Bacterial community associated to the pine wilt disease insect vectors Monochamus galloprovincialis and Monochamus alternatus.

Abstract:

:Monochamus beetles are the dispersing vectors of the nematode Bursaphelenchus xylophilus, the causative agent of pine wilt disease (PWD). PWD inflicts significant damages in Eurasian pine forests. Symbiotic microorganisms have a large influence in insect survival. The aim of this study was to characterize the bacterial community associated to PWD vectors in Europe and East Asia using a culture-independent approach. Twenty-three Monochamus galloprovincialis were collected in Portugal (two different locations); twelve Monochamus alternatus were collected in Japan. DNA was extracted from the insects' tracheas for 16S rDNA analysis through denaturing gradient gel electrophoresis and barcoded pyrosequencing. Enterobacteriales, Pseudomonadales, Vibrionales and Oceanospirilales were present in all samples. Enterobacteriaceae was represented by 52.2% of the total number of reads. Twenty-three OTUs were present in all locations. Significant differences existed between the microbiomes of the two insect species while for M. galloprovincialis there were no significant differences between samples from different Portuguese locations. This study presents a detailed description of the bacterial community colonizing the Monochamus insects' tracheas. Several of the identified bacterial groups were described previously in association with pine trees and B. xylophilus, and their previously described functions suggest that they may play a relevant role in PWD.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Alves M,Pereira A,Matos P,Henriques J,Vicente C,Aikawa T,Hasegawa K,Nascimento F,Mota M,Correia A,Henriques I

doi

10.1038/srep23908

subject

Has Abstract

pub_date

2016-04-05 00:00:00

pages

23908

issn

2045-2322

pii

srep23908

journal_volume

6

pub_type

杂志文章
  • Seed germination of Caragana species from different regions is strongly driven by environmental cues and not phylogenetic signals.

    abstract::Seed germination behavior is an important factor in the distribution of species. Many studies have shown that germination is controlled by phylogenetic constraints, however, it is not clear whether phylogenetic constraints or environmental cues explain seed germination of a genus from a common ancestor. In this study,...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-11294-x

    authors: Fang XW,Zhang JJ,Xu DH,Pang J,Gao TP,Zhang CH,Li FM,Turner NC

    更新日期:2017-09-12 00:00:00

  • Intermittent Lactobacilli-containing Vaginal Probiotic or Metronidazole Use to Prevent Bacterial Vaginosis Recurrence: A Pilot Study Incorporating Microscopy and Sequencing.

    abstract::Bacterial vaginosis (BV) is associated with HIV acquisition and adverse pregnancy outcomes. Recurrence after metronidazole treatment is high. HIV-negative, non-pregnant Rwandan BV patients were randomized to four groups (n = 17/group) after seven-day oral metronidazole treatment: behavioral counseling only (control), ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60671-6

    authors: van de Wijgert JHHM,Verwijs MC,Agaba SK,Bronowski C,Mwambarangwe L,Uwineza M,Lievens E,Nivoliez A,Ravel J,Darby AC

    更新日期:2020-03-03 00:00:00

  • Lactoferrin-derived Peptides Active towards Influenza: Identification of Three Potent Tetrapeptide Inhibitors.

    abstract::Bovine lactoferrin is a biglobular multifunctional iron binding glycoprotein that plays an important role in innate immunity against infections. We have previously demonstrated that selected peptides from bovine lactoferrin C-lobe are able to prevent both Influenza virus hemagglutination and cell infection. To deeper ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-10492-x

    authors: Scala MC,Sala M,Pietrantoni A,Spensiero A,Di Micco S,Agamennone M,Bertamino A,Novellino E,Bifulco G,Gomez-Monterrey IM,Superti F,Campiglia P

    更新日期:2017-09-06 00:00:00

  • Cd(II) Sorption on Montmorillonite-Humic acid-Bacteria Composites.

