Abstract:
:Dietary nitrate is now recognized as an alternative substrate for nitric oxide (•NO) production in the gut. This novel pathway implies the sequential reduction of nitrate to nitrite, •NO and other bioactive nitrogen oxides but the physiological relevance of these oxidants has remained elusive. We have previously shown that dietary nitrite fuels an hitherto unrecognized nitrating pathway at acidic gastric pH, through which pepsinogen is nitrated in the gastric mucosa, yielding a less active form of pepsin in vitro. Here, we demonstrate that pepsin is nitrated in vivo and explore the functional impact of protein nitration by means of peptic ulcer development. Upon administration of pentagastrin and human nitrite-rich saliva or sodium nitrite to rats, nitrated pepsin was detected in the animal's stomach by immunoprecipitation. •NO was measured in the gastric headspace before and after nitrite instillation by chemiluminescence. At the end of each procedure, the stomach's lesions, ranging from gastric erosions to haemorrhagic ulcers, were scored. Nitrite increased gastric •NO by 200-fold (p<0.05) and nitrated pepsin was detected both in the gastric juice and the mucosa (p<0.05). Exogenous urate, a scavenger of nitrogen dioxide radical, blunted •NO detection and inhibited pepsin nitration, suggesting an underlining free radical-dependent mechanism for nitration. Functionally, pepsin nitration prevented the development of gastric ulcers, as the lesions were only apparent when pepsin nitration was inhibited by urate. In sum, this work unravels a novel dietary-dependent nitrating pathway in which pepsin is nitrated and inactivated in the stomach, preventing the progression of gastric ulcers.
journal_name
Redox Bioljournal_title
Redox biologyauthors
Rocha BS,Lundberg JO,Radi R,Laranjinha Jdoi
10.1016/j.redox.2016.04.002subject
Has Abstractpub_date
2016-08-01 00:00:00pages
407-14issn
2213-2317pii
S2213-2317(16)30027-1journal_volume
8pub_type
杂志文章相关文献
Redox Biology文献大全abstract::Strenuous exercise is a potent stimulus to induce beneficial skeletal muscle adaptations, ranging from increased endurance due to mitochondrial biogenesis and angiogenesis, to increased strength from hypertrophy. While exercise is necessary to trigger and stimulate muscle adaptations, the post-exercise recovery period...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2020.101480
更新日期:2020-08-01 00:00:00
abstract::The traditional Asian diet is rich in fruits, vegetables and soy, the latter representing a significant source of dietary isoflavones. The isoflavone prunetin was recently identified to improve intestinal epithelial barrier function in vitro and to ameliorate general survival and overall health state in vivo in male D...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2016.01.001
更新日期:2016-08-01 00:00:00
abstract::The massive production and activation of myofibroblasts (MFB) is key to the development of liver fibrosis. In many studies, it has been proven that hepatocytes are an important part of MFB, and can be transformed into MFB through epithelial-mesenchymal transition (EMT) during hepatic fibrogenesis. In our previous stud...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101600
更新日期:2020-09-01 00:00:00
abstract::Electrophiles and reactive oxygen species (ROS) play a major role in modulating cellular defense mechanisms as well as physiological functions, and intracellular signaling. However, excessive ROS generation (endogenous and exogenous) can create a state of redox imbalance leading to cellular and tissue damage (Ma and H...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2018.11.017
更新日期:2019-02-01 00:00:00
abstract::A reliable and fast liquid chromatography-tandem mass spectrometry method has been developed for the simultaneous determination of three oxidized nucleic acid damage products in urine, 8-oxoguanine (8-oxoGua), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) and 8-oxo-7,8-dihydroguanosine (8-oxoGuo). We applied this me...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2018.11.016
更新日期:2019-01-01 00:00:00
abstract::Activity-induced nitric oxide (NO) imbalance and "nitrosative stress" are proposed mechanisms of disrupted Ca(2+) homeostasis in atrophic skeletal muscle. We thus mapped S-nitrosylated (SNO) functional muscle proteins in healthy male subjects in a long-term bed rest study (BBR2-2 Study) without and with exercise as co...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2013.10.006
更新日期:2013-10-28 00:00:00
abstract::Manganese superoxide dismutase, encoded by the Sod2 gene, is a ubiquitously expressed mitochondrial antioxidant enzyme that is essential for mammalian life. Mice born with constitutive genetic knockout of Sod2 do not survive the neonatal stage, which renders the longitudinal study of the biochemical and metabolic effe...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2013.01.001
