Detection and Typing of Human Papilloma Viruses by Nested Multiplex Polymerase Chain Reaction Assay in Cervical Cancer.

Abstract:

BACKGROUND:Cervical cancer is the leading cause of death from cancer in under-developed countries. Human papilloma virus (HPV) 16 and 18 are the most prevalent types associated with carcinogenesis in the cervix. Conventional Polymerase Chain Reaction (PCR), type-specific and consensus primer-based PCR followed by sequencing, Restriction Fragment Length Polymorphism (RFLP) or hybridization by specific probes are common methods for HPV detection and typing. In addition, some researchers have developed a multiplex PCR for simultaneous detection and typing of different HPVs. OBJECTIVES:The aim of the present study was to investigate the prevalence of HPV infection and its types in cervical Squamous Cell Carcinoma (SCC) using the Nested Multiplex PCR (NMPCR) assay. PATIENTS AND METHODS:Sixty-six samples with histologically confirmed SCC were evaluated. Total DNA was isolated by phenol-chloroform extraction and ethanol precipitation. Nested multiplex PCR was performed with first-round PCR by GP-E6/E7 consensus primers for amplification of the genomic DNA of all known mucosal HPV genotypes and second-round PCR by type-specific multiplex PCR primer cocktails. RESULTS:Human papilloma virus infection was detected in 78.8% of samples, with the highest prevalence of HPV 16 (60.6%) while concurrent infections with two types was detected in 10.6%. CONCLUSIONS:The NMPCR assay is more convenient and easy for analysis of results, which is important for fast diagnosis and patient management, in a type-specific manner.

authors

Jalal Kiani S,Shatizadeh Malekshahi S,Yousefi Ghalejoogh Z,Ghavvami N,Shafiei Jandaghi NZ,Shahsiah R,Jahanzad I,Yavarian J

doi

10.5812/jjm.26441

subject

Has Abstract

pub_date

2015-12-26 00:00:00

pages

e26441

issue

12

eissn

2008-3645

issn

2008-4161

journal_volume

8

pub_type

杂志文章
  • Yeast colonization and drug susceptibility pattern in the pediatric patients with neutropenia.

    abstract:BACKGROUND:Pediatric patients with neutropenia are vulnerable to invasive Candida infections. Candida is the primary cause of fungal infections, particularly in immunosuppressed patients. Candida albicans has been the most common etiologic agent of these infections, affecting 48% of patients. OBJECTIVES:The aim of thi...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.11858

    authors: Haddadi P,Zareifar S,Badiee P,Alborzi A,Mokhtari M,Zomorodian K,Pakshir K,Jafarian H

    更新日期:2014-09-01 00:00:00

  • In Vitro Evaluation of Enzymatic and Antifungal Activities of Soil-Actinomycetes Isolates and Their Molecular Identification by PCR.

    abstract:BACKGROUND:Human cutaneous infection caused by a homogeneous group of keratinophilic fungi called dermatophytes. These fungi are the most common infectious agents in humans that are free of any population and geographic area. Microsporum canis is a cause of dermatophytosis (Tinea) in recent years in Iran and atypical s...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.8(5)2015.14874

    authors: Keikha N,Ayatollahi Mousavi SA,Nakhaei AR,Yadegari MH,Shahidi Bonjar GH,Amiri S

    更新日期:2015-05-31 00:00:00

  • Medicinal Plants for the Treatment of Acne Vulgaris: A Review of Recent Evidences.

    abstract:CONTEXT:Acne vulgaris affects about 85% of teenagers and may continue to adulthood. There are about two million visits to physicians per year for teenagers and the direct cost of acne treatment in the US exceeds $1 billion per year. EVIDENCE ACQUISITION:A wide variety of treatment regimens exist for acne vulgaris incl...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章,评审

    doi:10.5812/jjm.25580

    authors: Nasri H,Bahmani M,Shahinfard N,Moradi Nafchi A,Saberianpour S,Rafieian Kopaei M

