Identification and characterization of profilin antigen among Babesia species as a common vaccine candidate against babesiosis.

Abstract:

:We have characterized a member of the profilin (PROF) family protein as a common antigen in three pathogens-Babesia bovis (B. bovis), Babesia bigemina (B. bigemina), and Babesia microti (B. microti)-and evaluated its immunogenic and cross-protective properties against a challenge infection with B. microti in BALB/c mice. The recombinant PROF proteins of B. bovis, B. bigemina, and B. microti were successfully expressed in Escherichia coli (E. coli) as soluble GST fusion proteins (rBboPROF, rBbigPROF, and rBmPROF, respectively), and they were found to be antigenic. On probing with mouse anti-rPROF serum, green fluorescence was observed on the parasites' cytosols by confocal laser microscopy. Immunization regimes in BALB/c mice using rPROFs induced cross-protective immunity against B. microti infection based on high levels of cytokines and immunoglobulin (IgG) titers, a reduction in peak parasitemia levels, and earlier clearance of the parasite as compared with control mice. The findings of the present study indicate that PROF is a common antigen among bovine and murine Babesia parasites, and it might be used as a common vaccine candidate against babesiosis.

journal_name

Exp Parasitol

authors

Munkhjargal T,Aboge GO,Ueno A,Aboulaila M,Yokoyama N,Igarashi I

doi

10.1016/j.exppara.2016.03.024

subject

Has Abstract

pub_date

2016-07-01 00:00:00

pages

29-36

eissn

0014-4894

issn

1090-2449

pii

S0014-4894(16)30050-9

journal_volume

166

pub_type

杂志文章
  • The time course of selected malarial infections in cytokine-deficient mice.

    abstract::Murine malarial parasites have long been characterized by their requirement for either antibody-mediated immunity (AMI) or cell-mediated immunity (CMI) for suppression of acute parasitemia, with Plasmodium yoelii reportedly requiring AMI for suppression and P. chabaudi requiring CMI. To assess this characterization in...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1996.4132

    authors: van der Heyde HC,Pepper B,Batchelder J,Cigel F,Weidanz WP

    更新日期:1997-02-01 00:00:00

  • Selective killing of Leishmania amastigotes expressing a thymidine kinase suicide gene.

    abstract::The thymidine kinase gene of Herpes simplex type-1 virus was transfected into several Leishmania species to create drug-sensitive mutants. Expression of the thymidine kinase gene is not by itself harmful to Leishmania cells but it is capable of phosphorylating ganciclovir, a nucleoside analog, into a highly toxic prod...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1996.4115

    authors: Muyombwe A,Olivier M,Ouellette M,Papadopoulou B

    更新日期:1997-01-01 00:00:00

  • Effects of Microtus fortis lymphocytes on Schistosoma japonicum in a bone marrow transplantation model.

    abstract::Microtus fortis is a non-permissive host for Schistosoma japonicum. While M. fortis lymphocytes are known to provide natural resistance against S. japonicum, the specific mechanism remains unclear. A bone marrow transplantation (BMT) model was established using immunodeficient mice, either nude (experiment 1) or V(D)J...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2014.04.005

    authors: Hu Y,Xu Y,Lu W,Quan H,Shen Y,Yuan Z,Zhang J,Zang W,He Y,Cao J

    更新日期:2014-07-01 00:00:00

  • Emodepside and SL0-1 potassium channels: a review.

    abstract::Nematode parasites infect humans and domestic animals; treatment and prophylaxis require anthelmintic drugs because vaccination and sanitation is limited. Emodepside is a more recently introduced cyclooctadepsipeptide drug that has actions against GI nematodes, lungworm, and microfilaria. It has a novel mode of action...

    journal_title:Experimental parasitology

    pub_type: 杂志文章,评审

    doi:10.1016/j.exppara.2011.08.012

    authors: Martin RJ,Buxton SK,Neveu C,Charvet CL,Robertson AP

    更新日期:2012-09-01 00:00:00

  • Angiostrongylus cantonensis: identification and characterization of microRNAs in male and female adults.

