Abstract:
:Curly, described almost a century ago, is one of the most frequently used markers in Drosophila genetics. Despite this the molecular identity of Curly has remained obscure. Here we show that Curly mutations arise in the gene dual oxidase (duox), which encodes a reactive oxygen species (ROS) generating NADPH oxidase. Using Curly mutations and RNA interference (RNAi), we demonstrate that Duox autonomously stabilizes the wing on the last day of pupal development. Through genetic suppression studies, we identify a novel heme peroxidase, Curly Su (Cysu) that acts with Duox to form the wing. Ultrastructural analysis suggests that Duox and Cysu are required in the wing to bond and adhere the dorsal and ventral cuticle surfaces during its maturation. In Drosophila, Duox is best known for its role in the killing of pathogens by generating bactericidal ROS. Our work adds to a growing number of studies suggesting that Duox's primary function is more structural, helping to form extracellular and cuticle structures in conjunction with peroxidases.
journal_name
PLoS Genetjournal_title
PLoS geneticsauthors
Hurd TR,Liang FX,Lehmann Rdoi
10.1371/journal.pgen.1005625subject
Has Abstractpub_date
2015-11-20 00:00:00pages
e1005625issue
11eissn
1553-7390issn
1553-7404pii
PGENETICS-D-15-02168journal_volume
11pub_type
杂志文章相关文献
PLoS Genetics文献大全abstract::Intercellular communication mediated by gap junction (GJ) proteins is indispensable during embryogenesis, tissue regeneration and wound healing. Here we report functional analysis of a gap junction protein, Innexin 2 (Inx2), in cell type specification during Drosophila oogenesis. Our data reveal a novel involvement of...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006542
更新日期:2017-01-23 00:00:00
abstract::Movement of the transcription machinery along a template alters DNA topology resulting in the accumulation of supercoils in DNA. The positive supercoils generated ahead of transcribing RNA polymerase (RNAP) and the negative supercoils accumulating behind impose severe topological constraints impeding transcription pro...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006754
更新日期:2017-05-02 00:00:00
abstract::House dust mites are common pests with an unusual evolutionary history, being descendants of a parasitic ancestor. Transition to parasitism is frequently accompanied by genome rearrangements, possibly to accommodate the genetic change needed to access new ecology. Transposable element (TE) activity is a source of geno...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007183
更新日期:2018-01-29 00:00:00
abstract::To understand the genetic mechanisms leading to phenotypic differentiation, it is important to identify genomic regions under selection. We scanned the genome of two chicken lines from a single trait selection experiment, where 50 generations of selection have resulted in a 9-fold difference in body weight. Analyses o...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001188
更新日期:2010-11-04 00:00:00
abstract::Genome-wide association studies (GWAS) have become increasingly common due to advances in technology and have permitted the identification of differences in single nucleotide polymorphism (SNP) alleles that are associated with diseases. However, while typical GWAS analysis techniques treat markers individually, comple...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002101
更新日期:2011-06-01 00:00:00
abstract::Skin pigment patterns are important, being under strong selection for multiple roles including camouflage and UV protection. Pigment cells underlying these patterns form from adult pigment stem cells (APSCs). In zebrafish, APSCs derive from embryonic neural crest cells, but sit dormant until activated to produce pigme...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007941
更新日期:2019-02-27 00:00:00
abstract::Genomic regions that are unusually divergent between closely related species or racial groups can be particularly informative about the process of speciation or the operation of natural selection. The two sequenced genomes of cultivated Asian rice, Oryza sativa, reveal that at least 6% of the genomes are unusually div...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0020199
更新日期:2006-11-17 00:00:00
