Abstract:
:This paper presents the correlative imaging of collagen denaturation by nonlinear optical microscopy (NLO) and nanoscale infrared (IR) spectroscopy to obtain morphological and chemical information at different length scales. Such multiscale correlated measurements are applied to the investigation of ancient parchments, which are mainly composed of dermal fibrillar collagen. The main issue is to characterize gelatinization, the ultimate and irreversible alteration corresponding to collagen denaturation to gelatin, which may also occur in biological tissues. Key information about collagen and gelatin signatures is obtained in parchments and assessed by characterizing the denaturation of pure collagen reference samples. A new absorbing band is observed near the amide I band in the IR spectra, correlated to the onset of fluorescence signals in NLO images. Meanwhile, a strong decrease is observed in Second Harmonic signals, which are a structural probe of the fibrillar organization of the collagen at the micrometer scale. NLO microscopy therefore appears as a powerful tool to reveal collagen degradation in a non-invasive way. It should provide a relevant method to assess or monitor the condition of collagen-based materials in museum and archival collections and opens avenues for a broad range of applications regarding this widespread biological material.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Latour G,Robinet L,Dazzi A,Portier F,Deniset-Besseau A,Schanne-Klein MCdoi
10.1038/srep26344subject
Has Abstractpub_date
2016-05-19 00:00:00pages
26344issn
2045-2322pii
srep26344journal_volume
6pub_type
杂志文章abstract::Gene targeting in embryonic stem cells (ESCs) has become the principal technology for generating knockout models. Although numerous studies have predicted that the disruption of p53 leads to increased developmental anomalies and malignancies, most p53 knockout mice develop normally. Therefore, the role of p53 in anima...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep00945
更新日期:2012-01-01 00:00:00
abstract::A number of studies have suggested that the irradiation behavior and damage processes occurring during sequential and simultaneous particle irradiations can significantly differ. Currently, there is no definite answer as to why and when such differences are seen. Additionally, the conventional multi-particle irradiati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30191
更新日期:2016-07-28 00:00:00
abstract::Core-shell Nb2O5/TiO2 composite with hierarchical heterostructure is successfully synthesized In-situ by a facile template-free and acid-free solvothermal method based on the mechanism of liquid phase epitaxy. The chemical circumstance change induced by the alcoholysis of NbCl5 is utilized tactically to trigger core-s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25260
更新日期:2016-04-28 00:00:00
abstract::Variability in the x-ray tube current used in computed tomography may affect quantitative features extracted from the images. To investigate these effects, we scanned the Credence Cartridge Radiomics phantom 12 times, varying the tube current from 25 to 300 mA∙s while keeping the other acquisition parameters constant....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20713-6
更新日期:2018-02-05 00:00:00
abstract::The interaction between cellulase enzymes and their substrates is of central importance to several technological and scientific challenges. Here we report that the binding of cellulose binding modules (CBM) from Trichoderma reesei cellulases Cel6A and Cel7A show a major difference in how they interact with substrates ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35358
更新日期:2016-10-17 00:00:00
abstract::The attenuated Sabin strains contained in the oral poliomyelitis vaccine are genetically unstable, and their circulation in poorly immunized populations can lead to the emergence of pathogenic circulating vaccine-derived polioviruses (cVDPVs). The recombinant nature of most cVDPV genomes and the preferential presence ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38831
更新日期:2016-12-13 00:00:00
abstract::Radix Astragali is commonly used in traditional Chinese medicine, and its quality is closely related to ecological factors, such as climate and soil, in the production area. To provide high-quality Radix Astragali to Chinese and foreign markets, we used maximum entropy model and statistical analysis method, combined w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69391-3
更新日期:2020-07-27 00:00:00
