and development

authors： McGinnis LK,Kinsey WH

更新日期：2015-02-01 00:00:00

abstract：:The morphological analysis of the spermatozoa from fertile and infertile men was performed using light and electron microscopy to clarify the relationship between sperm morphology and fertility. Semen samples obtained from 22 partners of pregnant women were prepared according to the protocol standardized in an interna...

journal_title：Molecular reproduction and development

authors： Kubo-Irie M,Matsumiya K,Iwamoto T,Kaneko S,Ishijima S

更新日期：2005-01-01 00:00:00

abstract：:The neomycin-resistant gene (neo(r)) is probably the most commonly used selectable marker gene in gene targeting and gene transfection research. In this study, the neo(r) gene construct was introduced into in vitro cultured goat foetal fibroblast cells (IV-5), and the cells were selected with 900 microg/ml G418. The G...

journal_title：Molecular reproduction and development

authors： Zou X,Wang Y,Cheng Y,Yang Y,Ju H,Tang H,Shen Y,Mu Z,Xu S,Du M

更新日期：2002-02-01 00:00:00

abstract：:Under the influence of progesterone, the porcine uterus synthesizes plasmin/trypsin inhibitor (PTI), a low molecular weight protein (M(r) approximately 14,000) belonging to the Kunitz family of proteinase inhibitors. Here it is demonstrated that mRNA for the same protein is produced by the developing trophoblast durin...

journal_title：Molecular reproduction and development

authors： Duffy JY,Stallings-Mann ML,Trout WE,Roberts RM

更新日期：1997-04-01 00:00:00

abstract：:We report the primary structures of two mRNA species (GH1 and GH2), each predicted from the cloned cDNA and genomic gene sequences, that encode growth hormone in rainbow trout (Salmo gairdneri). Both GH1 and GH2 mRNA contain open reading frames comprising 630 nucleotides and encode 210 amino acid residues, of which 11...

journal_title：Molecular reproduction and development

authors： Agellon LB,Davies SL,Lin CM,Chen TT,Powers DA

更新日期：1988-01-01 00:00:00

abstract：:The objective of this study was to examine the effects of colcemid treatment during oocyte in vitro maturation (IVM) and embryonic development after parthenogenetic activation (PA) and somatic-cell nucleus transfer (SCNT) in pigs. Immature oocytes were treated with colcemid from 0 to 22, 38 to 42, or 0 to 22 hr follow...

journal_title：Molecular reproduction and development

authors： Lee J,Park JI,Lee GS,Choi JH,Lee ST,Park CK,Kim DY,Hyun SH,Lee E

更新日期：2015-06-01 00:00:00

abstract：:The vascular cell responses to the type 1, 2, and 3 isoforms of transforming growth factor-beta (TGF-beta 1, TGF-beta 2, TGF-beta 3) were studied using bovine aortic endothelial (BAECs) and smooth muscle cells (BASMC3) as well as rat epididymal fat pad microvascular endothelia (RFCs). Three distinct bioassays indicate...

journal_title：Molecular reproduction and development

authors： Madri JA,Bell L,Merwin JR

更新日期：1992-06-01 00:00:00

abstract：:RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This is probably due to difficulty in introducing RNAi-inducing molecules, such as a short-hairpin RNA (shRNA) gene, into COCs by comm...

journal_title：Molecular reproduction and development

authors： Sugiura K,Su YQ,Eppig JJ

更新日期：2009-06-01 00:00:00

abstract：:Members of the Pumilio (Pum) family of RNA-binding proteins act as translational repressors and are required for germ cell development and asymmetric division. We identified the chicken Pum1 and Pum2 genes and analyzed their expression patterns in various tissues. Comparative sequence analysis of the Pum1 and Pum2 pro...

journal_title：Molecular reproduction and development

authors： Lee JY,Lim JM,Kim DK,Zheng YH,Moon S,Han BK,Song KD,Kim H,Han JY

更新日期：2008-01-01 00:00:00

abstract：:Oocyte maturation is accompanied by differentiation of surrounding cumulus cells. These cells produce hyaluronic acid (HA) and its storage in intercellular spaces results in expansion of the cells. The cumulus cells also accumulate cyclooxygenase-2 (cox-2) during maturation. Both expansion and cox-2 storage are regula...

journal_title：Molecular reproduction and development

authors： Vigneron C,Nuttinck F,Perreau C,Reinaud P,Charpigny G,Mermillod P

更新日期：2003-05-01 00:00:00

abstract：:The text below is a transcript of the keynote address Kenneth Manning gave at the symposium honoring E. E. Just that was held on the campus of Howard University on November 21, 2008. In his talk, Manning reflects on his experiences researching and writing Black Apollo of Science: The Life of Ernest Everett Just, his p...

journal_title：Molecular reproduction and development

authors： Manning KR

更新日期：2009-10-01 00:00:00

abstract：:Apoptosis is a well-established cellular mechanism for selective cell deletion during development. However, little is known about the expression of an apoptotic pathway and its role in determining the relative sensitivity of the early, pre-gastrula, avian embryo to stress-induced cell death. We examined the sensitivit...

