Abstract:
AIM:To investigate Fusobacterium nucleatum (F. nucleatum) abundance in colorectal cancer (CRC) tissues and its association with CRC invasiveness in Chinese patients. METHODS:The resected cancer and adjacent normal tissues (10 cm beyond cancer margins) from 101 consecutive patients with CRC were collected. Fluorescent quantitative polymerase chain reaction (FQ-PCR) was applied to detect F. nucleatum in CRC and normal tissues. The difference of F. nucleatum abundance between cancer and normal tissues and the relationship of F. nucleatum abundance with clinical variables were evaluated. Fluorescence in situ hybridization (FISH) analysis was performed on 22 CRC tissues with the highest F. nucleatum abundance by FQ-PCR testing to confirm FQ-PCR results. RESULTS:The median abundance of F. nucleatum in CRC tissues [0.242 (0.178-0.276)] was significantly higher than that in normal controls [0.050 (0.023-0.067)] (P < 0.001). F. nucleatum was over-represented in 88/101 (87.1%) CRC samples. The abundance of F. nucleatum determined by 2(-ΔCT) was significantly greater in tumor samples [0.242 (0.178, 0.276)] than in normal controls [0.050 (0.023, 0.067)] (P < 0.001). The frequency of patients with lymph node metastases was higher in the over-abundance group [52/88 (59.1%)] than in the under-abundance group [0/13 (0%)] (P < 0.005). No significant association of F. nucleatum with other clinico-pathological variables was observed (P > 0.05). FISH analysis also found more F. nucleatum in CRC than in normal tissues (median number 6, 25(th) 3, 75(th) 10 vs 2, 25(th) 1, 75(th) 5) (P < 0.01). CONCLUSION:F. nucleatum was enriched in CRC tissues and associated with CRC development and metastasis.
journal_name
World J Gastroenteroljournal_title
World journal of gastroenterologyauthors
Li YY,Ge QX,Cao J,Zhou YJ,Du YL,Shen B,Wan YJ,Nie YQdoi
10.3748/wjg.v22.i11.3227subject
Has Abstractpub_date
2016-03-21 00:00:00pages
3227-33issue
11eissn
1007-9327issn
2219-2840journal_volume
22pub_type
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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pub_type: 杂志文章,meta分析,评审
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章,meta分析,评审
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journal_title:World journal of gastroenterology
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
doi:10.3748/wjg.v16.i18.2235
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pub_type: 杂志文章
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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pub_type: 社论,评审
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journal_title:World journal of gastroenterology
pub_type: 杂志文章,评审
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更新日期:2004-01-01 00:00:00
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章
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journal_title:World journal of gastroenterology
pub_type: 杂志文章
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更新日期:2005-06-28 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2013-12-07 00:00:00