Abstract：
:Generation of multiple genomic alterations is currently a time consuming process. Here, a method was established that enables highly efficient and simultaneous deletion of multiple genes in Escherichia coli. A temperature sensitive plasmid containing arabinose inducible lambda Red recombineering genes and a rhamnose inducible flippase recombinase was constructed to facilitate fast marker-free deletions. To further speed up the procedure, we integrated the arabinose inducible lambda Red recombineering genes and the rhamnose inducible FLP into the genome of E. coli K-12 MG1655. This system enables growth at 37 °C, thereby facilitating removal of integrated antibiotic cassettes and deletion of additional genes in the same day. Phosphorothioated primers were demonstrated to enable simultaneous deletions during one round of electroporation. Utilizing these methods, we constructed strains in which four to seven genes were deleted in E. coli W and E. coli K-12. The growth rate of an E. coli K-12 quintuple deletion strain was significantly improved in the presence of high concentrations of acetate and NaCl. In conclusion, we have generated a method that enables efficient and simultaneous deletion of multiple genes in several E. coli variants. The method enables deletion of up to seven genes in as little as seven days.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Jensen SI,Lennen RM,Herrgård MJ,Nielsen ATdoi
10.1038/srep17874subject
Has Abstractpub_date
2015-12-08 00:00:00pages
17874issn
2045-2322pii
srep17874journal_volume
5pub_type
杂志文章abstract：:Human milk delivers an array of bioactive components that safeguard infant growth and development and maintain healthy gut microbiota. Milk fat globule membrane (MFGM) is a biologically functional fraction of milk increasingly linked to beneficial outcomes in infants through protection from pathogens, modulation of th...
journal_title：Scientific reports
pub_type： 杂志文章,随机对照试验
doi：10.1038/s41598-019-47953-4
更新日期：2019-08-12 00:00:00
abstract：:The dispersal of animals from their birth place has profound effects on the immediate survival and longer-term persistence of populations. Molecular studies have estimated that bumblebee colonies can be established many kilometers from their queens' natal nest site. However, little is known about when and how queens d...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-40355-6
更新日期：2019-03-20 00:00:00
abstract：:This study investigated Staphylococcus aureus carriage in patients with microbial keratitis (MK). 215 patients with MK, 60 healthy controls and 35 patients with rheumatoid arthritis (RA) were included. Corneal scrapes were collected from patients with MK. Conjunctival, nasal and throat swabs were collected from the no...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-75821-z
更新日期：2020-10-29 00:00:00
abstract：:A single radiation-induced superoxide ion [Formula: see text] has been observed for the first time in metal oxides. This structural defect has been revealed in fast-neutron-irradiated (6.9×1018 n/cm2) corundum (α-Al2O3) single crystals using the EPR method. Based on the angular dependence of the EPR lines at the magne...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-72958-9
更新日期：2020-09-28 00:00:00
abstract：:Abiotic synthesis of biomolecules is an essential step for the chemical origin of life. Many attempts have succeeded in synthesizing biomolecules, including amino acids and nucleobases (e.g., via spark discharge, impact shock, and hydrothermal heating), from reduced compounds that may have been limited in their availa...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-66112-8
更新日期：2020-06-08 00:00:00
abstract：:SERS spectra excited at 785 nm of the bacteria Chlamydia trahomatis (elementary bodies, EB) and Neisseria gonorrheoae, the causative pathogens for the two most common sexually transmitted diseases (STD), chlamydia and gonorrhea, respectively, are reported. Although both are Gram-negative bacteria, the SERS signatures ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23562-5
更新日期：2018-03-26 00:00:00
abstract：:A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper. ...
