Abstract:
:Radish cytoplasmic male sterility (CMS) has been widely used for breeding in Raphanus and Brassica genera. However, the detailed regulation network of the male sterility remains to be determined. Our previous work has shown that the abnormalities in a CMS radish appeared shortly after the tetrad stage when microspores were malformed and the tapetal cells grew abnormally large. In this work, histological analysis shows that anthers are at the tetrad stage when the radish buds are about 1.5 mm in length. Furthermore, a high throughput RNA sequencing technology was employed to characterize the transcriptome of radish buds with length about 1.5 mm from two CMS lines possessing the CMS-inducing orf138 gene and corresponding near-isogenic maintainer lines. A total of 67,140 unigenes were functionally annotated. Functional terms for these genes are significantly enriched in 55 Gene Ontology (GO) groups and 323 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The transcriptome detected transcripts for 72 out of a total of 79 protein genes encoded in the chloroplast genome from radish. In contrast, the radish mitochondrial genome contains 34 protein genes, but only 16 protein transcripts were detected from the transcriptome. The transcriptome comparison between CMS and near-isogenic maintainer lines revealed 539 differentially expressed genes (DEGs), indicating that the false positive rate for comparative transcriptome profiling was clearly decreased using two groups of CMS/maintainer lines with different nuclear background. The level of 127 transcripts was increased and 412 transcripts were decreased in the CMS lines. No change in levels of transcripts except CMS-inducing orf138 was identified from the mitochondrial and chloroplast genomes. Some DEGs which would be associated with the CMS, encoding MYB and bHLH transcription factors, pentatricopeptide repeat (PPR) proteins, heat shock transcription factors (HSFs) and heat shock proteins (HSPs), are discussed. The transcriptome dataset and comparative analysis will provide an important resource for further understanding anther development, the CMS mechanism and to improve molecular breeding in radish.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Mei S,Liu T,Wang Zdoi
10.3390/ijms17010042subject
Has Abstractpub_date
2016-01-06 00:00:00issue
1issn
1422-0067pii
ijms17010042journal_volume
17pub_type
杂志文章abstract::Salidroside (Sal) is a potent antitumor drug with high water-solubility. The clinic application of Sal in cancer therapy has been significantly restricted by poor oral absorption and low tumor cell uptake. To solve this problem, lipid-shell and polymer-core nanoparticles (Sal-LPNPs) loaded with Sal were developed by a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15033373
更新日期:2014-02-25 00:00:00
abstract::Compared to solid tumors, the role of PD-L1 in hematological malignancies is less explored, and the knowledge in this area is mostly limited to lymphomas. However, several studies indicated that PD-L1 is also overexpressed in myeloid malignancies. Successful treatment of the acute myeloid leukemia (AML) is likely asso...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20112823
更新日期:2019-06-10 00:00:00
abstract::Endothelin-1 (ET-1) autocrine and paracrine signaling modulate cell proliferation of tumor cells by activating its receptors, endothelin A receptor (ETAR) and endothelin B receptor (ETBR). Dysregulation of ETAR activation promotes tumor development and progression. The potential of ETAR antagonists and the dual-ETAR a...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms17081244
更新日期:2016-08-02 00:00:00
abstract::Chronic obstructive pulmonary disease (COPD) is a major global cause of morbidity and mortality, projected to become the 3rd cause of disease mortality worldwide by 2020. COPD is characterized by persistent and not fully reversible airflow limitation that is usually progressive and is associated with an abnormal chron...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18112449
更新日期:2017-11-18 00:00:00
abstract::Rhizobia are Gram-negative bacteria that can exist either as free-living bacteria or as nitrogen-fixing symbionts inside root nodules of leguminous plants. The composition of the rhizobial outer surface, containing a variety of polysaccharides, plays a significant role in the adaptation of these bacteria in both habit...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms12117898
更新日期:2011-01-01 00:00:00
abstract::Apocynin, also known as acetovanillone, is a natural organic compound structurally related to vanillin. Apocynin is known to be an inhibitor of NADPH (Nicotinamide adenine dinucleotide phosphate) oxidase activity and is highly effective in suppressing the production of superoxide. The neuroprotective effects of apocyn...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19103087
更新日期:2018-10-09 00:00:00
