Abstract：
:As a major intracellular degradation and recycling machinery, autophagy plays an important role in maintaining cellular homeostasis and remodeling during normal development. Our previous study showed that fluorapatite (FA) crystal-coated electrospun polycaprolactone (PCL) was capable of inducing differentiation and mineralization of human dental pulp stem cells. However, how autophagy changes and whether autophagy plays a vital role during these processes is still unknown. In this study, we seeded STEMPRO human adipose-derived stem cells (ASCs) on both PCL+FA and PCL scaffolds to investigate the osteogenic inductive ability of FA crystals and we observed the autophagy changes of these cells. Scanning electron microscopy and fluorescence microscopy images, along with DNA quantitation, showed that both PCL+FA and PCL scaffolds could sustain ASC growth but only the PCL+FA scaffold could sustain cell mineralization. This was confirmed by alkaline phosphatase activity and Alizarin red and Von Kossa staining results. The autophagy RT2 Profiler polymerase chain reaction array analysis showed many autophagy-related genes changes during ASC differentiation. Western blot analysis indicated that several autophagy-related proteins fluctuated during the procedure. Among them, the microtubule-associated protein 1 light chain 3 (LC3)-II protein changes of the ASCs grown on the 2- or 3-dimensional environments at 6 h, 12 h, 1 d, 3 d, 7 d, 14 d, and 21 d reached a peak value at day 7 during osteogenesis. At earlier stages (from day 0 to day 3), the addition of autophagy inhibitors (3-mathyladenine, bafilomycin A1, and NH4Cl) attenuated the expression of osteogenic related markers (osteopontin, alkaline phosphatase activity, Alizarin red, and Von Kossa) compared with the control group. All data indicated that autophagy played an important role in ASC differentiation on the PCL+FA scaffold. Inhibition of autophagy before day 3 strongly inhibited osteogenic differentiation and mineralization of ASCs in the 3-dimensional model. This observation further elucidates the mechanism of autophagy in mesenchymal stem cell osteogenic differentiation.
journal_name
J Dent Resjournal_title
Journal of dental researchauthors
Li Y,Guo T,Zhang Z,Yao Y,Chang S,Nör JE,Clarkson BH,Ni L,Liu Jdoi
10.1177/0022034516636852subject
Has Abstractpub_date
2016-06-01 00:00:00pages
650 – 656issue
6eissn
0022-0345issn
1544-0591pii
0022034516636852journal_volume
95pub_type
杂志文章abstract：:Growing interest in the treatment and prevention of Molar/Incisor Hypomineralization (MIH) warrants investigation into the protein composition of hypomineralized enamel. Hypothesizing abnormality akin to amelogenesis imperfecta, we profiled proteins in hypomineralized enamel from human permanent first molars using a b...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034510375824
更新日期：2010-10-01 00:00:00
abstract：:The creatinine-adjusted urinary concentration of mercury in 73 schoolchildren with a mean age of 12 years was determined. In addition, the number of amalgam restorations and their size, prevalence of allergy, and days absent from school due to illness were recorded for each individual. A significant positive correlati...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345870660061701
更新日期：1987-06-01 00:00:00
abstract：:This study has identified and characterized class II (Ia) antigen-expressing cells in the normal rat incisor pulp by immunohistochemistry and flow cytometry. Two types of Ia-expressing cells occurred: one with a pronounced dendritic appearance located primarily in the periphery of the pulp, and one with morphological ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345880670100301
更新日期：1988-10-01 00:00:00
abstract：:Dendritic cells, such as Langerhans cells (LC), in different ectodermal compartments may have different functional capabilities. The present study was undertaken to compare oral Langerhans cells (LC) with those of the epidermis in terms of their ability to co-stimulate T-cells in vitro. A Mixed Epithelial Cell Lymphoc...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345990780030701
更新日期：1999-03-01 00:00:00
abstract：:The aim of this study was to test the potential of liposomes as drug carriers to the ulcerated oral mucosa. Radioactive triamcinolone acetonide palmitate (3H-TRMAp) was encapsulated in large multilamellar lipid vesicles and served as the test lotion. 3H-TRMAp in solution served as control. Forty-six hamsters were divi...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650090501
更新日期：1986-09-01 00:00:00
abstract：:Patients with restorations cemented with a reinforced zinc oxide-eugenol cement and a zinc phosphate cement were recalled after a four- to seven-year interval. No significant differences in clinical success were observed between restoration luted with the two cements. No significant difference in the incidence of cari...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345760550022201
更新日期：1976-03-01 00:00:00
abstract：:Caries increment is affected by sugar-sweetened beverage (SSB) consumption. Taxing SSBs could reduce sugar consumption and caries increment. The authors aimed to estimate the impact of a 20% SSB sales tax on caries increment and associated treatment costs (as well as the resulting tax revenue) in the context of German...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034516660278
