Abstract:
:Heparan sulfate (HS) is a polysaccharide known to modulate many important biological processes, including Wnt signaling. However, the biochemical interaction between HS and Wnt molecules is not well characterized largely due to the lack of suitable methods. To determine the Wnt binding domain in HS, we used a Wnt signaling-inhibitory antibody (HS20) and a panel of synthetic HS oligosaccharides with distinct lengths and sulfation modifications. We found that the binding of HS20 to heparan sulfate required sulfation at both the C2 position (2-O-sulfation) and C6 position (6-O-sulfation). The oligosaccharides with the greatest competitive effect for HS20 binding were between six and eight saccharide residues in length. Additionally, a four residue-long oligosaccharide could also be recognized by HS20 if an additional 3-O-sulfation modification was present. Furthermore, similar oligosaccharides with 2-O, 6-O and 3-O-sulfations showed inhibition for Wnt activation. These results have revealed that HS20 and Wnt recognize a HS structure containing IdoA2S and GlcNS6S, and that the 3-O-sulfation in GlcNS6S3S significantly enhances the binding of both HS20 and Wnt. This study provides the evidence for identifying the Wnt binding domain in HS and suggests a therapeutic approach to target the interaction of Wnt and HS in cancer and other diseases.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Gao W,Xu Y,Liu J,Ho Mdoi
10.1038/srep26245subject
Has Abstractpub_date
2016-05-17 00:00:00pages
26245issn
2045-2322pii
srep26245journal_volume
6pub_type
杂志文章abstract::In this paper, we propose a novel bio-compatible organic humidity sensor based on natural inner egg shell membrane (IESM) with multilayer cross linked fiber structure that can be used as a substrate as well as a sensing active layer. To fabricate the proposed sensors, two different size inter digital electrodes (IDEs)...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42337-0
更新日期:2019-04-09 00:00:00
abstract::Realization of phosphor-free white-light emitters is becoming an important milestone on the road to achieve high quality and reliability in high-power white-light-emitting diodes (LEDs). However, most of reported methods have not been applied to practical use because of their difficulties and complexity. In this study...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17372
更新日期:2015-12-02 00:00:00
abstract::Anemia is commonly seen in preterm infants. It may reduce the capacity of hemoglobin to transport oxygen throughout the body and may result in tissue and organ dysfunction. This study aimed to investigate the effect of anemia on the development of bronchopulmonary dysplasia (BPD) in preterm infants. 243 infants who we...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/srep22717
更新日期:2016-03-03 00:00:00
abstract::Backward wave with anti-parallel phase and group velocities is one of the basic properties associated with negative refraction and sub-diffraction image that have attracted considerable interest in the context of photonic metamaterials. It has been predicted theoretically that some plasmonic structures can also suppor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep20448
更新日期:2016-02-04 00:00:00
abstract::Although early diagnosis and treatment reduce the risk of blindness from glaucoma, the decision on whether or not to begin treatment in patients with suspected glaucoma is often a dilemma because the majority of patients never develop definite glaucoma. A growing body of evidences suggests that posterior bowing of the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17843-8
更新日期:2018-01-15 00:00:00
abstract::Hysteresis, which is the history dependence of physical systems, is one of the most important topics in physics. Interestingly, bi-stability of plasma with a huge hysteresis loop has been observed in inductive plasma discharges. Despite long plasma research, how this plasma hysteresis occurs remains an unresolved ques...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15254
更新日期:2015-10-20 00:00:00
abstract::What's on the label is not what's in the bottle, from food products to herbal medicinal products (HMPs), economically-motivated biomaterials adulteration is a long-term problem affecting the food and drug industry. Accurate identification of the biomaterial ingredients in processed commodities is highly desirable. In ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05083-9
更新日期:2017-07-06 00:00:00
abstract::To investigate the safety and efficiency of using robotic staplers for intracorporeal gastroduodenostomy in reduced-port robotic gastrectomy for gastric adenocarcinoma. We retrospectively reviewed patients who underwent totally robotic and laparoscopic gastrectomy with intracorporeal gastroduodenostomy. Gastroduodenos...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71807-z
更新日期:2020-09-07 00:00:00
abstract::The production of male and female offspring is often determined by the presence of specific sex chromosomes which control sex-specific expression, and sex chromosomes evolve through reduced recombination and specialized gene content. Here we present the genomes of Chrysomya rufifacies, a monogenic blow fly (females pr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72880-0
更新日期:2020-09-25 00:00:00
abstract::E5 proteins are amongst the least understood of the Human Papillomavirus (HPV) encoded gene products. They are small, membrane-integrated proteins known to modulate a number of critical host pathways associated with pathogenesis including growth factor receptor signaling and immune evasion. Their role in the virus lif...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep12523
更新日期:2015-08-03 00:00:00
abstract::sp-Hybridized carbon atomic wires are appealing systems with large property tunability. In particular, their electronic properties are intimately related to length, structure, and type of functional end-groups as well as to other effects such as the intermolecular charge transfer with metal nanoparticles. Here, by a c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-38367-9
更新日期:2019-02-07 00:00:00
abstract::The aim of this study was to investigate the characteristics of insulin producing cells (IPCs) differentiated from adipose-tissue derived stem cells (ADSCs) isolated from human subcutaneous and visceral adipose tissues and identify ADSCs suitable for differentiation into efficient and functional IPCs. Subcutaneous and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-49701-0
更新日期:2019-09-13 00:00:00
abstract::Until now, the only kind of practical quantum private query (QPQ), quantum-key-distribution (QKD)-based QPQ, focuses on the retrieval of a single bit. In fact, meaningful message is generally composed of multiple adjacent bits (i.e., a multi-bit block). To obtain a message a1a2···al from database, the user Alice has t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep07537
更新日期:2014-12-18 00:00:00