    abstract::Soil components (e.g., clays, bacteria and humic substances) are known to produce mineral-organic composites in natural systems. Herein, batch sorption isotherms, isothermal titration calorimetry (ITC), and Cd K-edge EXAFS spectroscopy were applied to investigate the binding characteristics of Cd on montmorillonite(Mo...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19499

    authors: Du H,Chen W,Cai P,Rong X,Dai K,Peacock CL,Huang Q

    更新日期:2016-01-21 00:00:00

  • Loss of lamin B1 is a biomarker to quantify cellular senescence in photoaged skin.

    abstract::Skin ageing is an inevitable consequence of life and accelerated by exposure to ultraviolet (UV) rays. Senescence is an irreversible growth arrest and senescent cells accumulate in ageing tissues, at sites of age-related pathologies and in pre-neoplastic lesions. Conventionally, senescent cells have been detected by s...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-15901-9

    authors: Wang AS,Ong PF,Chojnowski A,Clavel C,Dreesen O

    更新日期:2017-11-15 00:00:00

  • Magnetically-induced ferroelectricity in the (ND4)2[FeCl5(D2O)] molecular compound.

    abstract::The number of magnetoelectric multiferroic materials reported to date is scarce, as magnetic structures that break inversion symmetry and induce an improper ferroelectric polarization typically arise through subtle competition between different magnetic interactions. The (NH4)2[FeCl5(H2O)] compound is a rare case wher...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep14475

    authors: Alberto Rodríguez-Velamazán J,Fabelo Ó,Millán Á,Campo J,Johnson RD,Chapon L

    更新日期:2015-09-29 00:00:00

  • PTPRJ Inhibits Leptin Signaling, and Induction of PTPRJ in the Hypothalamus Is a Cause of the Development of Leptin Resistance.

    abstract::Leptin signaling in the hypothalamus plays a crucial role in the regulation of body weight. Leptin resistance, in which leptin signaling is disrupted, is a major obstacle to the improvement of obesity. We herein demonstrated that protein tyrosine phosphatase receptor type J (Ptprj) is expressed in hypothalamic neurons...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-12070-7

    authors: Shintani T,Higashi S,Suzuki R,Takeuchi Y,Ikaga R,Yamazaki T,Kobayashi K,Noda M

    更新日期:2017-09-14 00:00:00

  • Phylogeny and expression pattern analysis of TCP transcription factors in cassava seedlings exposed to cold and/or drought stress.

    abstract::The TCP transcription factors usually act as integrators of multiple growth regulatory and environmental stimuli. However, little is known about this gene family in the important tropical crop cassava (Manihot esculenta). In this study, 36 TCP genes were identified and renamed based on cassava whole-genome sequence an...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-09398-5

    authors: Lei N,Yu X,Li S,Zeng C,Zou L,Liao W,Peng M

    更新日期:2017-08-30 00:00:00

  • Evaluation of Novel Targeted Therapies in Aggressive Biology Sarcoma Patients after progression from US FDA approved Therapies.

    abstract::Prognosis of patients with advanced sarcoma after progression from FDA approved therapies remains grim. In this study, clinical outcomes of 100 patients with advanced sarcoma who received treatment on novel targeted therapy trials were evaluated. Outcomes of interest included best response, clinical benefit rate, prog...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep35448

    authors: Subbiah V,Hess KR,Khawaja MR,Wagner MJ,Tang C,Naing A,Fu S,Janku F,Piha-Paul S,Tsimberidou AM,Herzog CE,Ludwig JA,Patel S,Ravi V,Benjamin RS,Meric-Bernstam F,Hong DS

    更新日期:2016-10-17 00:00:00

  • DPP-4 Inhibitor and Estrogen Share Similar Efficacy Against Cardiac Ischemic-Reperfusion Injury in Obese-Insulin Resistant and Estrogen-Deprived Female Rats.

    abstract::Estrogen deprivation aggravates cardiac injury after myocardial ischemia and reperfusion (I/R) injury. Although either estrogen or the dipeptidyl peptidase-4 (DPP-4) inhibitor, vildagliptin, reduces myocardial damage following cardiac I/R, their effects on the heart in obese-insulin resistant and estrogen deprived con...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep44306

    authors: Sivasinprasasn S,Tanajak P,Pongkan W,Pratchayasakul W,Chattipakorn SC,Chattipakorn N