更新日期：2016-11-01 00:00:00
abstract：:Cementum forms the interface between root dentin and periodontal ligament through which periodontal connective tissue is attached to root surfaces. We have examined how cementum components influence the biological activities of gingival fibroblasts. Cementum was harvested from freshly extracted human teeth and extract...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345870660082301
更新日期：1987-08-01 00:00:00
abstract：:The feasibility of immunizing monkeys with enzymes from oral streptococci in an attempt to reduce dental caries was investigated. Forty rhesus monkeys, Macaca mulatta, were used. Cariogenic streptococci, S mutans, were implanted into all the monkeys' mouths. There was no pathological effect resulting from immunization...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560123001
更新日期：1977-12-01 00:00:00
abstract：:The sensitizing potential of three periodontal dressing materials was evaluated by the guinea pig maximization test. The results indicated a difference in the sensitization rate of the three materials. ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345780570110801
更新日期：1978-11-01 00:00:00
abstract：:To understand the role of betel quid (BQ) in the pathogenesis of oral submucous fibrosis (OSF) and oral cancer, we used DNA damage, cytotoxicity, and cell proliferation assays to study the pathobiological effects of aqueous extracts of three BQ constituents [betel nut (Areca catechu, BN), inflorescence of Piper betle ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345940730050501
更新日期：1994-05-01 00:00:00
abstract：:Three dental amalgams were investigated to determine if their abrasion resistance was significantly affected by different packing pressures. Abrasive wear tests were performed on a machine designed to test materials under conditions similar to masticatory function. The results show that the abrasion resistance of the ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560060901
更新日期：1977-06-01 00:00:00
abstract：:Mixtures containing two bacterial species were analyzed using flow cytometric techniques. Light scattering characteristics of Streptococcus mutans and Actinomyces viscosus show unique profiles for pure cultures. However, the light scatter analysis of a mixture containing these two species demonstrates overlapping near...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345840630080501
更新日期：1984-08-01 00:00:00
abstract：:A quantitative microprobe analysis was conducted on three commercial amalgams to determine the nature of the phases present. The presence of tin in the Ag-Hg phase (gamma1) was confirmed and Cu-3Sn was found in the original alloy particles. In the dispersant amalgam studied, little Sn-Hg phase (gamma-2) was found and ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：
更新日期：1975-03-01 00:00:00
abstract：:Impacted human third molar teeth were examined, both with the light and electron microscope, to determine the extent of the odontoblast process. The odontoblast process was found to be limited to the inner third of coronal dentin and inner half of mid-root dentin. ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/002203457905800412011
更新日期：1979-11-01 00:00:00
abstract：:Genetic influences are important in the determination of mandibular morphology, and growth hormone receptor (GHR) is believed to have an important influence on the growth of craniofacial bone. In this study, we used quantitative trait locus methods to evaluate the relationship between craniofacial morphology and singl...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/154405910508401116
更新日期：2005-11-01 00:00:00
abstract：:Three single-composition, high-copper amalgams were analyzed for the composition of their before- and after-reaction phases. The method used was electron probe microanalysis. The results may be summarized as follows: All alloys consist essentially of Ag3Sn (gamma), Cu3Sn (epsilon), and, in some cases, Ag4Sn (beta). Wh...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345840630062301
更新日期：1984-06-01 00:00:00
abstract：:Although the tripeptides Glu-O-Phosphoserine-Tyr and Glu-O-Phosphoserine-Leu have been identified in embryonic bovine enamel proteins, 1, 2 the issue of whether both sequences occur in each of the phosphopeptides, or whether certain sequences occur in specific peptides only, has recently been resolved by isolating hom...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345790580023101
更新日期：1979-03-01 00:00:00
abstract：:With the objective of contributing to the understanding of factors influencing the characteristics of dentition, dental observations from 429 children and adult highland people of western Bolivia are described. Various age, sex, ethnic, and village differences are observed in malocclusion, dental caries, periodontal d...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345780570030901
更新日期：1978-03-01 00:00:00
abstract：:Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) nanocomplexes incorporated into sugar-free chewing gum have been shown to remineralize enamel subsurface lesions in situ. The aim of this study was to compare the ability of CPP-ACP, with that of other forms of calcium, to be retained in supragingival plaque ...