    更新日期:2015-11-21 00:00:00

  • Detection of KPC Carbapenemase in Pseudomonas aeruginosa Isolated From Clinical Samples Using Modified Hodge Test and Boronic Acid Phenotypic Methods and Their Comparison With the Polymerase Chain Reaction.

    abstract:BACKGROUND:Bacterial resistance to antibiotics has become a major source of concern for public health. Pseudomonas aeruginosa strains are important opportunistic pathogens. These bacteria have a high resistance to a wide range of existing antimicrobials and antibiotics. OBJECTIVES:The present study was performed to ev...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.27249

    authors: Falahat S,Shojapour M,Sadeghi A

    更新日期:2016-03-03 00:00:00

  • The cutaneous bacterial microflora of the bodybuilders using anabolic-androgenic steroids.

    abstract:BACKGROUND:Anabolic-androgenic steroids (AAS) abuse by the athletes has dramatically increased during the recent decades. These substances might increase the skin lipids and enhance the cutaneous microbial proliferation. OBJECTIVES:The current study aimed to investigate the potential side effects of AAS on the bacteri...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.12269

    authors: Zomorodian K,Rahimi MJ,Taheri M,Ghanbari Asad A,Khani S,Ahrari I,Pakshir K,Khashei R

    更新日期:2014-12-06 00:00:00

  • Comparison of Newly Assembled Full Length HIV-1 Integrase With Prototype Foamy Virus Integrase: Structure-Function Prospective.

    abstract:BACKGROUND:Drug design against human immunodeficiency virus type 1 (HIV-1) integrase through its mechanistic study is of great interest in the area in biological research. The main obstacle in this area is the absence of the full-length crystal structure for HIV-1 integrase to be used as a model. A complete structure, ...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.29773

    authors: Dayer MR

    更新日期:2016-02-15 00:00:00

  • Effects of Mycobacterium tuberculosis ESAT6-CFP10 Protein on Cell Viability and Production of Nitric Oxide in Alveolar Macrophages.

    abstract:BACKGROUND:Mycobacterium tuberculosis is the major pathogen of tuberculosis, which affects approximately one-third of the world's population. The 6 kDa early secreted antigenic target (ESAT6) and the 10 kDa culture filtrate protein (CFP10), which are secreted by the ESX-1 system in M. tuberculosis, can contribute to my...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.33264

    authors: Xie X,Han M,Zhang L,Liu L,Gu Z,Yang M,Yang H

    更新日期:2016-05-30 00:00:00

  • The association between oral lichen planus and hepatitis C virus infection; a report from northeast of iran.

    abstract:BACKGROUND:The association between hepatitis C virus (HCV) infection and oral lichen planus (OLP) has been the focus of many studies. Fifteen percent of HCV infections lead to sets of extrahepatic manifestations including lichen planus (LP). The prevalence of HCV is heavily influenced by geographical location. OBJECTI...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.8(4)2015.16741

    authors: Gerayli S,Meshkat Z,Pasdar A,Mosannen Mozafari P,Banihashemi E,Khajavi MA,Rasekhi J

    更新日期:2015-04-18 00:00:00

  • Antibiotics use patterns for surgical prophylaxis site infection in different surgical wards of a teaching hospital in ahvaz, iran.

    abstract:BACKGROUND:Despite the effectiveness of prophylactic antimicrobials to prevent surgical site infection the use of antibiotic prophylaxis is often inappropriate. OBJECTIVES:The current study aimed to determine the pattern of prophylactic antibiotic use in a teaching hospital affiliated to Jundishapur University of Medi...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.12251

    authors: Alavi SM,Roozbeh F,Behmanesh F,Alavi L

    更新日期:2014-11-01 00:00:00

  • Phylogenetic and Transcripts Profiling of Glucose Sensing Related Genes in Candida glabrata.

    abstract:BACKGROUND:The sensing mechanism of glucose in Saccharomyces cerevisiae is well studied. However, such information is scarcely found in other yeast species such as Candida glabrata. OBJECTIVES:This study aimed to identify the glucose sensing pathway related genes of C. glabrata and to analyze the regulation pattern of...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.25177

    authors: Ng TS,Mohd Desa MN,Sandai D,Chong PP,Than LT

    更新日期:2015-11-21 00:00:00

  • Detection of algD, oprL and exoA Genes by New Specific Primers as an Efficient, Rapid and Accurate Procedure for Direct Diagnosis of Pseudomonas aeruginosa Strains in Clinical Samples.