abstract::The performance of most error-correction (EC) algorithms that operate on genomics reads is dependent on the proper choice of its configuration parameters, such as the value of k in k-mer based techniques. In this work, we target the problem of finding the best values of these configuration parameters to optimize error...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52196-4
更新日期:2019-11-06 00:00:00
abstract::Biomass waste treatment and detrimental dye adsorption are two of the crucial environmental issues nowadays. In this study, we investigate to simultaneously resolve the aforementioned issues by synthesizing chitosan sponges as adsorbents toward rose bengal (RB) dye adsorption. Through a temperature-controlled freeze-c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-18302-0
更新日期:2017-12-22 00:00:00
abstract::A cerebral aneurysm occurs as a result of a weakened blood vessel, which allows blood to flow into a sac or a ballooned section. Recent advancement shows that a new device, 'flow-diverter', can divert blood flow away from the aneurysm sac. People found that a flow-diverter based on thin film nitinol (TFN), works very ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23698
更新日期:2016-03-24 00:00:00
abstract::Precision medicine is continuously demanding for novel point of care systems, potentially exploitable also for in-vivo analysis. Biosensing probes based on Lab-On-Fiber Technology have been recently developed to meet these challenges. However, devices exploiting standard label-free approaches (based on ligand/target m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14852-5
更新日期:2017-10-31 00:00:00
abstract::Accurate intraocular lens (IOL) power calculation is always a challenge in ophthalmology, and unoptimized process may lead to inaccurate refractive outcomes. Quality control circle (QCC) has shown its success in many fields as a process management tool. However, its efficacy in ophthalmology remains unclear. Here we u...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14171-9
更新日期:2017-11-01 00:00:00
abstract::Intensive research has been focused on the synthesis of long-lasting SrAl2O4:EuDy in luminescent materials field. Traditionally, SrAl2O4:EuDy is synthesized in bulk form by solid state. However, their development remains restrained due to this technique is not compatible with large-scale production, sustainability and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00541-w
更新日期:2017-03-28 00:00:00
abstract::The aim of this study was to evaluate and compare the diagnostic accuracy, the inter-rater agreement and raters' certainty of cone beam computed tomography (CBCT) and radiography for the detection of scaphoid fractures. Our hypothesis is that the CBCT has a higher diagnostic accuracy for scaphoid fractures than radiog...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-22331-8
更新日期:2018-03-02 00:00:00
abstract::The renin-angiotensin system is known to regulate blood pressure as well as water- and electrolyte balance. An activated RAS is involved in the development of hypertension and hypertension-related organ damage. Thus, inhibitors of the RAS are protective and markedly increasing the life span of patients. In contrast, r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76712-z
更新日期:2020-11-12 00:00:00
abstract::Pyoverdine (PVDI) has been reported to act both as a siderophore for scavenging iron (a key nutrient) and a signaling molecule for the expression of virulence factors. This compound is itself part of a core set of virulence factors produced by Pseudomonas aeruginosa during infections. Once secreted into the bacterial ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56913-x
更新日期:2020-01-10 00:00:00
abstract::In neuroblastoma (NB), one of the most common paediatric solid tumours, activation of anaplastic lymphoma kinase (ALK) is often associated with poor outcomes. Although genetic studies have identified copy number alteration and nonsynonymous mutations of ALK, the regulatory mechanism of ALK signalling at protein levels...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32682
更新日期:2016-09-08 00:00:00
abstract::Abnormal dopaminergic transmission has been implicated as a risk determinant of HIV-1-associated neurocognitive disorders. HIV-1 Tat protein increases synaptic dopamine (DA) levels by directly inhibiting DA transporter (DAT) activity, ultimately leading to dopaminergic neuron damage. Through integrated computational m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39048
更新日期:2016-12-14 00:00:00
abstract::Intestinal microbiome changes that occur in HIV positive individuals on different antiretroviral therapy (ART) regimens are important to understand, as they are potentially linked with chronic inflammation and microbiome-linked comorbidities that occur at increased incidence in this population. We conducted a cross-se...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43741
