当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > DNA hypomethylation upregulates expression of the MGAT3 gene in HepG2 cells and leads to changes in N-glycosylation of secreted glycoproteins.

DNA hypomethylation upregulates expression of the MGAT3 gene in HepG2 cells and leads to changes in N-glycosylation of secreted glycoproteins.

Abstract：

:Changes in N-glycosylation of plasma proteins are observed in many types of cancer, nevertheless, few studies suggest the exact mechanism involved in aberrant protein glycosylation. Here we studied the impact of DNA methylation on the N-glycome in the secretome of the HepG2 cell line derived from hepatocellular carcinoma (HCC). Since the majority of plasma glycoproteins originate from the liver, the HepG2 cells represent a good model for glycosylation changes in HCC that are detectable in blood, which is an easily accessible analytic material in a clinical setting. Two different concentrations of 5-aza-2'-deoxycytidine (5-aza-2dC) differentially affected global genome methylation and induced different glycan changes. Around twenty percent of 84 glyco-genes analysed changed expression level after the 5-aza-2dC treatment as a result of global genome hypomethylation. A correlation study between the changes in glyco-gene expression and the HepG2 glycosylation profile suggests that the MGAT3 gene might be responsible for the glycan changes consistently induced by both doses of 5-aza-2dC. Core-fucosylated tetra-antennary structures were decreased in quantity likely as a result of hypomethylated MGAT3 gene promoter followed by increased expression of this gene.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Klasić M,Krištić J,Korać P,Horvat T,Markulin D,Vojta A,Reiding KR,Wuhrer M,Lauc G,Zoldoš V

doi

10.1038/srep24363

subject

Has Abstract

pub_date

2016-04-13 00:00:00

pages

24363

issn

2045-2322

pii

srep24363

journal_volume

6

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Development and Validation of Prediction Model for Risk Reduction of Metabolic Syndrome by Body Weight Control: A Prospective Population-based Study.

    abstract：:Several studies have reported that weight control is of paramount importance in reducing the risk of metabolic syndrome. Nevertheless, this well-known association does not provide any practical information on how much weight loss in a given period would reduce the risk of metabolic syndrome in individuals in a persona...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-67238-5

    authors： Lee S,Lee H,Choi JR,Koh SB

    更新日期：2020-06-19 00:00:00

  • Organism-like formation of Schistosoma hemozoin and its function suggest a mechanism for anti-malarial action of artemisinin.

    abstract：:The current theories of antimalarial mechanism of artemisinin are inadequate to fully explain the observed effects. In our study, "organism-like" formation of Schistosoma hemozoin granules by attaching to and utilizing erythrocytes to form new ones was observed. This indicates that heme iron is transferred from erythr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep34463

    authors： Sun J,Li C,Wang S

    更新日期：2016-10-03 00:00:00

  • Heritability estimates of the novel trait 'suppressed in ovo virus infection' in honey bees (Apis mellifera).

    abstract：:Honey bees are under pressure due to abnormal high colony death rates, especially during the winter. The infestation by the Varroa destructor mite and the viruses that this ectoparasite transmits are generally considered as the bees' most important biological threats. Almost all efforts to remedy this dual infection h...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-71388-x

    authors： de Graaf DC,Laget D,De Smet L,Claeys Boúúaert D,Brunain M,Veerkamp RF,Brascamp EW

    更新日期：2020-08-31 00:00:00

  • In vivo estrogenicity of p-phenoxyphenol and p-pentyloxyphenol.

    abstract：:p-Alkoxyphenols (AOPs) are a class of ethers that are widely used in industrial and agricultural productions and daily necessities. p-Phenoxyphenol (PhOP) and p-pentyloxyphenol (PeOP) belong to this class and have been reported to be estrogenic in vitro. However, their in vivo estrogenic activities have rarely been of...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-73271-1

    authors： Wang Y,Xiao H,Yang L,Jia X,Guo X,Zhang Z

    更新日期：2020-10-14 00:00:00

  • Microfluidic Electroporation Coupling Pulses of Nanoseconds and Milliseconds to Facilitate Rapid Uptake and Enhanced Expression of DNA in Cell Therapy.

    abstract：:Standard electroporation with pulses in milliseconds has been used as an effective tool to deliver drugs or genetic probes into cells, while irreversible electroporation with nanosecond pulses is explored to alter intracellular activities for pulse-induced apoptosis. A combination treatment, long nanosecond pulses fol...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-63172-8

    authors： Chang AY,Liu X,Tian H,Hua L,Yang Z,Wang S

    更新日期：2020-04-08 00:00:00

  • Comparative analysis of the genomes of Stylophora pistillata and Acropora digitifera provides evidence for extensive differences between species of corals.

