Abstract:
BACKGROUND:This study aimed to find the linker with minimal impact among chains to fight against the structure and function of cervical cancer (CC) single-chain antibody. MATERIALS AND METHODS:The original variable region of heavy chain (VH) and variable region of light chain (VL), and the single-chain antibody with linkers of different lengths (n = 1-8) were modeling by homologous modeling, while the peptide chain structure of (Gly4Ser)n was utilized by the linkers. Comparison of the similarity of original VH/VL and VHn/VLn was carried out by applying the algorithm of spatial hierarchical alignment based on the spherical coordinates. The fore and aft distance and diffusion radius of alpha (α) were also calculated. The stability of antibody with different linker length was then compared. ELISA method was adopted to evaluate the immunological activity of single-chain antibody with different linkers. MTT assay was used to analyze the inhibition effect of ScFv-n on CC cells. RESULTS:When n = 4, the structures were the most similar between ScFv and the original VH/VL. When n = 3, the influence of adding connecting peptide on the stability of single-chain antibody was the least. The result of ELISA and MTT methods indicated that when n = 3, single-chain antibody gained the highest activity. CONCLUSION:The optimum length of linker of anti-human CC single-chain antibody was n = 3 from the point of mathematical modeling and biology experiments. This study provided new ideas for the design and constructions of single-chain antibody, and theoretical basis for the treatment of CC.
journal_name
Eur J Gynaecol Oncoljournal_title
European journal of gynaecological oncologyauthors
Cheng J,Dul Qx,Zhang,Ren Yf,Zhang Ba,Feng Xdsubject
Has Abstractpub_date
2016-01-01 00:00:00pages
171-7issue
2eissn
0392-2936issn
2709-0086journal_volume
37pub_type
杂志文章abstract::The study population was derived from 482 patients with breast cancer treated at the Department of Oncology, University Hospital Center Zagreb, between 1992 and 1999. The main purpose of our study was to evaluate differences in breast cancer characteristics and treatment in a population of women with breast cancer old...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The elderly population is increasing in number. Aggressive therapeutic intervention in this patient group may not always be possible because of age, the presence of co-morbidity, and poor functional status. Hence, individualized management of cervix cancer (CC) in the elderly is often practiced. Because of the precedi...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::A 47-year-old patient with two previous deliveries and three deliberate abortions was admitted to the Institute of Gynecology and Obstetrics, Clinical Center of Serbia with the diagnosis of a uterine myoma and left adnexal mass. Five years previously, she had undergone excision of a malignant melanoma from her left le...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The true struma ovarii is a rare teratomatous neoplasia, composed with typical thyroid tissue. Generally it is asymptomatic or only determines a mild hypogastric weighting feeling. In a few subjects this neoplasm is secreting and rise to hyperthyroidism. The Authors describe the clinical and anatomopathological charac...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::The Authors, on the basis of the literature and their own experience, propose a diagnostic schedule for detection of breast disease based on integrated thermography, diaphanoscopy, echography and mammography. ...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract:OBJECTIVE:To evaluate p53 protein expression in the endometrial polyp and compare with adenocarcinoma and atrophic endometrium of postmenopausal women. MATERIALS AND METHODS:Ninety-eight postmenopausal women were included in this study and divided into three groups related to histopathologic diagnosis: Group A--endome...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:To further study the clinicopathologic and immunohistochemical features of ovarian granulosa cell tumors (GCTs). METHODS:We retrospectively studied all cases of GCTs diagnosed in our laboratory over the last 10-year period. Immunohistochemistry for inhibin, vimentin, cytokeratin, Ki-67 and p53 was performed on...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::The results obtained by an experimental clinical study about the lymphatic drainage of the uterine cervix by direct intraparenchymal injection of Lipiodol are reported. This study suggests a new approach to the evaluation of cervical carcinoma staging complementary to the peripheral lymphangiography. ...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:Some Japanese institutes have been performing a population screening program for cervix cancer involving the simultaneous use of Pap smear and colposcopy. This program may be a good model for evaluating the efficacy of Pap smears and colposcopy. METHODS & MATERIALS:The subjects included 2,000 women who under...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVE:To report the authors' experience with laparoscopic ovarian transposition and ovarian function preservation in young women with cervical squamous cell carcinoma treated by primary pelvic irradiation. MATERIALS AND METHODS:Twenty-seven premenopausal patients were treated with radiotherapy for a cervical squam...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::According to the present guidelines for advanced epithelial ovarian cancer (EOC), bulky lymph nodes should be removed as part of the routine surgical staging and the primary goal being removal of all macroscopic tumor residuals. Furthermore, EOC-patients with bulky lymph node relapse seem to benefit from lymphadenecto...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract:BACKGROUND:Small cell neuroendocrine carcinoma of the breast is a rare tumor with fewer than 30 cases reported in the literature. The reported age of incidence of mammary small cell carcinoma is similar to that of breast carcinoma of the usual types. CASE:The clinicopathologic findings of a primary mammary small cell ...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章,评审
doi:
更新日期:2007-01-01 00:00:00
abstract::Endometrial adenocarcinoma is the most common malignant neoplasm of the female genital tract and, despite its relative frequency, the molecular events that contribute to the development and progression of the lesion remain poorly understood. The normal human endometrium is characterized by hormone-dependent variations...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章,评审