更新日期:2017-03-06 00:00:00
abstract::Hetero-nanostructures based on magnetic contrast oxides have been prepared as highly dense nanoconsolidates. Cobalt ferrite-cobalt oxide core-shell type nanoparticles (NPs) were synthesized by seed mediated growth in polyol and subsequently consolidated by Spark Plasma Sintering (SPS) at 500 °C for a few minutes while...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55649-y
更新日期:2019-12-19 00:00:00
abstract::The mitogen-activated protein kinase (MAPK) cascades, which play crucial roles in plant development processes, are universal modules of signal transduction in eukaryotes and consist of a core module of three sequentially phosphorylated kinases: MAPK, MAPK kinase (MAPKK), and MAPKK kinase (MAPKKK). This is the first re...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17615-4
更新日期:2017-12-18 00:00:00
abstract::Brønsted-Lewis acidic ionic liquids (ILs) were applied to catalyze cellobiose to prepare levulinic acid (LA) in one pot under hydrothermal conditions. Under the optimum conditions, the highest LA yield of 67.51% was obtained when low [HO3S-(CH2)3-mim]Cl-FeCl3 (molar fraction of FeCl3 x = 0.60) was used. This indicated...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-38051-y
更新日期:2019-02-12 00:00:00
abstract::Liver segmentation is an essential procedure in computer-assisted surgery, radiotherapy, and volume measurement. It is still a challenging task to extract liver tissue from 3D CT images owing to nearby organs with similar intensities. In this paper, an automatic approach integrating multi-dimensional features into gra...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28787-y
更新日期:2018-07-16 00:00:00
abstract::We aimed to explore the effects of type-2 diabetes mellitus (T2DM) and hypoglycemic therapy on the salivary microbiome in periodontitis patients and identify the potential salivary micro-biomarker for the early warning of T2DM. Saliva samples were collected from healthy individuals (Health), periodontitis patients (P)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72035-1
更新日期:2020-09-21 00:00:00
abstract::Inflammatory and cardiovascular biomarkers have been associated with obesity, but little is known about how they change upon dietary intervention and concomitant weight loss. Further, protein biomarkers might be useful for predicting weight loss in overweight and obese individuals. We performed secondary analyses in t...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-020-64636-7
更新日期:2020-05-13 00:00:00
abstract::During interpersonal interactions, people perform actions with different forms of vitality, communicating their positive or negative attitude toward others. For example, a handshake can be "soft" or "vigorous", a caress can be 'kind' or 'rushed'. While previous studies have shown that the dorso-central insula is a key...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-73728-3
更新日期:2020-10-09 00:00:00
abstract::Experiments on the Ultimatum Game (UG) repeatedly show that people's behaviour is far from rational. In UG experiments, a subject proposes how to divide a pot and the other can accept or reject the proposal, in which case both lose everything. While rational people would offer and accept the minimum possible amount, i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19503-x
更新日期:2018-01-19 00:00:00
abstract::Recent reports have demonstrated the role of phyto-constituents in modulating inflammatory responses. Mangiferin isolated from Mangifera indica is known to induce potent anti-oxidative, anti-diabetic and anti-inflammatory activity. However, the molecular mechanism of its anti-inflammatory activity is not properly unde...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42683
更新日期:2017-02-20 00:00:00
abstract::In recent years, microbial cholesterol oxidases have gained great attention due to its widespread use in medical applications for serum cholesterol determination. Streptomyces aegyptia strain NEAE-102 exhibited high level of extracellular cholesterol oxidase production using a minimum medium containing cholesterol as ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20786-3
更新日期:2018-02-09 00:00:00
abstract::The aim of the study was to investigate the role of endogenous sulfur dioxide (SO2)/ aspartate aminotransferase 1 (AAT1) pathway in stretch-induced excessive collagen expression and its mechanism. The mechanical stretch downregulated SO2/AAT1 pathway and increased collagen I and III protein expression. Importantly, AA...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21112
更新日期:2016-02-16 00:00:00