Abstract:
BACKGROUND:Since gamma interferon release assays (IGRAs) cannot differentiate between active tuberculosis and latent tuberculosis infection (LTBI), development of rapid and specific diagnosis tools are essential for discriminating between active tuberculosis (TB) from LTBI. Both IGRAs are based on Mycobacterium tuberculosis-specific antigens, namely, early secretory antigenic target 6 (ESAT-6) and 10kDa culture filtrate (CFP-10). The aim of this study was to evaluate the potential value of IL-2 secretion by whole blood cells after stimulation with rESAT-6 and rCFP-10 for discriminating between active and latent tuberculosis. METHODS:Interleukin-2 and IFN-γ were measured after blood stimulation of 90 cases (30 with active TB, 30 with LTBI and 30 healthy controls) with recombinant ESAT-6 and CFP-10. Receiver operating characteristic (ROC) curve analysis was conducted to determine the best IL-2 and IFN-γ result thresholds in discriminating between cases with active or latent TB, and the corresponding sensitivity and specificity were recorded. RESULTS:The IFN-γ release assay demonstrated a good sensitivity and specificity (sensitivity 83-84% and specificity 92%) for diagnosis of tuberculosis. The discrimination performance of IL-2 assay (assessed by the area under ROC curve) between LTBI and patients with active TB were 0.75 and 0.8 following stimulation with rESAT-6 and rCFP-10, respectively. Maximum discrimination was reached at a cut-off of 11.6pg/mL for IL-2 after stimulation with recombinant rESAT-6 with 72% sensitivity and 79% specificity and 10.7pg/mL for IL-2 following stimulation with rCFP-10 with 75% sensitivity and 79% specificity, respectively. CONCLUSION:This study demonstrates that rESAT-6 and rCFP-10 can provide a sensitive and specific diagnosis of TB. In addition, it was shown that IL-2 may be serving as a marker for discriminating LTBI and active TB.
journal_name
Allergol Immunopathol (Madr)journal_title
Allergologia et immunopathologiaauthors
Mamishi S,Pourakbari B,Shams H,Marjani M,Mahmoudi Sdoi
10.1016/j.aller.2015.09.004subject
Has Abstractpub_date
2016-07-01 00:00:00pages
314-21issue
4eissn
0301-0546issn
1578-1267pii
S0301-0546(15)00149-4journal_volume
44pub_type
杂志文章abstract:BACKGROUND:Asthma is the most common chronic disease affecting children and adolescents (AD). We evaluated the prevalence of asthma and associated factors in adolescents living in a developing region in northeast Brazil using the ISAAC (International Study of Asthma and Allergies in Childhood) methodology. METHODS:Acc...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2014.05.006
更新日期:2015-09-01 00:00:00
abstract::Excessive accumulation of histamine in the body leads to miscellaneous symptoms mediated by its bond to corresponding receptors (H1-H4). Increased concentration of histamine in blood can occur in healthy individuals after ingestion of foods with high contents of histamine, leading to histamine intoxication. In individ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:10.1016/j.aller.2015.05.001
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND:Although it has been hypothesized that infections may play a preventive role in allergic diseases, the role of Helicobacter pylori (H. pylori) is not clear. In this study we aimed to determine the association between H. pylori infection and allergic inflammation. METHODS:H. pylori infection was assessed in ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1157/13120393
更新日期:2008-03-01 00:00:00
abstract:BACKGROUND:The International Study of Asthma and Allergies in Childhood (ISAAC) identified trends in the prevalence of symptoms of asthma, rhinoconjunctivitis and eczema over a seven-year period. We hypothesised that environmental influences on the three diseases are different and therefore investigated the correlation...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,多中心研究
doi:10.1016/j.aller.2011.11.004
更新日期:2012-09-01 00:00:00
abstract::A short summary of the literature about the cytotoxic leucocyte test was presented. The results of 1824 CLTs involving 23 food allergens were analyzed. Of the foods tested malt, orange, rye, milk, corn, tomato, oats, wheat, brewers yeast and rice were the ten foods giving a reaction most often in the CLT. Except for m...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::All classes of immunoglobulins and albumin have been studied in different phases of disease, both in blood and in the nasal secretions of 75 patients with pollen allergy. There were 20 healthy persons in the test group. Total correlational analysis was done to determine interdependence of the researched indexes. The p...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1990-11-01 00:00:00
abstract:BACKGROUND:The pathogenesis of atopic dermatitis (AD) is still not completely understood. AD is characterized by the presence of clinical symptoms of both IgE antibody-mediated immediate hypersensitivity and specific T lymphocyte-mediated delayed hypersensitivity. OBJECTIVE:To evaluate the immunological mechanisms inv...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(04)79251-4
更新日期:2004-09-01 00:00:00
