The Regulation of Exosporium-Related Genes in Bacillus thuringiensis.

Abstract:

:Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis (Bt) are spore-forming members of the Bacillus cereus group. Spores of B. cereus group species are encircled by exosporium, which is composed of an external hair-like nap and a paracrystalline basal layer. Despite the extensive studies on the structure of the exosporium-related proteins, little is known about the transcription and regulation of exosporium gene expression in the B. cereus group. Herein, we studied the regulation of several exosporium-related genes in Bt. A SigK consensus sequence is present upstream of genes encoding hair-like nap proteins (bclA and bclB), basal layer proteins (bxpA, bxpB, cotB, and exsY ), and inosine hydrolase (iunH). Mutation of sigK decreased the transcriptional activities of all these genes, indicating that the transcription of these genes is controlled by SigK. Furthermore, mutation of gerE decreased the transcriptional activities of bclB, bxpB, cotB, and iunH but increased the expression of bxpA, and GerE binds to the promoters of bclB, bxpB, cotB, bxpA, and iunH. These results suggest that GerE directly regulates the transcription of these genes, increasing the expression of bclB, bxpB, cotB, and iunH and decreasing that of bxpA. These findings provide insight into the exosporium assembly process at the transcriptional level.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Peng Q,Kao G,Qu N,Zhang J,Li J,Song F

doi

10.1038/srep19005

subject

Has Abstract

pub_date

2016-01-25 00:00:00

pages

19005

issn

2045-2322

pii

srep19005

journal_volume

6

pub_type

杂志文章
  • Establishment and characterization of patient-derived tumor xenograft using gastroscopic biopsies in gastric cancer.

    abstract::The patient-derived tumor xenograft (PDTX) model has become the most realistic model for preclinical studies. PDTX models of gastric cancer using surgical tissues are reported occasionally; however, the PDTX models using gastroscopic biopsies, which are best for evaluating new drugs, are unreported. In our study, a to...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep08542

    authors: Zhu Y,Tian T,Li Z,Tang Z,Wang L,Wu J,Li Y,Dong B,Li Y,Li N,Zou J,Gao J,Shen L

    更新日期:2015-02-25 00:00:00

  • Ecosystem experiment reveals benefits of natural and simulated beaver dams to a threatened population of steelhead (Oncorhynchus mykiss).

    abstract::Beaver have been referred to as ecosystem engineers because of the large impacts their dam building activities have on the landscape; however, the benefits they may provide to fluvial fish species has been debated. We conducted a watershed-scale experiment to test how increasing beaver dam and colony persistence in a ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep28581

    authors: Bouwes N,Weber N,Jordan CE,Saunders WC,Tattam IA,Volk C,Wheaton JM,Pollock MM

    更新日期:2016-07-04 00:00:00

  • A Targeted Mass Spectrometric Assay for Reliable Sensitive Hepcidin Quantification.

    abstract::Hepcidin, a cysteine-rich peptide hormone, secreted mainly by the liver, plays a central role in iron metabolism regulation. Emerging evidence suggests that disordered iron metabolism is a risk factor for various types of diseases including cancers. However, it remains challenging to apply current mass spectrometry (M...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-43756-9

    authors: Moghieb A,Tesfay L,Nie S,Gritsenko M,Fillmore TL,Jacobs JM,Smith RD,Torti FM,Torti SV,Shi T,Ansong C

    更新日期:2019-05-13 00:00:00

  • Improved adherence and treatment outcomes with an engaging, personalized digital therapeutic in amblyopia.

    abstract::Given the prevalence of poor adherence to therapy and the biases of self-reporting across healthcare, we hypothesized that an engaging, personalized therapy may improve adherence and treatment outcomes in the home. We tested this hypothesis in the initial indication of amblyopia, a neurodevelopmental disorder for whic...

