Abstract:
:High expression levels of SLFN11 correlate with the sensitivity of human cancer cells to DNA-damaging agents. However, little is known about the underlying mechanism. Here, we show that SLFN11 interacts directly with RPA1 and is recruited to sites of DNA damage in an RPA1-dependent manner. Furthermore, we establish that SLFN11 inhibits checkpoint maintenance and homologous recombination repair by promoting the destabilization of the RPA-ssDNA complex, thereby sensitizing cancer cell lines expressing high endogenous levels of SLFN11 to DNA-damaging agents. Finally, we demonstrate that the RPA1-binding ability of SLFN11 is required for its function in the DNA damage response. Our findings not only provide novel insight into the molecular mechanisms underlying the drug sensitivity of cancer cell lines expressing SLFN11 at high levels, but also suggest that SLFN11 expression can serve as a biomarker to predict responses to DNA-damaging therapeutic agents.
journal_name
EMBO Repjournal_title
EMBO reportsauthors
Mu Y,Lou J,Srivastava M,Zhao B,Feng XH,Liu T,Chen J,Huang Jdoi
10.15252/embr.201540964subject
Has Abstractpub_date
2016-01-01 00:00:00pages
94-109issue
1eissn
1469-221Xissn
1469-3178pii
embr.201540964journal_volume
17pub_type
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