LPS Induces Hyper-Permeability of Intestinal Epithelial Cells.

Abstract:

:Necrotizing Enterocolitis (NEC) is a severe inflammatory disorder leading to high morbidity and mortality rates. A growing body of evidence demonstrate the key role of the Toll like receptor 4 (TLR4) in NEC. This membranal receptor recognizes lipopolysaccharides (LPS) from the bacterial wall and triggers an inflammatory response. The aim of the present study was to elucidate the effect of LPS on paracellular permeability known to be severely affected in NEC. IEC-18 cells were treated with LPS and the effects on morphology, paracellular permeability and their associated gene and protein expressions were measured. Our results show that LPS down regulated the expression of occludin and ZO-1 mRNAs while up regulating Cdkn1a. In addition LPS caused a significant increase in paracellular permeability and epithelial barrier damage. Finally ZO-1 protein was found to be spatially disarrayed in the intercellular junctions in response to LPS. We conclude that LPS adversely affected the functionality of the intestinal epithelial barrier suggesting a new mechanism by which bacterial infection may contribute to the development of NEC. J. Cell. Physiol. 232: 381-390, 2017. © 2016 Wiley Periodicals, Inc.

journal_name

J Cell Physiol

authors

Bein A,Zilbershtein A,Golosovsky M,Davidov D,Schwartz B

doi

10.1002/jcp.25435

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

381-390

issue

2

eissn

0021-9541

issn

1097-4652

journal_volume

232

pub_type

杂志文章
  • Mechanisms of alpha-thrombin, histamine, and bradykinin induced endothelial permeability.

    abstract::alpha-Thrombin, bradykinin, and histamine are endogenous mediators that increase endothelial permeability. We examined the mechanism by which these three vasoactive mediators could alter permeability to albumin of human umbilical vein endothelial cells (HUVEC). HUVEC were grown to confluence on Transwell membranes and...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199606)167:3<562::AID-JCP2

    authors: Ehringer WD,Edwards MJ,Miller FN

    更新日期:1996-06-01 00:00:00

  • GP130/OSMR is the only LIF/IL-6 family receptor complex to promote osteoblast differentiation of calvaria progenitors.

    abstract::Leukemia inhibitory factor (LIF) and its receptor (LIFR) are "twins" of Oncostatin M (OSM) and OSMR, respectively, likely having arisen through gene duplications. We compared their effects in a bone nodule-forming model of in vitro osteogenesis, rat calvaria (RC) cell cultures. Using a dominant-negative LIF mutant (hL...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20312

    authors: Malaval L,Liu F,Vernallis AB,Aubin JE

    更新日期:2005-08-01 00:00:00

  • Combined treatment with systemic resveratrol and resveratrol preconditioned mesenchymal stem cells, maximizes antifibrotic action in diabetic cardiomyopathy.

    abstract::Wnt/β-catenin signaling pathway plays a crucial role in diabetic cardiomyopathy (DCM), thus we aimed at investigating the effect of one therapeutic approach with resveratrol (RSV) given systemically and combined treatment of RSV with mesenchymal stem cells (MSCs) that was either RSV-preconditioned or not on Wnt/β-cate...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27947

    authors: ShamsEldeen AM,Ashour H,Shoukry HS,Fadel M,Kamar SS,Aabdelbaset M,Rashed LA,Ammar HI

    更新日期:2019-07-01 00:00:00

  • The possible role of bacteria, viruses, and parasites in initiation and exacerbation of irritable bowel syndrome.

    abstract::Irritable bowel syndrome (IBS) is a prolonged and disabling functional gastrointestinal disorder with the incidence rate of 18% in the world. IBS could seriously affect lifetime of patients and cause high economic burden on the community. The pathophysiology of the IBS is hardly understood, whereas several possible me...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.27828

    authors: Shariati A,Fallah F,Pormohammad A,Taghipour A,Safari H,Chirani AS,Sabour S,Alizadeh-Sani M,Azimi T

