当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Location of pyridoxal phosphate in glycogen phosphorylase a.

Location of pyridoxal phosphate in glycogen phosphorylase a.

Abstract：

:The pyridoxal 5'-phosphate cofactor of glycogen phosphorylase a (1,4-alpha-D-glucan:orthophosphate alpha-glucosyltransferase, EC2.4.1.1.) has been positioned on the protomer with x-ray diffraction data, chemical markers, and sequence information. The electron density was computed from 3.0-A resolution phases calculated from four heavy-atom derivatives. The cofactor is buried inside the protomer adjacent to the glucose-binding site. The phosphoryl substrates Pi and glucose-1-P each bind at two sites on the protomer. At low concentrations, Pi and glucose-1-P bind in the same location as does the allosteric effector AMP, near the monomer-monomer interface and some 30 A from the glucose site. At high concentrations glucose-1-P also binds strongly at the glucose site, with its phosphate only 7.2 A from that of the cofactor. Inorganic phosphate can also bind at this site. Implications for the participation of the pyridoxal phosphate in the catalytic mechanism are discussed in the light of these structural findings as well as the wealth of indirect evidence in the literature.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Sygusch J,Madsen NB,Kasvinsky PJ,Fletterick RJ

doi

10.1073/pnas.74.11.4757

subject

Has Abstract

pub_date

1977-11-01 00:00:00

pages

4757-61

issue

11

eissn

0027-8424

issn

1091-6490

journal_volume

74

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Transient retinoic acid signaling confers anterior-posterior polarity to the inner ear.

    abstract：:Vertebrate hearing and balance are based in complex asymmetries of inner ear structure. Here, we identify retinoic acid (RA) as an extrinsic signal that acts directly on the ear rudiment to affect its compartmentalization along the anterior-posterior axis. A rostrocaudal wave of RA activity, generated by tissues surro...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1010547108

    authors： Bok J,Raft S,Kong KA,Koo SK,Dräger UC,Wu DK

    更新日期：2011-01-04 00:00:00

  • Relationship between in vivo degradative rates and isoelectric points of proteins.

    abstract：:Previous studies have shown that in mammalian cells proteins of large molecular weight are degraded more rapidly than small ones. Evidence is presented here that half-lives of proteins are also related to their isoelectric points. A double-isotope method was used to compare degradative rates of soluble proteins separa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.10.3893

    authors： Dice JF,Goldberg AL

    更新日期：1975-10-01 00:00:00

  • Homozygosity and patch structure in plant populations as a result of nearest-neighbor pollination.

    abstract：:The population genetic consequences of nearest-neighbor pollination in an outcrossing plant species were investigated through computer simulations. The genetic system consisted of two alleles at a single locus in a self-incompatible plant that mates by random pollen transfer from a neighboring individual. Beginning wi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.1.203

    authors： Turner ME,Stephens JC,Anderson WW

    更新日期：1982-01-01 00:00:00

  • Nonsensory target-dependent organization of piriform cortex.

    abstract：:The piriform cortex (PCX) is the largest component of the olfactory cortex and is hypothesized to be the locus of odor object formation. The distributed odorant representation found in PCX contrasts sharply with the topographical representation seen in other primary sensory cortices, making it difficult to test this v...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1411266111

    authors： Chen CF,Zou DJ,Altomare CG,Xu L,Greer CA,Firestein SJ

    更新日期：2014-11-25 00:00:00

  • Role of structural determinants in folding of the sandwich-like protein Pseudomonas aeruginosa azurin.

    abstract：:An invariant substructure that forms two interlocked pairs of neighboring beta-strands occurs in essentially all known sandwich-like proteins. Eight conserved positions in these strands were recently shown to act as structural determinants. To test whether the residues at these invariant positions are conserved for me...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0501038102

    authors： Wilson CJ,Wittung-Stafshede P

    更新日期：2005-03-15 00:00:00

  • Interplay of approximate planning strategies.

    abstract：:Humans routinely formulate plans in domains so complex that even the most powerful computers are taxed. To do so, they seem to avail themselves of many strategies and heuristics that efficiently simplify, approximate, and hierarchically decompose hard tasks into simpler subtasks. Theoretical and cognitive research has...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1414219112

    authors： Huys QJ,Lally N,Faulkner P,Eshel N,Seifritz E,Gershman SJ,Dayan P,Roiser JP

    更新日期：2015-03-10 00:00:00

  • Stability of dense liquid carbon dioxide.

