Topographic Cues Reveal Two Distinct Spreading Mechanisms in Blood Platelets.

Abstract:

:Blood platelets are instrumental in blood clotting and are thus heavily involved in early wound closure. After adhering to a substrate they spread by forming protrusions like lamellipodia and filopodia. However, the interaction of these protrusions with the physical environment of platelets while spreading is not fully understood. Here we dynamically image platelets during this spreading process and compare their behavior on smooth and on structured substrates. In particular we analyze the temporal evolution of the spread area, the cell morphology and the dynamics of individual filopodia. Interestingly, the topographic cues enable us to distinguish two spreading mechanisms, one that is based on numerous persistent filopodia and one that rather involves lamellipodia. Filopodia-driven spreading coincides with a strong response of platelet morphology to the substrate topography during spreading, whereas lamellipodia-driven spreading does not. Thus, we quantify different degrees of filopodia formation in platelets and the influence of filopodia in spreading on structured substrates.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Sandmann R,Köster S

doi

10.1038/srep22357

subject

Has Abstract

pub_date

2016-03-03 00:00:00

pages

22357

issn

2045-2322

pii

srep22357

journal_volume

6

pub_type

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