Abstract：
:Interleukin (IL)-10, an anti-inflammatory cytokine, is expressed in the brain and can inhibit microglial activation. Herein, we utilized lipopolysaccharide (LPS)-induced inflammatory Parkinson's disease (PD) cell model to determine whether microglia and astrocytes are necessary targets for IL-10 neuroprotection. Primary ventral mesencephalic (VM) cultures with different composition of neurons, microglia and astrocytes were prepared. The cells were exposed to IL-10 (15, 50 or 150 ng/mL) 1 h prior to LPS (50 ng/mL) treatment. LPS induced dopaminergic and non-dopaminergic neuronal loss in VM cultures, VM neuron-enriched cultures, and neuron-microglia co-cultures, but not in neuron-astrocyte co-cultures. IL-10 reduced LPS-induced neuronal loss particularly in single VM neuron cultures. Pro-inflammatory mediators (TNF-α, IL-1β, inducible nitric oxide synthase and cyclooxygenase-2) were upregulated in both neuron-microglia and neuron-astrocyte co-cultures by LPS. In contrast, neurotrophic factors (brain-derived neurotrophic factor, insulin-like growth factor-1 or glial cell-derived neurotrophic factor) were downregulated in neuron-microglia co-cultures, but upregulated in neuron-astrocyte co-cultures by LPS. IL-10 reduced both the increase in production of the pro-inflammatory mediators and the decrease in production of the neurotrophic factors induced by LPS. These results suggest that astrocytes can balance LPS neurotoxicity by releasing more neurotrophic factors and that IL-10 exerts neuroprotective property by an extensive action including direct on neurons and indirect via inhibiting microglial activation.
journal_name
Int J Mol Scijournal_title
International journal of molecular sciencesauthors
Zhu Y,Chen X,Liu Z,Peng YP,Qiu YHdoi
10.3390/ijms17010025subject
Has Abstractpub_date
2015-12-28 00:00:00issue
1issn
1422-0067pii
ijms17010025journal_volume
17pub_type
杂志文章abstract：:The prognosis of patients with pancreatic cancer remains poor; only patients with small tumors and complete resection have a chance of a complete cure. Pancreatic cancer responds poorly to conventional therapies, including chemotherapy and irradiation. Snail is a transcription factor that has been associated with anti...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,收录出版
doi：10.3390/ijms11124891
更新日期：2010-01-01 00:00:00
abstract：:In homology-directed repair, mediated knock-in single-stranded oligodeoxynucleotides (ssODNs) can be used as a homologous template and present high efficiency, but there is still a need to improve efficiency. Previous studies have mainly focused on controlling double-stranded break size, ssODN stability, and the DNA r...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21113747
更新日期：2020-05-26 00:00:00
abstract：:Melatonin is an indoleamine produced from the amino acid l-tryptophan, whereas metabolites of melatonin are known as kynuramines. One of the best-known kynuramines is N¹-acetyl-N¹-formyl-5-methoxykynuramine (AFMK). Melatonin has attracted scientific attention as a potent antioxidant and protector of tissue against oxi...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2017-05-08 00:00:00
abstract：:Chemerin, a novel adipokine, has been associated with metabolic, inflammatory, and atherosclerotic diseases. We aimed to determine the genetic basis of chemerin levels by conducting a genome-wide association study (GWAS) and to investigate the role of RARRES2 polymorphisms and circulating chemerin levels in the long-t...
journal_title：International journal of molecular sciences
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更新日期：2019-03-07 00:00:00
abstract：:A tight junction (TJ) makes a physical barrier in the epidermal cells of skin. Ultraviolet (UV) light may disrupt the TJ barrier, but the mechanism has not been well clarified. Weak UVB (5 mJ/cm2) caused mislocalization of claudin-1 (CLDN1), a component of the TJ strand, and disruption of TJ barrier in human keratinoc...
journal_title：International journal of molecular sciences
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abstract：:Background: Gastric glands grow and cells reach differentiation at weaning in rats. By considering that early weaning (EW) can affect the timing of development, we aimed to compare molecular and cellular markers of differentiation in pups and adults. Methods: Wistar rats were separated into suckling-control (S) and EW...