abstract::The increasingly extensive use of selective serotonin reuptake inhibitors will probably increase the number of cases of cutaneous reactions due to these drugs. This report describes a case of generalized exanthema due to paroxetine. Positive results to patch tests indicated delayed hypersensitivity. ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(06)73527-3
更新日期:2006-05-01 00:00:00
abstract::A procedure is described for the cytochemical detection of esterase alpha naphtyl acetate (ANAE) and beta glucuronidase (BG) activity in the circulating lymphocytes of man. The number of BG positive cells (TBG) as evaluated on the smears of Ficoll Urovision purified cells, correlated well with the number of T gamma (E...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
abstract:BACKGROUND:Leukotrienes are among the most important mediators associated with inflammatory responses in patients with exercise induced asthma (EIA). The aim of this study was to investigate the impact of exercise on the urinary leukotriene profile. Hence, we compared post exercise changes of urinary leukotriene E4 (LT...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2011.03.012
更新日期:2012-05-01 00:00:00
abstract::The percentages and absolute numbers of T, B and "null" cells were studied in 100 allergic patients and compared with values observed in 35 healthy age matched control subjects. T cells were identified by their ability to form spontaneous rosettes with SRBC (E-RFC) and B cells by their complement receptor that allows ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::The present prospective, open, observational study assessed the effect of the use of standard environmental control measures together with the application of an acaricide-containing aerosol of esbiol/benzyl benzoate/piperonyl butoxide/2-phenylphenol (Frontac) on several clinical and environmental parameters in patient...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,多中心研究
doi:10.1016/s0301-0546(02)79096-4
更新日期:2002-03-01 00:00:00
abstract:BACKGROUND:Analgesic intolerance (AI) sometimes appear alone and sometimes with bronchial asthma affecting about 10% of asthmatics and sometimes before and the other times after asthma. OBJECTIVE:We investigated the possible clinical risk factors which might be affecting the transition from isolated AI to analgesic in...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::The aminoacid contents of native chestnut and its powdered form were studied. It was found that it has low cystine and methionine contents but if it is supplemented accordingly, the chestnut pulp can well be used for infant feeding. More than 50 infants were fed with chestnuts, and only in two cases was there intolera...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1976-11-01 00:00:00
abstract::The term food allergy refers to the immune reaction (mediated by IgE or otherwise) that develops in response to the ingestion of a concrete type of food. Among the different potential manifestations of an allergic reaction, those exclusively affecting the gastrointestinal system are described. In recent years, the stu...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:10.1016/s0301-0546(09)70250-2
更新日期:2009-01-01 00:00:00
abstract::After isolating the soluble factors and observing their biological activity of "in vitro" IgE synthesis, we decided to study the changes these molecules exercise on the expression of lymphocytes of IgE (FcR epsilon). We saw that the fractions from healthy subjects inhibited the expression of receptors quantified by im...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::A better understanding of the ocular immune responses should help us determine the etiology and pathophysiology of many uveitides. Ocular immune "privilege" is the result of the interaction of factors such as the blood-ocular barrier, the absence of lymphatic vessels, the sequestration of retinal antigens, the presenc...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:
更新日期:1994-01-01 00:00:00
abstract::In clinical allergology and particularly during childhood, we quite frequently encounter patients who are allergic to milk. This has usually been confirmed by the clinical history and by skin testing. Statistics show a sensitization to cows' milk of between 0.3 and 7% of the world's population. In view of the diversit...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1978-05-01 00:00:00
abstract::A retrospective study on 52,900 individuals tested in 177 Italian Medical Centres for AIDS and related syndromes is reported. The data confirm that I.V. drug users are the group at highest risk in Italy (46% were seropositive and more than one half of these presented PGL or ARC) and that the epidemic probably started ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::This study investigated the specificity and efficacy of immunotherapy with Artemisia vulgaris (Artemisia) extract. We studied 25 patients with a more than two year lasting history of seasonal rhinoconjunctivitis and who had only two clinically important allergies, either to Artemisia and Betula verrucosa (Betula) or t...
