Abstract：
:Conditional genome engineering in the human malaria pathogen Plasmodium falciparum remains highly challenging. Here we describe a strategy for facile and rapid functional analysis of genes using an approach based on the Cre/lox system and tailored for organisms with short and few introns. Our method allows the conditional, site-specific removal of genomic sequences of essential and non-essential genes by placing loxP sites into a short synthetic intron to produce a module (loxPint) can be placed anywhere in open reading frames without compromising protein expression. When duplicated, the loxPint module serves as an intragenic recombineering point that can be used for the fusion of gene elements to reporters or the conditional introduction of point mutations. We demonstrate the robustness and versatility of the system by targeting the P. falciparum merozoite surface protein 1 gene (msp1), which has previously proven refractory to genetic interrogation, and the parasite exported kinase FIKK10.1.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Jones ML,Das S,Belda H,Collins CR,Blackman MJ,Treeck Mdoi
10.1038/srep21800subject
Has Abstractpub_date
2016-02-19 00:00:00pages
21800issn
2045-2322pii
srep21800journal_volume
6pub_type
杂志文章abstract：:We aimed to predict molecular subtypes of breast cancer using radiomics signatures extracted from synthetic mammography reconstructed from digital breast tomosynthesis (DBT). A total of 365 patients with invasive breast cancer with three different molecular subtypes (luminal A + B, luminal; HER2-positive, HER2; triple...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78681-9
更新日期：2020-12-09 00:00:00
abstract：:The high density nucleation of α-Al2O3 nanocrystallites was observed by rapid heating of the aluminum formate hydroxide-based precursor powder at 1200 °C for 50 s. The nucleation of α-Al2O3 nanocrystallites with less 10 nm in size from high purity aluminum oxide matrix has not been observed to our knowledge. Based on ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51156-2
更新日期：2019-10-17 00:00:00
abstract：:CD8+ T cells directed against beta cell autoantigens are considered relevant for the pathogenesis of type 1 diabetes. Using single cell T cell receptor sequencing of CD8+ T cells specific for the IGRP265-273 epitope, we examined whether there was expansion of clonotypes and sharing of T cell receptor chains in autorea...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep44661
更新日期：2017-03-16 00:00:00
abstract：:L-amino acid oxidases from snake venoms have been described to possess various biological functions. In this study, we investigated the inflammatory responses induced in vivo and in vitro by CR-LAAO, an L-amino acid oxidase isolated from Calloselasma rhodostoma venom, and its antitumor potential. CR-LAAO induced acute...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep42673
更新日期：2017-02-16 00:00:00
abstract：:Metastable ω phase is common in body-centred cubic (bcc) metals and alloys, including high-alloying steels. Recent theoretical calculations also suggest that the ω structure may act as an intermediate phase for face-centred cubic (fcc)-to-bcc transformation. Thus far, the role of the ω phase played in fcc-bcc martensi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15331
更新日期：2015-10-27 00:00:00
abstract：:Competing endogenous RNA (ceRNA) have received wide attention because they are a novel way to regulate genes through sharing microRNAs (miRNAs) that are crucial for complex processes in many diseases. However, no systematic analysis of ceRNA mechanism in cardiovascular disease (CVD) is known. To gain insights into the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10547-z
更新日期：2017-08-31 00:00:00
abstract：:Despite increasing work detailing the presence of foraging specializations across a range of taxa, limited attention so far has been given to the role of spatiotemporal variation in food predictability in shaping individual resource selection. Here, we studied the exploitation of human-provided carrion resources diffe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33564-y
更新日期：2018-10-11 00:00:00
abstract：:Bloodstain Pattern Analysis is a forensic discipline in which, among others, the position of victims can be determined at crime scenes on which blood has been shed. To determine where the blood source was investigators use a straight-line approximation for the trajectory, ignoring effects of gravity and drag and thus ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11461
更新日期：2015-06-22 00:00:00
abstract：:Unlike the constant nature of elastic coefficients of isotropic bulk materials, the Young's (E) and shear (μ) moduli of nano-structured (NS) gyroid metamaterials change with relative density (ρ), but at different rates depending on the cell size of the structure. These elastic behaviors displayed by E and μ cause cros...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10978-8
更新日期：2017-09-05 00:00:00
abstract：:Environmental stimuli have the ability to generate specific representations of the rewards they predict and in so doing alter the selection and performance of reward-seeking actions. The basolateral amygdala participates in this process, but precisely how is unknown. To rectify this, we monitored, in near-real time, b...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep12511
更新日期：2015-07-27 00:00:00
abstract：:In order to clearly understand the intrinsic microwave absorption properties of carbon nanomaterials, we proposed an efficient strategy to synthesize high purity metal-free carbon nanotubes (CNTs) over water-soluble K2CO3 particles through chemical vapor decomposition and water-washing process. The comparison results ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28310
更新日期：2016-06-21 00:00:00
abstract：:Cortical spreading depolarisation (CSD), the neural mechanism underlying migraine aura, may cause headache by sensitising the trigeminal system. Photophobia, the most bothersome accompanying symptom during migraine attacks, is more prevalent in migraine with aura than in migraine without aura. Whether CSD plays a role...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67948-w
更新日期：2020-07-10 00:00:00
abstract：:Vaginal microbiota is an important early source of bacterial colonization for newborns. However, only a few small studies have investigated the composition of vaginal microbiota during labor. In this work, we analyzed vaginal swabs collected at 36 weeks gestation and at the onset of labor from 256 women participating ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-17972-0