abstract::The diversity and secondary metabolite potential of culturable actinomycetes associated with eight different marine sponges collected from the South China Sea and the Yellow sea were investigated. A total of 327 strains were isolated and 108 representative isolates were selected for phylogenetic analysis. Ten families...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13055917
更新日期:2012-01-01 00:00:00
abstract::The role of dynamic processes on self-assembled structures of 4'-([2,2':6', 2''-terpyridin]-4'-yl)-[1,1'-biphenyl]-4-carboxylic acid (l) molecules on Au(III) has been studied by scanning tunneling microscopy. The as-deposited monolayer is closed-packed and periodic in a short-range due to dipole forces. A thermal anne...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms14035686
更新日期:2013-03-11 00:00:00
abstract::Mineralization of hydrogel biomaterials with calcium phosphate (CaP) is considered advantageous for bone regeneration. Mineralization can be both induced by the enzyme alkaline phosphatase (ALP) and promoted by calcium-binding biomolecules, such as plant-derived polyphenols. In this study, ALP-loaded gellan gum (GG) h...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms21072315
更新日期:2020-03-27 00:00:00
abstract::The ability to respond flexibly to changing environmental circumstances is a hallmark of goal-directed behavior, and compromised flexibility is associated with a wide range of psychiatric conditions in humans, such as addiction and stress-related disorders. To identify neural circuits and transmitter systems implicate...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20123098
更新日期:2019-06-25 00:00:00
abstract::Acidosis commonly exists in the tissue microenvironment of various pathophysiological conditions such as tumors, inflammation, ischemia, metabolic disease, and respiratory disease. For instance, the tumor microenvironment is characterized by acidosis and hypoxia due to tumor heterogeneity, aerobic glycolysis (the "War...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18020278
更新日期:2017-01-27 00:00:00
abstract::Metformin is a biguanide drug that has been used to treat type 2 diabetes mellitus for more than 60 years. The United Kingdom Prospective Diabetic Study (UKPDS) has shown metformin to improve mortality rates in diabetes patients, and recent studies suggest metformin has additional effects in treating cancer, obesity, ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19102863
更新日期:2018-09-21 00:00:00
abstract::Our laboratory and others have previously shown that cannabinoid receptor type-1 (CB1r) activity is neuroprotective and a modulator of brain ageing; a genetic disruption of CB1r signaling accelerates brain ageing, whereas the pharmacological stimulation of CB1r activity had the opposite effect. In this study, we have ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22010005
更新日期:2020-12-22 00:00:00
abstract::The translation of metallothioneins (MTs) is one of the defense strategies by which organisms protect themselves from metal-induced toxicity. MTs belong to a family of proteins comprising MT-1, MT-2, MT-3, and MT-4 classes, with multiple isoforms within each class. The main aim of this study was to determine the behav...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms18030610
更新日期:2017-03-11 00:00:00
abstract::Transforming growth factor β (TGFβ) is a secreted growth and differentiation factor that influences vital cellular processes like proliferation, adhesion, motility, and apoptosis. Regulation of the TGFβ signaling pathway is of key importance to maintain tissue homeostasis. Perturbation of this signaling pathway has be...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms22020476
更新日期:2021-01-06 00:00:00
abstract::Methylation of several lysine residues of histones is a crucial mechanism for relatively long-term regulation of genomic activity. Recent molecular biological studies have demonstrated that the function of histone methylation is more diverse and complex than previously thought. Moreover, studies using newly available ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms18071404
更新日期:2017-06-30 00:00:00
abstract::Background: To evaluate the distribution of the transforming growth factor-beta induced (TGFBI) corneal dystrophies in a multi-ethnic population in Singapore, and to present the different phenotypes with the same genotype. Methods: This study included 32 patients. Slit lamp biomicroscopy was performed for each patient...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms22031230
更新日期:2021-01-27 00:00:00
abstract::One of the most challenging efforts in drug delivery is the targeting of the eye. The eye structure and barriers render this organ poorly permeable to drugs. Quite recently the entrance of nanoscience in ocular drug delivery has improved the penetration and half-life of drugs, especially in the anterior eye chamber, w...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15011590
更新日期:2014-01-22 00:00:00
