Expression of mTOR and its inhibitory effect on cell proliferation and apoptosis of breast cancer cells.

Abstract:

:The aim of this study is to investigate the expression of mTOR in breast cancer and observe the effect of CCI-779 on proliferation and apoptosis of MDA-MB-231 cells. Immunohistochemical staining was used to detect the expression of mTOR protein in breast cancer tissues and MDA-MB-231 cells. MTT assay was used to assess the effect of CCI-779 on proliferation of MDA-MB-231 cells. Annex-inV-FITC/ PI assay was utilized to evaluate the effect of CCI-779 on apoptosis of MDA-MB-231 cells. Among the 71 cases of breast cancer tissues, 54.9% were mTOR-positive that exhibited significantly higher expression than the 32 cases of normal tissues (21.9%); mTOR protein was also found to be expressed in MDA-MB-231 cells. The mTOR inhibitor CCI-779 significantly inhibited the proliferation of MDA-MB-231 cells that was dose- and time-dependent. However, CCI-779 was unable to induce apoptosis of MDA-MB-231 cells as demonstrated with AnnexinV-FITC/PI assay. mTOR plays a key role in the initiation and development of breast cancer, and its inhibitor CCI-779 exerts a strong suppressive activity against MDA-MB-231 cells, suggesting its therapeutic potential to treat breast cancer.

authors

Cheng XF,Liu Q,Zhang XF,Zhao HD,Wang W,Chu AJ

subject

Has Abstract

pub_date

2015-10-01 00:00:00

pages

869-73

issue

4

eissn

0393-974X

issn

1724-6083

pii

17

journal_volume

29

pub_type

信件
  • Newborn screening of inherited metabolic disorders: the Italian situation.

    abstract::Starting from an international overview of the current status of screening programs, the present paper focuses on the legal situation in Italy and the great differences among Italian regions. Since the introduction of tandem mass spectrometry (MS/MS) in the ‘90s the paradigm “one spot-one disease” changed. Only recent...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Focardi M,Pinchi V,Defraia B,Gualco B,Varvara G,Norelli GA

    更新日期:2016-07-01 00:00:00

  • Short term efficacy of capacitive-resistive diathermy therapy in patients with low back pain: a prospective randomized controlled trial.

    abstract::To compare the effectiveness of Doctor Tecar TherapyTM with that of laser therapy in the management of low back pain (LBP), a total of 60 patients with LBP were enrolled. The participants were randomly divided into two groups: a Tecar group (experimental group, 30 subjects), and a laser group (control group, 30 subjec...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章,随机对照试验

    doi:

    authors: Notarnicola A,Maccagnano G,Gallone MF,Covelli I,Tafuri S,Moretti B

    更新日期:2017-04-01 00:00:00

  • Thyroid hormone activates rat liver adenosine 5,-monophosphate-activated protein kinase: relation to CaMKKb, TAK1 and LKB1 expression and energy status.

    abstract::AMP-activated protein kinase (AMPK) is a sensor of energy status supporting cellular energy homeostasis that may represent the metabolic basis for 3,3,,5-triiodo-L-thyronine (T3) liver preconditioning. Functionally transient hyperthyroid state induced by T3 (single dose of 0.1 mg/kg) in fed rats led to upregulation of...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Vargas R,Ortega Y,Bozo V,Andrade M,Minuzzi G,Cornejo P,Fernandez V,Videla LA

    更新日期:2013-10-01 00:00:00

  • Mast cell and cancer with special emphasis on il-37 an anti-inflammatory and inhibitor of innate immunity: new frontiers.

    abstract::Mast cells (MCs) are mediators of allergy and inflammation and participate in the growth of cancer cells. MCs can promote both neoangiogenesis and tumor growth. They increase in the stroma of certain tumors where they can be recruited by tumor-derived chemoattractants, such as monocyte chemotactic protein-1 (MCP-1), R...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 社论

    doi:

    authors: Carinci F,Lessiani G,Spinas E,Kritas SK,Ronconi G,Caraffa Al,Conti P

    更新日期:2016-10-01 00:00:00

  • Early and late behavioral changes in sciatic nerve injury may be modulated by nerve growth factor and substance P in rats: a chronic constriction injury long-term evaluation.

    abstract::Chronic constriction injury (CCI) simulates the symptoms of chronic nerve compression, which is characterized by allodynia and hyperalgesia. Nerve growth factor (NGF) is released after nerve injury by immune and Schwann cells and transported in retrograde fashion to the dorsal root ganglion (DRG), resulting in increas...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Da Silva JT,Evangelista BG,Venega RAG,Oliveira ME,Chacur M

    更新日期:2017-04-01 00:00:00

  • Neuropeptide NGF mediates neuro-immune response and inflammation through mast cell activation.

