Abstract:
:The Escherichia coli Min system self-organizes into a cell-pole to cell-pole oscillator on the membrane to prevent divisions at the cell poles. Reconstituting the Min system on a lipid bilayer has contributed to elucidating the oscillatory mechanism. However, previous in vitro patterns were attained with protein densities on the bilayer far in excess of those in vivo and failed to recapitulate the standing wave oscillations observed in vivo. Here we studied Min protein patterning at limiting MinD concentrations reflecting the in vivo conditions. We identified "burst" patterns--radially expanding and imploding binding zones of MinD, accompanied by a peripheral ring of MinE. Bursts share several features with the in vivo dynamics of the Min system including standing wave oscillations. Our data support a patterning mechanism whereby the MinD-to-MinE ratio on the membrane acts as a toggle switch: recruiting and stabilizing MinD on the membrane when the ratio is high and releasing MinD from the membrane when the ratio is low. Coupling this toggle switch behavior with MinD depletion from the cytoplasm drives a self-organized standing wave oscillator.
journal_name
Proc Natl Acad Sci U S Aauthors
Vecchiarelli AG,Li M,Mizuuchi M,Hwang LC,Seol Y,Neuman KC,Mizuuchi Kdoi
10.1073/pnas.1600644113subject
Has Abstractpub_date
2016-03-15 00:00:00pages
E1479-88issue
11eissn
0027-8424issn
1091-6490pii
1600644113journal_volume
113pub_type
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