    journal_title:Scientific reports

    pub_type: 临床试验,杂志文章

    doi:10.1038/s41598-020-65234-3

    authors: Xiao S,Gaier ED,Mazow ML,Stout AU,Travers DA,Angjeli E,Wu HC,Binenbaum G,Hunter DG

    更新日期:2020-05-20 00:00:00

  • Experimental Model for Successful Liver Cell Therapy by Lenti TTR-YapERT2 Transduced Hepatocytes with Tamoxifen Control of Yap Subcellular Location.

    abstract::Liver repopulation by transplanted hepatocytes has not been achieved previously in a normal liver microenvironment. Here we report that adult rat hepatocytes transduced ex vivo with a lentivirus expressing a human YapERT2 fusion protein (hYapERT2) under control of the hepatocyte-specific transthyretin (TTR) promoter r...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep19275

    authors: Yovchev M,Jaber FL,Lu Z,Patel S,Locker J,Rogler LE,Murray JW,Sudol M,Dabeva MD,Zhu L,Shafritz DA

    更新日期:2016-01-14 00:00:00

  • Low Temperature Vacuum Synthesis of Triangular CoO Nanocrystal/Graphene Nanosheets Composites with Enhanced Lithium Storage Capacity.

    abstract::CoO nanocrystal/graphene nanosheets (GNS) composites, consisting of a triangular CoO nanocrystal of 2~20 nm on the surface of GNS, are synthesized by a mild synthetic method. First, cobalt acetate tetrahydrate is recrystallized in the alcohol solution at a low temperature. Then, graphene oxide mixed with cobalt-precur...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep10017

    authors: Guan Q,Cheng J,Li X,Wang B,Huang L,Nie F,Ni W

    更新日期:2015-05-11 00:00:00

  • Effectiveness of the European Natura 2000 network to sustain a specialist wintering waterbird population in the face of climate change.

    abstract::Analysis of coordinated Greater Scaup (Aythya marila) count data from the last 30 years showed a 38.1% decrease in wintering numbers in North-West Europe, from 309,000 during 1988-1991 to c.192,300 individuals during 2015-2018. Annual trends in wintering numbers differed throughout the range. Numbers decreased in the ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-77153-4

    authors: Marchowski D,Ławicki Ł,Fox AD,Nielsen RD,Petersen IK,Hornman M,Nilsson L,Haas F,Wahl J,Kieckbusch J,Nehls HW,Calbrade N,Hearn R,Meissner W,Fitzgerald N,Luigujoe L,Zenatello M,Gaudard C,Koschinski S

    更新日期:2020-11-20 00:00:00

  • Steady streaming as a method for drug delivery to the inner ear.

    abstract::The inner ear, or cochlea, is a fluid-filled organ housing the mechanosensitive hair cells. Sound stimulation is relayed to the hair cells through waves that propagate on the elastic basilar membrane. Sensorineural hearing loss occurs from damage to the hair cells and cannot currently be cured. Although drugs have bee...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-79946-z

    authors: Sumner L,Mestel J,Reichenbach T

    更新日期:2021-01-08 00:00:00

  • Mental representation and episodic-like memory of own actions in dogs.

    abstract::We investigated whether dogs remember their spontaneous past actions relying on episodic-like memory. Dogs were trained to repeat a small set of actions upon request. Then we tested them on their ability to repeat other actions produced by themselves, including actions performed spontaneously in everyday situations. D...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-67302-0

    authors: Fugazza C,Pongrácz P,Pogány Á,Lenkei R,Miklósi Á

    更新日期:2020-06-26 00:00:00

  • New Routes for Refinery of Biogenic Platform Chemicals Catalyzed by Cerium Oxide-supported Ruthenium Nanoparticles in Water.