    更新日期:2017-03-10 00:00:00

  • Surface chemistry for cytosolic gene delivery and photothermal transgene expression by gold nanorods.

    abstract::Light-inducible gene regulation has great potential for remote and noninvasive control of the fate and function of target cells. One method to achieve such control is delivery of heat shock protein (HSP) promoter-driven protein expression vectors and photothermal heaters into the cells, followed by activation by illum...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-04912-1

    authors: Nakatsuji H,Kawabata Galbraith K,Kurisu J,Imahori H,Murakami T,Kengaku M

    更新日期:2017-07-05 00:00:00

  • Nanofiber-expanded human CD34+ cells heal cutaneous wounds in streptozotocin-induced diabetic mice.

    abstract::Despite advances in diabetic wound care, the significant number of amputations that occur every year demands more effective therapeutics. Herein, we offer an aminated polyethersulfone nanofiber-expanded human umbilical cord blood-derived CD34+ cells (henceforth CD34+ cells) effective therapy, tested in cutaneous wound...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-44932-7

    authors: Kanji S,Das M,Joseph M,Aggarwal R,Sharma SM,Ostrowski M,Pompili VJ,Mao HQ,Das H

    更新日期:2019-06-10 00:00:00

  • The CD63-Syntenin-1 Complex Controls Post-Endocytic Trafficking of Oncogenic Human Papillomaviruses.

    abstract::Human papillomaviruses enter host cells via a clathrin-independent endocytic pathway involving tetraspanin proteins. However, post-endocytic trafficking required for virus capsid disassembly remains unclear. Here we demonstrate that the early trafficking pathway of internalised HPV particles involves tetraspanin CD63,...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep32337

    authors: Gräßel L,Fast LA,Scheffer KD,Boukhallouk F,Spoden GA,Tenzer S,Boller K,Bago R,Rajesh S,Overduin M,Berditchevski F,Florin L

    更新日期:2016-08-31 00:00:00

  • Genetic variability of the U5 and downstream sequence of major HIV-1 subtypes and circulating recombinant forms.

    abstract::The critical role of the regulatory elements at the 5' end of the HIV-1 genome in controlling the life cycle of HIV-1 indicates that this region significantly influences virus fitness and its biological properties. In this study, we performed a detailed characterization of strain-specific variability of sequences from...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-70083-1

    authors: Mbondji-Wonje C,Dong M,Zhao J,Wang X,Nanfack A,Ragupathy V,Sanchez AM,Denny TN,Hewlett I

    更新日期:2020-08-06 00:00:00

  • The effects of exercise treatment on learning and memory ability, and cognitive performance in diet-induced prediabetes animals.

    abstract::Changes associated with cognitive function in the high-fat high-carbohydrate diet-induced prediabetes animal model and effect of exercise remain unclear. Rats were randomly assigned to the following groups (n = 6): non-diabetic (ND), prediabetic (PD), intermittent exercising PD (PD + IE) and regular exercising PD (PD ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-72098-0

    authors: Luvuno M,Khathi A,Mabandla MV

    更新日期:2020-09-14 00:00:00

  • Lipid yield from the diatom Porosira glacialis is determined by solvent choice and number of extractions, independent of cell disruption.

    abstract::Cell wall disruption is necessary to maximize lipid extraction yields in conventional species of mass-cultivated microalgae. This study investigated the effect of sonication, solvent choice and number of extractions on the lipid yield, lipid class composition and fatty acid composition of the diatom Porosira glacialis...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79269-z

    authors: Svenning JB,Dalheim L,Vasskog T,Matricon L,Vang B,Olsen RL

    更新日期:2020-12-17 00:00:00

  • Camouflage and Clutch Survival in Plovers and Terns.

    abstract::Animals achieve camouflage through a variety of mechanisms, of which background matching and disruptive coloration are likely the most common. Although many studies have investigated camouflage mechanisms using artificial stimuli and in lab experiments, less work has addressed camouflage in the wild. Here we examine e...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep32059

    authors: Stoddard MC,Kupán K,Eyster HN,Rojas-Abreu W,Cruz-López M,Serrano-Meneses MA,Küpper C