    abstract：:We present ab initio calculations of the phase diagram of liquid CO(2) and its melting curve over a wide range of pressure and temperature conditions, including those relevant to the Earth. Several distinct liquid phases are predicted up to 200 GPa and 10,000 K based on their structural and electronic characteristics....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1120243109

    authors： Boates B,Teweldeberhan AM,Bonev SA

    更新日期：2012-09-11 00:00:00

  • GWAS with principal component analysis identifies a gene comprehensively controlling rice architecture.

    abstract：:Elucidation of the genetic control of rice architecture is crucial due to the global demand for high crop yields. Rice architecture is a complex trait affected by plant height, tillering, and panicle morphology. In this study, principal component analysis (PCA) on 8 typical traits related to plant architecture reveale...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1904964116

    authors： Yano K,Morinaka Y,Wang F,Huang P,Takehara S,Hirai T,Ito A,Koketsu E,Kawamura M,Kotake K,Yoshida S,Endo M,Tamiya G,Kitano H,Ueguchi-Tanaka M,Hirano K,Matsuoka M

    更新日期：2019-10-15 00:00:00

  • Tuning the reactivity of semiconductor surfaces by functionalization with amines of different basicity.

    abstract：:Surface functionalization of semiconductors has been the backbone of the newest developments in microelectronics, energy conversion, sensing device design, and many other fields of science and technology. Over a decade ago, the notion of viewing the surface itself as a chemical reagent in surface reactions was introdu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1006656107

    authors： Bent SF,Kachian JS,Rodríguez-Reyes JC,Teplyakov AV

    更新日期：2011-01-18 00:00:00

  • Distinct metastable atmospheric regimes despite nearly Gaussian statistics: a paradigm model.

    abstract：:A controversial topic in the recent climate modeling literature is the fashion in which metastable low-frequency regimes in the atmosphere occur despite nearly Gaussian statistics for these planetary waves. Here a simple 57-mode paradigm model for such metastable atmospheric regime behavior is introduced and analyzed ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0602641103

    authors： Majda AJ,Franzke CL,Fischer A,Crommelin DT

    更新日期：2006-05-30 00:00:00

  • General methodology for inferring failure-spreading dynamics in networks.

    abstract：:A generic modeling framework to infer the failure-spreading process based on failure times of individual nodes is proposed and tested in four simulation studies: one for cascading failures in interdependent power and transportation networks, one for influenza epidemics, one benchmark test case for congestion cascade i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1722313115

    authors： Guan X,Chen C

    更新日期：2018-08-28 00:00:00

  • Rhesus monkey placental transgene expression after lentiviral gene transfer into preimplantation embryos.

    abstract：:Transgenic mice have provided invaluable information about gene function and regulation. However, because of marked differences between rodents and primates, some areas of human biology such as early embryonic development, aging, and maternal-fetal interactions would be best studied in a nonhuman primate model. Here, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.181336098

    authors： Wolfgang MJ,Eisele SG,Browne MA,Schotzko ML,Garthwaite MA,Durning M,Ramezani A,Hawley RG,Thomson JA,Golos TG

    更新日期：2001-09-11 00:00:00

  • Identified circuit in rat postrhinal cortex encodes essential information for performing specific visual shape discriminations.

    abstract：:Learning theories hypothesize specific circuits encode essential information for performance. For simple tasks in invertebrates and mammals, the essential circuits are known, but for cognitive functions, the essential circuits remain unidentified. Here, we show that some essential information for performing a choice t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0912950107

    authors： Zhang GR,Cao H,Kong L,O'Brien J,Baughns A,Jan M,Zhao H,Wang X,Lu XG,Cook RG,Geller AI

    更新日期：2010-08-10 00:00:00

  • A rapid and specific method for isolation of thiol-containing peptides from large proteins by thiol-disulfide exchange on a solid support.

    abstract：:Activated thiol-Sepharose [agarose-(glutathione-2-pyridyl disulfide) conjugate] has been used to immobilize proteins with a single or a few thiol groups via disulfide bridges. The immobilized proteins were subsequently proteolytically degraded. After washing, the thiol-containing peptides were eluted with a reducing a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.8.3029

    authors： Egorov TA,Svenson A,Rydén L,Carlsson J

    更新日期：1975-08-01 00:00:00

  • DNA of vegetative bacteriophage lambda. VI. Electron microscopic studies of replicating lambda DNA.

    abstract：:Electron micrographs of replicating lambda DNA molecules, during the period of progeny-particle DNA formation, show single-tailed circular structures. The tails are, predominantly, shorter than one viral genome. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.68.1.112

    authors： Kiger JA Jr,Sinsheimer RL

    更新日期：1971-01-01 00:00:00

  • Local apoptotic-like mechanisms underlie complement-mediated synaptic pruning.