    abstract::Human mast cells (first described in 1879 by Paul Ehrlich) develop from committed precursors in the bone marrow expressing the differentiation marker CD34+ and distinct from the three other myeloid cells. Mast cells are present in various tissues especially near blood vessels, epithelia and nerves and they are activat...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 社论,评审

    doi:

    authors: Kritas SK,Saggini A,Cerulli G,Caraffa A,Antinolfi P,Pantalone A,Frydas S,Rosati M,Tei M,Speziali A,Saggini R,Pandolfi F,Conti P

    更新日期:2014-04-01 00:00:00

  • Study of GSK3b inhibitors SB415286 and SB216763 to improve osteoblastic differentiation on microstructured titanium.

    abstract::Rough titanium surfaces enhance cell response to activation of Wnt canonical signalling, a pathway required for osteoblast differentiation. The present study investigated the effects of GSK3β-inhibitors SB216763 and SB415286 on osteoblastic differentiation on titanium surfaces with different topography and wettability...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Lumetti S,Calciolari E,Parisi L,Toffoli A,Mazzotta S,Ferrillo S,Ierardo G,Macaluso GM,Galli C,Manfredi E

    更新日期:2017-07-01 00:00:00

  • Cyclin-dependent kinase 7 is a potential therapeutic target in papillary thyroid carcinoma.

    abstract::Given the pathological incidence of metastases or radioiodine-refractory papillary thyroid carcinoma (PTC) is increasing worldwide, patients have little alternatives when choosing effective drugs. Therefore, it is necessary to develop new therapeutic targets for PTC treatment. CDK7 is a member of the cyclindependent p...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Gong Y,Yang J,Liu F,Li Z,Gong R,Wei T

    更新日期:2018-11-01 00:00:00

  • HPV-16 E6-E7 differential transcription induced in Siha cervical cancer cell line by interferons.

    abstract::The transcription of HPV-16 E6/E7 RNA has been studied by the polymerase chain reaction (PCR) in the Siha cervical carcinoma cell line. With this technique, all three different transcripts have been detected: the cDNA colinear E6 form, and the two differentially spliced forms E6*I and E6*II. All types of RNA appear le...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: De Marco F,Marcante ML

    更新日期:1993-01-01 00:00:00

  • Obese visceral adipose tissue grafted in lean mice can alter glucose homeostasis and energy efficiency.

    abstract::Fat transplantation experiments have previously shown regulatory properties of lean adipose tissue on glucose homeostasis in the whole animal. In the current study, we addressed whether obese visceral white adipose tissue grafted in lean mice could alter glucose homeostasis. Obese visceral fat (VF) tissue was effectiv...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Barrera C,Gatica A,Morgan C

    更新日期:2012-07-01 00:00:00

  • How to obtain better results, choice of implant or surgical technique? A new surgical technique with an extramedullary referenced cutting guide and a blended alignment method.

    abstract::Measured resection and ligament balancing techniques are the most used in TKA; both require, in the majority of knee arthroplasty systems, a femoral intramedullary canal referencing for the distal femoral cut. Fat embolism, activation of coagulation and bleeding may occur from the reamed canal; this can be avoided wit...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Rossi SMP,Ghiara M,Castelli A,Ivone A,Jannelli E,Benazzo F

    更新日期:2018-11-01 00:00:00

  • The use of autologous platelet and plasma products in salvage neck dissections: a prospective clinical study evaluating early and late wound healing.

    abstract:OBJECTIVES:To evaluate the effect of autologous platelet and plasma adhesives (APA) on postoperative drainage and soft-tissue fibrosis following neck dissections. DESIGN:This was a blinded comparative prospective cohort study done as two parts: part one evaluated early post-surgical outcomes and part two evaluated lat...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Yoo J,Chandarana S,Fung K,Franklin JH,Nichols AC,Doyle PC

    更新日期:2012-04-01 00:00:00

  • Effects of anesthesia using propofol and etomidate on T lymphocyte subpopulation of infectious shock patients in perioperative period.

    abstract::Infectious or septic shock is induced when a toxic microorganism invades blood circulation in the human body. Emergency operation is an effective method for treating infectious shock in the early stages although the use of anesthesia is more complex due to the internal disorders caused by the disease. This study explo...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Wang Y,Zhang J,Zhang SJ

    更新日期:2017-01-01 00:00:00

  • Concomitant assessment of DNA oxidation and bone resorption over a rapid body mass reduction period in female judokas.

    abstract::The purpose of this study was to concomitantly determine oxidative DNAdamage and bone resorption following a rapid body mass reduction in association with energy restriction and exercise training, considering 17β-estradiol level, in female collegiate judokas. Eighteen nationally ranked university female judokas were e...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Yasuda N,Yano T

    更新日期:2018-07-01 00:00:00

  • T lymphocyte dependence of the immune response to immunosuppressive neoantigen-exposing Fc fragments of IgG.

