Effects of peptide ratios in nanofibre-based hydrogels for the prevention of capsular opacification.

Abstract:

PURPOSE:To moderate the capsular opacification (CO) response after lens surgery, an experimental study was performed in which nanofibre-based hydrogels (nanogels) with different ratios of attached peptides were applied to provide extracellular matrix-related cues for lens epithelial cells (LECs) in a porcine eye model. METHODS:The lens content was removed, and the capsules were refilled with nanogel. Lenses were divided into two groups, the first group (n = 34) was refilled with nanogels containing different ratios of two laminin-derived peptides (IKVAV + YIGSR), and the latter group (n = 26) was refilled with nanogel combinations of a fibronectin-derived and a type IV collagen-derived peptide (RGDS + DGEA). Two lenses were refilled with culture medium to investigate the effect of the medium on LECs. After refilling, lenses were extracted and cultured for 3 weeks. Lens epithelial cells (LECs) were assessed for morphology and alpha-smooth muscle actin (αSMA) expression using confocal laser scanning microscopy. RESULTS:Differences were seen in cell morphology between lenses refilled with nanogels with IKVAV + YIGSR and RGDS + DGEA peptides. In nanogels with IKVAV + YIGSR peptides, differences in LEC morphology were largest when ratios between the peptides were unequal, whereas LEC responses from the RGDS + DGEA refilled groups showed variation in LEC morphology dependent on the total quantity of mixed-in peptides. The culture medium did not induce proliferation or transformation of LECs. CONCLUSIONS:Ratios and concentrations of cell adhesion-mediating peptides both can direct the LEC response, depending on the adhesion molecules of origin, by influencing LEC proliferation and transformation. Nanogels with incorporated peptides may be tuned towards CO prevention.

journal_name

Acta Ophthalmol

journal_title

Acta ophthalmologica

authors

Nibourg LM,Gelens E,Nibourg SA,de Jong MR,Kuijer R,van Kooten TG,Koopmans SA

doi

10.1111/aos.13047

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

721-729

issue

7

eissn

1755-375X

issn

1755-3768

journal_volume

94

pub_type

杂志文章
  • Multicenter clinical trial of high-intensity focused ultrasound treatment in glaucoma patients without previous filtering surgery.

    abstract:PURPOSE:To evaluate the efficacy and safety of the ultrasonic circular cyclocoagulation procedure in patients with open-angle glaucoma naïve of previous filtering surgery. METHODS:Prospective non-comparative interventional clinical study conducted in five French University Hospitals. Thirty eyes of 30 patients with op...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1111/aos.12913

    authors: Aptel F,Denis P,Rouland JF,Renard JP,Bron A

    更新日期:2016-08-01 00:00:00

  • Correlation between retinal vein occlusion and cancer - a nationwide Danish cohort study.

    abstract:PURPOSE:To explore the association between retinal vein occlusion (RVO) and incident cancer. METHODS:All Danish citizens with a first-time diagnosis of RVO and no previous diagnosis of cancer in the period from 1 January 2004 to 31 December 2014 were included. Five likewise cancer-free, age- and gender-matched control...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章,多中心研究

    doi:10.1111/aos.13860

    authors: Toft-Petersen AP,Muttuvelu DV,Heegaard S,Torp-Pedersen C

    更新日期:2018-12-01 00:00:00

  • Plusoptix photoscreener use for paediatric vision screening in Flanders and Iran.

    abstract:PURPOSE:Photoscreening assesses risk factors for amblyopia, as an alternative to measurement of visual acuity (VA) to detect amblyopia, on the premise that its early correction could prevent development of amblyopia. We studied implementations of Plusoptix photoscreening in existing population-based screening in Flande...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章,评审

    doi:10.1111/aos.14144

    authors: Bostamzad P,Horwood AM,Schalij-Delfos NE,Boelaert K,de Koning HJ,Simonsz HJ

    更新日期:2020-02-01 00:00:00

  • Dexamethasone implant with fixed or individualized regimen in the treatment of diabetic macular oedema: six-month outcomes of the UDBASA study.

