Abstract:
:Human iPS cells hold great promise for disease modeling and treatment of degenerative disorders including muscular dystrophies. Although a few research groups have used them for skeletal muscle differentiation, most were based on gene over-expression or long-term mesenchymal differentiation and retrospective identification of myogenic cells. Therefore, this study was aimed to generate a knock-in reporter human iPS cell line for MYF5, as an early myogenic specification gene, to allow prospective identification and purification of myogenic progenitors from human iPS cells. By using a CRISPR/Cas9 double nickase strategy, a 2A-GFP reporter was inserted before the stop codon of the MYF5 gene using homologous recombination. This approach allowed for highly efficient in-frame targeting of MYF5 in human iPS cells. Furthermore, in order to prove the reporter function, endogenous MYF5 expression was induced using a novel dead Cas9-VP160 transcriptional activator. Induced clones demonstrated appropriate MYF5-GFP co-expression. Finally, to confirm the differentiation potential, reporter human iPS clones were differentiated through embryoid body method and MYF5-GFP(+) myogenic cells were sorted and characterized. These data provides valuable guidelines for generation of knock-in reporter human iPS cell lines for myogenic genes which can be used for disease modeling, drug screening, gene correction and future in vivo applications.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wu J,Hunt SD,Xue H,Liu Y,Darabi Rdoi
10.1038/srep18759subject
Has Abstractpub_date
2016-01-05 00:00:00pages
18759issn
2045-2322pii
srep18759journal_volume
6pub_type
杂志文章abstract::The aetiology of septic acute kidney injury (AKI) is not completely elucidated. Early identification of AKI in septic patients is considered to improve survival rate since it allows rapid treatment onset. We evaluated clinical, haematological, urinary, B-mode, spectral Doppler, and contrast-enhanced ultrasound variabl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65902-4
更新日期:2020-06-02 00:00:00
abstract::Sixty-six million years ago, an asteroid approximately 9 km in diameter hit the hydrocarbon- and sulfur-rich sedimentary rocks in what is now Mexico. Recent studies have shown that this impact at the Yucatan Peninsula heated the hydrocarbon and sulfur in these rocks, forming stratospheric soot and sulfate aerosols and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14199-x
更新日期:2017-11-09 00:00:00
abstract::Tumor microenvironment favors tumor cells to promote their growth and metastasis such as migration, invasion, and angiogenesis. IL-1β, one of the inflammatory cytokines released from myeloid cells in tumor microenvironment, plays an important role in development and progress of tumor. The activation of inflammasome is...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48794-x
更新日期:2019-08-22 00:00:00
abstract::Coleoid cephalopods have an elaborate camera eye whereas nautiloids have primitive pinhole eye without lens and cornea. The Nautilus pinhole eye provides a unique example to explore the module of lens formation and its evolutionary mechanism. Here, we conducted an RNA-seq study of developing eyes of Nautilus and pygmy...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01432
更新日期:2013-01-01 00:00:00
abstract::We report evidence of the transition from n- to p-type conduction of InN with increasing Mg dopant concentration by using photoconductivity (PC) measurement at room temperature. This transition is depicted as a conversion from negative to positive PC under above-bandgap optical excitation. The n- to p-type transition ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04371
更新日期:2014-03-13 00:00:00
abstract::It is generally assumed that an investment into cognitive abilities and their associated cost is particularly beneficial for long-lived species, as a prolonged lifespan allows to recoup the initial investment. However, ephemeral organisms possess astonishing cognitive abilities too. Invertebrates, for example, are cap...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51652-5
更新日期:2019-10-23 00:00:00
abstract::Quantum cryptography is commonly used to generate fresh secure keys with quantum signal transmission for instant use between two parties. However, research shows that the relatively low key generation rate hinders its practical use where a symmetric cryptography component consumes the shared key. That is, the security...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep46302