    abstract::We recently identified a rheumatoid factor associated with autoimmune disease resistance and remission, and have named it regulatory rheumatoid factor (regRF). Epitopes recognized by regRF can be induced in papain Fc fragments of IgG. Immunization of arthritic rats with homologous Fc fragments that expose neoepitopes ...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 信件

    doi:

    authors: Stolyarova E,Beduleva L,Menshikov I,Sidorov A,Khramova T

    更新日期:2019-05-01 00:00:00

  • Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.

    abstract::Calcium phosphate ceramics have been applied in bone replacement for several decades due to their excellent biocompatibility, bioactivity, osteo-conductivity and mechanical strength. Several studies have demonstrated that porous hydroxyapatite (HA) is an excellent scaffold for osteogenic proliferation and differentiat...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Santarelli A,Mascitti M,Orsini G,Memè L,Rocchetti R,Tiriduzzi P,Sampalmieri F,Putignano A,Procaccini M,Lo Muzio L,Bambini F

    更新日期:2014-07-01 00:00:00

  • THIN-LAYER CHROMATOGRAPHY IDENTIFICATION FOR RHUBARB AND PHELLODENDRI AMURENSIS CORTEX IN SHUANG-BAI CATAPLASM AND STUDY OF SKIN IRRITATION ASSAY.

    abstract::This paper aimed to raise a thin-layer chromatography (TLC) identification method for rhubarb and Phellodendri Amurensis Cortex and inspected skin irritation induced by them. It applied the TLC identification for Rhubarb and Phellodendri Amurensis Cortex in Shuang-bai cataplasm prescription. In this study six rabbits ...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 信件

    doi:

    authors: Tian YG,Qin SM,Ding L

    更新日期:2015-04-01 00:00:00

  • Dimensional and microbiological in vitro analysis of a dental implant locking taper connection.

    abstract::The present study was carried out to compare the differences in contact, height and contact area between the implant-abutment interface and the implant-healing cap interface of an implant system featuring a locking tapered connection by using X-ray micro-tomography. It was also conducted to test in vitro whether the i...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 信件

    doi:

    authors: Giorgini G,Santangelo R,Bedini R,Pecci R,Manicone PF,Raffaelli L,Sanguinetti M,D ' Addona A

    更新日期:2013-10-01 00:00:00

  • C-reactive protein correlates to functional score systems of patients in general surgical intensive care unit.

    abstract::The aim of our study was to document the relationship between inflammation indicated by the serum levels of widespread acute-phase proteins and scoring systems used in assessing patient’s functional condition. We therefore assessed serum levels of c-reactive protein (CRP) and four clinical tests [acutephysiology and c...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Tian J,Liu X,Liu D

    更新日期:2016-01-01 00:00:00

  • Hemodialysis in children: how, when and why.

    abstract::End-stage renal diseases requiring chronic dialysis are rare in childhood and adolescence, but they are associated with high mortality and impaired quality of life (1, 2). The most common disease that causes chronic kidney disease (CKD) is primary glomerular disease (GD), followed by congenital abnormalities of the ki...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 临床试验

    doi:

    authors: special issue: “Focus on pediatric nephrology”.,Chimenz R,Fede C,Di Benedetto V,Concolino D,Scuderi MG,Salvo V,Gitto E,Cucinotta U,Viola V,Betta P,Cannavò L,Cuppari C

    更新日期:2019-09-01 00:00:00

  • SARS-CoV-2, which induces COVID-19, causes kawasaki-like disease in children: role of pro-inflammatory and anti-inflammatory cytokines.

    abstract::Acute severe respiratory syndrome coronavirus-2 (SARS-CoV-2) caused a global pandemic coronavirus disease 2019 (COVID-19). In humans, SARS-CoV-2 infection leads to acute respiratory distress syndrome which presents edema, hemorrhage, intra-alveolar fibrin deposition, and vascular changes characterized by thrombus form...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 社论

    doi:10.23812/EDITORIAL-RONCONI-E-59

    authors: Ronconi G,Teté G,Kritas SK,Gallenga CE,Caraffa Al,Ross R,Conti P

    更新日期:2020-05-01 00:00:00

  • Protective effect of a poly-phytocompound on early stage nephropathy secondary to experimentally-induced diabetes.

    abstract::Diabetic nephropathy (DN) is a severe and life-threatening complication of long-standing diabetes. As one of the main causes of end-stage renal disease, the prevention and treatment of DN in early stage, and the slowing down of DN progression are of utmost importance and are topics of several ongoing research studies....