    abstract:PURPOSE:To evaluate a pro re nata administration of Ozurdex® implant versus a single administration for treating diabetic macular oedema (DME). METHODS:This exploratory study is designed as a comparative, multicentre, randomized study with a follow-up of 6 months. Patients with DME were assigned to treatment at baseli...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.1111/aos.13395

    authors: Sarao V,Veritti D,Furino C,Giancipoli E,Alessio G,Boscia F,Lanzetta P

    更新日期:2017-06-01 00:00:00

  • Anisometropia of ocular refractive and biometric measures among 66- to 79-year-old female twins.

    abstract:PURPOSE:To examine the prevalence of anisometropia of spherical refraction (AnisoSR), astigmatism (AnisoAST) and spherical equivalent (AnisoSE) and their associations with spherical refraction (SR), refractive astigmatism (AST), spherical equivalent (SE) and interocular differences of ocular biometric parameters among ...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.13125

    authors: Pärssinen O,Kauppinen M,Kaprio J,Rantanen T

    更新日期:2016-12-01 00:00:00

  • Risk factors for the hypertensive phase after implantation of a glaucoma drainage device.

    abstract:PURPOSE:To investigate risk factors for the development of the hypertensive phase after implantation of the Ahmed glaucoma valve (AGV). METHODS:A total of 128 patients who had undergone AGV implantation were included in this study. The hypertensive phase was defined as intraocular pressure (IOP) > 21 mmHg during the f...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.12916

    authors: Jung KI,Park CK

    更新日期:2016-08-01 00:00:00

  • Treatment patterns, visual acuity and quality-of-life outcomes of the WAVE study - a noninterventional study of ranibizumab treatment for neovascular age-related macular degeneration in Germany.

    abstract:PURPOSE:To evaluate effectiveness, tolerability and safety of repeated intravitreal injections of 0.5 mg ranibizumab for the treatment of neovascular age-related macular degeneration in routine medical practice in Germany. METHODS:A noninterventional study with 3470 patients treated in 274 medical centres according to...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章,多中心研究

    doi:10.1111/j.1755-3768.2012.02493.x

    authors: Finger RP,Wiedemann P,Blumhagen F,Pohl K,Holz FG

    更新日期:2013-09-01 00:00:00

  • Recurrent rearrangements of the PLAG1 and HMGA2 genes in lacrimal gland pleomorphic adenoma and carcinoma ex pleomorphic adenoma.

    abstract:PURPOSE:Lacrimal gland tumours constitute a wide spectrum of neoplastic lesions that are histologically similar to tumours of the salivary gland. In the salivary gland, pleomorphic adenoma (PA) is frequently characterized by recurrent chromosomal rearrangements of the PLAG1 and HMGA2 genes, a genetic feature retained i...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.13667

    authors: Andreasen S,von Holstein SL,Homøe P,Heegaard S

    更新日期:2018-11-01 00:00:00

  • Refraction and visual acuity in a national Danish cohort of 4-year-old children of extremely preterm delivery.

    abstract:PURPOSE:A recent threefold increase in laser treatment for advanced retinopathy of prematurity (ROP) triggered a nationwide preschool ophthalmic and developmental status among extremely preterm survivors. Here, we discuss refraction and visual acuity. METHODS:Survivors (n = 178) from a national birth cohort (February ...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.12643

    authors: Fledelius HC,Bangsgaard R,Slidsborg C,laCour M

    更新日期:2015-06-01 00:00:00

  • Seven-day storage of pneumatically dissected Descemet's endothelial grafts with and without Dua's layer.

    abstract:PURPOSE:To evaluate the efficacy of big-bubble (BB) technique in separating Dua's layer together with the Descemet's membrane endothelial (DE) graft and the effect of 7-day storage of the prepared tissues on the endothelial cells. METHOD:This is an experimental study in which 21 human corneo-scleral tissues unsuitable...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.12823

    authors: GamalElDin SA,Salama MM,ElShazly MI

    更新日期:2016-03-01 00:00:00

  • Anisometropia of spherical equivalent and astigmatism among myopes: a 23-year follow-up study of prevalence and changes from childhood to adulthood.