    abstract：:Stony corals form the foundation of coral reef ecosystems. Their phylogeny is characterized by a deep evolutionary divergence that separates corals into a robust and complex clade dating back to at least 245 mya. However, the genomic consequences and clade-specific evolution remain unexplored. In this study we have pr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-17484-x

    authors： Voolstra CR,Li Y,Liew YJ,Baumgarten S,Zoccola D,Flot JF,Tambutté S,Allemand D,Aranda M

    更新日期：2017-12-14 00:00:00

  • Comparing feedforward and recurrent neural network architectures with human behavior in artificial grammar learning.

    abstract：:In recent years artificial neural networks achieved performance close to or better than humans in several domains: tasks that were previously human prerogatives, such as language processing, have witnessed remarkable improvements in state of the art models. One advantage of this technological boost is to facilitate co...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-79127-y

    authors： Alamia A,Gauducheau V,Paisios D,VanRullen R

    更新日期：2020-12-17 00:00:00

  • Probing tissue microstructure by diffusion skewness tensor imaging.

    abstract：:Probing the cellular structure of in vivo biological tissue is a fundamental problem in biomedical imaging and medical science. This work introduces an approach for analyzing diffusion magnetic resonance imaging data acquired by the novel tensor-valued encoding technique for characterizing tissue microstructure. Our a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-79748-3

    authors： Ning L,Szczepankiewicz F,Nilsson M,Rathi Y,Westin CF

    更新日期：2021-01-08 00:00:00

  • Histological and transcriptome analyses of testes from Duroc and Meishan boars.

    abstract：:Meishan boars are known for their early sexual maturity. However, they exhibit a significantly smaller testicular size and a reduced proportion of Sertoli cells and daily sperm production compared with Duroc boars. The testes of Duroc and Meishan boars at 20, 75 and 270 days of age were used for histological and trans...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep20758

    authors： Ding H,Luo Y,Liu M,Huang J,Xu D

    更新日期：2016-02-11 00:00:00

  • Dynamic representation of time in brain states.

    abstract：:The ability to process time on the scale of milliseconds and seconds is essential for behaviour. A growing number of studies have started to focus on brain dynamics as a mechanism for temporal encoding. Although there is growing evidence in favour of this view from computational and in vitro studies, there is still a ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep46053

    authors： Bueno FD,Morita VC,de Camargo RY,Reyes MB,Caetano MS,Cravo AM

    更新日期：2017-04-10 00:00:00

  • Intein-mediated backbone cyclization of VP1 protein enhanced protection of CVB3-induced viral myocarditis.

    abstract：:CVB3 is a common human pathogen to be highly lethal to newborns and causes viral myocarditis and pancreatitis in adults. However, there is no vaccine available for clinical use. CVB3 capsid protein VP1 is an immunodominant structural protein, containing several B- and T-cell epitopes. However, immunization of mice wit...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep41485

    authors： Qi X,Xiong S

    更新日期：2017-02-02 00:00:00

  • Evolution of sex determination and heterogamety changes in section Otites of the genus Silene.

    abstract：:Switches in heterogamety are known to occur in both animals and plants. Although plant sex determination systems probably often evolved more recently than those in several well-studied animals, including mammals, and have had less time for switches to occur, we previously detected a switch in heterogamety in the plant...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-37412-x

    authors： Balounova V,Gogela R,Cegan R,Cangren P,Zluvova J,Safar J,Kovacova V,Bergero R,Hobza R,Vyskot B,Oxelman B,Charlesworth D,Janousek B

    更新日期：2019-01-31 00:00:00

  • Identifying molecular signatures of hypoxia adaptation from sex chromosomes: A case for Tibetan Mastiff based on analyses of X chromosome.

    abstract：:Genome-wide studies on high-altitude adaptation have received increased attention as a classical case of organismal evolution under extreme environment. However, the current genetic understanding of high-altitude adaptation emanated mainly from autosomal analyses. Only a few earlier genomic studies paid attention to t...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep35004

    authors： Wu H,Liu YH,Wang GD,Yang CT,Otecko NO,Liu F,Wu SF,Wang L,Yu L,Zhang YP

    更新日期：2016-10-07 00:00:00

  • Biogeographic parallels in thermal tolerance and gene expression variation under temperature stress in a widespread bumble bee.

