Substrate specificity of proteolytic activity in the testes fluid and seminal plasma of the common carp Cyprinus carpio.

Abstract:

:Substrate specificity in the seminal plasma and testes fluids of the common carp Cyprinus carpio was determined using gelatin, casein, albumin and haemoglobin. Proteolytic profiles of the testes and seminal plasma were compared. Different ranges of pH (5·5-9·5) and temperature (4-37° C) were used during incubations of seminal plasma proteinases. Differences in proteolytic activity between testes and seminal plasma may reflect specific functions of the testes and sperm ducts in semen production. Seminal plasma metalloproteinases were characterized by higher substrate specificity than were serine proteinases. Zymography optimization for seminal plasma indicated that pH 7·5 and 22° C were the optimal conditions for gel incubations.

journal_name

J Fish Biol

journal_title

Journal of fish biology

authors

Cejko BI,Słowińska M,Judycka S,Kowalski RK

doi

10.1111/jfb.12957

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

1904-17

issue

5

eissn

0022-1112

issn

1095-8649

journal_volume

88

pub_type

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