    abstract：:C1q, a member of the immune complement cascade, is implicated in the selective pruning of synapses by microglial phagocytosis. C1q-mediated synapse elimination has been shown to occur during brain development, while increased activation and complement-dependent synapse loss is observed in neurodegenerative diseases. H...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1722613115

    authors： Györffy BA,Kun J,Török G,Bulyáki É,Borhegyi Z,Gulyássy P,Kis V,Szocsics P,Micsonai A,Matkó J,Drahos L,Juhász G,Kékesi KA,Kardos J

    更新日期：2018-06-12 00:00:00

  • Impact of natural genetic variation on the transcriptome of autotetraploid Arabidopsis thaliana.

    abstract：:Polyploidy, the presence of more than two complete sets of chromosomes in an organism, has significantly shaped the genomes of angiosperms during evolution. Two forms of polyploidy are often considered: allopolyploidy, which originates from interspecies hybrids, and autopolyploidy, which originates from intraspecies g...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1000852107

    authors： Yu Z,Haberer G,Matthes M,Rattei T,Mayer KF,Gierl A,Torres-Ruiz RA

    更新日期：2010-10-12 00:00:00

  • Convergent evolution toward an improved growth rate and a reduced resistance range in Prochlorococcus strains resistant to phage.

    abstract：:Prochlorococcus is an abundant marine cyanobacterium that grows rapidly in the environment and contributes significantly to global primary production. This cyanobacterium coexists with many cyanophages in the oceans, likely aided by resistance to numerous co-occurring phages. Spontaneous resistance occurs frequently i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1420347112

    authors： Avrani S,Lindell D

    更新日期：2015-04-28 00:00:00

  • Coexpression of NOS2 and COX2 accelerates tumor growth and reduces survival in estrogen receptor-negative breast cancer.

    abstract：:Proinflammatory signaling pathways are commonly up-regulated in breast cancer. In estrogen receptor-negative (ER-) and triple-negative breast cancer (TNBC), nitric oxide synthase-2 (NOS2) and cyclooxygenase-2 (COX2) have been described as independent predictors of disease outcome. We further explore these findings by ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1709119114

    authors： Basudhar D,Glynn SA,Greer M,Somasundaram V,No JH,Scheiblin DA,Garrido P,Heinz WF,Ryan AE,Weiss JM,Cheng RYS,Ridnour LA,Lockett SJ,McVicar DW,Ambs S,Wink DA

    更新日期：2017-12-05 00:00:00

  • Pupil response as a predictor of blindsight in hemianopia.

    abstract：:Significantly above-chance detection of stimuli presented within the field defect of patients with postgeniculate lesions is termed "blindsight." It has been proposed that those with blindsight are more likely to benefit from visual rehabilitation by repeated stimulation, leading to increased visual sensitivity within...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1318395110

    authors： Sahraie A,Trevethan CT,MacLeod MJ,Urquhart J,Weiskrantz L

    更新日期：2013-11-05 00:00:00

  • The role of long-range connections on the specificity of the macaque interareal cortical network.

    abstract：:We investigated the influence of interareal distance on connectivity patterns in a database obtained from the injection of retrograde tracers in 29 areas distributed over six regions (occipital, temporal, parietal, frontal, prefrontal, and limbic). One-third of the 1,615 pathways projecting to the 29 target areas were...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1218972110

    authors： Markov NT,Ercsey-Ravasz M,Lamy C,Ribeiro Gomes AR,Magrou L,Misery P,Giroud P,Barone P,Dehay C,Toroczkai Z,Knoblauch K,Van Essen DC,Kennedy H

    更新日期：2013-03-26 00:00:00

  • Mitogen-activated protein kinase pathways defend against bacterial pore-forming toxins.

    abstract：:Cytolytic pore-forming toxins are important for the virulence of many disease-causing bacteria. How target cells molecularly respond to these toxins and whether or not they can mount a defense are poorly understood. By using microarrays, we demonstrate that the nematode Caenorhabditis elegans responds robustly to Cry5...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0404073101

    authors： Huffman DL,Abrami L,Sasik R,Corbeil J,van der Goot FG,Aroian RV

    更新日期：2004-07-27 00:00:00

  • Nuclear magnetic resonance study of the globular domain of chicken histone H5: resonance assignment and secondary structure.

