Abstract:
:Several varieties of peptone supported growth of A. culbertsoni to different extents reaching a maximum cell density of 1-2 X 10(6)/ml. Proteose peptone and tryptone also yielded good growth when combined with thiamine and vitamin B12. A combination of proteose peptone with glucose, yeast extract and salts promoted excellent growth of A. culbertsoni with cell density reaching 1-2 X 10(7) cells/ml; tryptone and one of the indigenous peptones also yielded comparable growth when substituted for proteose peptone in this medium. Casamino acids also supported good growth of amoebae and requirement of yeast extract could be met by a combination of thiamine, vitamin B12 and biotin. Bacto peptone did not support good growth of this amoeba but supplementation of peptone with casamino acids or amino acid mixture improved the growth supporting capacity of the medium. Development of several media with or without glucose will aid in cultivation of A. culbertsoni, studies on its metabolism as well as screening of potential drugs.
journal_name
Indian J Exp Bioljournal_title
Indian journal of experimental biologyauthors
Shukla OP,Kaul SM,Mehlotra RKsubject
Has Abstractpub_date
1989-09-01 00:00:00pages
785-91issue
9eissn
0019-5189issn
0975-1009journal_volume
27pub_type
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
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更新日期:1996-09-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1989-05-01 00:00:00
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1992-08-01 00:00:00
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
doi:
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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journal_title:Indian journal of experimental biology
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更新日期:1997-01-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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journal_title:Indian journal of experimental biology
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更新日期:2001-01-01 00:00:00
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journal_title:Indian journal of experimental biology
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doi:
更新日期:2001-03-01 00:00:00
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journal_title:Indian journal of experimental biology
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更新日期:1994-09-01 00:00:00
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章
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journal_title:Indian journal of experimental biology
pub_type: 杂志文章,评审
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更新日期:2010-07-01 00:00:00
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journal_title:Indian journal of experimental biology
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doi:
更新日期:1989-08-01 00:00:00
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