abstract::During meiotic prophase, a structure called the synaptonemal complex (SC) assembles at the interface between aligned pairs of homologous chromosomes, and crossover recombination events occur between their DNA molecules. Here we investigate the inter-relationships between these two hallmark features of the meiotic prog...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006670
更新日期:2017-03-24 00:00:00
abstract::It is known that genetic variants can affect gene expression, but it is not yet completely clear through what mechanisms genetic variation mediate this expression. We therefore compared the cis-effect of single nucleotide polymorphisms (SNPs) on gene expression between blood samples from 1,240 human subjects and four ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002431
更新日期:2012-01-01 00:00:00
abstract::The evolutionary divergence of mitochondrial ribosomes from their bacterial and cytoplasmic ancestors has resulted in reduced RNA content and the acquisition of mitochondria-specific proteins. The mitochondrial ribosomal protein of the small subunit 34 (MRPS34) is a mitochondria-specific ribosomal protein found only i...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005089
更新日期:2015-03-27 00:00:00
abstract::Understanding cell proliferation mechanisms has been a long-lasting goal of the scientific community and specifically of cancer researchers. Previous genome-scale studies of cancer proliferation determinants have mainly relied on knockdown screens aimed to gauge their effects on cancer growth. This powerful approach h...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1003806
更新日期:2013-01-01 00:00:00
abstract::The interplay of microbiota and the human host is physiologically crucial in health and diseases. The beneficial effects of lactic acid bacteria (LAB), permanently colonizing the human intestine or transiently obtained from food, have been extensively reported. However, the molecular understanding of how LAB modulate ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008145
更新日期:2019-05-23 00:00:00
abstract::Genetic disruption of the dystrophin complex produces muscular dystrophy characterized by a fragile muscle plasma membrane leading to excessive muscle degeneration. Two genetic modifiers of Duchenne Muscular Dystrophy implicate the transforming growth factor β (TGFβ) pathway, osteopontin encoded by the SPP1 gene and l...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007070
更新日期:2017-10-24 00:00:00
abstract::Genome-wide association studies have successfully identified thousands of loci for a range of human complex traits and diseases. The proportion of phenotypic variance explained by significant associations is, however, limited. Given the same dense SNP panels, mixed model analyses capture a greater proportion of phenot...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005804
更新日期:2016-02-02 00:00:00
abstract::Neisseria meningitidis is the primary causative agent of bacterial meningitis. The genome is rich in repetitive DNA and almost 2% is occupied by a diminutive transposon called the Correia element. Here we report a bioinformatic analysis defining eight subtypes of the element with four distinct types of ends. Transcrip...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1001277
更新日期:2011-01-20 00:00:00
abstract::The major transition to eusociality required the evolution of a switch to canalize development into either a reproductive or a helper, the nature of which is currently unknown. Following predictions from the 'theory of facilitated variation', we identify sex differentiation pathways as promising candidates because of ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005952
更新日期:2016-03-31 00:00:00
abstract::Despite the well-documented role of remote enhancers in controlling developmental gene expression, the mechanisms that allocate enhancers to genes are poorly characterized. Here, we investigate the cis-regulatory organization of the locus containing the Tfap2c and Bmp7 genes in vivo, using a series of engineered chrom...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004897
更新日期:2015-01-08 00:00:00
abstract::SHANK genes code for scaffold proteins located at the post-synaptic density of glutamatergic synapses. In neurons, SHANK2 and SHANK3 have a positive effect on the induction and maturation of dendritic spines, whereas SHANK1 induces the enlargement of spine heads. Mutations in SHANK genes have been associated with auti...