    abstract:BACKGROUND:Pseudomonas aeruginosa is an opportunistic pathogen that infects patients with cystic fibrosis, burning wounds, ophthalmic traumas and immunodeficiency. OBJECTIVES:The aim of the present study was to compare the efficiency of newly designed primer sets with some previously published primers for PCR detectio...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.13583

    authors: Rashno Taee S,Khansarinejad B,Abtahi H,Najafimosleh M,Ghaznavi-Rad E

    更新日期:2014-10-01 00:00:00

  • A Molecular Biological and Biochemical Investigation on Mycobacterium tuberculosis MutT Protein.

    abstract:BACKGROUND:Mycobacterium tuberculosis is a vicious microbe co-existing with the infected host. This pathogen exploited opportunities to spread during periods of urbanization and social upheaval, and got retreated with improved hygiene. OBJECTIVES:This investigation was designed to clone and characterize M. tuberculosi...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.9367

    authors: Huang HL,Su HT,Wu CH,Tsai-Wu JJ

    更新日期:2014-03-01 00:00:00

  • Anti-Listerial Activity of Four Seaweed Essential Oils Against Listeria monocytogenes.

    abstract:BACKGROUND:Listeria monocytogenes is one of the most virulent types of bacteria and causes severe foodborne illness, such as listeriosis. Because this pathogen has become resistant to sanitizers and other disinfectants that are used to clean utensils and surfaces during food processing, it poses a serious threat to the...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.31784

    authors: Patra JK,Baek KH

    更新日期:2016-06-28 00:00:00

  • Genetic elements associated with antimicrobial resistance among intestinal bacteria.

    abstract:BACKGROUND:Integrons are the major reasons of multidrug resistance (MDR) among enteropathogenic bacteria. Occurrence of horizontal gene transfer between integron-carrying microorganisms and other enteric bacteria may increase the rate of emergence of integron-associated antibiotic resistance. OBJECTIVES:The objective ...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.9924

    authors: Bakhshi B,Eftekhari N,Pourshafie MR

    更新日期:2014-05-01 00:00:00

  • Antimicrobial activity of seven essential oils from Iranian aromatic plants against common causes of oral infections.

    abstract:BACKGROUND:Over the past two decades, there has been a growing trend in using oral hygienic products originating from natural resources such as essential oils (EOs) and plant extracts. Seven aromatic plants used in this study are among popular traditional Iranian medicinal plants with potential application in modern me...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.17766

    authors: Zomorodian K,Ghadiri P,Saharkhiz MJ,Moein MR,Mehriar P,Bahrani F,Golzar T,Pakshir K,Fani MM

    更新日期:2015-02-19 00:00:00

  • New Insights into Sepsis Therapy Using Sepia Officinalis.

    abstract:BACKGROUND:Sepsis remains a major problem for both scientists and clinicians. Cecal ligation and puncture (CLP) is considered the gold standard for animal models of sepsis. The undesirable side effects of certain antibiotics have forced scientists to discover new, natural, and safe antimicrobial agents, such as cephalo...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.29331

    authors: Soliman AM,Fahmy SR,Sayed AA,Abd El-Latif AA

    更新日期:2016-01-02 00:00:00

  • Antibacterial Activity of Silver Nanoparticles and Their Combination with Zataria multiflora Essential Oil and Methanol Extract.

    abstract:BACKGROUND:Against a variety of antimicrobial resistant pathogens, the scientists attempted substitution of antimicrobial medicine with various nanoparticles and plant-based antibacterial substances. OBJECTIVES:The aim of this study was to assess the antibacterial effects of silver nanoparticles solely and in combinat...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.36070

    authors: Sheikholeslami S,Mousavi SE,Ahmadi Ashtiani HR,Hosseini Doust SR,Mahdi Rezayat S