journal_title：Scientific reports
pub_type： 已发布勘误
doi：10.1038/s41598-018-28651-z
更新日期：2018-07-05 00:00:00
abstract：:A major challenge to routine non-invasive, nanoscale magnetic imaging is the development of Hall sensors that are stable under ambient conditions and retain low minimum detectable fields down to nanoscale dimensions. To address these issues we have fabricated and characterised chemical vapour deposition (CVD) graphene...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50823-8
更新日期：2019-10-08 00:00:00
abstract：:Sharp increase in macromolecular crowding induces abnormal chromatin compaction in the cell nucleus, suggesting its non-negligible impact on chromatin structure and function. However, the details of the crowding-induced chromatin compaction remain poorly understood. In this work, we present a computer simulation study...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-23753-0
更新日期：2018-04-03 00:00:00
abstract：:Arrays of sub-wavelength, sub-10 nm air-gap plasmonic ring resonators are fabricated using nanoimprinting. In near infra-red (NIR) range, the resonator supports a single dipole mode which is excited and identified via simple normal illumination and explored through transmission measurements. By controlling both latera...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22305
更新日期：2016-02-29 00:00:00
abstract：:III-nitride materials have been linked with a vast number of exciting applications from power electronics to solar cells. Herein, polycrystalline InN, GaN and systematically controlled InxGa1-xN composite thin films are fabricated on FTO glass by a facile, low-cost and scalable aerosol assisted chemical vapor depositi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-38882-3
更新日期：2019-02-19 00:00:00
abstract：:Hepatocyte spheroids are useful models for mimicking liver phenotypes in vitro because of their three-dimensionality. However, the lack of a biomaterial platform which allows the facile manipulation of spheroid cultures on a large scale severely limits their application in automated high-throughput drug safety testing...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61699-4
更新日期：2020-03-16 00:00:00
abstract：:Spin-polarized triangular graphene nanoflakes (t-GNFs) serve as ideal building blocks for the long-desired ferromagnetic graphene superlattices, but they are always assembled to planar structures which reduce its mechanical properties. Here, by joining t-GNFs in a spiral way, we propose one-dimensional graphene spiral...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep05699
更新日期：2014-07-16 00:00:00
abstract：:Biofilms are complex microbial communities that present serious contamination risks to our environment and health. In this study, atmospheric air plasma and airborne acoustic ultrasound technology were applied to inactivate Escherichia coli and Listeria innocua biofilms. Both technologies were efficient in controlling...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81977-z
更新日期：2021-01-27 00:00:00
abstract：:Solid stress is a biomechanical abnormality of the tumor microenvironment that plays a crucial role in tumor progression. When it is applied to cancer cells, solid stress hinders their proliferation rate and promotes cancer cell invasion and metastatic potential. However, the underlying mechanisms of how it is implica...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37425-6
更新日期：2019-01-30 00:00:00
abstract：:Brood is a very valuable part of an ant colony and behaviours increasing its number with minimum investment is expected to be favoured by natural selection. Brood theft has been well documented in ants belonging to the subfamilies Myrmicinae and Formicinae. In this study we report opportunistic brood theft in the cont...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep36166
更新日期：2016-10-31 00:00:00
abstract：:L-Asparaginase (L-asparagine aminohydrolase, E.C. 3.5.1.1) has been proven to be competent in treating Acute Lymphoblastic Leukaemia (ALL), which is widely observed in paediatric and adult groups. Currently, clinical L-Asparaginase formulations are derived from bacterial sources such as Escherichia coli and Erwinia ch...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-38094-1
更新日期：2019-02-05 00:00:00
abstract：:Comparison of kinetic and thermodynamic properties of IRP1 (iron regulatory protein1) binding to FRT (ferritin) and ACO2 (aconitase2) IRE-RNAs, with or without Mn2+, revealed differences specific to each IRE-RNA. Conserved among animal mRNAs, IRE-RNA structures are noncoding and bind Fe2+ to regulate biosynthesis rate...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-09093-5
更新日期：2017-08-17 00:00:00
abstract：:The microbial colonization of the intestine during the first months of life constitutes the most important process for the microbiota-induced host-homeostasis. Alterations in this process may entail a high-risk for disease in later life. However, the potential factors affecting this process in the infant are not well ...