doi:
更新日期:2005-01-01 00:00:00
abstract::We present a case of well-differentiated papillary mesothelioma discovered during staging surgery for endometrial carcinoma in a 50-year-old postmenopausal woman. In case of simultaneous well-differentiated papillary mesothelioma (WDPM) and endometrial carcinoma, the surgeon may be mistaken by considering peritoneal i...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::During the 2000-2007 period, my group and I reviewed the cases of 3,036 patients undergoing routine colposcopies, cytology and cervical biopsies for those cases that merited it, and a 20% increase was found in lesions defined as dysplasias as compared with those found in the studies of the previous century. This is so...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract:PURPOSE OF INVESTIGATION:To determine the positive predictive value (PPV) of both preoperative radiological and intraoperative identification of splenic disease in cases of advanced and recurrent gynaecological malignancy. MATERIALS AND METHODS:A retrospective study of all splenectomies performed during surgeries for ...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2016-01-01 00:00:00
abstract::For both cervical cancer (UCC) and endometrial cancer (EMC) there are no effective prognostic markers. In this study, we evaluated HIG2 protein expression in 332 uterine cancers (186 UCCs and 146 EMCs) and examined the relationship between HIG2 protein expression and clinical factors, including prognosis. Totally, HIG...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to examine the prognostic significance of vascular endothelial growth factor (VEGF) in epithelial ovarian cancer (EOC). METHODS:Surgical specimens of 105 patients with primary EOC FIGO Stages 1 to 4, who underwent surgical staging, were investigated. Expression of VEGF was evaluate...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract:OBJECTIVE:The objective of this study is to describe a new technique of laparoscopic radical hysterectomy without vaginal cuff closure. METHODS:Three patients underwent laparoscopic radical hysterectomy, bilateral salpingo-oophorectomy and bilateral pelvic lymph node dissection using an Argon Beam Coagulator. Four tro...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:OBJECTIVE:In order to determine the significance of proliferative activity (PA) in endometrial carcinomas, we analysed the expression of cell cycle-related antigens in routinely processed tissue. MATERIALS AND METHODS:Serial sections of 113 endometrial carcinoma specimens were immunostained with the monoclonal antibod...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Extramedullary hematopoiesis (EH) is the ectopic production of myeloid, erythroid and megakaryocytic elements. In postfetal life it usually occurs in conditions with hyperactive, depleted or infiltrated marrow; it is extremely rare in the genital tract. We report a case of EH in the uterine isthmus (UI) which was foun...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章,评审
doi:
更新日期:2002-01-01 00:00:00
abstract::Exaggerated placental site is defined as a non-neoplastic trophoblastic lesion featuring exuberant infiltration into the endometrium and myometrium by intermediate trophoblasts and syncytiotrophoblasts. Exaggerated placental site can occur following normal or ectopic pregnancy, abortion, or hydatidiform mole. We encou...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章,评审
doi:
更新日期:2010-01-01 00:00:00
abstract::Pelvic lipomatosis consists of an abdominal capsulated mass containing lipidic tissue, generally with remarkable dimensions, responsible for urinary tract disturbances. Here we describe the first case to our knowledge of accidental intraoperative diagnosis associated to an ovarian cyst in absence of objective symptoms...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Forty-seven women affected by Stage Ic-IV epithelial carcinoma of the ovary were treated with the combination of cisplatin, adriamycin (40-50 mg/m2 day 1), and cyclophosphamide (800 mg/m2 day 1) (CAP). Two different schedules of cisplatin were used: 20 mg/m2 day 1-->5 (CAP 5), or 80 mg/m2 on day 1 (CAP 1). In the grou...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract:OBJECTIVE:This study aims to investigate the protective and sensitive effects of melatonin (MLT) in the treatment of breast cancer. MATERIALS AND METHODS:ER+ breast cancer rat model was established and then rats were randomly divided into five different groups as follows: control group, Diss group, adriamycin (ADM) gr...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::It is difficult to find effective chemotherapy combination as third line treatment in epithelial ovarian cancer. In this paper the therapeutic response was evaluated of weekly administration of cis-platinum as third line chemotherapy, for a minimum of 6 cycles, on 8 patients, aged 41-72 years, with advanced ovarian ca...
journal_title:European journal of gynaecological oncology
pub_type: 临床试验,杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::The term female adnexal tumor of probable Wolffian origin "FATWO" designs this tumor wich arises by the rare persisting remnants of the mesonephric duct (Wolffian duct). About 40 cases have been reported in literature. Few cases of recurrence have been reported, FATWO usually shows no signs of hormonal activity. We re...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Malignant ovarian tumors have been continuously increasing in Western countries and represent the leading cause of death for gynecological cancer. In fact, the mortality for malignant ovarian tumors remains very high with a low percentage of 5-year survival in the advanced stage of disease. The aim of this study was t...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:PURPOSE OF INVESTIGATION:To investigate the characteristics of patients with recurrent cervical carcinoma after radical hysterectomy and pelvic lymph node dissection (RHND), and to evaluate the effect of clinical and surgical pathologic factors on the outcome of these patients. METHODS:Data from the files of 32 patien...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The purpose of our study was to examine the concordance among colposcopy, cytology, and histology in the diagnoses of intraepithelial lesions of the uterine cervix. We compared the results of Pap tests, biopsies, and colposcopy in 190 patients, who had histologically proven CIN and/or HPV infection, using histology as...
journal_title:European journal of gynaecological oncology
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00