journal_title:Allergologia et immunopathologia
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1995-03-01 00:00:00
abstract:BACKGROUND:Children with IgE-mediated cow's milk allergy (IgE-CMA) often tolerate baked milk within a wheat matrix. In our study we evaluated the impact of wheat matrix and of little standardised cooking procedures on tolerance of baked milk. We also tested tolerance versus parmigiano reggiano (PR) and whey-based parti...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2016.03.005
更新日期:2016-01-01 00:00:00
abstract::The effect of intra-lymphatic administration of Lipiodol-l131 on the number of T and B lymphocytes, and on the in vivo response to common antigens and to DNCB, was investigated in uremic patients in the pre-transplant period. T lymphocytes have been detected by rosette formation with sheep erythrocytes (E) and B lymph...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1975-09-01 00:00:00
abstract::Shellfish is one of the most frequent causes of food allergy. We studied 48 patients (25 male and 23 female) with a mean age of 24.2 +/- 1.8 with shellfish hypersensitivity. A clinical questionnaire was carried out and prick tests were performed using a series of aeroallergens and a battery of extracts of squid, shrim...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:
更新日期:1994-03-01 00:00:00
abstract::Since the first decades of the twentieth century, some authors have believed bacterial respiratory infection to be an important triggering factor in bronchial asthma, drawing attention to an asthmatic response to infection. In this context, already in 1995, we presented a study on nasal secretion cultures and the rela...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章,评审
doi:10.1016/s0301-0546(01)79035-0
更新日期:2001-05-01 00:00:00
abstract::The results of the cutaneous tests with fungus, and the repercussion of the fungal allergens in asthma via inhalatory route has been a controversial subject for many years. The lack of characterization, purification and standardization of the allergens make the results of the cutaneous tests highly variable. The diagn...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:
更新日期:1986-07-01 00:00:00
abstract:BACKGROUND:The clinical relevance of elevated basal serum tryptase (eBST ≥ 11.4 ng/ml) often remains unclear. METHODS:BST was assessed in 15,298 patients attending our outpatient clinic. Frequency and severity of anaphylaxis was compared in 900 patients with eBST and 900 patients with normal BST. The prevalence of eBS...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2014.05.002
更新日期:2014-11-01 00:00:00
abstract:BACKGROUND:The sharon fruit is the edible fruit of the persimmon tree (Diospyros kaki) which belongs to the Ebenaceae family. There are few references of allergic reaction to this fruit. We introduce a case of a 33-years old man with a anaphylactic reaction immediately after ingestion of sharon fruit. METHODS:Skin pri...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/s0301-0546(01)79021-0
更新日期:2001-03-01 00:00:00
abstract:BACKGROUND:Assessment of asthma with a control test has been suggested as a relevant approach in recent years. However, no biomarker of systemic inflammation has been included in the assessment of asthma control. OBJECTIVE:To evaluate plasma paraoxonase (PON1), total oxidant status (TOS), and total antioxidant status ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2014.05.009
更新日期:2015-07-01 00:00:00
abstract:BACKGROUND:Migrants from developing to Western countries tend to become more sensitised to host than to origin country allergens, although substantial changes in migration patterns have occurred in recent decades. METHODS:We investigated adult immigrants with respiratory allergy, first tested for allergic sensitisatio...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2014.07.007
更新日期:2015-09-01 00:00:00
abstract:BACKGROUND:Neutrophil-lymphocyte ratio (NLR) is a test used to evaluate the systemic inflammation. There is little knowledge about the neutrophil-lymphocyte ratio in asthmatics. In our study, we aimed to evaluate NLR and to assess its relationship with clinical parameters in children with asthma. METHODS:Four hundred ...
journal_title:Allergologia et immunopathologia
pub_type: 杂志文章
doi:10.1016/j.aller.2015.09.005
更新日期:2016-07-01 00:00:00