Quantitative analysis of dietary iron utilization for erythropoiesis in response to body iron status.

Abstract:

:Erythropoiesis requires large amounts of iron for hemoglobin synthesis. There are two sources of iron for erythropoiesis, dietary and stored iron; however, their relative contributions to erythropoiesis remain unknown. In this study, we used the stable iron isotope (57)Fe to quantify synthesis of hemoglobin derived from dietary iron. Using this method, we investigated the activities of dietary iron absorption and the utilization of dietary iron for erythropoiesis in responses to stimulated erythropoiesis and to interventions to alter body iron status. Under iron-loaded conditions, the activity of dietary iron absorption was clearly lowered in response to up-regulation of hepcidin, although the estimated activity of iron release from stored iron was not compared with that under control conditions. This result was supported by the observation that two duodenal iron transporters, divalent metal transporter 1 (DMT1) and ferroportin, were downregulated by iron loading, although the levels of expression of ferroportin in iron storage tissues were not changed by iron loading under erythropoietic stimulation by epoetin-β pegol (C.E.R.A., a long-acting erythropoiesis-stimulating agent). These results indicate that the dietary iron absorption system is more sensitive to body iron status than are reticuloendothelial iron- release mechanisms. Our data indicated that there could be a regulatory mechanism favoring use of stored iron over dietary iron under iron-loaded conditions.

journal_name

Exp Hematol

journal_title

Experimental hematology

authors

Matsuo-Tezuka Y,Noguchi-Sasaki M,Kurasawa M,Yorozu K,Shimonaka Y

doi

10.1016/j.exphem.2016.02.005

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

491-501

issue

6

eissn

0301-472X

issn

1873-2399

pii

S0301-472X(16)00062-X

journal_volume

44

pub_type

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