Comparing The Efficacy of Hematoxylin and Eosin, Periodic Acid Schiff and Fluorescent Periodic Acid Schiff-Acriflavine Techniques for Demonstration of Basement Membrane in Oral Lichen Planus: A Histochemical Study.


BACKGROUND:Basement membrane (BM) is a thick sheet of extracellular matrix molecules, upon which epithelial cells attach. Various immunohistochemical studies in the past have been carried out but these advanced staining techniques are expensive and not feasible in routine laboratories. Although hematoxylin and eosin (H-E) is very popular among pathologists for looking at biopsies, the method has some limitations. This is where special stains come handy. AIMS AND OBJECTIVES:The aim of the present study was to demonstrate and compare the efficacy of H-E, periodic acid Schiff (PAS) and fluorescent periodic acid-acriflavine staining techniques for the basement membrane and to establish a histochemical stain which could be cost effective, less time consuming, and unambiguous for observation of the basement membrane zone. MATERIALS AND METHODS:A total number of 40 paraffin-embedded tissue sections of known basement membrane containing tissues including 10 - Normal oral mucosa (NOM) and 30 - oral lichen planus (OLP) were considered in the study. Four-micron-thick sections of each block were cut and stained with H-E stain, PAS and fluorescent periodic acid-acriflavine stain. Sections were evaluated by three oral pathologists independently for continuity, contrast and pattern. RESULTS:Though all the three stains showed favorable features at different levels, acriflavine stain was better than the other stains in demonstrating BM continuity, contrast and also the pattern followed by PAS stain. Acriflavine stain was the better in demonstrating a fibrillar pattern of a BM. Acriflavine stains a BM distinctly and is less time consuming and easy to carry out using readily available dyes as compared to other stains. CONCLUSION:The continuity and contrast along with the homogenous pattern and the afibrillar pattern of the BM was better demonstrated by acriflavine followed by the PAS stain.


Indian J Dermatol


Pujar A,Pereira T,Tamgadge A,Bhalerao S,Tamgadge S




Has Abstract


2015-09-01 00:00:00














  • Bullous lesions, sweat gland necrosis and rhabdomyolysis in alcoholic coma.

    abstract::A 42-year-old male developed hemorrhagic bullae and erosions while in alcohol induced coma. The lesions were limited to areas of the body in prolonged contact with the ground in the comatose state. He developed rhabdomyolysis, progressing to acute renal failure (ARF). Histopathological examination of the skin showed s...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Asokan N,Binesh VG,Andrews AM,Jayalakshmi PS

    更新日期:2014-11-01 00:00:00


    abstract::Patients on anti-TNFα therapy are at increased risk for rare opportunistic infections. Here we are reporting a case of Scedosporium apiospermum infection in a patient treated with anti-TNF for 5 years. Patients on anti-TNFα need close follow-up and clinicians should be suspicious for atypical infections in these immun...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Nguyen CT,Raychaudhuri SP

    更新日期:2011-01-01 00:00:00

  • Kindler'S syndrome: a case series of three Indian children.

    abstract::Kindler's syndrome is a very rare genodermatosis characterized by acral blistering starting in infancy, photosensitivity, progressive poikiloderma, cutaneous atrophy, and various forms of mucosal involvement. A large number of other cutaneous and extracutaneous features have also been described. We report here three c...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Ghosh SK,Bandyopadhyay D,Das J,Chatterjee G,Sarkar S

    更新日期:2010-10-01 00:00:00

  • Comparative assessment of the efficacy and safety of sertaconazole (2%) cream versus terbinafine cream (1%) versus luliconazole (1%) cream in patients with dermatophytoses: a pilot study.

    abstract:BACKGROUND:Sertaconazole is a new, broad spectrum, fungicidal and fungistatic imidazole with added antipruritic and anti-inflammatory activity that would provide greater symptomatic relief and hence would be beneficial in improving the quality of life for the patient with dermatophytoses. AIMS AND OBJECTIVES:To compar...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Jerajani H,Janaki C,Kumar S,Phiske M