    abstract：:A 1H NMR study of the 79-residue globular domain of chicken erythrocyte histone H5 (GH5) is presented. Using a combination of two-dimensional NMR techniques to demonstrate through-bond and through-space (less than 5 A) connectivities, the resonances of GH5 are assigned in a sequential manner. From a qualitative interp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.20.7628

    authors： Zarbock J,Clore GM,Gronenborn AM

    更新日期：1986-10-01 00:00:00

  • Kappa-on-Heavy (KoH) bodies are a distinct class of fully-human antibody-like therapeutic agents with antigen-binding properties.

    abstract：:We describe a Kappa-on-Heavy (KoH) mouse that produces a class of highly diverse, fully human, antibody-like agents. This mouse was made by replacing the germline variable sequences of both the Ig heavy-chain (IgH) and Ig kappa (IgK) loci with the human IgK germline variable sequences, producing antibody-like molecule...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1901734117

    authors： Macdonald LE,Meagher KA,Franklin MC,Levenkova N,Hansen J,Badithe AT,Zhong M,Krueger P,Rafique A,Tu N,Shevchuk J,Wadhwa S,Ehrlich G,Bautista J,Grant C,Esau L,Poueymirou WT,Auerbach W,Morton L,Babb R,Chen G,Huang

    更新日期：2020-01-07 00:00:00

  • Increasing intracellular magnesium levels with the 31-amino acid MgtS protein.

    abstract：:Synthesis of the 31-amino acid, inner membrane protein MgtS (formerly denoted YneM) is induced by very low Mg2+ in a PhoPQ-dependent manner in Escherichia coli Here we report that MgtS acts to increase intracellular Mg2+ levels and maintain cell integrity upon Mg2+ depletion. Upon development of a functional tagged de...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1703415114

    authors： Wang H,Yin X,Wu Orr M,Dambach M,Curtis R,Storz G

    更新日期：2017-05-30 00:00:00

  • Starvation after AgRP neuron ablation is independent of melanocortin signaling.

    abstract：:Ablation of inhibitory agouti-related protein (AgRP)-expressing neurons in the arcuate nucleus that also synthesize gamma-amino-butyric acid (GABA) and neuropeptide Y in adult mice leads to starvation within 1 week. The removal of inhibition from the AgRP neurons onto neighboring proopiomelanocortin neurons and their ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0712062105

    authors： Wu Q,Howell MP,Cowley MA,Palmiter RD

    更新日期：2008-02-19 00:00:00

  • Latent luciferase activity in the fruit fly revealed by a synthetic luciferin.

    abstract：:Beetle luciferases are thought to have evolved from fatty acyl-CoA synthetases present in all insects. Both classes of enzymes activate fatty acids with ATP to form acyl-adenylate intermediates, but only luciferases can activate and oxidize d-luciferin to emit light. Here we show that the Drosophila fatty acyl-CoA syn...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1319300111

    authors： Mofford DM,Reddy GR,Miller SC

    更新日期：2014-03-25 00:00:00

  • Resistance to apoptosis caused by PIG-A gene mutations in paroxysmal nocturnal hemoglobinuria.

    abstract：:Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal hematopoietic stem cell disorder resulting from mutations in an X-linked gene, PIG-A, that encodes an enzyme required for the first step in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. PIG-A mutations result in absent or decreased cell surface ex...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.16.8756

    authors： Brodsky RA,Vala MS,Barber JP,Medof ME,Jones RJ

    更新日期：1997-08-05 00:00:00

  • Drosophila retrotransposon promoter includes an essential sequence at the initiation site and requires a downstream sequence for full activity.

    abstract：:We describe a 98-base-pair region (-38 to +60) in the long terminal repeat of the Drosophila gypsy retrotransposon that is sufficient for accurate normal-level transcription. We find that, unlike most RNA polymerase II (pol II) promoters, the gypsy promoter includes downstream sequences that are required for full acti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.1.102

    authors： Jarrell KA,Meselson M

    更新日期：1991-01-01 00:00:00

  • Effects of systemic multiexon skipping with peptide-conjugated morpholinos in the heart of a dog model of Duchenne muscular dystrophy.

    abstract：:Duchenne muscular dystrophy (DMD) is a lethal genetic disorder caused by an absence of the dystrophin protein in bodywide muscles, including the heart. Cardiomyopathy is a leading cause of death in DMD. Exon skipping via synthetic phosphorodiamidate morpholino oligomers (PMOs) represents one of the most promising ther...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1613203114

    authors： Echigoya Y,Nakamura A,Nagata T,Urasawa N,Lim KRQ,Trieu N,Panesar D,Kuraoka M,Moulton HM,Saito T,Aoki Y,Iversen P,Sazani P,Kole R,Maruyama R,Partridge T,Takeda S,Yokota T

    更新日期：2017-04-18 00:00:00