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Marotta F,Harada M,Dallah ED,Yadav H,Solimene U,Di Lembo S,Minelli E,Jain S,Chui DH

    更新日期:2010-01-01 00:00:00

  • Reactive phenotypes after acute and chronic NK-cell activation.

    abstract::Several phenotypic changes have been shown to occur after NK-cell stimulation, involving molecules that have been proved to regulate NK-cell migration into tissues and NK-cell activation and proliferation as well as target cell recognition and killing. Here, we review the reactive phenotypes observed in vivo after acu...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Lima M,Almeida J,Teixeira MA,Santos AH,Queirós ML,Fonseca S,Moura J,Gonçalves M,Orfão A,Pinto Ribeiro AC

    更新日期:2004-07-01 00:00:00

  • Periodontitis and cerebrovascular disease: a new novel in medicine.

    abstract::The aim of this review is to determine if there is a relationship between periodontal disease and stroke. The included case-control and cohort studies mediate the incidence of stroke and periodontal disease by analyzing different parameters. A literature review was carried out in PubMed, Scopus and Embase databases us...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章,评审

    doi:

    authors: DENTAL SUPPLEMENT.,Campanella V,Oberti L,Gabrione F,González-Valero L,Hernández-Martínez V,Silvestre-Rangil J

    更新日期:2019-05-01 00:00:00

  • The use of hyaluronic acid in the treatment of shoulder capsulitis: a systematic review.

    abstract::Adhesive capsulitis (AC) is a common pathological condition of the shoulder characterized by painful restriction of range of motion (ROM) of the glenohumeral joint. Currently, no consensus has been reached regarding the best treatment. Hyaluronic acid (HA) injection is a safe procedure that can result in significant i...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Papalia R,Tecame A,Vadalà G,Russo F,Perna M,Papalia G,Bressi F,Sterzi S,Denaro V

    更新日期:2017-12-27 00:00:00

  • Inhibition of the differentiation of 3T3-L1 cells by interferon-beta and difluoromethyl ornithine.

    abstract::Both mouse interferon-beta (MuIFN-beta) and the inhibitor of ornithine decarboxylase (ODC), alpha-difluoromethyl ornithine (DFMO), inhibited the differentiation of mouse 3T3-L1 fibroblasts into adipocytes in a dose-dependent manner. DFMO and MuIFN-beta added together to cultures that were induced to differentiate prod...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:

    authors: Taylor JL,Turo KA,McCann PP,Grossberg SE

    更新日期:1988-01-01 00:00:00

  • Observation of the clinical effects of iontophoresis of a chinese drug in the treatment of degenerative osteoarthropathy.

    abstract::Degenerative osteoarthropathy, a kind of arthrosis induced by various factors, mainly affects articular cartilage and causes syndesmophyte formation. Its morbidity increases year by year, tending to occur more among young people than previously. This paper mainly discusses the clinical effects of iontophoresis of the ...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 信件,随机对照试验

    doi:

    authors: Geng XL,Sun XH,Zhang J,Yang LB,Liang QD

    更新日期:2015-01-01 00:00:00

  • Caveolin-1 improves lung injury in rats with chronic obstructive pulmonary disease partially through Wnt/β-catenin signaling pathway.

    abstract::The aim of this study was to explore the effects of Caveolin-1 on lung injury in rats with chronic obstructive pulmonary disease (COPD). The forced expiratory volume in 0.3 s/forced vital capacity (FEV0.3/ FVC) and peak expiratory flow (PEF) were determined. The total white blood cells (WBC), neutrophil ratio (NEUT%),...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 信件

    doi:10.23812/20-71-A-37

    authors: Guan P,Cai WT,Jiang F,Li W,Zeng M,Wu J

    更新日期:2020-03-01 00:00:00

  • Ticagrelor for patients with ST-segment elevation myocardial infarction after emergency percutaneous coronary intervention.

    abstract::Acute ST-segment elevation myocardial infarction (STEMI) is one of the most common cardiovascular emergencies. With the improvement of living standards, the incidence and mortality rate of STEMI has shown a significant growth trend. ...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 信件

    doi:

    authors: Han L,Zhang JJ,Jing HF,Qin L

    更新日期:2019-03-01 00:00:00

  • β-Carotene improves intestinal barrier function by modulating proinflammatory cytokines and improving antioxidant capacity in β-lactoglobulin-sensitized mice.

    abstract::Increased intestinal permeability due to barrier dysfunction is supposed to cause several gastrointestinal diseases. We have previously demonstrated that a single β-carotene (BC) dose protects against increase in anaphylactic response in β-lactoglobulin (BLG)-sensitized mice with no effect on the epithelial permeabili...

    journal_title:Journal of biological regulators and homeostatic agents

    pub_type: 杂志文章

    doi:10.23812/20-24-A

    authors: Grar H,Dib W,Gourine H,Negaoui H,Taleb BHF,Louaar A,Ouldhocine S,Kaddouri H,Kheroua O,Saidi D

    更新日期:2020-09-01 00:00:00