    abstract::Highly selective hydrogenative carbon-carbon bond scission of biomass-derived platform oxygenates was achieved with a cerium oxide-supported ruthenium nanoparticle catalyst in water. The present catalyst enabled the selective cleavage of carbon-carbon σ bonds adjacent to carboxyl, ester, and hydroxymethyl groups, open...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-017-14373-1

    authors: Mizugaki T,Togo K,Maeno Z,Mitsudome T,Jitsukawa K,Kaneda K

    更新日期:2017-10-25 00:00:00

  • Hyperspectral Imaging for Determining Pigment Contents in Cucumber Leaves in Response to Angular Leaf Spot Disease.

    abstract::Hyperspectral imaging technique was employed to determine spatial distributions of chlorophyll (Chl), and carotenoid (Car) contents in cucumber leaves in response to angular leaf spot (ALS). Altogether, 196 hyperspectral images of cucumber leaves with five infection severities of ALS were captured by a hyperspectral i...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep27790

    authors: Zhao YR,Li X,Yu KQ,Cheng F,He Y

    更新日期:2016-06-10 00:00:00

  • A high-resolution time-depth view of dimethylsulphide cycling in the surface sea.

    abstract::Emission of the trace gas dimethylsulphide (DMS) from the ocean influences the chemical and optical properties of the atmosphere, and the olfactory landscape for foraging marine birds, turtles and mammals. DMS concentration has been seen to vary across seasons and latitudes with plankton taxonomy and activity, and fol...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep32325

    authors: Royer SJ,Galí M,Mahajan AS,Ross ON,Pérez GL,Saltzman ES,Simó R

    更新日期:2016-08-31 00:00:00

  • Genetic dissection of host immune response in pneumonia development and progression.

    abstract::The role of host genetic variation in pneumonia development and outcome is poorly understood. We studied common polymorphisms in the genes of proinflammatory cytokines (IL6 rs1800795, IL8 rs4073, IL1B rs16944), anti-inflammatory cytokines (IL10 rs1800896, IL4 rs2243250, IL13 rs20541) and toll-like receptors (TLR2 rs57...

    journal_title:Scientific reports

    pub_type: 杂志文章,meta分析

    doi:10.1038/srep35021

    authors: Smelaya TV,Belopolskaya OB,Smirnova SV,Kuzovlev AN,Moroz VV,Golubev AM,Pabalan NA,Salnikova LE

    更新日期:2016-10-11 00:00:00

  • Novel metastable metallic and semiconducting germaniums.

    abstract::Group-IVa elements silicon and germanium are known for their semiconducting properties at room temperature, which are technologically critical. Metallicity and superconductivity are found at higher pressures only, Ge β-tin (tI4) being the first high-pressure metallic phase in the phase diagram. However, recent experim...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep01466

    authors: Selli D,Baburin IA,Martoňák R,Leoni S

    更新日期:2013-01-01 00:00:00

  • New Insight into AuNP Applications in Tumour Treatment and Cosmetics through Wavy Annuli at the Nanoscale.

    abstract::The purpose of this study is to probe the peristaltic propulsion of a non-Newtonian fluid model with suspended gold nanoparticles. The base fluid is considered to simulate blood using the Carreau fluid model. We model a small annulus as a tube with a peristaltic wave containing a clot propagating towards the tube wall...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-36459-0

    authors: Abdelsalam SI,Bhatti MM

    更新日期:2019-01-22 00:00:00

  • Seasonal flow dynamics exacerbate overlap between artisanal fisheries and imperiled Ganges River dolphins.

    abstract::Here we quantify the effects of artisanal fisheries on the ecology of a small cetacean, the Ganges River dolphin (Platanista gangetica gangetica, GRD), in a large river system of Nepal. We examine the size-classes of fisheries' catches, behavioural changes in GRD in response to fishing activities, and diel overlap bet...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-75997-4

    authors: Paudel S,Koprowski JL,Cove MV

    更新日期:2020-11-02 00:00:00

  • Morphology and growth speed of hcp domains during shock-induced phase transition in iron.