    abstract::Angiostrongylus cantonensis causes eosinophilic meningitis and eosinophilic pleocytosis in humans and is of significant socio-economic importance globally. microRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in gene expression regulation, cellular function and defense, homeostasis and patho...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2011.02.019

    authors: Chen MX,Ai L,Xu MJ,Zhang RL,Chen SH,Zhang YN,Guo J,Cai YC,Tian LG,Zhang LL,Zhu XQ,Chen JX

    更新日期:2011-06-01 00:00:00

  • An improved method to obtain antigen-excreting Toxocara canis larvae.

    abstract::Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) when infecting humans as a larva. The infection is demonstrated by detecting IgG antibodies against excretory-secretory larval antigens (ESLA) in serum by ELISA. The production of ESLA involves the collection of adult worms from dog puppy stool...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2008.03.006

    authors: Alcântara-Neves NM,dos Santos AB,Mendonça LR,Figueiredo CA,Pontes-de-Carvalho L

    更新日期:2008-07-01 00:00:00

  • Vaccination of sheep with purified cysteine proteinases of Fasciola hepatica decreases worm fecundity.

    abstract::There has been some evidence from several parasite systems that proteinases might have potential as protective antigens against parasitic infection. A cysteine proteinase complex identified in the regurgitant of adult F. hepatica was examined in this context. The thiol-cathepsin-related proteinases of M(r) 28,000 were...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1994.1014

    authors: Wijffels GL,Salvatore L,Dosen M,Waddington J,Wilson L,Thompson C,Campbell N,Sexton J,Wicker J,Bowen F

    更新日期:1994-03-01 00:00:00

  • Giardia lamblia: localization of hydrolase activities in lysosome-like organelles of trophozoites.

    abstract::Homogenates of Giardia lamblia trophozoites exhibited the following hydrolase activities: acid phosphatase (EC 3.1.3.2), proteinase (EC 3.1.4) with urea-denatured hemoglobin and N-benzoyl-DL-arginine-2-naphthylamide as substrates, deoxyribonuclease (EC 3.1.4.5), and ribonuclease (EC 2.7.7.16). beta-N-Acetylglucosamini...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(88)90116-6

    authors: Lindmark DG

    更新日期:1988-02-01 00:00:00

  • Antiprotozoal drug nitazoxanide enhances parasitemia, tissue lesions and mortality caused by Trypanosoma cruzi in murine model.

    abstract::Chagas' disease is caused by unicellular parasite Trypanosoma cruzi (T. cruzi). It is endemic throughout Latin America, but nowadays has become a global challenge due to tourism and migration. Non-treated infection may result in health-threatening complications and lead to death. Current medications for this infection...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2016.12.013

    authors: Valle-Reyes JS,Melnikov V,Dobrovinskaya O,Rodriguez-Hernández A,Wookee-Zea C,Pimientel-Rodrigez V,Rueda-Valdovinos G,Delgado-Enciso I,López-Lemus UA,Espinoza-Gómez F

    更新日期:2017-01-01 00:00:00

  • Trypanosoma cruzi reinfections provoke synergistic effect and cardiac beta-adrenergic receptors' dysfunction in the acute phase of experimental Chagas' disease.

    abstract::Cardiac beta-adrenergic receptors' function was studied in the acute phase of Chagas' disease in mice reinfected with Trypanosoma cruzi Tulahuen strain (Tul) and with parasites isolated from an infected patient (SGO-Z12). Genetic characterization of SGO-Z12 isolates demonstrated that it belongs to the zimodeme Z12, on...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/s0014-4894(03)00096-1

    authors: Bustamante JM,Rivarola HW,Fernández AR,Enders JE,Ricardo F,d'Oro Gloria DL,Palma JA,Paglini-Oliva PA

    更新日期:2003-03-01 00:00:00

  • Acanthamoeba spp.: in vitro effects of clinical isolates on murine macrophages, osteosarcoma and HeLa cells.