    更新日期:2013-01-01 00:00:00

  • Clinical Profile of Androgenic Alopecia and Its Association with Cardiovascular Risk Factors.

    abstract:Context:Androgenic alopecia (AGA) is a hereditary androgen-dependent disorder, characterized by gradual conversion of terminal hair into miniaturized hair and defined by various patterns. Common age group affected is between 30 and 50 years. Metabolic syndrome (MetS) is a cluster of cardiovascular risk factors that inc...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Vora RV,Kota RKSK,Singhal RR,Anjaneyan G

    更新日期:2019-01-01 00:00:00

  • Evaluation of Efficacy, Safety, and Tolerability of Fixed Dose Combination (FDC) of Halometasone 0.05% and Fusidic Acid 2% W/W Topical Cream Versus FDC of Betamethasone Valerate 0.12% and Neomycin Sulphate 0.5% W/W Topical Cream in the Treatment of Infect

    abstract:AIM:To evaluate the efficacy and safety of fixed drug combination (FDC) halometasone 0.05% and fusidic acid 2% (group A) vs FDC betamethasone 0.12% and neomycin sulfate 0.5% cream (group B) in acute or chronic infected eczematous dermatosis, through a randomized open-label, comparative, multicentric study. MATERIALS A...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Pratap DV,Philip M,Rao NT,Jerajani HR,Kumar SA,Kuruvila M,Moodahadu LS,Dhawan S

    更新日期:2013-03-01 00:00:00

  • Oral manifestations of vitiligo.

    abstract:BACKGROUND:Vitiligo is one of the disorder that has social impact. Both skin and mucous membrane show depigmentation in vitiligo. Depigmentation in oral cavity can be more easily observed and the patient can be given awareness regarding the condition if they are unaware of vitiligo elsewhere in their body and can be gu...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Nagarajan A,Masthan MK,Sankar LS,Narayanasamy AB,Elumalai R

    更新日期:2015-01-01 00:00:00

  • Eccrine poroma on the face: an atypical presentation.

    abstract::An eccrine poroma is a solitary tumor arising from the eccrine duct epithelium in the epidermis. The lesions commonly occur on the sole of the foot, the hands, and occasionally on the nose, eyelids, neck, and chest. We report a patient who presented with a slow-growing nodular lesion over her left cheek, prompting a d...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Mahajan RS,Parikh AA,Chhajlani NP,Bilimoria FE

    更新日期:2014-01-01 00:00:00

  • Sensitization to Fungi in Atopic Dermatitis Patients 14 Year and Older - Association with Other Atopic Diseases and Parameters.

    abstract:Background:Fungi as a source of allergen are still largely neglected in basic research as well as in clinical practice. This study aimed to highlight the impact of fungal allergens in a group of patients suffering from atopic dermatitis (AD). Aims and Objectives:The evaluation of the sensitization to fungi in AD patie...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Celakovská J,Josef B,Ettler K,Vaneckova J,Ettlerova K,Jan K

    更新日期:2018-09-01 00:00:00

  • Giant pedunculated seborrheic keratosis of penis.

    abstract::Seborrheic keratosis of the penis is a rare entity. It has been mistaken as genital warts and differentiation is only made on histopathology. We are reporting a case presenting as multiple giant polypoidal lesions on the penile skin for the last 20 years. Seborrheic keratosis should be considered in the differential d...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Thakur JS,Thakur A,Chauhan CG,Diwana VK,Chauhan DC

    更新日期:2008-01-01 00:00:00

  • Lethal systemic degos disease with prominent cardio-pulmonary involvement.

