Abstract:
:We have characterized a member of the profilin (PROF) family protein as a common antigen in three pathogens-Babesia bovis (B. bovis), Babesia bigemina (B. bigemina), and Babesia microti (B. microti)-and evaluated its immunogenic and cross-protective properties against a challenge infection with B. microti in BALB/c mice. The recombinant PROF proteins of B. bovis, B. bigemina, and B. microti were successfully expressed in Escherichia coli (E. coli) as soluble GST fusion proteins (rBboPROF, rBbigPROF, and rBmPROF, respectively), and they were found to be antigenic. On probing with mouse anti-rPROF serum, green fluorescence was observed on the parasites' cytosols by confocal laser microscopy. Immunization regimes in BALB/c mice using rPROFs induced cross-protective immunity against B. microti infection based on high levels of cytokines and immunoglobulin (IgG) titers, a reduction in peak parasitemia levels, and earlier clearance of the parasite as compared with control mice. The findings of the present study indicate that PROF is a common antigen among bovine and murine Babesia parasites, and it might be used as a common vaccine candidate against babesiosis.
journal_name
Exp Parasitoljournal_title
Experimental parasitologyauthors
Munkhjargal T,Aboge GO,Ueno A,Aboulaila M,Yokoyama N,Igarashi Idoi
10.1016/j.exppara.2016.03.024subject
Has Abstractpub_date
2016-07-01 00:00:00pages
29-36eissn
0014-4894issn
1090-2449pii
S0014-4894(16)30050-9journal_volume
166pub_type
杂志文章abstract::Murine malarial parasites have long been characterized by their requirement for either antibody-mediated immunity (AMI) or cell-mediated immunity (CMI) for suppression of acute parasitemia, with Plasmodium yoelii reportedly requiring AMI for suppression and P. chabaudi requiring CMI. To assess this characterization in...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.4132
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章,评审
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abstract::Angiostrongylus cantonensis causes eosinophilic meningitis and eosinophilic pleocytosis in humans and is of significant socio-economic importance globally. microRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in gene expression regulation, cellular function and defense, homeostasis and patho...
journal_title:Experimental parasitology
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2008.03.006
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1994.1014
更新日期:1994-03-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(88)90116-6
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2016.12.013
更新日期:2017-01-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/s0014-4894(03)00096-1
更新日期:2003-03-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2009.10.011
更新日期:2010-09-01 00:00:00
abstract::Two strains of mice which share identical H-2 genes but differ in their genetic backgrounds were compared for their ability to resist infection with Trichinella spiralis. The two strains of mice, C3HeB/FeJ and AKR/J, share the H-2k haplotype which is associated with susceptibility to primary infection with T. spiralis...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(83)90009-7
更新日期:1983-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.06.002
更新日期:2012-10-01 00:00:00
abstract::The effect of infection by Angiostrongylus cantonensis on the activity of the enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the concentration of total proteins, uric acid and urea in the hemolymph of Biomphalaria glabrata were investigated. The snails were dissected after 1, 2 and 3 w...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.03.003
更新日期:2012-06-01 00:00:00
abstract::The effects of azadirachtin and ecdysone on the Trypanosoma cruzi population in the Rhodnius prolixus gut were investigated. T. cruzi were rarely found in the gut compartments of azadirachtin-treated larvae. High parasite numbers were observed in the stomach of the control and ecdysone groups until 10 days after treat...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2012.05.005
更新日期:2012-07-01 00:00:00
abstract::Several species of the genus Leishmania are causative agents of cutaneous leishmaniasis in Pakistan. This study aimed to determine phylogenetic placement of Leishmania species causing cutaneous leishmaniasis in Khyber Pakhtunkhwa province, Pakistan (34 Leishmania tropica, 3 Leishmania infantum), in-relation to species...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2016.05.006
更新日期:2016-08-01 00:00:00
abstract::The analysis of PvuII restriction patterns of Leishmania spp. and Trypanosoma spp. genomic DNA showed genus distinctive profiles. A specific PvuII site was detected in the 5' domain of 18S ribosomal DNA of Leishmania. A 20-mer oligonucleotide encompassing this PvuII region was synthesized. This sequence, when utilized...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(91)90133-h
更新日期:1991-02-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2006.09.019
更新日期:2007-04-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2008.04.013
更新日期:2008-08-01 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2011.10.011
更新日期:2012-02-01 00:00:00
abstract::Immunity in mice infected with Toxoplasma gondii is dependent upon the ability to generate protective levels of the cytokine IFN-gamma. In this report, we present evidence that the attenuated vaccine strain, ts-4, induces the latter cytokine by both IL-12-dependent and -independent pathways. In contrast, strain ME49 a...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1996.0096
更新日期:1996-11-01 00:00:00
abstract::One of the most fascinating aspects of the Entamoeba histolytica trophozoite ultrastructure is the lack of a typical secretory pathway, particularly of rough endoplasmic reticulum and Golgi system, in a cell with such a high secretory activity. Here, we describe the isolation of amoeba cell structures containing ER-ty...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2005.04.002
更新日期:2005-08-01 00:00:00
abstract::Chryseobacterium indologenes and Chryseobacterium gleum are Gram negative environmental bacteria that have been frequently reported to implicate in fatal nosocomial infections, such as bacteraemia and ventilator-associated pneumonia in immunocompromised individuals in the past decades. The interaction between Chryseob...
journal_title:Experimental parasitology
pub_type: 杂志文章
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更新日期:2020-02-19 00:00:00
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journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/0014-4894(86)90130-x
更新日期:1986-02-01 00:00:00
abstract::Amphotericin B (Amp) has been well-successfully used to treat against Leishmania infection, although high toxicity has been found in patients. In the present study, Amp was administered in Leishmania infantum-infected BALB/c mice by three distinct delivery systems aiming to compare their efficacy against challenge inf...
journal_title:Experimental parasitology
pub_type: 杂志文章
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abstract::A Real-time quantitative PCR assay to quantify the Toxoplasma gondii apicoplast was studied. Primers were designed to amplify a 305bp product specific to T. gondii apicoplast. Standard curves were generated for both T. gondii apicoplast DNA and genomic DNA, and were used to compute the relative concentration of apicop...
journal_title:Experimental parasitology
pub_type: 杂志文章
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abstract::High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were inf...
journal_title:Experimental parasitology
pub_type: 杂志文章
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abstract::The aim of this study was to observe the intestinal mucosal/systemic responses triggered by intranasal vaccination using recombinant Trichinella spiralis serine protease (rTsSP) and its capacity to elicit immune protection against larva challenge in a murine model. rTsSP coupled with cholera toxin B subunit (CTB) was ...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2019.04.006
更新日期:2019-06-01 00:00:00
abstract::There is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. We are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. We used an in vivo bioassay for sporozoite invasion of salivary glands. In this assay,...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1006/expr.1995.1124
更新日期:1995-11-01 00:00:00
abstract::Most of the drugs used for the treatment of trichinellosis show a limited bioavailability and a high degree of resistance. Therefore, this study aimed to characterize the anthelmintic potential activity of nitazoxanide (NTZ) in a rat model of experimental trichinellosis. Animals were divided into three groups; group I...
journal_title:Experimental parasitology
pub_type: 杂志文章
doi:10.1016/j.exppara.2016.08.009
更新日期:2016-11-01 00:00:00