Abstract:
BACKGROUND:Basement membrane (BM) is a thick sheet of extracellular matrix molecules, upon which epithelial cells attach. Various immunohistochemical studies in the past have been carried out but these advanced staining techniques are expensive and not feasible in routine laboratories. Although hematoxylin and eosin (H-E) is very popular among pathologists for looking at biopsies, the method has some limitations. This is where special stains come handy. AIMS AND OBJECTIVES:The aim of the present study was to demonstrate and compare the efficacy of H-E, periodic acid Schiff (PAS) and fluorescent periodic acid-acriflavine staining techniques for the basement membrane and to establish a histochemical stain which could be cost effective, less time consuming, and unambiguous for observation of the basement membrane zone. MATERIALS AND METHODS:A total number of 40 paraffin-embedded tissue sections of known basement membrane containing tissues including 10 - Normal oral mucosa (NOM) and 30 - oral lichen planus (OLP) were considered in the study. Four-micron-thick sections of each block were cut and stained with H-E stain, PAS and fluorescent periodic acid-acriflavine stain. Sections were evaluated by three oral pathologists independently for continuity, contrast and pattern. RESULTS:Though all the three stains showed favorable features at different levels, acriflavine stain was better than the other stains in demonstrating BM continuity, contrast and also the pattern followed by PAS stain. Acriflavine stain was the better in demonstrating a fibrillar pattern of a BM. Acriflavine stains a BM distinctly and is less time consuming and easy to carry out using readily available dyes as compared to other stains. CONCLUSION:The continuity and contrast along with the homogenous pattern and the afibrillar pattern of the BM was better demonstrated by acriflavine followed by the PAS stain.
journal_name
Indian J Dermatoljournal_title
Indian journal of dermatologyauthors
Pujar A,Pereira T,Tamgadge A,Bhalerao S,Tamgadge Sdoi
10.4103/0019-5154.159626subject
Has Abstractpub_date
2015-09-01 00:00:00pages
450-6issue
5eissn
0019-5154issn
1998-3611pii
IJD-60-450journal_volume
60pub_type
杂志文章abstract:AIM:To evaluate whether oxidative stress is implicated in melanocyte damage in vitiligo. BACKGROUND:Vitiligo is a complex disorder characterized by gradually enlarging areas of depigmentation. A new unifying hypothesis for the etiology of this pigment disorder is proposed, in which we postulate that the final destruct...
journal_title:Indian journal of dermatology
pub_type: 杂志文章,收录出版
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Indian journal of dermatology
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