    更新日期:2016-09-17 00:00:00

  • Cloning and Expression of Poly 3-Hydroxybutyrate Operon Into Escherichia coli.

    abstract:BACKGROUND:Poly 3-Hydroxybutyrate (PHB), a class of Poly Hydroxyalkanoates (PHAs), is a group of bacterial storage polymers, produced by various microorganisms in response to nutrient limitation. PHAs are biodegradable polymers which could be a good substitute for current petrochemical plastics. PHB has been synthesize...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.16318

    authors: Jari M,Khatami SR,Galehdari H,Shafiei M

    更新日期:2015-02-20 00:00:00

  • Emergence of SCCmec Type I Obtained From Clinical Samples in Shiraz Teaching Hospitals, South-West of Iran.

    abstract:BACKGROUND:Methicillin-resistant Staphylococcus aureus (MRSA) continues to be a major cause of nosocomial infections. Methicillin resistance in S. aureus is caused by the acquisition of the mecA gene, located on a mobile genetic element called the staphylococcal cassette chromosome (SCC). OBJECTIVES:The aim of this st...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.16998v2

    authors: Ebrahim-Saraie HS,Motamedifar M,Sarvari J,Hoseini Alfatemi SM

    更新日期:2015-06-27 00:00:00

  • Effect of Propranolol Alone and in Combination with Pyrimethamine on Acute Murine Toxoplasmosis.

    abstract:BACKGROUND:Toxoplasmosis is a public health problem worldwide. This complication principally affects immunodeficient patients and pregnant women. Toxoplasma gondii is an opportunistic parasite, causing severe illness among and death of high-risk individuals and treatment is becoming increasingly difficult owing to side...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.22572

    authors: Montazeri M,Daryani A,Ebrahimzadeh M,Ahmadpour E,Sharif M,Sarvi S

    更新日期:2015-09-07 00:00:00

  • Frequency of Borrelia in Morphea Lesion by Polymerase Chain Reaction in Northeast of Iran.

    abstract:BACKGROUND:The etiology of morphea is still unknown. Borrelia spp. as a causative agent of morphea has been discussed since 1985, but the relationship remains uncertain. OBJECTIVES:We aimed to find the frequency of Borrelia in morphea lesions by polymerase chain reaction (PCR) in northeast of Iran. PATIENTS AND METHO...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.19730v2

    authors: Yazdanpanah MJ,Sharifi N,Khooei A,Banihashemi M,Khaje-Daluee M,Shamsi A,Ghazvini K

    更新日期:2015-08-27 00:00:00

  • Isolation and Characterization of a Novel Magnetotactic Bacterium From Iran: Iron Uptake and Producing Magnetic Nanoparticles in Alphaproteobacterium MTB-KTN90.

    abstract:BACKGROUND:Magnetotactic bacteria (MTB) have the ability to biomineralize unique intracellular magnetic nanosize particles. These bacteria and their magnetosomes are under special attraction because of their great useful potential in nano-biotechnological and biomedical applications. MTB are ubiquitous in aquatic envir...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.19343

    authors: Tajer Mohammad Ghazvini P,Kermanshahi RK,Nozad Golikand A,Sadeghizadeh M

    更新日期:2014-09-01 00:00:00

  • The Prevalence Rate of Helicobacter pylori Infection in, Chronic Otitis Media With Effusion Patients.

    abstract:BACKGROUND:Otitis media with effusion (OME) is a common disease in children. Viral or bacterial infections, allergy, adenoids, functional abnormalities of the Eustachian tube, and gastroesophageal reflux might have a possible role in the pathogenesis of OME. However, the exact pathogenesis of OME is still unsettled. O...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.15694

    authors: Saki N,Samarbaf Zadeh AR,Sheikhpour Jonaky R,Noori SM,Kayedani GA,Nikakhlagh S

    更新日期:2014-03-01 00:00:00

  • Comparison of Serum Hepatitis B Virus DNA and HBsAg Levels Between HBeAg-Negative and HBeAg-Positive Chronic Hepatitis B Patients.

