Abstract:
:Mammalian spermatogenesis is a classic adult stems cell-dependent process, supported by the self-renewal and differentiation of spermatogonial stem cells (SSCs). However, the identification of SSCs and elucidation of their behaviors in undisturbed testis has long been a big challenge. Here, we generated a knock-in mouse model, Id4-2A-CreERT2-2A-tdTomato, which allowed us to mark Id4-expressing (Id4(+)) cells at different time points in situ and track their behaviors across distinct developmental stages during steady-state and regenerating spermatogenesis. We found that Id4(+) cells continue to produce spermatogonia, spermatocytes and sperm in mouse testis, showing they are capable of self-renewal and have differentiation potential. Consistent with these findings, ablation of Id4(+) cells in mice results in a loss of spermatogenesis. Furthermore, developmental fate mapping reveals that Id4(+) SSCs originate from neonate Id4(+) gonocytes. Therefore, our results indicate that Id4 marks spermatogonial stem cells in the mouse testis.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Sun F,Xu Q,Zhao D,Degui Chen Cdoi
10.1038/srep17594subject
Has Abstractpub_date
2015-12-01 00:00:00pages
17594issn
2045-2322pii
srep17594journal_volume
5pub_type
杂志文章abstract::Effective application of whole-cell devices in synthetic biology and biocatalysis will always require consideration of the uptake of molecules of interest into the cell. Here we demonstrate that the AlkL protein from Pseudomonas putida GPo1 is an alkane import protein capable of industrially relevant rates of uptake o...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05844
更新日期:2014-07-28 00:00:00
abstract::Although most models suggest continental Antarctica was covered by ice during the Last Glacial Maximum (LGM) it has been speculated that endemic species of soil invertebrates could have survived the Pleistocene at high elevation habitats protruding above the ice sheets. We analyzed a series of soil samples from differ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep26189
更新日期:2016-05-18 00:00:00
abstract::We assessed 1-year outcomes in patients with atrial fibrillation enrolled in the EurObservational Research Programme AF General Pilot Registry (EORP-AF), in relation to kidney function, as assessed by glomerular filtration rate (eGFR). In a cohort of 2398 patients (median age 69 years; 61% male), eGFR (ml/min/1.73 m(2...
journal_title:Scientific reports
pub_type: 杂志文章,多中心研究
doi:10.1038/srep30271
更新日期:2016-07-28 00:00:00
abstract::To evaluate crop rotation effects on maize seedling performance and its associated microbiome, maize plants were grown in the greenhouse in soils preceded by either maize, pea, soybean or sunflower. Soils originated from a replicated field experiment evaluating different four-year rotation combinations. In the greenho...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-15955-9
更新日期:2017-11-16 00:00:00
abstract::Current chemical markers based quality assessment methods largely fail to reflect intrinsic chemical complexity and multiple mechanisms of action of botanical drugs (BD). The development of novel quality markers is greatly needed. Here we propose bioactive chemical markers (BCM), defined as a group of chemo-markers th...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-02305-y
更新日期:2017-05-25 00:00:00
abstract::Cellular clearance mechanisms including the autophagy-lysosome pathway are impaired in amyotrophic lateral sclerosis (ALS). One of the most important proteins involved in the regulation of autophagy is the lysosomal Ca2+ channel Mucolipin TRP channel 1 (TRPML1). Therefore, we investigated the role of TRPML1 in a neuro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46708-5
更新日期:2019-07-24 00:00:00
abstract::Rubber dams are widely used for landscaping in urban rivers and they retain large amounts of sediments. The sediments are rich in phosphorus (P) which can cause river eutrophication. Little is known about P release in rubber dams. We investigated the potential of sediment P release by isotherm experiment in an urban r...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-59493-3
更新日期:2020-02-17 00:00:00
abstract::Green reduction of graphene oxide (GO) by phytochemicals was explored using the aqueous extract of kaffir lime peels. The research methods included preparation of extracts, preparation of GO, preparation and characterization of reduced-GO (RGO) using Fourier Transform Infrared (FTIR), X-ray diffraction (XRD), and UV-V...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57433-9
更新日期:2020-01-20 00:00:00
abstract::In order to obtain a detailed understanding of the modulation of electronic properties in nanoporous Si (np-Si) nanowires with containing ordered, nanometer-sized cylindrical pores, we propose a theoretical method to clarify the band shift and associated with the dielectric modification determined by the geometrical p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-14647-8
更新日期:2017-10-31 00:00:00
abstract::Quantum measurement using coherent superposition of intrinsic atomic states has the advantage of being absolute measurement and can form metrological standards. One example is the absolute measurement of magnetic field by monitoring the Larmor precession of atomic spins whilst another being the Ramsey type atomic cloc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep28169
更新日期:2016-06-30 00:00:00
abstract::Horseradish peroxidase (HRP) mediates efficient conversion of many phenolic contaminants and thus has potential applications for pollution control. Such potentially important applications suffer however from the fact that the enzyme becomes quickly inactivated during phenol oxidation and polymerization. The work here ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep03126
更新日期:2013-11-04 00:00:00
abstract::The voltage-gated sodium channel subtype NaV1.8 is expressed in the peripheral nervous system in primary afferent nociceptive C-fibers and is essential for noxious cold signaling. We utilized functional magnetic resonance imaging on NaV1.8-deficient (NaV1.8-/-) compared with wildtype (WT) mice to identify brain struct...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00524-x
更新日期:2017-04-03 00:00:00
abstract::Thioflavin T (ThT) is standardly used as a fluorescent marker to detect aggregation of amyloid fibrils by conventional fluorescence microscopy, including polarization resolved imaging that brings information on the orientational order of the fibrils. These techniques are however diffraction limited and cannot provide ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-12864-9
更新日期:2017-10-02 00:00:00