journal_title：Molecular reproduction and development

authors： Muscarella DE,Rachlinski MK,Bloom SE

更新日期：1998-10-01 00:00:00

abstract：:Dickkopf-like 1 (DkkL1) is related to the Dickkopf gene family, a group of proteins that are characterized as secreted antagonists of Wingless (Wnt) signal transduction proteins. DkkL1 mRNA is found in preimplantation mouse embryos and in developing neural tissue, but in adults it is found primarily in the testes. In ...

journal_title：Molecular reproduction and development

authors： Kohn MJ,Kaneko KJ,DePamphilis ML

更新日期：2005-08-01 00:00:00

abstract：:The present study has used methoxyacetic acid (MAA)-induced depletion of specific germ cell types in the rat and in situ hybridization with nonradioactive riboprobes to determine the stages of the spermatogenic cycle at which there is expression of the mRNA for the basic chromosomal protein transition protein 2 (TP2)....

journal_title：Molecular reproduction and development

authors： Saunders PT,Millar MR,Maguire SM,Sharpe RM

更新日期：1992-12-01 00:00:00

abstract：:Ovulated rat oocytes undergo spontaneous activation during in vitro culture. After extrusion of the second polar body, they do not enter interphase but are arrested again in next metaphase-like stage (M III arrest). The present study demonstrates that puromycin and chloral hydrate can trigger transition to interphase ...

journal_title：Molecular reproduction and development

authors： Zernicka-Goetz M

更新日期：1991-02-01 00:00:00

abstract：:The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...

journal_title：Molecular reproduction and development

authors： Kasinsky HE,Frehlick LJ,Su HW,Ausio J

更新日期：2005-03-01 00:00:00

abstract：:Germinal vesicle (GV) breakdown in mammalian oocytes is regulated by the activation of maturation promoting factor (MPF). We investigated a specific cdc2 kinase inhibitor, roscovitine, to maintain pig oocytes in the GV stage. Cumulus-oocyte complexes (COCs) were aspirated from slaughterhouse ovaries and cultured for 4...

journal_title：Molecular reproduction and development

authors： Ju JC,Tsay C,Ruan CW

更新日期：2003-04-01 00:00:00

abstract：:To investigate the decrease of transcription and to determine the localization of RNA molecules during spermiogenesis in the fern Scolopendrium, we have used the enzyme-gold electron microscope method. During cell differentiation, the labeling decreases over the cytoplasm and increases over the nucleus. In the latter,...

journal_title：Molecular reproduction and development

authors： Rejon E,Bajon C,Blaize A,Robert D

更新日期：1988-01-01 00:00:00

abstract：:Sex-related growth rate differences in preimplantation mouse embryos were investigated. In experiment I, Day 3 embryos were recovered from reproductive tracts, classified according to developmental stage, and cultured for 24 hr in CZB medium containing glucose. Each embryo was then reclassified and stained for measure...

journal_title：Molecular reproduction and development

authors： Peippo J,Bredbacka P

更新日期：1995-01-01 00:00:00

abstract：:A group of low Mr (16 kDa-23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasm...

journal_title：Molecular reproduction and development

authors： Parry RV,Barker PJ,Jones R

更新日期：1992-09-01 00:00:00

abstract：:Basigin, a transmembrane glycoprotein belonging to the immunoglobulin superfamily, has been shown to be essential for fertilization and implantation. The aim of this study was to determine the expression and hormonal regulation of basigin gene in mouse uterus during the peri-implantation period. Basigin immunostaining...

journal_title：Molecular reproduction and development

authors： Xiao LJ,Chang H,Ding NZ,Ni H,Kadomatsu K,Yang ZM

更新日期：2002-09-01 00:00:00

abstract：:An exogenous supply of hematopoietic cytokines is essential for maintaining murine embryonic stem (ES) cells in a proliferative yet undifferentiated state. Recently, it was demonstrated that hematopoietic cytokines utilize the gp130 signal transduction pathway to maintain this phenotype, although their involvement tow...

journal_title：Molecular reproduction and development

authors： Moore K,Piedrahita JA

更新日期：1996-10-01 00:00:00

abstract：:Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related ...

journal_title：Molecular reproduction and development

authors： Ma Q,Wang H,Guo R,Wang H,Ge Y,Ma J,Xue S,Han D

更新日期：2006-09-01 00:00:00

abstract：:Lipopolysaccharide (LPS) induces germ cell apoptosis, but its mechanism of action is not clear. One possibility is that LPS regulates the expression of FAS ligand (FASLG) in Sertoli cells, which will then influence germ cell apoptosis. In this study, LPS reduced the viability of cultured, immature boar Sertoli cells i...

journal_title：Molecular reproduction and development

authors： Wang Y,Zhang JJ,Yang WR,Luo HY,Zhang JH,Wang XZ

更新日期：2015-11-01 00:00:00