journal_title：Scientific reports
pub_type： 临床试验,杂志文章
doi：10.1038/srep07417
更新日期：2014-12-10 00:00:00
abstract：:In recent years, texture analysis of medical images has become increasingly popular in studies investigating diagnosis, classification and treatment response assessment of cancerous disease. Despite numerous applications in oncology and medical imaging in general, there is no consensus regarding texture analysis workf...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-04151-4
更新日期：2017-06-22 00:00:00
abstract：:Communication between neurons involves presynaptic neurotransmitter release which can be evoked by action potentials or occur spontaneously as a result of stochastic vesicle fusion. The Ca2+-binding double C2 proteins Doc2a and -b were implicated in spontaneous and asynchronous evoked release, but the mechanism remain...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-50684-1
更新日期：2019-10-08 00:00:00
abstract：:In this paper, one kind of novel non-periodic spoof surface plasmon polaritons (SSPPs) with H-shaped cells is proposed. As we all know, the cutoff frequency exists inherently for the conventional comb-shaped SSPPs, which is a kind of periodic groove shape structures and fed by a conventional coplanar waveguide (CPW). ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-20533-8
更新日期：2018-02-06 00:00:00
abstract：:Telomere DNA ends with a single-stranded 3' overhang. Long 3' overhangs may cause aberrant DNA damage responses and accelerate telomere attrition, which is associated with cancer and aging, respectively. Genetic studies have indicated several important players in preventing 5' end hyper-resection, yet detailed knowled...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-08663-x
更新日期：2017-08-18 00:00:00
abstract：:The ZIC proteins are a family of transcription regulators with a well-defined zinc finger DNA-binding domain and there is evidence that they elicit functional DNA binding at a ZIC DNA binding site. Little is known, however, regarding domains within ZIC proteins that confer trans-activation or -repression. To address t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69917-9
更新日期：2020-08-04 00:00:00
abstract：:Lower corrected calcium (cCa) levels are associated with a better prognosis among incident dialysis patients. However, cCa frequently overestimates ionized calcium (iCa) levels. The prognostic importance of the true calcium status defined by iCa remains to be revealed. We conducted a retrospective cohort study of inci...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-61459-4
更新日期：2020-03-10 00:00:00
abstract：:Surfaces with reversible wettability have broad applications but remain challenging since the switching process is usually energy intensive and complex. In this paper, a pyramid shaped Cu2S film with hierarchical micro/nanostructures is formed on a commercial copper mesh. This film is formed by a spontaneous redox sul...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-48952-1
更新日期：2019-08-28 00:00:00
abstract：:We discuss a scheme to retrieve transient conformational molecular structure information using photoelectron angular distributions (PADs) that have averaged over partial alignments of isolated molecules. The photoelectron is pulled out from a localized inner-shell molecular orbital by an X-ray photon. We show that a t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23655
更新日期：2016-03-30 00:00:00
abstract：:Double quantum dots (DQDs) are a versatile platform for solid-state physics, quantum computation and nanotechnology. The micro-fabrication techniques commonly used to fabricate DQDs are difficult to extend to the atomic scale. Using an alternative approach, which relies on scanning tunneling microscopy and spectroscop...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-10814-z
更新日期：2017-09-07 00:00:00
abstract：:Rhodopseudomonas palustris CGA009 is a purple non-sulfur bacterium that can fix carbon dioxide (CO2) and nitrogen or break down organic compounds for its carbon and nitrogen requirements. Light, inorganic, and organic compounds can all be used for its source of energy. Excess electrons produced during its metabolic pr...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49079-z
更新日期：2019-09-02 00:00:00
abstract：:RNA editing is a molecular event that alters specific nucleotides in RNA post-transcriptionally. RNA editing has the potential to impact a variety of cellular processes and is implicated in diseases such as cancer. Yet, the precise mechanisms by which RNA editing controls cellular processes are poorly understood. Here...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23226
更新日期：2016-03-16 00:00:00