journal_title：International journal of molecular sciences
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journal_title：International journal of molecular sciences
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更新日期：2019-09-20 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2019-08-16 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2020-10-03 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18102064
更新日期：2017-09-27 00:00:00
abstract：:The authors wish to make the following corrections to this paper [...]. ...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,已发布勘误
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更新日期：2020-06-26 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms14024005
更新日期：2013-02-18 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2016-04-15 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21010170
更新日期：2019-12-25 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12106796
更新日期：2011-01-01 00:00:00
abstract：:Cytomegalovirus (CMV) infection after hematopoietic stem cell transplantation (HSCT) is one of the critical infectious complications related to host immune recovery. The spectrum of CMV infection is quite extensive, from asymptomatic CMV reactivation presenting mainly as CMV DNAemia to fatal CMV diseases involving gut...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2019-05-30 00:00:00
abstract：:(R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Analysis of Western bl...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms13032717
更新日期：2012-01-01 00:00:00
abstract：:Chemerin (CHEM) may act as an important link integrating energy homeostasis and reproductive functions of females, and its actions are mediated by three receptors: chemokine-like receptor 1 (CMKLR1), G protein-coupled receptor 1 (GPR1), and C-C motif chemokine receptor-like 2 (CCRL2). The aim of the current study was ...
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更新日期：2019-08-09 00:00:00
abstract：:Oxidative damage (production and localization of reactive oxygen species) and related response mechanisms (activity of antioxidant enzymes), and induction of Heat Shock Protein 70 expression, have been studied in the toxi-tolerant liverwort Conocephalum conicum (Marchantiales) in response to cadmium stress using two c...
journal_title：International journal of molecular sciences
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更新日期：2020-09-04 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2019-09-29 00:00:00
abstract：:Alpha-synuclein is considered the major pathological protein associated with Parkinson's disease, but there is still no effective immunotherapy which targets alpha-synuclein. In order to create a safer and more effective therapy against PD, we are targeting an epitope of alpha-synuclein rather than full-length alpha-s...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
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更新日期：2019-05-11 00:00:00
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journal_title：International journal of molecular sciences
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更新日期：2017-12-10 00:00:00
abstract：:Contrast-induced acute kidney injury (CI-AKI) is the third most common cause of hospital associated kidney damage. Potential mechanisms of CI-AKI may involve diminished renal hemodynamics, inflammatory responses, and direct cytotoxicity. The hypothesis for this study is that diatrizoic acid (DA) induces direct cytotox...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20174074
更新日期：2019-08-21 00:00:00
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journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2020-09-15 00:00:00
abstract：:A major hallmark of diabetes is a constant high blood glucose level (hyperglycaemia), resulting in endothelial dysfunction. Transient or prolonged hyperglycemia can cause diabetic vasculopathy, a secondary systemic damage. C-Peptide is a product of cleavage of proinsulin by a serine protease that occurs within the pan...
journal_title：International journal of molecular sciences
pub_type： 杂志文章,评审
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更新日期：2020-01-18 00:00:00
abstract：:Skeletal muscle has a remarkable plasticity, and its phenotype is strongly influenced by hormones, transcription factors, and physical activity. However, whether skeletal phenotype can be oriented or not during early embryonic stages has never been investigated. Here, we report that pyruvate as the only source of carb...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms21197203
更新日期：2020-09-29 00:00:00
abstract：:2-Bromopropane (2-BP) is used as an alternative to ozone-depleting cleaning solvents. Previously, we reported that 2-BP has cytotoxic effects on mouse blastocysts and is associated with defects in subsequent development. In the present work, we show that 2-BP induces apoptosis in the inner cell mass of mouse blastocys...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms12084991
更新日期：2011-01-01 00:00:00
abstract：:Primary biliary cholangitis (PBC) is an immune-mediated cholestatic disease. Vitamin D receptor (VDR)-dependent signaling constrains an inflammatory response by targeting the miRNA155-SOCS1 (suppressor of cytokine signaling 1) axis. The VDR-miRNA155-SOCS1 pathway was investigated in the context of the autoimmune respo...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms18020289
更新日期：2017-01-29 00:00:00
abstract：:It has been well established that hypoxia significantly increases both cellular and tumor resistance to ionizing radiation. Hypoxia associated radiation resistance has been known for some time but there has been limited success in sensitizing cells to radiation under hypoxic conditions. These studies show that, when i...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms19082228
更新日期：2018-07-30 00:00:00
abstract：:In this paper, nanofibrous membranes based on chitosan (CS), poly (vinyl alcohol) (PVA) and graphene oxide (GO) composites, loaded with antibiotic drugs, such as Ciprofloxacin (Cip) and Ciprofloxacin hydrochloride (CipHcl) were prepared via the electrospinning technique. The uniform and defect-free CS/PVA nanofibers w...
journal_title：International journal of molecular sciences
pub_type： 杂志文章
doi：10.3390/ijms20184395
更新日期：2019-09-06 00:00:00