abstract::Controlling light propagation intentionally through turbid media such as ground glass or biological tissue has been demonstrated for many useful applications. Due to random scattering effect, one of the important goals is to draw a desired shape behind turbid media with a swift and precise method. Feedback wavefront s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56214-3
更新日期:2019-12-20 00:00:00
abstract::A modular p-phosphonated calix[4]arene vesicle (PCV) loaded with paclitaxel (PTX) and conjugated with folic acid as a cancer targeting ligand has been prepared using a thin film-sonication method. It has a pH-responsive capacity to trigger the release of the encapsulated PTX payload under mildly acidic conditions. PTX...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep23489
更新日期:2016-03-24 00:00:00
abstract::The presenilin 1 (PSEN1) L271V mutation causes early-onset familial Alzheimer's disease by disrupting the alternative splicing of the PSEN1 gene, producing some transcripts harboring the L271V point mutation and other transcripts lacking exon 8 (PS1(∆exon8)). We previously reported that PS1 L271V increased amyloid bet...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17042
更新日期:2015-11-26 00:00:00
abstract::In this work, we utilise a combination of theory, computation and experiments to understand the early events related to the nucleation of Ag filaments on α-Ag2WO4 crystals, which is driven by an accelerated electron beam from an electron microscope under high vacuum. The growth process and the chemical composition and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05391
更新日期:2014-06-23 00:00:00
abstract::Ulcerative colitis (UC) is a chronic relapsing-remitting form of inflammatory bowel disease (IBD) that increases the risk of colorectal cancer, the third most common malignancy in humans. Oxidative stress is a risk factor for the development of UC. The Keap1-Nrf2-ARE pathway is one of the most important defensive mech...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26585
更新日期:2016-05-24 00:00:00
abstract::Advanced electron microscopy technologies have made it possible to perform precise double-slit interference experiments. We used a 1.2-MV field emission electron microscope providing coherent electron waves and a direct detection camera system enabling single-electron detections at a sub-second exposure time. We devel...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19380-4
更新日期:2018-01-17 00:00:00
abstract::Circularly polarized laser sources with small footprints and high efficiencies can possess advanced functionalities in optical communication and biophotonic integrated systems. However, the conventional lasers with additional circular-polarization converters are bulky and hardly compatible with nanophotonic circuits, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26578
更新日期:2016-05-25 00:00:00
abstract::The aims of the study were to clarify the impact of storage time and centrifugation strategy on brain-derived neurotrophic factor (BDNF) levels in human serum and plasma samples. In addition, we analyzed associations between BDNF levels, cardiorespiratory fitness and waist circumference. Seventeen healthy males (25.2 ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-45976-5
更新日期:2019-07-04 00:00:00
abstract::Cytometric studies utilizing flow cytometry or multi-well culture plate fluorometry are often limited by a deficit in temporal resolution and a lack of single cell consideration. Unfortunately, many cellular processes, including signaling, motility, and molecular transport, occur transiently over relatively short peri...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05117
更新日期:2014-05-30 00:00:00
abstract::This study aims to determine the prevalence of health personnel with normal weight central obesity and to investigate whether this group had higher cardiovascular disease (CVD) risk factors than those of the people with normal weight and without central obesity. A waist-to-height ratio was calculated as waist circumfe...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/srep37100
更新日期:2016-11-16 00:00:00
abstract::Reef sharks are vulnerable predators experiencing severe population declines mainly due to overexploitation. However, beyond direct exploitation, human activities can produce indirect or sub-lethal effects such as behavioral alterations. Such alterations are well known for terrestrial fauna but poorly documented for m...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37145-x
更新日期:2019-02-27 00:00:00
abstract::The activation of the majority of AGC kinases is regulated by two phosphorylation events on two conserved serine/threonine residues located on the activation loop and on the hydrophobic motif, respectively. In AGC kinase family, phosphomimetic substitutions with aspartate or glutamate, leading to constitutive activati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-56937-3
更新日期:2020-01-17 00:00:00
abstract::Copper complexes with potent anti-tumor effect have been extensively developed. Most investigations of their modes of action focused on the biomolecular targets but not the signal transduction between target binding and cell death. We have previously shown that the cytotoxic complex pyridine(2,4-dihydroxybenzaldehyde ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36868
更新日期:2016-11-14 00:00:00
abstract::East Asian summer monsoon (EASM)-driven rapid hydroclimatic variation is a crucial factor with major socioeconomic impacts. Nevertheless, decadal- to centennial-scale EASM variability over the last two millennia is still poorly understood. Pollen-based quantitative annual precipitation (PqPann) and annual precipitatio...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-74994-x
更新日期:2020-10-20 00:00:00
abstract::Membrane fouling, i.e. accumulation of unwanted material on the surface of the membrane is a significant problem in filtration processes since it commonly degrades membrane performance and increases operating costs. Therefore, the advantages of early stage monitoring and control of fouling are widely recognized. In th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29268-y
更新日期:2018-07-23 00:00:00
abstract::Hydrogels are being extensively used for three-dimensional immobilization and culture of cells in fundamental biological studies, biochemical processes, and clinical treatments. However, it is still a challenge to support viability and regulate phenotypic activities of cells in a structurally stable gel, because the g...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08948
更新日期:2015-03-10 00:00:00
abstract::In this work the chalcopyrite CuIn3Se5-xTex (x = 0~0.5) with space group through isoelectronic substitution of Te for Se have been prepared, and the crystal structure dilation has been observed with increasing Te content. This substitution allows the anion position displacement ∆u = 0.25-u to be zero at x ≈ 0.15. Howe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40224
更新日期:2017-01-06 00:00:00