更新日期：2017-12-14 00:00:00
abstract：:The magnetic skyrmion is a nanoscale topological object characterized by the winding of magnetic moments, appearing in magnetic materials with broken inversion symmetry. Because of its low current threshold for driving the skyrmion motion, they have been intensely studied toward novel storage applications by using ele...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54833-4
更新日期：2019-12-05 00:00:00
abstract：:This study evaluated the antibacterial effects of short-time exposure of surface pre-reacted glass-ionomer (S-PRG) eluate on oral microcosm biofilm. Biofilms were treated with an S-PRG eluate at different concentrations (25%, 50%, and 100%), distilled water (DW), and 0.1% chlorhexidine (CHX) twice a day for 5 min repe...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-71363-6
更新日期：2020-09-02 00:00:00
abstract：:Neurologic complications associated with viral encephalitis, including seizures and cognitive impairment, are a global health issue, especially in children. We previously showed that hippocampal injury during acute picornavirus infection in mice is associated with calpain activation and is the result of neuronal death...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep28699
更新日期：2016-06-27 00:00:00
abstract：:Gas-filled hollow-core fiber (HCF) pulse post-compressors generating few- to single-cycle pulses are a key enabling tool for attosecond science and ultrafast spectroscopy. Achieving optimum performance in this regime can be extremely challenging due to the ultra-broad bandwidth of the pulses and the need of an adequat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20580-1
更新日期：2018-02-02 00:00:00
abstract：:Increased ultraviolet B (UVB) radiation due to global change can affect plant growth and metabolism. Here, we evaluated the capacity of quinoa to resist under short acute UVB irradiation. Quinoa was daily exposed for 30 or 60 min to 1.69 W m-2 UVB. The results showed that 30 min exposure in 9 d-course did not cause se...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-18710-2
更新日期：2018-01-10 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-019-54236-5
更新日期：2019-11-22 00:00:00
abstract：:Jellyfish locomotion and orientation have been studied in the past both in the laboratory, testing mostly small jellyfish, and in the field, where it was impossible to control the seawater currents. Utilizing an outdoor water flume, we tested the locomotion of jellyfish when swimming against and with currents of up to...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-56311-3
更新日期：2019-12-30 00:00:00
abstract：:The indirect interactions between insect vectors, such as whiteflies, and the viruses they transmit, such as begomoviruses, via host plants may produce a range of outcome depending on the species/strain of each of the three organisms involved, and the mechanisms underlying the variations are not well understood. Here,...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00692-w
更新日期：2017-04-03 00:00:00
abstract：:Surface ocean bubble-bursting generates aerosols composed of microscopic salt-water droplets, enriched in marine organic matter. The organic fraction profoundly influences aerosols' properties, by scattering solar radiations and nucleating water particles. Still little is known on the biochemical and microbiological c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10563-z
更新日期：2017-09-13 00:00:00
abstract：:Because of the small particle number of intracellular species participating in genetic circuits, stochastic fluctuations are inevitable. This intracellular noise is detrimental to precise regulation. To maintain the proper function of a cell, some natural motifs attenuate the noise at the protein level. In many biolog...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-39611-6
更新日期：2019-03-04 00:00:00
abstract：:This study was performed to evaluate clinical characteristics of branch retinal vein occlusion (BRVO) patients with increased retinal haemorrhage during macular oedema (MO) treatment. Patients were divided into increased and non-increased retinal haemorrhage groups. The former group was sub-divided based on the degree...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67395-7
更新日期：2020-06-24 00:00:00
abstract：:Fungi play a key role in the functioning of soil in terrestrial ecosystems, and in particular in the remediation of degraded soils. The contribution of fungi to carbon and nutrient cycles, along with their capability to mobilise soil trace elements, is well-known. However, the importance of life history strategy for t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-63194-2
更新日期：2020-04-15 00:00:00
abstract：:Intravitreal anti-vascular endothelial growth factor (VEGF) agents have revolutionized the treatment of retinopathy of prematurity (ROP); however, there are concerns regarding the potential systemic complications caused by those treatments. This study aimed to determine the serum concentrations of cytokines in infants...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69684-7
更新日期：2020-07-29 00:00:00
abstract：:MicroRNAs (miRNAs) are important effectors in mediating host-pathogen interaction. In this report, coelomocytes miRNA libraries of three Japanese sea cucumbers Apostichopus japonicus were built by Illumina(®) Hiseq2000 from different time points after lipopolysaccharide challenge (at time 0 h, 6 h and 12 h). The clean...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep17763
更新日期：2015-12-04 00:00:00
abstract：:An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-020-63944-2
更新日期：2020-04-22 00:00:00
abstract：:In mosquitoes, precise and efficient finding of a host animal is crucial for survival. One of the poorly understood aspects of mosquito blood-feeding behavior is how these insects target an optimal site in order to penetrate the skin and blood vessels without alerting the host animal. Here we provide new findings that...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep13444
更新日期：2015-08-26 00:00:00
abstract：:Multi-drug resistant efflux transporters found in Blood-Brain Barrier (BBB) acts as a functional barrier, by pumping out most of the drugs into the blood. Previous studies showed focused ultrasound (FUS) induced microbubble oscillation can disrupt the BBB by loosening the tight junctions in the brain endothelial cells...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep31201
更新日期：2016-08-11 00:00:00