    更新日期:2016-09-12 00:00:00

  • Temporal dynamics of choice behavior in rats and humans: an examination of pre- and post-choice latencies.

    abstract::Identifying similarities and differences in choice behavior across species is informative about how basic mechanisms give rise to more complex processes. In the present study, we compared pre- and post-choice latencies between rats and humans under two paradigms. In Experiment 1, we used a cued choice paradigm where s...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep20583

    authors: Fam J,Westbrook F,Arabzadeh E

    更新日期:2016-02-10 00:00:00

  • Short-term Dynamics after Single- and Three-piece Acrylic Intraocular Lens Implantation: A Swept-source Anterior Segment Optical Coherence Tomography Study.

    abstract::Accurate alignment of an intraocular lens (IOL) is indispensable for achieving accurate postoperative refractive outcomes. Thus, we evaluated decentration and tilt of single- and three-piece IOLs, as well as anterior chamber depth (ACD), at 3 hours, 24 hours, 2 weeks, and 4 weeks after cataract surgery, using swept-so...

    journal_title:Scientific reports

    pub_type: 杂志文章,随机对照试验

    doi:10.1038/s41598-018-28609-1

    authors: Sato T,Shibata S,Yoshida M,Hayashi K

    更新日期:2018-07-06 00:00:00

  • Effect of adipose-derived mesenchymal stem cell transplantation on vascular calcification in rats with adenine-induced kidney disease.

    abstract::Previous studies have investigated the use of mesenchymal stem cells (MSCs) to treat damaged kidneys. However, the effect of adipose-derived MSCs (ASCs) on vascular calcification in chronic kidney disease (CKD) is still poorly understood. In the present study, we explored the potential of ASCs for the treatment of CKD...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-14492-9

    authors: Yokote S,Katsuoka Y,Yamada A,Ohkido I,Yokoo T

    更新日期:2017-10-25 00:00:00

  • Association of admission serum levels of vitamin D, calcium, Phosphate, magnesium and parathormone with clinical outcomes in neurosurgical ICU patients.

    abstract::To evaluate the association of admission serum levels of 25(OH)D, parathormone and the related electrolytes with severity of illness and clinical outcomes in neurosurgical critically ill patients, serum levels of 25(OH)D, parathormone, calcium, magnesium, and phosphate, along with APACHE II score were measured for 210...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21177-4

    authors: Ardehali SH,Dehghan S,Baghestani AR,Velayati A,Vahdat Shariatpanahi Z

    更新日期:2018-02-14 00:00:00

  • Ultralow-phase-noise millimetre-wave signal generator assisted with an electro-optics-modulator-based optical frequency comb.

    abstract::Low-noise millimetre-wave signals are valuable for digital sampling systems, arbitrary waveform generation for ultra-wideband communications, and coherent radar systems. However, the phase noise of widely used conventional signal generators (SGs) will increase as the millimetre-wave frequency increases. Our goal has b...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep24621

    authors: Ishizawa A,Nishikawa T,Goto T,Hitachi K,Sogawa T,Gotoh H

    更新日期:2016-05-17 00:00:00

  • Quantitative estimation of track segment yields of water radiolysis species under heavy ions around Bragg peak energies using Geant4-DNA.

    abstract::We evaluate the track segment yield G' of typical water radiolysis products (eaq-, ·OH and H2O2) under heavy ions (He, C and Fe ions) using a Monte Carlo simulation code in the Geant4-DNA. Furthermore, we reproduce experimental results of ·OH of He and C ions around the Bragg peak energies (< 6 MeV/u). In the relative...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-021-81215-6

    authors: Baba K,Kusumoto T,Okada S,Ogawara R,Kodaira S,Raffy Q,Barillon R,Ludwig N,Galindo C,Peaupardin P,Ishikawa M

    更新日期:2021-01-15 00:00:00

  • Biochemical evidence for relaxed substrate specificity of Nα-acetyltransferase (Rv3420c/rimI) of Mycobacterium tuberculosis.