    更新日期:2019-06-01 00:00:00

  • Frizzled-4 is required for normal bone acquisition despite compensation by Frizzled-8.

    abstract::The activation of the Wnt/β-catenin signaling pathway is critical for skeletal development but surprisingly little is known about the requirements for the specific frizzled (Fzd) receptors that recognize Wnt ligands. To define the contributions of individual Fzd proteins to osteoblast function, we profiled the express...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29563

    authors: Kushwaha P,Kim S,Foxa GE,Michalski MN,Williams BO,Tomlinson RE,Riddle RC

    更新日期:2020-10-01 00:00:00

  • Expression of transforming growth factor-beta receptor type II and tumorigenicity in human breast adenocarcinoma MCF-7 cells.

    abstract::To analyze transforming growth factor-beta (TGF-beta) response during MCF-7 cell progression, early passage (MCF-7E, < 200 passage) and late passage (MCF-7L, > 500 passage) cells were compared. MCF-7E cells showed an IC50 of approximately 10 ng/ml of TGF-beta1, whereas MCF-7L cells were insensitive. MCF-7E cells conta...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199808)176:2<424::AID-JCP2

    authors: Ko Y,Banerji SS,Liu Y,Li W,Liang J,Soule HD,Pauley RJ,Willson JK,Zborowska E,Brattain MG

    更新日期:1998-08-01 00:00:00

  • Enhanced islet expansion by beta-cell proliferation in young diabetes-prone rats fed a protective diet.

    abstract::Type 1 diabetes is inhibited in diabetes-prone BioBreeding (BBdp) rats fed a low-antigen hydrolyzed casein (HC) diet. In cereal-fed BBdp rats, islet expansion is defective accompanied by a futile upregulation of islet neogenesis without increased islet mass, due to a subtle blockage in islet cell cycle. We hypothesize...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22151

    authors: Wang GS,Kauri LM,Patrick C,Bareggi M,Rosenberg L,Scott FW

    更新日期:2010-08-01 00:00:00

  • Interrelationships between water and cellular metabolism in Artemia cysts. IV. Adenosine 5'-triphosphate and cyst hydration.

    abstract::The concentration of ATP in cysts of the brine shrimp, Artemia salina, has been studied as a function of cyst hydration. Cysts dried over CaSO4 contain 0.02 gH2O/g cysts and 0.54 +/- 0.05 (S.D.) mumoles of ATP/g dried cysts. Addition of water up to 0.05 g/g cysts produced no net change in the level of ATP during incub...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040880205

    authors: Clegg JS,Cavagnaro J

    更新日期:1976-06-01 00:00:00

  • Phenotypic changes of adult porcine mesenchymal stem cells induced by prolonged passaging in culture.

    abstract::The in vitro culture of porcine bone marrow-derived mesenchymal stem cells (MSCs) was used for the investigation of adult stem cell biology. Isolated porcine MSCs possessed the ability to proliferate extensively in an antioxidants-rich medium containing 5% fetal bovine serum (FBS). Greater than 40 serial MSC passages ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20376

    authors: Vacanti V,Kong E,Suzuki G,Sato K,Canty JM,Lee T

    更新日期:2005-11-01 00:00:00

  • Acyl-CoA synthetase short-chain family member 2 (ACSS2) is regulated by SREBP-1 and plays a role in fatty acid synthesis in caprine mammary epithelial cells.

    abstract::Sterol regulatory element binding protein 1 (SREBP-1) is well-known as the master regulator of lipogenesis in rodents. Acyl-CoA synthetase short-chain family member 2 (ACSS2) plays a key role in lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis. ATP citrate lyase (ACLY) catalyzes the conversion of ci...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25954

    authors: Xu H,Luo J,Ma G,Zhang X,Yao D,Li M,Loor JJ

    更新日期:2018-02-01 00:00:00

  • Protein tyrosine phosphorylation and calcium signaling in thyroid FRTL-5 cells.