更新日期:2017-04-13 00:00:00
abstract::Statistical learning is a key mechanism for detecting regularities from a variety of sensory inputs. Precocial newborn domestic chicks provide an excellent model for (1) exploring unsupervised forms of statistical learning in a comparative perspective, and (2) elucidating the ecological function of statistical learnin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72090-8
更新日期:2020-09-15 00:00:00
abstract::The prognostic factors for survival among patients with secondary osteosarcoma remain unclear. The aim of this study was to develop a practical nomogram for predicting cancer-specific survival (CSS) in patients with osteosarcoma as a secondary malignancy. The surveillance, epidemiology, and end results database was us...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69740-2
更新日期:2020-07-30 00:00:00
abstract::A versatile method based on Raman microscopy was developed to follow the degradation of iron carboxylate Metal Organic Framework (MOF) nano- or micro-particles in simulated body fluid (phosphate buffer). The analysis of both the morphology and chemical composition of individual particles, including observation at diff...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13323-1
更新日期:2017-10-13 00:00:00
abstract::Microtubule affinity regulating kinase 4 (MARK4) becomes a unique anti-cancer drug target as its overexpression is responsible for different types of cancers. In quest of novel, effective MARK4 inhibitors, some acridone derivatives were synthesized, characterized and evaluated for inhibitory activity against human MAR...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-38217-8
更新日期:2019-02-08 00:00:00
abstract::Azoles are antifungal drugs used to treat fungal infections such as candidiasis in humans. Their extensive use has led to the emergence of drug resistance, complicating antifungal therapy for yeast infections in critically ill patients. Combination therapy has become popular in clinical practice as a potential strateg...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17070
更新日期:2015-11-23 00:00:00
abstract::Urinary incontinence of idiopathic nature is a common complication of bladder cancer, yet, the mechanisms underlying changes in bladder contractility associated with cancer are not known. Here by using tensiometry on detrusor smooth muscle (DSM) strips from normal rats and rats with bladder cancer induced by known uro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-76653-7
更新日期:2020-11-12 00:00:00
abstract::In this study, genomic DNA was screened from Halobacillus andaensis NEAU-ST10-40T by selection in Escherichia coli KNabc lacking three major Na+/H+ antiporters. One gene designated upf0118 exhibiting Na+(Li+)/H+ antiport activity was finally cloned. Protein alignment showed that UPF0118 shares the highest identity of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45936
更新日期:2017-04-04 00:00:00
abstract::Referential pointing is a characteristically human behavior, which involves moving a finger through space to direct an addressee towards a desired mental state. Planning this type of action requires an interface between sensorimotor and conceptual abilities. A simple interface could supplement spatially-guided motor r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04442-w
更新日期:2017-06-27 00:00:00
abstract::Uterosacral ligaments (USLs) provide structural support to the female pelvic floor, and a loss of USL structural integrity or biomechanical function may induce pelvic organ prolapse (POP). Alterations in extracellular matrix composition and organization dictate USL mechanical function. Changes in USL microstructure an...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-64192-0
更新日期:2020-04-30 00:00:00
abstract::Liquid-ordered lipid domains represent a lateral inhomogeneity in cellular membranes. These domains have elastic and physicochemical properties different from those of the surrounding membrane. In particular, their thickness exceeds that of the disordered membrane. Thus, elastic deformations arise at the domain bounda...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61110-2
更新日期:2020-03-05 00:00:00
abstract::Phlebotonics' effects were evaluated to reduce time-to-stop bleeding and anal irritation in 130 patients who complained of hemorrhoidal disease (HD); bleeding and pain after hemorrhoidectomy (31 patients) and hemorrhoidal thrombosis (34 patients) in the short time. Sixty patients were randomized to receive the routine...
