Abstract:
:Substrate specificity in the seminal plasma and testes fluids of the common carp Cyprinus carpio was determined using gelatin, casein, albumin and haemoglobin. Proteolytic profiles of the testes and seminal plasma were compared. Different ranges of pH (5·5-9·5) and temperature (4-37° C) were used during incubations of seminal plasma proteinases. Differences in proteolytic activity between testes and seminal plasma may reflect specific functions of the testes and sperm ducts in semen production. Seminal plasma metalloproteinases were characterized by higher substrate specificity than were serine proteinases. Zymography optimization for seminal plasma indicated that pH 7·5 and 22° C were the optimal conditions for gel incubations.
journal_name
J Fish Bioljournal_title
Journal of fish biologyauthors
Cejko BI,Słowińska M,Judycka S,Kowalski RKdoi
10.1111/jfb.12957subject
Has Abstractpub_date
2016-05-01 00:00:00pages
1904-17issue
5eissn
0022-1112issn
1095-8649journal_volume
88pub_type
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