    abstract::We examined the importance of tyrosine kinase(s) on the ATP-evoked Ca2+ entry and DNA synthesis of thyroid FRTL-5 cells. ATP rapidly and transiently tyrosine phosphorylated a 72-kDa protein(s). This phosphorylation was abolished by pertussis toxin and by the tyrosine kinase inhibitor genistein, and was dependent on Ca...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199805)175:2<211::AID-JCP1

    authors: Törnquist K,Dugué B,Ekokoski E

    更新日期:1998-05-01 00:00:00

  • miR-335-5p induces insulin resistance and pancreatic islet β-cell secretion in gestational diabetes mellitus mice through VASH1-mediated TGF-β signaling pathway.

    abstract::Multiple studies have reported different methods in treating gestational diabetes mellitus (GDM); however, the relationship between miR-335-5p and GDM still remains unclear. Here, this study explores the effect of miR-335-5p on insulin resistance and pancreatic islet β-cell secretion via activation of the TGFβ signali...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27406

    authors: Tang XW,Qin QX

    更新日期:2019-05-01 00:00:00

  • Agonist-induced calcium entry correlates with STIM1 translocation.

    abstract::The mechanisms of agonist-induced calcium entry (ACE) following depletion of intracellular calcium stores have not been fully established. We report here that calcium-independent phospholipase A (iPLA(2)) is required for robust Ca(2+) entry in HaCaT keratinocytes following ATP or UTP stimulation. Lysophosphatidic acid...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20993

    authors: Ross K,Whitaker M,Reynolds NJ

    更新日期:2007-06-01 00:00:00

  • Regulation of sugar transport in cultured diploid human skin fibroblasts.

    abstract::The regulation of hexose transport under glucose-starvation conditions was studied in cultured human skin fibroblasts. Glucose starvation enhanced the transport of 2-DG and 3-0-methyl-D-glucose (3-OMG) but not of L-glucose. Glucose-starvation enhanced transport was inhibited by cytochalasin B (10 microM). The starvati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041120309

    authors: Germinario RJ,Rockman H,Oliveira M,Manuel S,Taylor M

    更新日期:1982-09-01 00:00:00

  • Transient receptor potential vanilloid 1 activation induces inflammatory cytokine release in corneal epithelium through MAPK signaling.

    abstract::In certain epithelial tissues, activation of transient receptor potential (TRP) vanilloid subtype 1 (TRPV1) by noxious stimuli induces pro-inflammatory cytokine release, which helps to mitigate the challenge. While the corneal epithelium elicits such responses to a variety of challenges, it remains unknown whether TRP...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21141

    authors: Zhang F,Yang H,Wang Z,Mergler S,Liu H,Kawakita T,Tachado SD,Pan Z,Capó-Aponte JE,Pleyer U,Koziel H,Kao WW,Reinach PS

    更新日期:2007-12-01 00:00:00

  • Insulin stimulus-secretion coupling is triggered by a novel thiazolidinedione/sulfonylurea hybrid in rat pancreatic islets.

    abstract::New compounds with promising antidiabetic activity were synthesized. For the first time, a portion of the glibenclamide molecule was bound to a part of the core structure of thiazolidinedione to evaluate insulin secretagogue activity. Following studies in our laboratory, 4-{2-[2-(3,4-dichlorophenyl)-4-oxo-1,3-thiazoli...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26746

    authors: Mendes CP,Postal BG,Oliveira GTC,Castro AJG,Frederico MJS,Moraes ALL,Neuenfeldt PD,Nunes RJ,Menegaz D,Silva FRMB

    更新日期:2018-01-01 00:00:00

  • Selective release from cultured mammalian cells of heat-shock (stress) proteins that resemble glia-axon transfer proteins.

