Development of improved media for axenic cultivation of Acanthamoeba culbertsoni, Singh and Das 1970.

Abstract:

:Several varieties of peptone supported growth of A. culbertsoni to different extents reaching a maximum cell density of 1-2 X 10(6)/ml. Proteose peptone and tryptone also yielded good growth when combined with thiamine and vitamin B12. A combination of proteose peptone with glucose, yeast extract and salts promoted excellent growth of A. culbertsoni with cell density reaching 1-2 X 10(7) cells/ml; tryptone and one of the indigenous peptones also yielded comparable growth when substituted for proteose peptone in this medium. Casamino acids also supported good growth of amoebae and requirement of yeast extract could be met by a combination of thiamine, vitamin B12 and biotin. Bacto peptone did not support good growth of this amoeba but supplementation of peptone with casamino acids or amino acid mixture improved the growth supporting capacity of the medium. Development of several media with or without glucose will aid in cultivation of A. culbertsoni, studies on its metabolism as well as screening of potential drugs.

journal_name

Indian J Exp Biol

authors

Shukla OP,Kaul SM,Mehlotra RK

subject

Has Abstract

pub_date

1989-09-01 00:00:00

pages

785-91

issue

9

eissn

0019-5189

issn

0975-1009

journal_volume

27

pub_type

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