Abstract:
:This study presents an assay for the detection and quantification of soil metal superoxides and peroxides in regolith and soil. The O2 release (OR) assay is based on the enzymatic conversion of the hydrolysis products of metal oxides to O2 and their quantification by an O2 electrode based on the stoichiometry of the involved reactions. The intermediate product O₂˙⁻ from the hydrolysis of metal superoxides is converted by cytochrome c to O2 and by superoxide dismutase (SOD) to ½ mol O2 and ½ mol H2O2, which is then converted by catalase (CAT) to ½ mol O2. The product H2O2 from the hydrolysis of metal peroxides and hydroperoxides is converted to ½ mol O2 by CAT. The assay method was validated in a sealed sample chamber by using a liquid-phase Clark-type O2 electrode with known concentrations of O₂˙⁻ and H2O2, and commercial metal superoxide and peroxide mixed with Mars analog Mojave and Atacama Desert soils. Carbonates and perchlorates, both present on Mars, do not interfere with the assay. The assay lower limit of detection, when using luminescence quenching/optical sensing O2-electrodes, is 1 nmol O2 cm(-3) or better. The activity of the assay enzymes SOD and cytochrome c was unaffected up to 6 Gy exposure by γ radiation, while CAT retained 100% and 40% of its activity at 3 and 6 Gy, respectively, which demonstrates the suitability of these enzymes for planetary missions, for example, on Mars or Europa.
journal_name
Astrobiologyjournal_title
Astrobiologyauthors
Georgiou CD,Zisimopoulos D,Panagiotidis K,Grintzalis K,Papapostolou I,Quinn RC,McKay CP,Sun HJdoi
10.1089/ast.2015.1345subject
Has Abstractpub_date
2016-02-01 00:00:00pages
126-42issue
2eissn
1531-1074issn
1557-8070journal_volume
16pub_type
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