    abstract::Three different cell lines (murine macrophages, HeLa and osteosarcoma cells) were assayed in order to check for the manifestation of the cytopathic effects of three strains of Acanthamoeba recently isolated in our laboratory from contact lens cases: CLC-16, CLC-41.r and CLC-51-l. Adhesion and cytotoxicity assays were ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2009.10.011

    authors: Martín-Navarro CM,Lorenzo-Morales J,Machín RP,López-Arencibia A,Valladares B,Piñero JE

    更新日期:2010-09-01 00:00:00

  • Trichinella spiralis: role of non-H-2 genes in resistance to primary infection in mice.

    abstract::Two strains of mice which share identical H-2 genes but differ in their genetic backgrounds were compared for their ability to resist infection with Trichinella spiralis. The two strains of mice, C3HeB/FeJ and AKR/J, share the H-2k haplotype which is associated with susceptibility to primary infection with T. spiralis...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(83)90009-7

    authors: Wassom DL,Brooks BO,Cypess RH,David CS

    更新日期:1983-04-01 00:00:00

  • Cell death features induced in Leishmania major by 1,3,4-thiadiazole derivatives.

    abstract::Under a variety of stress conditions, Leishmania species display some morphological and biochemical features characteristic of mammalian programmed cell death or necrosis. Nitroheteroaryl-1,3,4-thiadiazoles induce cell death in Leishmania major (L. major). Putative mechanisms of action of these compounds were investig...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2012.06.002

    authors: Ardestani SK,Poorrajab F,Razmi S,Foroumadi A,Ajdary S,Gharegozlou B,Behrouzi-Fardmoghadam M,Shafiee A

    更新日期:2012-10-01 00:00:00

  • Effects of infection by larvae of Angiostrongylus cantonensis (Nematoda, Metastrongylidae) on the metabolism of the experimental intermediate host Biomphalaria glabrata.

    abstract::The effect of infection by Angiostrongylus cantonensis on the activity of the enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and the concentration of total proteins, uric acid and urea in the hemolymph of Biomphalaria glabrata were investigated. The snails were dissected after 1, 2 and 3 w...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2012.03.003

    authors: Tunholi-Alves VM,Tunholi VM,Pinheiro J,Thiengo SC

    更新日期:2012-06-01 00:00:00

  • Trypanosoma cruzi: effects of azadirachtin and ecdysone on the dynamic development in Rhodnius prolixus larvae.

    abstract::The effects of azadirachtin and ecdysone on the Trypanosoma cruzi population in the Rhodnius prolixus gut were investigated. T. cruzi were rarely found in the gut compartments of azadirachtin-treated larvae. High parasite numbers were observed in the stomach of the control and ecdysone groups until 10 days after treat...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2012.05.005

    authors: Cortez MR,Provençano A,Silva CE,Mello CB,Zimmermann LT,Schaub GA,Garcia ES,Azambuja P,Gonzalez MS

    更新日期:2012-07-01 00:00:00

  • Phylogenetic position of Leishmania isolates from Khyber Pakhtunkhwa province of Pakistan.

    abstract::Several species of the genus Leishmania are causative agents of cutaneous leishmaniasis in Pakistan. This study aimed to determine phylogenetic placement of Leishmania species causing cutaneous leishmaniasis in Khyber Pakhtunkhwa province, Pakistan (34 Leishmania tropica, 3 Leishmania infantum), in-relation to species...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2016.05.006

    authors: Khan NH,Messenger LA,Wahid S,Sutherland CJ

    更新日期:2016-08-01 00:00:00

  • Leishmania: genus identification based on a specific sequence of the 18S ribosomal RNA sequence.

    abstract::The analysis of PvuII restriction patterns of Leishmania spp. and Trypanosoma spp. genomic DNA showed genus distinctive profiles. A specific PvuII site was detected in the 5' domain of 18S ribosomal DNA of Leishmania. A 20-mer oligonucleotide encompassing this PvuII region was synthesized. This sequence, when utilized...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(91)90133-h

    authors: Uliana SR,Affonso MH,Camargo EP,Floeter-Winter LM

    更新日期:1991-02-01 00:00:00

  • Entamoeba histolytica: an ecto-phosphatase activity regulated by oxidation-reduction reactions.

