Abstract:
:This study aimed to evaluate the effects of supplementing freezing and thawing media with hyaluronic acid (HA) on the quality parameters of frozen-thawed boar spermatozoa. Boar semen samples were collected from seven mature Yorkshire boars once a week using the gloved hand technique; these samples were frozen-thawed in the extender with added HA. Boar sperm was cryopreserved in the extender with HA added at concentrations of 0 (used as control), 4, 6, 8, 8 and 12mg/L, and their effects on the quality of frozen-thawed boar sperm were evaluated. HA addition to the extender significantly improved sperm motility, sperm membrane integrity, mitochondrial activity, acrosomal integrity, superoxide dismutase and catalase activity, but decreased sperm malondialdehyde level (p<0.05). Therefore, HA could be a promising cryoprotectant for boar sperm.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Qian L,Yu S,Zhou Ydoi
10.1016/j.ijbiomac.2016.02.075subject
Has Abstractpub_date
2016-06-01 00:00:00pages
287-9eissn
0141-8130issn
1879-0003pii
S0141-8130(16)30217-3journal_volume
87pub_type
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