    abstract：:Global temperature changes have emphasized the need to understand how species adapt to thermal stress across their ranges. Genetic mechanisms may contribute to variation in thermal tolerance, providing evidence for how organisms adapt to local environments. We determine physiological thermal limits and characterize ge...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-73391-8

    authors： Pimsler ML,Oyen KJ,Herndon JD,Jackson JM,Strange JP,Dillon ME,Lozier JD

    更新日期：2020-10-13 00:00:00

  • Epitopes in α8β1 and other RGD-binding integrins delineate classes of integrin-blocking antibodies and major binding loops in α subunits.

    abstract：:Identification of epitopes for integrin-blocking monoclonal antibodies (mAbs) has aided our understanding of structure-function relationship of integrins. We mapped epitopes of chicken anti-integrin-α8-subunit-blocking mAbs by mutational analyses, examining regions that harboured all mapped epitopes recognized by mAbs...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep13756

    authors： Nishimichi N,Kawashima N,Yokosaki Y

    更新日期：2015-09-09 00:00:00

  • β-Ginkgotides: Hyperdisulfide-constrained peptides from Ginkgo biloba.

    abstract：:Hyperdisulfide-constrained peptides are distinguished by their high stability and diverse functions. Thus far, these peptides have been reported from animals only but their occurrence in plants are rare. Here, we report the discovery, synthesis and characterization of a hyperdisulfide-constrained peptides family of ap...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-06598-x

    authors： Wong KH,Tan WL,Xiao T,Tam JP

    更新日期：2017-07-21 00:00:00

  • Characterisation of cuticular inflation development and ultrastructure in Trichuris muris using correlative X-ray computed tomography and electron microscopy.

    abstract：:The parasitic nematode Trichuris trichiura is a significant burden on public health in developing countries, and currently available drugs exhibit a poor cure rate. Worms live within a specialised tunnel of host intestinal epithelial cells and have anterior-ventral projections of the cuticle termed "cuticular inflatio...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-61916-0

    authors： O'Sullivan JDB,Cruickshank SM,Starborg T,Withers PJ,Else KJ

    更新日期：2020-04-03 00:00:00

  • The role of gel-phase domains in electroporation of vesicles.

    abstract：:Transient permeabilisation of the cell membrane is a critical step to introduce drugs or DNA into living cells, yet challenging for both biological research and therapeutic applications. To achieve this, electroporation (or electropermeabilisation) has become a widely used method due to its simplicity to deliver almos...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-23097-9

    authors： Perrier DL,Rems L,Kreutzer MT,Boukany PE

    更新日期：2018-03-19 00:00:00

  • Acoustic detection of DNA conformation in genetic assays combined with PCR.

    abstract：:Application of PCR to multiplexing assays is not trivial; it requires multiple fluorescent labels for amplicon detection and sophisticated software for data interpretation. Alternative PCR-free methods exploiting new concepts in nanotechnology exhibit high sensitivities but require multiple labeling and/or amplificati...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep02033

    authors： Papadakis G,Tsortos A,Kordas A,Tiniakou I,Morou E,Vontas J,Kardassis D,Gizeli E

    更新日期：2013-01-01 00:00:00

  • Lentiviral shRNA against KCa3.1 inhibits allergic response in allergic rhinitis and suppresses mast cell activity via PI3K/AKT signaling pathway.

    abstract：:Calcium-activated potassium ion channel-3.1 (KCa3.1) plays a pivotal role in the potassium-calcium exchange involved in atopy. This study aimed to explore the impact of lentiviral-mediated shRNA silencing KCa3.1 on allergic response in a murine allergic rhinitis (AR) model. The BALB/c mice were divided into four group...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep13127

    authors： Lin H,Zheng C,Li J,Yang C,Hu L

    更新日期：2015-08-14 00:00:00

  • No influence of eye gaze on emotional face processing in the absence of conscious awareness.

    abstract：:The human brain has evolved specialised mechanisms to enable the rapid detection of threat cues, including emotional face expressions (e.g., fear and anger). However, contextual cues - such as gaze direction - influence the ability to recognise emotional expressions. For instance, anger paired with direct gaze, and fe...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-52728-y

    authors： Caruana N,Inkley C,Zein ME,Seymour K

    更新日期：2019-11-07 00:00:00

  • An integrated platform for genome engineering and gene expression perturbation in Plasmodium falciparum.

    abstract：:Establishing robust genome engineering methods in the malarial parasite, Plasmodium falciparum, has the potential to substantially improve the efficiency with which we gain understanding of this pathogen's biology to propel treatment and elimination efforts. Methods for manipulating gene expression and engineering the...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-77644-4

    authors： Nasamu AS,Falla A,Pasaje CFA,Wall BA,Wagner JC,Ganesan SM,Goldfless SJ,Niles JC

    更新日期：2021-01-11 00:00:00

  • Deconvoluting the Photonic and Electronic Response of 2D Materials: The Case of MoS2.