    abstract::Emergence and time evolution of micro-structured new-phase domains play a crucial role in determining the macroscopic physical and mechanical behaviors of iron under shock compression. Here, we investigate, through molecular dynamics simulations and theoretical modelings, shock-induced phase transition process of iron...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep03628

    authors: Pang WW,Zhang P,Zhang GC,Xu AG,Zhao XG

    更新日期:2014-01-10 00:00:00

  • Author Correction: Intermittent restraint stress induces circadian misalignment in the mouse bladder, leading to nocturia.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-019-53132-2

    authors: Ihara T,Nakamura Y,Mitsui T,Tsuchiya S,Kanda M,Kira S,Nakagomi H,Sawada N,Kamiyama M,Shigetomi E,Shinozaki Y,Yoshiyama M,Nakao A,Koizumi S,Takeda M

    更新日期:2019-11-08 00:00:00

  • Orientation toward humans predicts cognitive performance in orang-utans.

    abstract::Non-human animals sometimes show marked intraspecific variation in their cognitive abilities that may reflect variation in external inputs and experience during the developmental period. We examined variation in exploration and cognitive performance on a problem-solving task in a large sample of captive orang-utans (P...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep40052

    authors: Damerius LA,Forss SI,Kosonen ZK,Willems EP,Burkart JM,Call J,Galdikas BM,Liebal K,Haun DB,van Schaik CP

    更新日期:2017-01-09 00:00:00

  • Host-guest complexes of imazalil with cucurbit[8]uril and β-cyclodextrin and their effect on plant pathogenic fungi.

    abstract::We report the control of imazalil (IMZ) antifungal activity utilizing its non-covalent assembly with β-cyclodextrins (β-CD) and cucurbit[8]uril (CB8) macrocycles, as well as its stimuli-responsive disassembly with cadaverine. The NMR results are consistent with inclusion of a single IMZ molecule inside the cavities of...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-21156-9

    authors: Al-Dubaili N,El-Tarabily K,Saleh N

    更新日期:2018-02-12 00:00:00

  • Region-specific mechanisms of corticosteroid-mediated inotropy in rat cardiomyocytes.

    abstract::Regional differences in ion channel activity in the heart control the sequence of repolarization and may contribute to differences in contraction. Corticosteroids such as aldosterone or corticosterone increase the L-type Ca2+ current (ICaL) in the heart via the mineralocorticoid receptor (MR). Here, we investigate the...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-68308-4

    authors: Wacker C,Dams N,Schauer A,Ritzer A,Volk T,Wagner M

    更新日期:2020-07-14 00:00:00

  • Author Correction: Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo.

    abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...

    journal_title:Scientific reports

    pub_type: 已发布勘误

    doi:10.1038/s41598-020-71638-y

    authors: Liu H,Lv P,Zhu Y,Wu H,Zhang K,Xu F,Zheng L,Zhao J

    更新日期:2020-09-09 00:00:00

  • Fast Collisional Lipid Transfer Among Polymer-Bounded Nanodiscs.

    abstract::Some styrene/maleic acid (SMA) copolymers solubilise membrane lipids and proteins to form polymer-bounded nanodiscs termed SMA/lipid particles (SMALPs). Although SMALPs preserve a lipid-bilayer core, they appear to be more dynamic than other membrane mimics. We used time-resolved Förster resonance energy transfer and ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep45875

    authors: Cuevas Arenas R,Danielczak B,Martel A,Porcar L,Breyton C,Ebel C,Keller S

    更新日期:2017-04-05 00:00:00

  • Galectin-3 Enhances Migration of Minature Pig Bone Marrow Mesenchymal Stem Cells Through Inhibition of RhoA-GTP Activity.

