Abstract:
:Extracellular vesicles (EVs) secreted by cells present an attractive strategy for developing new therapies, but progress in the field is limited by several issues: The quality of the EVs varies with the type and physiological status of the producer cells; protocols used to isolate the EVs are difficult to scale up; and assays for efficacy are difficult to develop. In the present report, we have addressed these issues by using human mesenchymal stem/stromal cells (MSCs) that produce EVs when incubated in a protein-free medium, preselecting the preparations of MSCs with a biomarker for their potency in modulating inflammation, incubating the cells in a chemically defined protein-free medium that provided a stable environment, isolating the EVs with a scalable chromatographic procedure, and developing an in vivo assay for efficacy of the cells in suppressing neuroinflammation after traumatic brain injury (TBI) in mice. In addition, we demonstrate that i.v. infusion of the isolated EVs shortly after induction of TBI rescued pattern separation and spatial learning impairments 1 mo later.
journal_name
Proc Natl Acad Sci U S Aauthors
Kim DK,Nishida H,An SY,Shetty AK,Bartosh TJ,Prockop DJdoi
10.1073/pnas.1522297113subject
Has Abstractpub_date
2016-01-05 00:00:00pages
170-5issue
1eissn
0027-8424issn
1091-6490pii
1522297113journal_volume
113pub_type
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