journal_title：Journal of dental research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1177/154405910308200311
更新日期：2003-03-01 00:00:00
abstract：:With clinical oral examinations not always possible in health surveys, researchers may instead be invited to add questions to a wider health survey. In such situations, an item is needed which adequately represents both clinical and self-reported oral health. This study investigated the clinical validity of Locker's g...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034512460676
更新日期：2012-11-01 00:00:00
abstract：:The relationship between periodontitis and systemic bone mineral density in Japanese women is undetermined. We tested the hypothesis that periodontitis was more frequent in women with low metacarpal bone mineral density (m-BMD). Subjects were 190 Japanese women (89 premenopausal, 101 post-menopausal). Periodontal stat...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345010800090901
更新日期：2001-09-01 00:00:00
abstract：:Eugenol, in the 100 percent concentration in commercial use, was applied to a circumscribed area, 3 mm in diameter, of rat labial mucosa for 1 minute. Reaction periods of 15 minutes, 1, 2, 4 and 6 hours were then permitted. Using routine histological procedures for processing the experimental tissues, it was observed ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345780570110901
更新日期：1978-11-01 00:00:00
abstract：:The biochemical function, adenylate energy charge (AEC), correlates with the viable count of S. mutans. AEC may be used to estimate the percent viable fraction of bacteria in dental plaque samples. An interactive computer program designed to process the AEC data is described. ...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/002203457905800410011
更新日期：1979-11-01 00:00:00
abstract：:Gingiva from alloxan and streptozotocin-diabetic rats exhibited markedly enhanced collagenolytic activity in tissue culture. This effect was eliminated by puromycin or by repeated freeze-thawing of the tissue prior to incubation. Soluble extracts of the diabetic gingiva in situ were found to contain breakdown products...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345780570032101
更新日期：1978-03-01 00:00:00
abstract：:Selected area reflection electron diffraction has been used to determine the phases present in the reaction zones surrounding the Ag-Cu eutectic dispersant in Dispersalloy amalgam. The experiments were carried out on two kinds of specimens, (1) Dispersalloy amalgam and (2) Dispersalloy amalgam using 30, 50, 75, and 10...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345770560090101
更新日期：1977-09-01 00:00:00
abstract：:Biological evaluation procedures for the acceptance of new dental materials include testing for mucous membrane irritation and inflammation, with the hamster cheek pouch used as a model system. Previous reports have indicated both a low 14-day implant retention rate and poor differentiation between positive and negati...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345860650111801
更新日期：1986-11-01 00:00:00
abstract：:The composition of the degradable glasses used in commercial dental glass-ionomer cements determines their leaching behavior and hence the properties of the cement. The objective of the present study was to assess if the composition and leaching in acetic acid solutions are reflected in the x-ray diffraction character...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/00220345010800081601
更新日期：2001-08-01 00:00:00
abstract：:Medication-related osteonecrosis of the jaw (MRONJ), although initially believed to be exclusively associated with bisphosphonates, has been implicated in recent reports with additional drugs, especially the bone antiresorptive denosumab. The pathophysiology has not been fully elucidated, and no causal association bet...
journal_title：Journal of dental research
pub_type： 杂志文章,评审
doi：10.1177/0022034515572021
更新日期：2015-04-01 00:00:00
abstract：:The cariostatic benefit from water fluoridation is indisputable, but the knowledge of possible adverse effects on bone and fracture risk due to fluoride exposure is ambiguous. The association between long-term (chronic) drinking water fluoride exposure and hip fracture (ICD-7-9: '820' and ICD-10: 'S72.0-S72.2') was as...
journal_title：Journal of dental research
pub_type： 杂志文章
doi：10.1177/0022034513506443
更新日期：2013-11-01 00:00:00