    abstract::Bone marrow mesenchymal stem cells (BM-MSCs) are used in tissue engineering because of their migration characters. However, BM-MSCs have limitations in terms of reaching injuries and self-renewal. Therefore, enhancement of BM-MSC migration is important for therapeutic applications. Here, we assessed whether galectin-3...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep26577

    authors: Gao Q,Xia Y,Liu L,Huang L,Liu Y,Zhang X,Xu K,Wei J,Hu Y,Mu Y,Li K

    更新日期:2016-05-24 00:00:00

  • Objectum sexuality: A sexual orientation linked with autism and synaesthesia.

    abstract::Objectum-sexuality (OS) is a sexual orientation which has received little attention in the academic literature. Individuals who identify as OS experience emotional, romantic and/or sexual feelings towards inanimate objects (e.g. a bridge, a statue). We tested 34 OS individuals and 88 controls, and provide the first em...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-56449-0

    authors: Simner J,Hughes JEA,Sagiv N

    更新日期:2019-12-27 00:00:00

  • Effects of local structure of Ce(3+) ions on luminescent properties of Y3Al5O12:Ce nanoparticles.

    abstract::Ce(3+)-doped yttrium aluminum garnet (YAG:Ce) nanocrystals were successfully synthesized via a facile sol-gel method. Multiple characterization techniques were employed to study the structure, morphology, composition and photoluminescence properties of YAG:Ce nanophosphors. The YAG:Ce0.0055 sintered at 1030 °C exhibit...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep22238

    authors: He X,Liu X,Li R,Yang B,Yu K,Zeng M,Yu R

    更新日期:2016-03-03 00:00:00

  • Vital Recorder-a free research tool for automatic recording of high-resolution time-synchronised physiological data from multiple anaesthesia devices.

    abstract::The current anaesthesia information management system (AIMS) has limited capability for the acquisition of high-quality vital signs data. We have developed a Vital Recorder program to overcome the disadvantages of AIMS and to support research. Physiological data of surgical patients were collected from 10 operating ro...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-018-20062-4

    authors: Lee HC,Jung CW

    更新日期:2018-01-24 00:00:00

  • Intratumoral morphological heterogeneity of breast cancer: neoadjuvant chemotherapy efficiency and multidrug resistance gene expression.

    abstract::In this study, the influence of intratumoral morphological heterogeneity of breast cancer on neoadjuvant chemotherapy (NAC) efficiency was investigated. In particular, we analysed the association of NAC response and pre- and post-NAC expression of the main multidrug resistance (MDR) genes--ABCB1, ABCC1, ABCC5, ABCG1, ...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/srep04709

    authors: Denisov EV,Litviakov NV,Zavyalova MV,Perelmuter VM,Vtorushin SV,Tsyganov MM,Gerashchenko TS,Garbukov EY,Slonimskaya EM,Cherdyntseva NV

    更新日期:2014-04-16 00:00:00

  • Systematic evaluation of particle loss during handling in the percutaneous transluminal angioplasty for eight different drug-coated balloons.

    abstract::Paclitaxel drug coated balloons (DCBs) should provide optimal drug transfer exclusively to the target tissue. The aim of this study was to evaluate the particle loss by handling during angioplasty. A robotic arm was developed for systematic and reproducible drug abrasion experiments. The contact force on eight differe...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-020-74227-1

    authors: Heinrich A,Engler MS,Güttler FV,Matthäus C,Popp J,Teichgräber UK

    更新日期:2020-10-14 00:00:00

  • Mutational convergence acts as a major player in adaptive parallel evolution of Shigella spp.

    abstract::Shigella spp., emerging from multiple origins of Escherichia coli, poses a significant health threat as a causative agent of bacillary dysentery. While multiple serotypes of four different species have evolved via independent lineages, Shigella spp. are designated as a single pathotype, primarily because of their comm...

    journal_title:Scientific reports

    pub_type: 杂志文章

    doi:10.1038/s41598-019-39810-1

    authors: Thomas AK,Preetha S,Omanakuttan A,Vidyullata L,Ashokan A,Rajachandran V,Chattopadhyay S

    更新日期:2019-03-01 00:00:00