journal_title:PLoS genetics
pub_type: 杂志文章,meta分析
doi:10.1371/journal.pgen.1004580
更新日期:2014-09-04 00:00:00
abstract::Cockayne syndrome (CS) is a devastating progeria most often caused by mutations in the CSB gene encoding a SWI/SNF family chromatin remodeling protein. Although all CSB mutations that cause CS are recessive, the complete absence of CSB protein does not cause CS. In addition, most CSB mutations are located beyond exon ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1000031
更新日期:2008-03-21 00:00:00
abstract::Most B cell lymphomas originate from B cells that have germinal center (GC) experience and bear chromosome translocations and numerous point mutations. GC B cells remodel their immunoglobulin (Ig) genes by somatic hypermutation (SHM) and class switch recombination (CSR) in their Ig genes. Activation Induced Deaminase ...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008960
更新日期:2020-12-23 00:00:00
abstract::Histone modifiers are critical regulators of chromatin-based processes in eukaryotes. The histone methyltransferase Set1, a component of the Set1C/COMPASS complex, catalyzes the methylation at lysine 4 of histone H3 (H3K4me), a hallmark of euchromatin. Here, we show that the fission yeast Schizosaccharomyces pombe Set...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004740
更新日期:2014-10-30 00:00:00
abstract::This work analyses the genetic variation and evolutionary patterns of recessive resistance loci involved in matching-allele (MA) host-pathogen interactions, focusing on the pvr2 resistance gene to potyviruses of the wild pepper Capsicum annuum glabriusculum (chiltepin). Chiltepin grows in a variety of wild habitats in...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006214
更新日期:2016-08-04 00:00:00
abstract::Cooperative transcription factor binding at cis-regulatory sites in the genome drives robust eukaryotic gene expression, and many such sites must be coordinated to produce coherent transcriptional programs. The transcriptional program leading to motile cilia formation requires members of the DNA-binding forkhead (Fox)...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1006538
更新日期:2017-01-19 00:00:00
abstract::Centromere sequences are not conserved between species, and there is compelling evidence for epigenetic regulation of centromere identity, with location being dictated by the presence of chromatin containing the histone H3 variant CENP-A. Paradoxically, in most organisms CENP-A chromatin generally occurs on particular...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1004986
更新日期:2015-03-04 00:00:00
abstract::Efficient adaptation to iron starvation is an essential virulence determinant of the most common human mold pathogen, Aspergillus fumigatus. Here, we demonstrate that the cytosolic monothiol glutaredoxin GrxD plays an essential role in iron sensing in this fungus. Our studies revealed that (i) GrxD is essential for gr...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008379
更新日期:2019-09-16 00:00:00
abstract::Craniofacial abnormalities, including facial skeletal defects, comprise approximately one-third of all birth defects in humans. Since most bones in the face derive from cranial neural crest cells (CNCCs), which are multipotent stem cells, craniofacial bone disorders are largely attributed to defects in CNCCs. However,...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1007340
更新日期:2018-05-02 00:00:00
abstract::Sodalis glossinidius, a maternally inherited endosymbiont of the tsetse fly, maintains genes encoding homologues of the PhoP-PhoQ two-component regulatory system. This two-component system has been extensively studied in facultative bacterial pathogens and is known to serve as an environmental magnesium sensor and a r...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1002349
更新日期:2011-11-01 00:00:00
abstract::High glucose diets are unhealthy, although the mechanisms by which elevated glucose is harmful to whole animal physiology are not well understood. In Caenorhabditis elegans, high glucose shortens lifespan, while chemically inflicted glucose restriction promotes longevity. We investigated the impact of glucose metaboli...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1008982
更新日期:2020-08-25 00:00:00
abstract::The mechanism of circadian oscillations in mammals is cell autonomous and is generated by a set of genes that form a transcriptional autoregulatory feedback loop. While these "clock genes" are well conserved among animals, their specific functions remain to be fully understood and their roles in central versus periphe...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.0030033
更新日期:2007-02-23 00:00:00
abstract::The lineage factor Foxp3 is essential for the development and maintenance of regulatory T cells, but little is known about the mechanisms involved. Here, we demonstrate that an N-terminal proline-rich interaction region is crucial for Foxp3's function. Subdomains within this key region link Foxp3 to several independen...
journal_title:PLoS genetics
pub_type: 杂志文章
doi:10.1371/journal.pgen.1005251
更新日期:2015-06-24 00:00:00