当前位置： SCI文献检索 > Jundishapur Journal of Microbiology期刊下所有文献 > Detection of Epstein-Barr Virus DNA in Langerhans Cell Histiocytosis.

Detection of Epstein-Barr Virus DNA in Langerhans Cell Histiocytosis.

Abstract：

BACKGROUND:Langerhans cell histiocytosis (LCH) is a rare histiocytic proliferation of unknown etiology. It is characterized by granuloma-like proliferation of Langerhans-type dendritic cells and mainly affects young children. Although multiple investigators have suggested the possible role of viruses, such as Epstein-Barr virus (EBV), human herpesvirus-6 (HHV-6), Herpes simplex virus (HSV) types 1 and 2, and Cytomegalovirus (CMV) in the pathogenesis of LCH, it remains, however, debated. OBJECTIVES:The EBV infection is reported to be associated with LCH. Nevertheless, no report could be found about involved Iranian children in English medical literature. In this study, we investigated the presence of EBV in Iranian children with LCH. PATIENTS AND METHODS:In this retrospective study, in which we investigated the prevalence of presence of EBV DNA in LCH, using paraffin-embedded tissue samples of 30 patients with LCH and 30 age and tissue-matched controls, who were operated for reasons other than infectious diseases (between the years 2002 and 2012), by real-time polymerase chain reaction (RT-PCR) method, in the department of pediatric pathology. No ethical issues arose in the study, because only the pathology reports were reviewed, retrospectively, and the patients were anonymous. RESULTS:There was a significant difference in prevalence of EBV presence between patients and controls. The EBV was found by RT-PCR in 19 (63.33%) out of 30 patients and only in eight (26.7%) of 30 control samples. The P = 0.004, was calculated using chi-square test (OR: 4.75; 95% CI: 1.58 ‒ 14.25). CONCLUSIONS:Our study is the first investigation performed on patients with LCH and its possible association with EBV in Iran. Considering the P = 0.004, which is statistically significant, the findings do support the hypothesis of a possible role for EBV in the pathogenesis of LCH. These results are in accordance with several previous investigations, with positive findings.

journal_name

Jundishapur J Microbiol

journal_title

Jundishapur journal of microbiology

authors

Khoddami M,Nadji SA,Dehghanian P,Vahdatinia M,Shamshiri AR

doi

10.5812/jjm.27219

subject

Has Abstract

pub_date

2015-12-26 00:00:00

pages

e27219

issue

eissn

2008-3645

issn

2008-4161

journal_volume

pub_type

杂志文章

在线工具

Detection of Epstein-Barr Virus DNA in Langerhans Cell Histiocytosis.