更新日期:2013-02-05 00:00:00
abstract::Age-related macular degeneration (AMD) is the leading cause of vision loss in the western world. Recent evidence suggests that RPE and photoreceptors have an interconnected metabolism and that mitochondrial damage in RPE is a trigger for degeneration in both RPE and photoreceptors in AMD. To test this hypothesis, this...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101201
更新日期:2019-06-01 00:00:00
abstract::Hypoxia, a common element in the tumor environment, leads to Hypoxia-Inducible Factor-1α (HIF-1α) stabilization to modulate cellular metabolism as an adaptive response. In a previous study, we showed that inhibition of the nuclear factor erythroid 2-like-2 (NFE2L2; NRF2), a master regulator of many genes coping with e...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101210
更新日期:2019-06-01 00:00:00
abstract::Reactive oxygen and nitrogen species (e.g. H2O2, nitric oxide) confer redox regulation of essential cellular signaling pathways such as cell differentiation, proliferation, migration and apoptosis. In addition, classical regulation of gene expression or activity, including gene transcription to RNA followed by transla...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2015.05.008
更新日期:2015-08-01 00:00:00
abstract::Selenium-binding protein 1 (SELENBP1) has recently been reported to catalyse the oxidation of methanethiol, an organosulfur compound produced by gut microbiota. Two of the reaction products of methanethiol oxidation, hydrogen peroxide and hydrogen sulphide, serve as signalling molecules for cell differentiation. Indee...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2018.11.004
更新日期:2019-01-01 00:00:00
abstract::The nuclear protein HMGB1 (high mobility group box 1) is secreted by monocytes-macrophages in response to inflammatory stimuli and serves as a danger-associated molecular pattern. Acetylation and phosphorylation of HMGB1 are implicated in the regulation of its nucleocytoplasmic translocation for secretion, although in...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101203
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:Macrophages regulate the inflammatory response and affect re-endothelialization. Inflammation and macrophages play important roles in promoting tissue repair, but p38α mitogen-activated protein kinase's role in re-endothelialization is unknown. METHODS AND RESULTS:Wire injuries of carotid arteries and Evans...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101775
更新日期:2021-01-01 00:00:00
abstract::We have previously demonstrated that DGLA treatment along with Delta-5-Desaturase (D5D) siRNA in various types of cancer cells enhances the formation of 8-HOA from COX-2-catalyzed DGLA peroxidation, which in turn inhibits cancer cell growth and migration. However, delivery of naked siRNA remains a formidable challenge...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2018.101085
更新日期:2019-02-01 00:00:00
abstract::Increased permeability of the intestinal barrier is proposed as an underlying factor for obesity-associated pathologies. Consumption of high fat diets (HFD) is associated with increased intestinal permeabilization and increased paracellular transport of endotoxins which can promote steatosis and insulin resistance. Th...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2017.11.002
更新日期:2018-04-01 00:00:00
abstract::Ultraviolet light is the dominant environmental oxidative skin stressor and a major skin aging factor. We studied which oxidized phospholipid (OxPL) mediators would be generated in primary human keratinocytes (KC) upon exposure to ultraviolet A light (UVA) and investigated the contribution of OxPL to UVA responses. Ma...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2018.11.006
更新日期:2019-01-01 00:00:00
abstract::The aim of the present study was to define the role of Trx and Grx on metabolic thiol redox regulation and identify their protein and metabolite targets. The hepatocarcinoma-derived HepG2 cell line under both normal and oxidative/nitrosative conditions by overexpression of NO synthase (NOS3) was used as experimental m...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2018.11.007
更新日期:2019-02-01 00:00:00
abstract::Of the various transcription factors that play a role in controlling oxidative stress, the role of FoxO proteins in skin aging has recently become of interest. Unlike other FoxOs, FoxO6 remains in the nucleus due to the lack of nuclear export signal, so that it may respond sensitively to intracellular stimuli for the ...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101624
更新日期:2020-09-01 00:00:00