    abstract::In this work, an ecto-phosphatase activity of Entamoeba histolytica was characterized using intact cells. This activity presented the following biochemical characteristics: (i) it hydrolyzes p-NPP with V(max) of 8.00+/-0.22 nmol p-NP x h(-1) x 10(-5) cells and K(m) of 2.68+/-0.25 mM; (ii) it is inhibited by acid phosp...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2006.09.019

    authors: de Sá Pinheiro AA,Amazonas JN,de Souza Barros F,De Menezes LF,Batista EJ,Silva EF,De Souza W,Meyer-Fernandes JR

    更新日期:2007-04-01 00:00:00

  • Echinococcus granulosus: cellular territories and morphological regions in mature protoscoleces.

    abstract::Basic aspects of the generation, structure and function of Echinococcus granulosus protoscoleces are unknown. We review the work done on the structure and ultrastructure of the E. granulosus protoscolex and provide new data together with a comprehensive view of this form of the parasite. The surface, as observed by sc...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2008.04.013

    authors: Galindo M,Schadebrodt G,Galanti N

    更新日期:2008-08-01 00:00:00

  • Expression profile and subcellular localization of HslV, the proteasome related protease from Trypanosoma cruzi.

    abstract::Trypanosoma cruzi is a rare example of an eukaryote that has genes for two threonine proteases: HslVU complex and 20S proteasome. HslVU is an ATP-dependent protease consisting of two multimeric components: the HslU ATPase and the HslV peptidase. In this study, we expressed and obtained specific antibodies to HslU and ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2011.10.011

    authors: Barboza NR,Cardoso J,de Paula Lima CV,Soares MJ,Gradia DF,Hangai NS,Bahia MT,de Lana M,Krieger MA,Guerra de Sá R

    更新日期:2012-02-01 00:00:00

  • Toxoplasma gondii: evidence for interleukin-12-dependent and-independent pathways of interferon-gamma production induced by an attenuated parasite strain.

    abstract::Immunity in mice infected with Toxoplasma gondii is dependent upon the ability to generate protective levels of the cytokine IFN-gamma. In this report, we present evidence that the attenuated vaccine strain, ts-4, induces the latter cytokine by both IL-12-dependent and -independent pathways. In contrast, strain ME49 a...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1996.0096

    authors: Scharton-Kersten T,Caspar P,Sher A,Denkers EY

    更新日期:1996-11-01 00:00:00

  • Entamoeba histolytica: biochemical and molecular insights into the activities within microsomal fractions.

    abstract::One of the most fascinating aspects of the Entamoeba histolytica trophozoite ultrastructure is the lack of a typical secretory pathway, particularly of rough endoplasmic reticulum and Golgi system, in a cell with such a high secretory activity. Here, we describe the isolation of amoeba cell structures containing ER-ty...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2005.04.002

    authors: Salgado M,Villagómez-Castro JC,Rocha-Rodríguez R,Sabanero-López M,Ramos MA,Alagón A,López-Romero E,Sánchez-López R

    更新日期:2005-08-01 00:00:00

  • Chryseobacterium indologenes and Chryseobacterium gleum interact and multiply intracellularly in Acanthamoeba castellanii.

    abstract::Chryseobacterium indologenes and Chryseobacterium gleum are Gram negative environmental bacteria that have been frequently reported to implicate in fatal nosocomial infections, such as bacteraemia and ventilator-associated pneumonia in immunocompromised individuals in the past decades. The interaction between Chryseob...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2020.107862

    authors: Lim WG,Tong T,Chew J

    更新日期:2020-02-19 00:00:00

  • Brugia pahangi: stage-specific antigens on microfilariae detected by serum from infected jirds or by monoclonal antibodies.