    abstract::Cultured rat embryo cells were stimulated to rapidly release a small group of proteins that included several heat-shock proteins (hsp110, hsp71, hscp73) and nonmuscle actin. The extracellular proteins were analyzed by two-dimensional polyacrylamide gel electrophoresis. Heat-shocked cells released the same set of prote...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041380206

    authors: Hightower LE,Guidon PT Jr

    更新日期:1989-02-01 00:00:00

  • The circ_0021977/miR-10b-5p/P21 and P53 regulatory axis suppresses proliferation, migration, and invasion in colorectal cancer.

    abstract::An in-depth understanding of circular RNAs (circRNAs) indicates that they are abundant in the eukaryotic transcriptome. Many circRNAs act as microRNA sponges; thus, they represent a new type of regulatory factor. However, the role of circRNA in colorectal cancer (CRC) remains largely unknown. Low circ_0021977 expressi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29135

    authors: Lu C,Jiang W,Hui B,Rong D,Fu K,Dong C,Tang W,Cao H

    更新日期:2020-03-01 00:00:00

  • Regulation of myelin basic protein gene transcription: identification of a distal cis-acting regulatory element.

    abstract::The myelin basic protein (MBP) gene contains sequences located upstream of its transcription start site which play a key role in glial-specific transcription of the MBP promoter. Earlier analysis of the 320 bp upstream regulatory sequence of MBP has revealed multiple cis-acting regulatory motifs which differentially r...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041630213

    authors: Haque NS,Haas S,Knobler RL,Khalili K

    更新日期:1995-05-01 00:00:00

  • Bone marrow mesenchymal stem cells modified by angiogenin-1 promotes tissue repair in mice with oxygen-induced retinopathy of prematurity by promoting retinal stem cell proliferation and differentiation.

    abstract::Retinopathy has become one of the major factors that lead to blindness worldwide. Although many clinical therapies are concerned about such disease, most of them focus on symptoms alleviation. In this study, we aim to investigate whether coculture retinal stem cells (RSCs) with bone marrow mesenchymal stem cells trans...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28706

    authors: Ma QQ,Liu FY,Shi M,Sun CH,Tan Z,Chang XD,Li QP,Feng ZC

    更新日期:2019-11-01 00:00:00

  • Production of a colony-stimulating factor following differentiation of leukemic myleoblasts to macrophages.

    abstract::Leukemic cells in the myeloblastic stage from a murine myeloid leukemia cell line (M1) were induced to differentiate to macrophages by lipopolysaccharide (LPS) from Gram-negative bacteria. A granulocyte-macrophage colony-stimulating factor (CSF) was produced only during differentiation. After induction of differentiat...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041020307

    authors: Maeda M,Ichikawa Y

    更新日期:1980-03-01 00:00:00

  • Exosomes are the novel players involved in the beneficial effects of exercise on type 2 diabetes.

    abstract::Exosomes contain regulatory signals such as lipids, proteins, and nucleic acids which can be transferred to adjacent or remote cells to mediate cell-to-cell communication. Exercise is a positive lifestyle for metabolic health and a nonpharmacological treatment of insulin resistance and metabolic diseases. Moreover, ex...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28319

    authors: Li G,Liu H,Ma C,Chen Y,Wang J,Yang Y

    更新日期:2019-02-12 00:00:00

  • Cartilage regeneration with dual-drug-releasing injectable hydrogel/microparticle system: In vitro and in vivo study.

    abstract::In this study, we developed an injectable in situ forming hydrogel/microparticle system consisting of two drugs, melatonin and methylprednisolone, to investigate the capability of the system for chondrogenesis in vitro and in vivo. The chemical, mechanical, and rheological properties of the hydrogel/microparticle were...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.30006

    authors: Naghizadeh Z,Karkhaneh A,Nokhbatolfoghahaei H,Farzad-Mohajeri S,Rezai-Rad M,Dehghan MM,Aminishakib P,Khojasteh A

    更新日期:2021-03-01 00:00:00

  • Distinct characteristics of heregulin signals mediated by HER3 or HER4.

    abstract::Members of the epidermal growth-factor-receptor tyrosine-kinase (EGFR) family play important roles both in normal growth regulation/cell differentiation and in the genesis and progression of human neoplasia. In the present study, we analysed distinct heregulin (HRG) signals mediated by the HRG receptors HER3 and HER4....