abstract::Muscle mass and strength are very important for exercise performance. Training-induced musculoskeletal injuries usually require periods of complete immobilization to prevent any muscle contraction of the affected muscle groups. Disuse muscle wasting will likely affect every sport practitioner in his or her lifetime. E...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2020.101531
更新日期:2020-08-01 00:00:00
abstract::Oxidative stress resulting from the accumulation of high levels of reactive oxygen species is a salient feature of, and a well-recognised pathological factor for, diverse pathologies. One common mechanism for oxidative stress damage is via the disruption of intracellular ion homeostasis to induce cell death. TRPM2 is ...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2020.101755
更新日期:2020-10-01 00:00:00
abstract::Premature senescence, a death escaping pathway for cells experiencing stress, is conducive to aging and cardiovascular diseases. The molecular switch between senescent and apoptotic fate remains, however, poorly recognized. Nrf2 is an important transcription factor orchestrating adaptive response to cellular stress. H...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101304
更新日期:2020-01-01 00:00:00
abstract::Ferroptosis is an outcome of metabolic disorders and closely linked to liver cancer. However, the mechanism underlying the fine regulation of ferroptosis in liver cancer remains unclear. Here, we have identified two categories of genes: ferroptosis up-regulated factors (FUF) and ferroptosis down-regulated factors (FDF...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101211
更新日期:2019-06-01 00:00:00
abstract::Compared to other organs, the brain is especially exposed to oxidative stress. In general, brains from young females tend to present lower oxidative damage in comparison to their male counterparts. This has been attributed to higher antioxidant defenses and a better mitochondrial function in females, which has been li...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2020.101505
更新日期:2020-04-01 00:00:00
abstract::Metabolic control of cellular function is significant in the context of inflammation-induced metabolic dysregulation in immune cells. Generation of reactive oxygen species (ROS) such as hydrogen peroxide and superoxide are one of the critical events that modulate the immune response in neutrophils. When activated, neu...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2016.06.003
更新日期:2016-10-01 00:00:00
abstract::Autophagy is an important cell recycling program responsible for the clearance of damaged or long-lived proteins and organelles. Pharmacological modulators of this pathway have been extensively utilized in a wide range of basic research and pre-clinical studies. Bafilomycin A1 and chloroquine are commonly used compoun...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2016.11.004
更新日期:2017-04-01 00:00:00
abstract::The inducible form of Heme Oxygenase-1 (HO-1), a major endoplasmic reticulum (ER) associated heme protein, is known to play important roles in protection against oxidative and chemical stress by degrading free heme released from degradation of heme proteins. In this study we show that induced expression of HO-1 by sub...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2013.07.004
更新日期:2013-07-23 00:00:00
abstract:OBJECTIVE:Cellular repressor of E1A-stimulated genes (CREG), a vasculoprotective molecule, is significantly downregulated in atherosclerotic vessels through unclear mechanisms. While epigenetic regulation is involved in atherosclerosis development, it is not known if the CREG gene is epigenetically regulated. The aim o...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2020.101444
更新日期:2020-05-01 00:00:00
abstract::FUN14 domain-containing protein 1 (Fundc1)-dependent mitophagy, mainly activated by ischemic/hypoxic preconditioning, benefits acute myocardial reperfusion injury and chronic metabolic syndrome via sustaining mitochondrial homeostasis. Mitochondrial fission plays a pathogenic role in ischemic acute kidney injury (AKI)...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101415
更新日期:2020-02-01 00:00:00
abstract::Emerging evidence suggests that oroxylin A exhibits antitumor effects by inducing cell apoptosis. However, the involved molecular mechanisms have not been elucidated. Here we report that the apoptosis induced by oroxylin A was dependent on p62-mediated activation of caspase-8 in hepatocellular carcinoma cells. Further...
journal_title:Redox biology
pub_type: 杂志文章
doi:10.1016/j.redox.2019.101392
更新日期:2020-01-01 00:00:00
abstract::Mitochondria are principal regulators of cellular function and metabolism through production of ATP for energy homeostasis, maintenance of calcium homeostasis, regulation of apoptosis and fatty acid oxidation to provide acetyl CoA for fueling the electron transport chain. In addition, mitochondria play a key role in c...
journal_title:Redox biology
pub_type: 杂志文章,评审
doi:10.1016/j.redox.2014.07.005
更新日期:2014-07-27 00:00:00