abstract::Tea (Camellia sinensis (L.) O. Kuntze), one of the main crops in China, is high in various bioactive compounds including flavonoids, catechins, caffeine, theanine, and other amino acids. C. sinensis is also known as an accumulator of fluoride (F), and the bioactive compounds are affected by F, however, the mechanism r...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20020237
更新日期:2019-01-09 00:00:00
abstract::The consumption of carotenoid-rich vegetables such as tomatoes and tomato sauces is associated with reduced risk of several chronic diseases. The predominant carotenoids in tomato products are in the (all-E) configuration, but (Z) isomers can be formed during thermal processing. The effect of cooking time (15, 30, 45 ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms16059588
更新日期:2015-04-28 00:00:00
abstract::The effects of acidic polysaccharides purified from Gastrodia rhizome on blood pressure and serum lipid levels in spontaneously hypertensive rats (SHR) fed a high-fat diet were investigated. Acidic polysaccharides were purified from crude polysaccharides by DEAE-Sepharose CL-6B. Thirty-six male SHR were randomly divid...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms13010698
更新日期:2012-01-01 00:00:00
abstract::Vascular cognitive impairment (VCI) is the second most common cause of cognitive deficit after Alzheimer's disease. Since VCI patients represent an important target population for prevention, an ongoing effort has been made to elucidate the pathogenesis of this disorder. In this review, we summarize the information fr...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms20102405
更新日期:2019-05-15 00:00:00
abstract::The human peptide GHK (glycyl-l-histidyl-l-lysine) has multiple biological actions, all of which, according to our current knowledge, appear to be health positive. It stimulates blood vessel and nerve outgrowth, increases collagen, elastin, and glycosaminoglycan synthesis, as well as supports the function of dermal fi...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms19071987
更新日期:2018-07-07 00:00:00
abstract::Although the introduction of antibiotics in medicine has resulted in one of the most successful events and in a major breakthrough to reduce morbidity and mortality caused by infectious disease, response to these agents is not always predictable, leading to differences in their efficacy, and sometimes to the occurrenc...
journal_title:International journal of molecular sciences
pub_type: 杂志文章,评审
doi:10.3390/ijms21175975
更新日期:2020-08-19 00:00:00
abstract::Drought stress is the major abiotic factor threatening maize (Zea mays L.) yield globally. Therefore, revealing the molecular mechanisms fundamental to drought tolerance in maize becomes imperative. Herein, we conducted a comprehensive comparative analysis of two maize inbred lines contrasting in drought stress tolera...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19103225
更新日期:2018-10-18 00:00:00
abstract::It is widely accepted that chronic inflammation initiates and promotes carcinogenesis and tumor progression in various cell types. However, this paradigm has not been comprehensively investigated in melanoma. To investigate the effects of chronic inflammation on the progression of melanoma, we established a murine inf...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20010169
更新日期:2019-01-04 00:00:00
abstract::Impaired NO-cGMP signaling has been linked to several neurological disorders. NO-sensitive guanylyl cyclase (NO-GC), of which two isoforms-NO-GC1 and NO-GC2-are known, represents a promising drug target to increase cGMP in the brain. Drug-like small molecules have been discovered that work synergistically with NO to s...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms19082313
更新日期:2018-08-07 00:00:00
abstract::The main objective of this study is to explore the influence of ion composition on the trans-membrane potential across the ion exchange membrane (IEM), and thus offers a reference for the deep insight of "reverse electrodialysis heat engine" running in the composite systems. In comparison to the natural system (river ...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20235860
更新日期:2019-11-22 00:00:00
abstract::Asymmetric dimethylarginine (ADMA) is an endogenous nitric oxide synthase (NOS) inhibitor known as a mediator of endothelial dysfunction and atherosclerosis. Circulating ADMA levels are correlated with cardiovascular risk factors such as hypercholesterolemia, arterial hypertension, diabetes mellitus, hyperhomocysteine...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms15046391
更新日期:2014-04-15 00:00:00
abstract::Understanding the role of substituents is of great importance for the preparation of novel phenolic compounds with enhanced antioxidative properties. In this work, the antioxidative activity of isoflavonoid derivatives with different substituents placed at the C2 position was determined by density functional theory (D...
journal_title:International journal of molecular sciences
pub_type: 杂志文章
doi:10.3390/ijms20020397
更新日期:2019-01-18 00:00:00