Abstract:
BACKGROUND:Surface-enhanced Raman scattering (SERS) is a powerful light scattering technique that can be used for sensitive immunoassay development and cell labeling. A major obstacle to using SERS is the complexity of fabricating SERS probes since they require nanoscale characterization and optical uniformity. The light scattering response of SERS probes may also be modulated by the substrate used for SERS analysis. A typical SERS substrate such as quartz can be expensive. Polystyrene is a cheaper substrate option but can decrease the SERS response due to interfering Raman emission peaks and high background fluorescence. The goal of this research is to develop an optimized process for fabricating Raman-labeled nanoparticles for a SERS-based immunoassay on a polystyrene substrate. RESULTS:We have developed a method for fabricating SERS nanoparticle probes for use in a light scattering immunoassay on a polystyrene substrate. The light scattering profile of both spherical gold nanoparticle and gold nanorod SERS probes were characterized using Raman spectroscopy and optical absorbance spectroscopy. The effects of substrate interference and autofluorescence were reduced by selecting a Raman reporter with a strong light scattering response in a spectral region where interfering substrate emission peaks are minimized. Both spherical gold nanoparticles and gold nanorods SERS probes used in the immunoassay were detected at labeling concentrations in the low pM range. This analytical sensitivity falls within the typical dynamic range for direct labeling of cell-surface biomarkers using SERS probes. CONCLUSION:SERS nanoparticle probes were fabricated to produce a strong light scattering signal despite substrate interference. The optical extinction and inelastic light scattering of these probes was detected by optical absorbance spectroscopy and Raman spectroscopy, respectively. This immunoassay demonstrates the feasibility of analyzing strongly enhanced Raman signals on polystyrene, which is an inexpensive yet non-ideal Raman substrate. The assay sensitivity, which is in the low pM range, suggests that these SERS probe particles could be used for Raman labeling of cell or tissue samples in a polystyrene tissue culture plate. With continued development, this approach could be used for direct labeling of multiple cell surface biomarkers on strongly interfering substrate platforms.
journal_name
J Biol Engjournal_title
Journal of biological engineeringauthors
Israelsen ND,Wooley D,Hanson C,Vargis Edoi
10.1186/s13036-015-0023-ysubject
Has Abstractpub_date
2016-01-07 00:00:00pages
2issn
1754-1611pii
23journal_volume
10pub_type
杂志文章abstract:UNLABELLED: BACKGROUND:Biohydrogen from cyanobacteria has attracted public interest due to its potential as a renewable energy carrier produced from solar energy and water. Anabaena siamensis TISTR 8012, a novel strain isolated from rice paddy field in Thailand, has been identified as a promising cyanobacterial strain...
journal_title:Journal of biological engineering
pub_type: 杂志文章
doi:10.1186/1754-1611-6-19
更新日期:2012-10-10 00:00:00
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journal_title:Journal of biological engineering
pub_type: 杂志文章
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更新日期:2015-03-01 00:00:00
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journal_title:Journal of biological engineering
pub_type: 杂志文章
doi:10.1186/1754-1611-4-7
更新日期:2010-06-09 00:00:00
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journal_title:Journal of biological engineering
pub_type: 杂志文章,评审
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更新日期:2020-05-06 00:00:00
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journal_title:Journal of biological engineering
pub_type: 杂志文章
doi:10.1186/1754-1611-6-18
更新日期:2012-09-14 00:00:00
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journal_title:Journal of biological engineering
pub_type: 杂志文章
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journal_title:Journal of biological engineering
pub_type: 杂志文章
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journal_title:Journal of biological engineering
pub_type: 杂志文章
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更新日期:2018-12-04 00:00:00
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pub_type: 杂志文章
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更新日期:2019-02-07 00:00:00
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pub_type: 杂志文章
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更新日期:2019-06-28 00:00:00
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journal_title:Journal of biological engineering
pub_type: 杂志文章,评审
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更新日期:2017-12-13 00:00:00
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journal_title:Journal of biological engineering
pub_type: 杂志文章,评审
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journal_title:Journal of biological engineering
pub_type: 杂志文章,评审
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更新日期:2017-06-29 00:00:00
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journal_title:Journal of biological engineering
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Journal of biological engineering
pub_type: 杂志文章,评审
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更新日期:2019-01-18 00:00:00
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pub_type: 杂志文章
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更新日期:2019-10-29 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of biological engineering
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of biological engineering
pub_type: 杂志文章,评审
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更新日期:2020-09-09 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
doi:10.1186/1754-1611-5-12
更新日期:2011-09-20 00:00:00
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pub_type: 杂志文章
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更新日期:2020-01-13 00:00:00
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更新日期:2009-01-02 00:00:00
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journal_title:Journal of biological engineering
pub_type: 杂志文章
doi:10.1186/1754-1611-6-22
更新日期:2012-11-13 00:00:00