    abstract:BACKGROUND:Chronic hepatitis B consists of different clinical phases. Laboratory and histological assessments can help differentiate the clinical phases of this disease and thus lead to better management. OBJECTIVES:This study was conducted to determine laboratory and histological characteristics of HBeAg-negative and...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.21444

    authors: Keshvari M,Alavian SM,Sharafi H

    更新日期:2015-01-14 00:00:00

  • Expression Profiles of TGF-β and TLR Pathways in Porphyromonas gingivalis and Prevotella intermedia Challenged Osteoblasts.

    abstract:BACKGROUND:The presence of certain oral pathogens at implant sites can hinder the osseointegration process. However, it is unclear how and by what microorganisms it happens. OBJECTIVES:This study investigated whether the presence of oral pathogens of Porphyromonas gingivalis and Prevotella intermedia individually, pla...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.8(4)2015.17920

    authors: Aydin K,Ekinci FY,Korachi M

    更新日期:2015-04-18 00:00:00

  • Molecular Identification of Resistance Determinants, Integrons and Genetic Relatedness of Extensively Drug Resistant Acinetobacter baumannii Isolated From Hospitals in Tehran, Iran.

    abstract:BACKGROUND:Acinetobacter baumannii has emerged as an important nosocomial pathogen. Hospital outbreaks of extensively drug resistant (XDR) A. baumannii are a great concern. OBJECTIVES:Aims of this study were to characterize the resistance determinants and genetic relatedness of (XDR) A. baumannii isolates in hospitals...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.27021v2

    authors: Najar Peerayeh S,Karmostaji A

    更新日期:2015-07-25 00:00:00

  • Diagnostic Value of Serum Adenosine Deaminase (ADA) Level for Pulmonary Tuberculosis.

    abstract:BACKGROUND:Diagnosis of tuberculosis (TB) is not always easy, thus employing methods with a short duration and acceptable sensitivity and specificity is necessary to diagnose TB. OBJECTIVES:The aim of this study was to investigate the diagnostic value of serum adenosine deaminase (ADA) level for diagnosis of pulmonary...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.21760

    authors: Salmanzadeh S,Tavakkol H,Bavieh K,Alavi SM

    更新日期:2015-03-21 00:00:00

  • Bioassay Guided Fractionation of an Anti-Methicillin-Resistant Staphylococcus aureus Flavonoid From Bromus inermis Leyss Inflorescences.

    abstract:BACKGROUND:Plants are considered as promising sources of new antibacterial agents as well as bioassay guided fractionation. OBJECTIVES:In the present work, the antibacterial properties, especially against methicillin-resistant Staphylococcus aureus (MRSA), of Bromus inermis inflorescence was studied, using the bioassa...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.12739

    authors: Aliahmadi A,Mirzajani F,Ghassempour A,Sonboli A

    更新日期:2014-12-01 00:00:00

  • Antibiotic Susceptibility and mecA Frequency in Staphylococcus epidermidis, Isolated From Intensive Care Unit Patients.

    abstract:BACKGROUND:Coagulase-negative staphylococci (CoNS), especially Staphylococcus epidermidis, are considered as normal flora of human epithelia and also important opportunistic pathogens for nosocomial infections. S. epidermidis can also act as a reservoir for mecA, responsible for high-level resistance to methicillin and...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.11188

    authors: Najar-Peerayeh S,Jazayeri Moghadas A,Behmanesh M

    更新日期:2014-08-01 00:00:00

  • Evaluation of Gastric Lesions Based on Helicobacter pylori and Helicobacter-Like Organisms (HLOs) in Cats; A Histopathological and Bacteriological Study.

    abstract:BACKGROUND:The lesions induced by Helicobacter pylori in a candidate animal model should always be examined thoroughly. The resemblance of these lesions to those observed in humans can indicate whether the usage of this model will contribute to the understanding of the various pathogenic mechanisms involved in the deve...

    journal_title:Jundishapur journal of microbiology

    pub_type: 杂志文章

    doi:10.5812/jjm.9129

    authors: Sasani F,Javanbakht J,Kabir FR,Agha Mohammad Hassan M,Pashaei AR

    更新日期:2014-06-01 00:00:00