journal_title:Scientific reports
pub_type: 杂志文章,随机对照试验
doi:10.1038/s41598-020-64772-0
更新日期:2020-05-14 00:00:00
abstract::Previous observational studies supported a positive association of glycated hemoglobin A1c (HbA1c) level with serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C). However, the causal relationship between HbA1c and either one of ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-41076-6
更新日期:2019-04-02 00:00:00
abstract::To date, there is only scarce evidence for a considerable association of subjective and objective stress measures, which might be attributable to method bias (e.g., confounding) and/or asynchrony of their temporal changes. To validate different subjective stress measures by a physiological measure of long-term stress ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-37526-2
更新日期:2019-01-31 00:00:00
abstract::In Duchenne muscular dystrophy, progressive loss of muscle tissue is accompanied by fibrosis, chronic inflammation and reduced muscle regenerative capacity. Although much is known about the development of fibrosis and chronic inflammation in muscular dystrophy, less is known about how they are mechanistically linked t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24708
更新日期:2016-05-31 00:00:00
abstract::We theoretically investigate the mechanism of ferroelectric switching via interlayer shear in 3R MoS2 using first principles and lattice dynamics calculations. First principle calculations show the prominent anharmonic coupling of the infrared inactive interlayer shear and the infrared active phonons. The nonlinear co...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50293-y
更新日期:2019-10-17 00:00:00
abstract::During our research, we explored a novel way to represent subwavelength imaging and derived a transmission equation to explicate the FP (Fabry-Pérot) resonance phenomena. Subsequently, using analysis and observation, we performed deep-subwavelength imaging. Both numerically and experimentally, imaging with super-resol...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67409-4
更新日期:2020-07-01 00:00:00
abstract::Breast cancer is a heterogeneous disease consisting of several subtypes. Among these subtypes, triple negative breast cancer is particularly difficult to treat. This is due to a lack of understanding of the mechanisms behind the disease, and consequently a lack of druggable targets. PAK4 plays critical roles in cell s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep42555
更新日期:2017-02-15 00:00:00
abstract::Laser Capture Microdissection (LCM) is a powerful tool to isolate and study gene expression pattern of desired and less accessible cells or tissues from a heterogeneous population. Existing LCM-based methods fail to obtain high quality RNA including small RNAs from small microdissected plant tissue and therefore, are ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21577
更新日期:2016-02-10 00:00:00
abstract::In this study, we evaluated the sink and source of the surface water along the PRE using a mixing model method with salinity as tracer. The observational data showed that the decreasing of dissolved inorganic nitrogen (DIN) did not closely follow the physical mixing lines of freshwater and modified seawater. In the we...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep36638
更新日期:2016-11-11 00:00:00
abstract::We describe a new reproducibility-optimization method ROPECA for statistical analysis of proteomics data with a specific focus on the emerging data-independent acquisition (DIA) mass spectrometry technology. ROPECA optimizes the reproducibility of statistical testing on peptide-level and aggregates the peptide-level c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05949-y
更新日期:2017-07-19 00:00:00
abstract::Uterus transplantation (UTx) is a potential option for women with uterine factor infertility to have a child. The clinical features indicating irreversible rejection of the uterus are unknown. In our experimental series of allogeneic UTx in cynomolgus macaques, six female macaques were retrospectively examined, which ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70914-1
更新日期:2020-08-17 00:00:00
abstract::Although geological and modelling evidence indicate that the last glacial inception in North America was in NE Canada, little is known about the glacial response of the nearby western Greenland Ice Sheet (GIS) during the glacial advance of marine oxygen isotope stage 4 (MIS4). Our multi-proxy study of a marine sedimen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51983-3
更新日期:2019-10-30 00:00:00
abstract::Synapses are an early pathological target in many neurodegenerative diseases ranging from well-known adult onset conditions such as Alzheimer and Parkinson disease to neurodegenerative conditions of childhood such as spinal muscular atrophy (SMA) and neuronal ceroid lipofuscinosis (NCLs). However, the reasons why syna...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12603-0
更新日期:2017-09-29 00:00:00