    abstract::Experiments were carried out to determine whether there are stage-specific antigens on microfilariae of Brugia pahangi, using sera from Mongolian jirds infected with B. pahangi and monoclonal antibodies against microfilariae of B. pahangi. These studies showed that microfilariae have both stage-specific and nonspecifi...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/0014-4894(86)90130-x

    authors: Tomisato M,Nariuchi H,Ueno J,Nogami S,Tanaka H,Aoki Y,Nakajima Y

    更新日期:1986-02-01 00:00:00

  • Comparing the therapeutic efficacy of different amphotericin B-carrying delivery systems against visceral leishmaniasis.

    abstract::Amphotericin B (Amp) has been well-successfully used to treat against Leishmania infection, although high toxicity has been found in patients. In the present study, Amp was administered in Leishmania infantum-infected BALB/c mice by three distinct delivery systems aiming to compare their efficacy against challenge inf...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2018.02.003

    authors: Mendonça DVC,Martins VT,Lage DP,Dias DS,Ribeiro PAF,Carvalho AMRS,Dias ALT,Miyazaki CK,Menezes-Souza D,Roatt BM,Tavares CAP,Barichello JM,Duarte MC,Coelho EAF

    更新日期:2018-03-01 00:00:00

  • Toxoplasma gondii: a simple Real-time PCR assay to quantify the proliferation of the apicoplast.

    abstract::A Real-time quantitative PCR assay to quantify the Toxoplasma gondii apicoplast was studied. Primers were designed to amplify a 305bp product specific to T. gondii apicoplast. Standard curves were generated for both T. gondii apicoplast DNA and genomic DNA, and were used to compute the relative concentration of apicop...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2009.08.013

    authors: Wu L,Chen SX,Jiang XG,Cao JP

    更新日期:2009-12-01 00:00:00

  • Flow cytometric screening for anti-leishmanials in a human macrophage cell line.

    abstract::High-throughput drug screening methods against the intracellular stage of Leishmania have been facilitated by the development of in vitro models of infection. The use of cell lines rather than primary cells facilitates these methods. Peripheral blood mononuclear cell (PBMC) derived macrophages and THP-1 cells were inf...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2010.06.007

    authors: Mehta SR,Zhang XQ,Badaro R,Spina C,Day J,Chang KP,Schooley RT

    更新日期:2010-12-01 00:00:00

  • Intranasal immunization with recombinant Trichinella spiralis serine protease elicits protective immunity in BALB/c mice.

    abstract::The aim of this study was to observe the intestinal mucosal/systemic responses triggered by intranasal vaccination using recombinant Trichinella spiralis serine protease (rTsSP) and its capacity to elicit immune protection against larva challenge in a murine model. rTsSP coupled with cholera toxin B subunit (CTB) was ...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2019.04.006

    authors: Sun GG,Lei JJ,Ren HN,Zhang Y,Guo KX,Long SR,Liu RD,Jiang P,Wang ZQ,Cui J

    更新日期:2019-06-01 00:00:00

  • Plasmodium gallinaceum: sporozoite invasion of Aedes aegypti salivary glands is inhibited by anti-gland antibodies and by lectins.

    abstract::There is evidence which suggests that malaria sporozoites recognize mosquito salivary glands by specific receptor-ligand interactions. We are interested in identifying the putative salivary gland receptor(s) for sporozoite invasion. We used an in vivo bioassay for sporozoite invasion of salivary glands. In this assay,...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1006/expr.1995.1124

    authors: Barreau C,Touray M,Pimenta PF,Miller LH,Vernick KD

    更新日期:1995-11-01 00:00:00

  • Nitazoxanide anthelmintic activity against the enteral and parenteral phases of trichinellosis in experimentally infected rats.

    abstract::Most of the drugs used for the treatment of trichinellosis show a limited bioavailability and a high degree of resistance. Therefore, this study aimed to characterize the anthelmintic potential activity of nitazoxanide (NTZ) in a rat model of experimental trichinellosis. Animals were divided into three groups; group I...

    journal_title:Experimental parasitology

    pub_type: 杂志文章

    doi:10.1016/j.exppara.2016.08.009

    authors: Ashour DS,Abou Rayia DM,Saad AE,El-Bakary RH

    更新日期:2016-11-01 00:00:00