    abstract：:Evaluating and tuning the properties of two-dimensional (2D) materials is a major focus of advancing 2D science and technology. While many claim that the photonic properties of a 2D layer provide evidence that the material is "high quality", this may not be true for electronic performance. In this work, we deconvolute...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-16970-6

    authors： Zhang K,Borys NJ,Bersch BM,Bhimanapati GR,Xu K,Wang B,Wang K,Labella M,Williams TA,Haque MA,Barnard ES,Fullerton-Shirey S,Schuck PJ,Robinson JA

    更新日期：2017-12-05 00:00:00

  • Novel Physical Vapor Deposition Approach to Hybrid Perovskites: Growth of MAPbI3 Thin Films by RF-Magnetron Sputtering.

    abstract：:Solution-based methods represent the most widespread approach used to deposit hybrid organic-inorganic perovskite films for low-cost but efficient solar cells. However, solution-process techniques offer limited control over film morphology and crystallinity, and most importantly do not allow sequential film deposition...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-33760-w

    authors： Bonomi S,Marongiu D,Sestu N,Saba M,Patrini M,Bongiovanni G,Malavasi L

    更新日期：2018-10-18 00:00:00

  • Amixicile, a novel strategy for targeting oral anaerobic pathogens.

    abstract：:The oral microflora is composed of both health-promoting as well as disease-initiating bacteria. Many of the disease-initiating bacteria are anaerobic and include organisms such as Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Tannerella forsythia. Here we investigated a novel therapeut...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-09616-0

    authors： Hutcherson JA,Sinclair KM,Belvin BR,Gui Q,Hoffman PS,Lewis JP

    更新日期：2017-09-05 00:00:00

  • Geometry-dependent band shift and dielectric modification of nanoporous Si nanowires.

    abstract：:In order to obtain a detailed understanding of the modulation of electronic properties in nanoporous Si (np-Si) nanowires with containing ordered, nanometer-sized cylindrical pores, we propose a theoretical method to clarify the band shift and associated with the dielectric modification determined by the geometrical p...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-14647-8

    authors： Yu WB,Ouyang G

    更新日期：2017-10-31 00:00:00

  • A clinical investigation treating different types of fibroids identified by MRI-T2WI imaging with ultrasound guided high intensity focused ultrasound.

    abstract：:Clinical data from 172 cases of uterine fibroids with different appearances on MRI-T2WI and accepted ultrasound guided high intensity focused ultrasound (USgHIFU) treatment were retrospectively analyzed. This study aimed to evaluate the clinical safety and efficacy of ablating different types of fibroids, classified b...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-11486-5

    authors： Zhao WP,Zhang J,Han ZY,Yao JP,Zhou X,Liang P

    更新日期：2017-09-07 00:00:00

  • Female Blow Flies As Vertebrate Resource Indicators.

    abstract：:Rapid vertebrate diversity evaluation is invaluable for monitoring changing ecosystems worldwide. Wild blow flies naturally recover DNA and chemical signatures from animal carcasses and feces. We demonstrate the power of blow flies as biodiversity monitors through sampling of flies in three environments with varying h...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-46758-9

    authors： Owings CG,Banerjee A,Asher TMD,Gilhooly WP 3rd,Tuceryan A,Huffine M,Skaggs CL,Adebowale IM,Manicke NE,Picard CJ

    更新日期：2019-07-22 00:00:00

  • Quantification of cell-bubble interactions in a 3D engineered tissue phantom.

    abstract：:Understanding cell-bubble interactions is crucial for preventing bubble related pathologies and harnessing their potential therapeutic benefits. Bubbles can occur in the body as a result of therapeutic intravenous administration, surgery, infections or decompression. Subsequent interactions with living cells, may resu...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-06678-y

    authors： Walsh C,Ovenden N,Stride E,Cheema U

    更新日期：2017-07-24 00:00:00

  • PKC-ѳ is dispensable for OX40L-induced TCR-independent Treg proliferation but contributes by enabling IL-2 production from effector T-cells.

    abstract：:We have previously shown that OX40L/OX40 interaction is critical for TCR-independent selective proliferation of Foxp3+ Tregs, but not Foxp3- effector T-cells (Teff), when CD4+ T-cells are co-cultured with GM-CSF derived bone marrow dendritic cells (G-BMDCs). Events downstream of OX40L/OX40 interaction in Tregs respons...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-05254-8

    authors： Alharshawi K,Marinelarena A,Kumar P,El-Sayed O,Bhattacharya P,Sun Z,Epstein AL,Maker AV,Prabhakar BS

    更新日期：2017-07-26 00:00:00