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The role of in-vivo and in-vitro maturation time on ooplasmic dysmaturity.

Abstract：

:This study investigates whether the timing of in-vivo and in-vitro maturation influences ooplasmic dysmaturity. This is a retrospective comparison of intracytoplasmic sperm injection (ICSI) cycles (index cycles) complicated by complete fertilization failure (CFF) to cycles with successful fertilization in the same patient. The cycle following the index cycle was modified intentionally to increase fertilization. The times between human chorionic gonadotrophin (HCG) trigger and oocyte retrieval, HCG trigger and removal of cumulus cells, and HCG trigger and sperm injection were recorded. Fifteen patients were included. Compared with successful fertilization cycles, index (CFF) cycles showed a shorter time interval between HCG trigger and oocyte retrieval (2029.0 ± 16 versus 2195.0 ± 10 min; P < 0.001), HCG trigger and removal of cumulus cells (2201.4 ± 15 versus 2309.0 ± 23 min; P < 0.001) and oocyte retrieval and removal of cumulus cells (114.0 ± 13 versus 171.8 ± 15 min; P < 0.001). The interval between HCG trigger and ICSI was comparable between groups. Findings reveal novel patterns in time intervals between HCG trigger, oocyte retrieval, removal of cumulus cells and ICSI. Thus, modulating time intervals between HCG trigger, oocyte retrieval, removal of cumulus cells and ICSI to grant fertilization seems feasible.

journal_name

Reprod Biomed Online

journal_title

Reproductive biomedicine online

authors

Pereira N,Neri QV,Lekovich JP,Palermo GD,Rosenwaks Z

doi

10.1016/j.rbmo.2016.01.007

subject

Has Abstract

pub_date

2016-04-01 00:00:00

pages

401-6

issue

4

eissn

1472-6483

issn

1472-6491

pii

S1472-6483(16)00043-2

journal_volume

32

pub_type

杂志文章

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