    abstract::Nα-acetylation is a naturally occurring irreversible modification of N-termini of proteins catalyzed by Nα-acetyltransferases (NATs). Although present in all three domains of life, it is little understood in bacteria. The functional grouping of NATs into six types NatA - NatF, in eukaryotes is based on subunit require...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep28892

    authors: Pathak D,Bhat AH,Sapehia V,Rai J,Rao A

    更新日期:2016-06-29 00:00:00

  • A novel application of hematite precipitation for high effective separation of Fe from Nd-Fe-B scrap.

    abstract::Rare earths, e.g. neodymium (Nd), praseodymium (Pr) and dysprosium (Dy), are abundant in the rare earth sintered magnet scrap (Nd-Fe-B scrap), but their recycling is tedious and costly due to the high content of impurity Fe. Herein, a novel approach was developed to effectively recycle rare earths from the scrap via a...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-54896-3

    authors: Lin X,Qu Z,Chen Y,Jin R,Su T,Yu Y,Zhu S,Huo M,Peng J,Wang Z

    更新日期:2019-12-04 00:00:00

  • Effects of Killari earthquake on the paleo-channel of Tirna River Basin from Central India using anisotropy of magnetic susceptibility.

    abstract::The Killari Earthquake (Moment magnitude 6.1) of September 30, 1993, occurred in the state of Maharashtra, India, has an epicenter (18°03' N, 76°33' E) located at ~ 40 km SSW of Killari Town. The ~ 125 km long basin of Tirna River, close to the Killari Town, currently occupies the area that has witnessed episodic intr...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77542-9

    authors: Lakshmi BV,Deenadayalan K,Gawali PB,Misra S

    更新日期:2020-11-25 00:00:00

  • Key sources and seasonal dynamics of greenhouse gas fluxes from yak grazing systems on the Qinghai-Tibetan Plateau.

    abstract::Greenhouse gas (GHG) emissions from livestock grazing systems are contributing to global warming. To examine the influence of yak grazing systems on GHG fluxes and relationships between GHG fluxes and environmental factors, we measured carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O) fluxes over three key s...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40857

    authors: Liu Y,Yan C,Matthew C,Wood B,Hou F

    更新日期:2017-01-20 00:00:00

  • Efficacy and safety of GLP-1 receptor agonists as add-on to SGLT2 inhibitors in type 2 diabetes mellitus: A meta-analysis.

    abstract::GLP-1 receptor agonists (GLP-1RA) and SGLT2 inhibitors (SGLT2i) have been associated with improved glycemic control, body weight loss and favorable changes in cardiovascular risk factors and outcomes. We conducted a systematic review and meta-analysis to evaluate the effects of the addition of GLP-1RA to SGLT2i in pat...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/s41598-019-55524-w

    authors: Castellana M,Cignarelli A,Brescia F,Perrini S,Natalicchio A,Laviola L,Giorgino F

    更新日期:2019-12-18 00:00:00

  • In situ investigation of phase transformations in Ti-6Al-4V under additive manufacturing conditions combining laser melting and high-speed micro-X-ray diffraction.

    abstract::We present combined in situ X-ray diffraction and high-speed imaging to monitor the phase evolution upon cyclic rapid laser heating and cooling mimicking the direct energy deposition of Ti-6Al-4V in real time. Additive manufacturing of the industrially relevant alloy Ti-6Al-4V is known to create a multitude of phases ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-16760-0

    authors: Kenel C,Grolimund D,Li X,Panepucci E,Samson VA,Sanchez DF,Marone F,Leinenbach C

    更新日期:2017-11-27 00:00:00

  • Memorization of daily routines by children with Down syndrome assisted by a playful virtual environment.

    abstract::A child with Down syndrome, like any other child, may benefit from interacting with memory stimuli, but needs additional support and help. The use of special teaching methods, which add playfulness and use of the computer, can enhance the memory processes of these children. In this work, we present the virtual environ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-60014-5

    authors: da Cruz Netto OL,Rodrigues SCM,de Castro MV,da Silva DP,da Silva RR,de Souza RRB,de Souza AAF,Bissaco MAS

    更新日期:2020-02-21 00:00:00