abstract::The sensory neurons in the olfactory epithelium (OSNs) are equipped with a large repertoire of olfactory receptors and the associated signal transduction machinery. In addition to the canonical OSNs, which express odorant receptors (ORs), the epithelium contains specialized subpopulations of sensory neurons that can d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep25507
更新日期:2016-05-05 00:00:00
abstract::The colour of oyster shells is a very diverse characteristic morphotype, forming intriguing vivid patterns both on the inside and outside of the shell. In the present study, we have identified for the first time, the presence of several porphyrins as constituents of the shell pigmentation of the Crassostrea gigas oyst...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-69133-5
更新日期:2020-07-22 00:00:00
abstract::Legionnaires' disease (LD) is an atypical pneumonia caused by the inhalation of Legionella. The methods used for the diagnosis of LD are direct culture of respiratory samples and urinary antigen detection. However, the sensitivity of culture is low, and the urinary antigen test is specific only for L. pneumophila sg1....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36924-w
更新日期:2019-01-24 00:00:00
abstract::An amendment to this paper has been published and can be accessed via a link at the top of the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-020-57570-1
更新日期:2020-01-14 00:00:00
abstract::Ultra-short proton pulses originating from laser-plasma accelerators can provide instantaneous dose rates at least 10(7)-fold in excess of conventional, continuous proton beams. The impact of such extremely high proton dose rates on A549 human lung cancer cells was compared with conventionally accelerated protons and ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32441
更新日期:2016-08-31 00:00:00
abstract::Cnidarians, in general, are long-lived organisms and hence may repeatedly encounter common pathogens during their lifespans. It remains unknown whether these early diverging animals possess some type of immunological reaction that strengthens the defense response upon repeated infections, such as that described in mor...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17425
更新日期:2015-12-02 00:00:00
abstract::Sub-gap density of states (DOS) is a key parameter to impact the electrical characteristics of semiconductor materials-based transistors in integrated circuits. Previously, spectroscopy methodologies for DOS extractions include the static methods, temperature dependent spectroscopy and photonic spectroscopy. However, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep24096
更新日期:2016-06-14 00:00:00
abstract::Coral reefs across the world's oceans are in the midst of the longest bleaching event on record (from 2014 to at least 2016). As many of the world's reefs are remote, there is limited information on how past thermal conditions have influenced reef composition and current stress responses. Using satellite temperature d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38402
更新日期:2016-12-06 00:00:00
abstract::The effect of hypoglycemia on the progression of atherosclerosis in patients with type 2 diabetes mellitus (T2DM) remains largely unknown. This is a post hoc analysis of a randomized trial to investigate the relationship between hypoglycemic episodes and changes in carotid intima-media thickness (IMT). Among 274 study...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39965
更新日期:2017-01-09 00:00:00
abstract::The ability of bacteria and fungi to communicate with each other is a remarkable aspect of the microbial world. It is recognized that volatile organic compounds (VOCs) act as communication signals, however the molecular responses by bacteria to fungal VOCs remain unknown. Here we perform transcriptomics and proteomics...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-00893-3
更新日期:2017-04-13 00:00:00
abstract::Albacore tuna (Thunnus alalunga) is an important target of tuna fisheries in the Atlantic and Indian Oceans. The commercial catch of albacore is the highest globally among all temperate tuna species, contributing around 6% in weight to global tuna catches over the last decade. The accurate assessment and management of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72369-w
更新日期:2020-09-24 00:00:00
abstract::Vitiligo is characterized by death or functional defects of epidermal melanocytes through still controversial pathogenic process. Previously, we showed that mitochondria-driven pre-senescent phenotype diminishes the capability of vitiligo melanocytes to cope with stressful stimuli. In the current study, we investigate...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13961-5
更新日期:2017-10-20 00:00:00
abstract::We examined how plant-pollinator interactions were affected by time since habitat restoration and landscape connectivity by comparing plant-pollinator networks in restored, abandoned and continuously grazed semi-natural pastures in south-central Sweden. We measured richness of flowering plants and pollinators, and loc...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-51912-4
更新日期:2019-10-29 00:00:00
abstract::Solution-based methods represent the most widespread approach used to deposit hybrid organic-inorganic perovskite films for low-cost but efficient solar cells. However, solution-process techniques offer limited control over film morphology and crystallinity, and most importantly do not allow sequential film deposition...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-33760-w
更新日期:2018-10-18 00:00:00
abstract::Generally, the length of the oxide nanowires grown by vapor phase transport is limited by the degradation of the source materials. Furthermore, the source material is used once for the nanowires growth. By exploiting the Si-Zn phase diagram, we have developed a simple methodology for the non-catalytic growth of ultral...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep01172
更新日期:2013-01-01 00:00:00
abstract::Light-inducible gene regulation has great potential for remote and noninvasive control of the fate and function of target cells. One method to achieve such control is delivery of heat shock protein (HSP) promoter-driven protein expression vectors and photothermal heaters into the cells, followed by activation by illum...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04912-1
更新日期:2017-07-05 00:00:00
abstract::In this paper, for the first time, the switchable two-color quantum dot laser has been realized considering solution process technology, which has both simultaneous and lonely lasing capability exploiting selective energy contacts. Furthermore, both channels can be modulated independently, which is a significant featu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-60859-w
更新日期:2020-03-24 00:00:00