    abstract:PURPOSE:To study anisometropia of spherical equivalent and astigmatism from the onset of myopia at school age to adulthood. METHODS:A total of 240 myopic schoolchildren (mean age 10.9 years), with no previous spectacles, were recruited during 1983-1984 to a randomized 3-year clinical trial of bifocal treatment of myop...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章,随机对照试验

    doi:10.1111/aos.13405

    authors: Pärssinen O,Kauppinen M

    更新日期:2017-08-01 00:00:00

  • Efficacy and safety of recombinant tissue plasminogen activator and gas versus bevacizumab and gas for subretinal haemorrhage.

    abstract:PURPOSE:To report the 12 months efficacy of initial intravitreal bevacizumab or intravitreal recombinant tissue plasminogen activator (rtPA) combined with expansile gas in patients with subretinal haemorrhage caused by neovascular age-related macular degeneration (AMD). METHODS:Forty-five eyes of 45 patients with subr...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/j.1755-3768.2011.02264.x

    authors: Mayer WJ,Hakim I,Haritoglou C,Gandorfer A,Ulbig M,Kampik A,Wolf A

    更新日期:2013-05-01 00:00:00

  • Retinal vascular fractal dimension and cerebral blood flow, a pilot study.

    abstract:PURPOSE:Ocular and brain microcirculation share embryological and histological similarities. The retinal vascular fractal dimension (FD) is a marker of retinal vascular complexity of the vascular tree. The purpose of this study was to explore the relationship between cerebral blood flow (CBF), retinal vascular FD and o...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.14232

    authors: Nadal J,Deverdun J,de Champfleur NM,Carriere I,Creuzot-Garcher C,Delcourt C,Chiquet C,Kawasaki R,Villain M,Ritchie K,Le Bars E,Daien V

    更新日期:2020-02-01 00:00:00

  • The effect of deep sclerectomy on intraocular pressure of normal-tension glaucoma patients: 1-year results.

    abstract:PURPOSE:To study the intraocular pressure (IOP)-reducing effect of deep sclerectomy on normal-tension glaucoma (NTG) patients. METHODS:We retrospectively analysed 21 eyes of 18 consecutive NTG patients who had undergone deep sclerectomy with mitomycin-C and a collagen implant. RESULTS:Median (range) preoperative IOP ...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/j.1755-3768.2009.01623.x

    authors: Suominen S,Harju M,Ihanamäki T,Vesti E

    更新日期:2010-02-01 00:00:00

  • Systemic use of calcium channel blockers associated with less increase in central retinal thickness after uncomplicated cataract surgery.

    abstract:PURPOSE:To examine the role of systemic medication on the risk of pseudophakic cystoid macular edema (PCME) following uneventful cataract surgery. METHODS:A total of 269 eyes undergoing routine cataract surgery. Spectral-domain optical coherence tomography imaging was conducted before surgery and at 28 days. Informati...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.13911

    authors: Danni R,Taipale C,Holmström EJ,Ilveskoski L,Tuuminen R

    更新日期:2019-03-01 00:00:00

  • Homocysteine and risk of age-related macular degeneration: a systematic review and meta-analysis.

    abstract::There is still no agreement on total plasma homocysteine (tHcy) role in age-related macular degeneration (AMD), the leading cause of new blindness in industrialized countries. We performed a systematic review and meta-analysis of the published data on the correlation between tHcy and AMD. MEDLINE/PubMed and ISI Web of...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章,meta分析,评审

    doi:10.1111/aos.13343

    authors: Pinna A,Zaccheddu F,Boscia F,Carru C,Solinas G

    更新日期:2018-05-01 00:00:00

  • Initial observation or treatment for diabetic macular oedema with good visual acuity: two-year outcomes comparison in routine clinical practice: data from the Fight Retinal Blindness! Registry.

    abstract:PURPOSE:To compare visual acuity (VA) change at 24 months in eyes with clinically significant DME (CSDME) and good VA initially treated versus initially observed in routine clinical practice. METHODS:Retrospective analysis of treatment-naïve eyes with CSDME and good VA (baseline VA ≥ 79 letters), with at least 24 mont...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.14672

    authors: Gabrielle PH,Nguyen V,Bhandari S,Mehta H,Viola F,Arnold J,Fraser-Bell S,Barthelmes D,Creuzot-Garcher C,Gillies M