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199711)173:2<187::AID-JCP1

    authors: Weiss FU,Wallasch C,Campiglio M,Issing W,Ullrich A

    更新日期:1997-11-01 00:00:00

  • Modulation of transforming growth factor-beta 1 stimulated elastin and collagen production and proliferation in porcine vascular smooth muscle cells and skin fibroblasts by basic fibroblast growth factor, transforming growth factor-alpha, and insulin-like

    abstract::During tissue repair and development, matrix accumulation is modulated as multiple signals impinge on target cells. We have investigated the effects of combinations of the mitogenic cytokines, basic fibroblast growth factor (bFGF), transforming growth factor alpha (TGF-alpha), and insulin-like growth factor-1 (IGF-1) ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041550119

    authors: Davidson JM,Zoia O,Liu JM

    更新日期:1993-04-01 00:00:00

  • MiR-145 Regulates Lipogenesis in Goat Mammary Cells Via Targeting INSIG1 and Epigenetic Regulation of Lipid-Related Genes.

    abstract::MicroRNAs (miRNAs) are noncoding RNA molecules that regulate gene expression at the post-transcriptional level to cause translational repression or degradation of targets. The profiles of miRNAs across stages of lactation in small ruminant species such as dairy goats is unknown. A small RNA library was constructed usi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25499

    authors: Wang H,Shi H,Luo J,Yi Y,Yao D,Zhang X,Ma G,Loor JJ

    更新日期:2017-05-01 00:00:00

  • Role of RB and RB2/P130 genes in marrow stromal stem cells plasticity.

    abstract::Marrow stromal cells (MSCs) are stem-like cells having a striking somatic plasticity. In fact, besides differentiating into mesenchymal lineages (bone, cartilage, and fat), they are capable of differentiating into neurons and astrocytes in vitro and in vivo. The RB and RB2/P130 genes, belonging to the retinoblastoma g...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20026

    authors: Jori FP,Napolitano MA,Melone MA,Cipollaro M,Cascino A,Giordano A,Galderisi U

    更新日期:2004-08-01 00:00:00

  • S100A1 and S100B: Calcium Sensors at the Cross-Roads of Multiple Chondrogenic Pathways.

    abstract::The S100 protein family comprises more than 20 members of small calcium binding proteins operating as Ca2 +-activated switches that interact and modulate the activity of a large number of targets. S100A1 and S100B, two members of this family, have been recently associated with the differentiation status of human artic...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.25720

    authors: Diaz-Romero J,Nesic D

    更新日期:2017-08-01 00:00:00

  • Survivin modulatory role in autoimmune and autoinflammatory diseases.

    abstract::Baculoviral IAP repeat containing 5 (BIRC5) gene encodes the important protein as survivin, a multifunctional protein, which is involved in cellular and molecular networks, progression of cell cycle, homeostasis, developmental morphogenesis, and apoptosis. The proximal BIRC5 promoter possesses specific binding sites f...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28725

    authors: Pahlavan Y,Kahroba H,Samadi N,Karimi A,Ansarin K,Khabbazi A

    更新日期:2019-11-01 00:00:00

  • Expression of mitochondrial transcription factor A (TFAM) during porcine gametogenesis and preimplantation embryo development.

    abstract::Mitochondrial transcription factor A (TFAM) is responsible for stability, maintenance, and transcriptional control of mitochondrial DNA (mtDNA). We have studied the expression and distribution of TFAM in the gametes and preimplantation embryos of the domestic pig (Sus scrofa). We hypothesized that TFAM is not present ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21528

    authors: Antelman J,Manandhar G,Yi YJ,Li R,Whitworth KM,Sutovsky M,Agca C,Prather RS,Sutovsky P

    更新日期:2008-11-01 00:00:00