    更新日期:2020-11-16 00:00:00

  • Ophthalmic experience over 10 years in an observational nationwide Danish cohort of Fabry patients with access to enzyme replacement.

    abstract:PURPOSE:Enzyme replacement therapy (ERT) was offered from year 2001 to patients with Fabry disease. The ophthalmic experience was analysed, as part of a general 10-year status. METHODS:A retrospective observational series comprising 39 patients (25 females, 14 males) closely followed by the endocrinologists, and with ...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.12588

    authors: Fledelius HC,Sandfeld L,Rasmussen ÅK,Madsen CV,Feldt-Rasmussen U

    更新日期:2015-05-01 00:00:00

  • Human bone marrow mesenchymal stem cells for retinal vascular injury.

    abstract:PURPOSE:To examine the potential of intravitreally implanted human bone marrow-derived mesenchymal stem cells (BMSCs) to affect vascular repair and the blood-retina barrier in mice and rats with oxygen-induced retinopathy, diabetic retinopathy or retinal ischaemia-reperfusion damage. METHODS:Three study groups (oxygen...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.13154

    authors: Wang JD,An Y,Zhang JS,Wan XH,Jonas JB,Xu L,Zhang W

    更新日期:2017-09-01 00:00:00

  • Half-dose photodynamic therapy followed by diode micropulse laser therapy as treatment for chronic central serous chorioretinopathy: evaluation of a prospective treatment protocol.

    abstract:PURPOSE:To evaluate the outcome of a prospective protocol for the treatment of chronic central serous chorioretinopathy (CSC). METHODS:Interventional prospective case series in 59 eyes (59 patients) with active chronic CSC. All patients were first treated with indocyanine green angiography (ICGA)-guided half-dose phot...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.12938

    authors: Breukink MB,Mohr JK,Ossewaarde-van Norel A,den Hollander AI,Keunen JE,Hoyng CB,Boon CJ

    更新日期:2016-03-01 00:00:00

  • Correlation between Genotype and Phenotype in 69 Chinese Patients with USH2A Mutations: A comparative study of the patients with Usher Syndrome and Nonsyndromic Retinitis Pigmentosa.

    abstract:PURPOSE:The aim of this study was to analyse 69 Chinese patients with USH2A mutations and to assess the genotype-phenotype correlation. METHODS:All 36 Usher syndrome type IIA patients and 33 nonsyndromic RP (retinitis pigmentosa) patients underwent clinical examinations. Eye examinations included best-corrected visual...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.14626

    authors: Meng X,Liu X,Li Y,Guo T,Yang L

    更新日期:2020-10-29 00:00:00

  • Natural course of the intraretinal hyperreflective sign after macular haemorrhage absorption in eyes with pathologic myopia.

    abstract:PURPOSE:We investigated the natural course of the intraretinal hyperreflective (IRH) sign after macular haemorrhage (MHE) absorption in eyes with pathologic myopia. METHODS:This prospective observational cohort study evaluated 28 patients with pathologic myopia and MHE. The eyes were categorized into IRH and non-IRH s...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.14332

    authors: Hung KC,Wang SW,Hsia Y,Chen MS,Tsai CY,Ho TC

    更新日期:2019-12-06 00:00:00

  • Visual complaints of patients with glaucoma and controls under optimal and extreme luminance conditions.

    abstract:PURPOSE:To determine (i) whether, compared to controls, visual complaints of glaucoma patients are more pronounced under extreme luminance conditions than in the optimal luminance condition and (ii) whether complaints belonging to different extreme luminance conditions are associated. METHODS:We developed a luminance-...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.13695

    authors: Bierings RAJM,van Sonderen FLP,Jansonius NM

    更新日期:2018-05-01 00:00:00

  • In vivo biometric evaluation of Schlemm's canal with spectral-domain optical coherence tomography in pseuduexfoliation glaucoma.

    abstract:PURPOSE:To perform in vivo imaging of the Schlemm's canal (SC) with anterior segment spectral-domain optical coherence tomography [AS-spectral-domain (SD)-OCT] and also to measure its biometric parameters including the SC length and SC area in patients with pseudoexfoliation (PEX) glaucoma. METHODS:Forty-one consecuti...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.13080

    authors: Imamoglu S,Sevim MS,Alpogan O,Ercalik NY,Kumral ET,Pekel G,Bardak H

    更新日期:2016-12-01 00:00:00

  • Preoperative and postoperative measurements of retinal vessel oxygen saturation in patients with different grades of cataracts.

    abstract:PURPOSE:To assess the retinal vessel blood oxygen saturation preoperatively and postoperatively in patients with different grades of cataracts. METHODS:This study included 40 eyes from 40 patients with senile cataracts ranging from grade 1 to 3. All 40 eyes received standard phacoemulsification and intraocular lens im...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.13332

    authors: Chen H,Lin H,Chen W,Zhang B,Xiang W,Gao Q,Chen W,Liu Y

    更新日期:2017-09-01 00:00:00

  • Difference in the posterior pole profiles associated with the initial location of visual field defect in early-stage normal tension glaucoma.

    abstract:PURPOSE:To identify the differences in posterior pole profiles between early normal tension glaucoma (NTG) patients with an initial central visual field (VF) loss and those with a peripheral VF loss. METHODS:Normal tension glaucoma patients with parafoveal scotoma (PFS; within a central 10° of fixation; superior, n=47...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.12492

    authors: Choi JA,Park HY,Park CK

    更新日期:2015-03-01 00:00:00

  • Treatment of age-related macular degeneration after cataract surgery: a study from the Swedish National Cataract and Macula Registers.

    abstract:PURPOSE:To characterize pre- and perioperative factors associated with treatment for wet age-related macular degeneration (wet AMD) after cataract surgery. METHODS:This register-based cohort study with data from the Swedish National Cataract Register (NCR) and the Swedish Macula Register (SMR) from 2010 to 2017 compar...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.14519

    authors: Westborg I,Albrecht S,Granstam E,Karlsson N,Kugelberg M,Lundström M,Montan P,Behndig A

    更新日期:2020-06-23 00:00:00

  • Corneal biomechanical characteristics measured by the CorVis Scheimpflug technology in eyes with primary open-angle glaucoma and normal eyes.

    abstract:PURPOSE:To evaluate the biomechanical properties of the cornea using Corneal Visualization Scheimpflug Technology (CorVis ST, Oculus) in eyes with primary open-angle glaucoma (POAG) and normal control eyes. METHODS:A comparative cross-sectional study that included 42 patients with POAG and 60 normal control subjects m...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.12672

    authors: Tian L,Wang D,Wu Y,Meng X,Chen B,Ge M,Huang Y

    更新日期:2016-08-01 00:00:00

  • Retinal oxygen saturation as a non-invasive estimate for mixed venous oxygen saturation and cardiac output.

    abstract:PURPOSE:To investigate the correlation between retinal vessel oxygen saturation and mixed venous oxygen saturation (SvO2-mixed ) and cardiac output (CO). METHODS:Retinal arterial (SaO2-retinal ) and venous (SvO2-retinal ) oxygen saturation were measured non-invasively with dual-wavelength retinal oximetry in subjects ...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.13890

    authors: Van Keer K,Van Keer J,Barbosa Breda J,Nassiri V,Van Cleemput J,Abegão Pinto L,Stalmans I,Vandewalle E

    更新日期:2019-03-01 00:00:00

  • Clinical risk management - a 3-year experience of team timeout in 18 081 ophthalmic patients.

    abstract:PURPOSE:Clinical risk management aims to identify, analyse and avoid errors and risks systematically to improve patient's safety. Preoperative checklists to prevent mistakes have gained importance in the last few years. A so-called team timeout checklist was introduced in October 2011 at the Department of Ophthalmology...

    journal_title:Acta ophthalmologica

    pub_type: 杂志文章

    doi:10.1111/aos.13155

    authors: Weingessel B,Haas M,Vécsei C,Vécsei-Marlovits PV

    更新日期:2017-03-01 00:00:00