    abstract::A 48-year-old man presented with acute abdominal pain underwent laparotomy that revealed two perforated ulcers in jejunum. He had skin lesions with porcelain white atrophic scar which were ignored for 3 years, whereas the disease revealed own malignant nature and progressed to nervous, gastrointestinal, and cardiopulm...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Ali YN,Hamed M,Azita N

    更新日期:2011-09-01 00:00:00

  • Comparative evaluation of long pulse Alexandrite laser and intense pulsed light systems for pseudofolliculitis barbae treatment with one year of follow up.

    abstract:BACKGROUND:Existing remedies for controlling pseudofolliculitis barbae (PFB) are sometimes helpful; however the positive effects are often short lived. The only definitive cure for PFB is permanent removal of the hair follicle. AIMS:Our aim was to compare the efficacy of the Alexandrite laser with the intense pulsed l...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Leheta TM

    更新日期:2009-01-01 00:00:00

  • Immune reconstitution inflammatory syndrome unmasking erythema nodosum leprosum: a rare case report.

    abstract::Immune reconstitution inflammatory syndrome (IRIS) occurs as an acute symptomatic expression of a latent infection during the recovery of immune system in response to antiretroviral therapy in HIV patients. IRIS triggers both opportunistic and non-opportunistic infections. We report a case of IRIS in a patient with HI...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Arakkal GK,Damarla SV,Chanda GM

    更新日期:2015-01-01 00:00:00

  • Decreased vitamin B12 and folic Acid concentrations in acne patients after isotretinoin therapy: a controlled study.

    abstract:BACKGROUND:Oral isotretinoin treatment might influence the levels of vitamin B12 and folic acid. AIMS AND OBJECTIVES:The aim of this study is to compare vitamin B12 and folic acid levels in patients with moderate and severe acne vulgaris with those of the healthy control group and to investigate the effect of isotreti...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Gökalp H,Bulur I,Gürer M

    更新日期:2014-11-01 00:00:00

  • Recurrent Pyogenic Granulomas with Underlying Arteriovenous Malformation: An Exclusively Rare Entity.

    abstract::We report a case of recurrent localized pyogenic granulomas with underlying arteriovenous malformation in an 11-year-old male child on upper back with onset in early infancy. ...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Majumdar B,Jain A,Bala S,Bandyopadhyay A,Singh A,Ghosh A

    更新日期:2015-07-01 00:00:00

  • Immunohistochemistry: relevance in dermatology.

    abstract::Use of immunohistochemical technique is increasing in diagnosing various diseases. In many situations it may not be possible to differentiate entities with overlapping clinical and histopathological features. Immunostaining of cellular antigens is immensely helpful in such cases. Immunohistochemistry (IHC) has also be...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Palit A,Inamadar AC

    更新日期:2011-11-01 00:00:00

  • Dermoscopy of Follicular Dowling-Degos Disease.

    abstract::Dowling-Degos disease (DDD) is a late-onset genodermatosis characterized by hyperpigmented macules on the flexures along with scattered comedo-like lesions and pitted acneiform scars. Follicular Dowling-Degos is a rare type of DDD, with only two reports so far. It presents with follicular papules and comedo-like lesio...

    journal_title:Indian journal of dermatology



    authors: Dabas G,Mahajan R,Afra TP,De D,Handa S,Aggarwal D,Radotra BD

    更新日期:2020-07-01 00:00:00

  • Hand, Foot and Mouth Disease in West Bengal, India: A Preliminary Report on Clinicovirological Trend over 3 Successive Years (2013-2015).

    abstract:INTRODUCTION:Hand, foot, and mouth disease (HFMD), an enteroviral disease has emerged as a major emerging infection in India. This is caused most commonly by enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) but can also be due to CVA4-10, CVA24, CVB2-5, and echovirus 18 (Echo18). Virological analysis of the cases o...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Sarma N,Chakraborty S,Dutta A,Sadhukhan PC

    更新日期:2017-09-01 00:00:00

  • Ichthyosis associated with rickets in two Indian children.

    abstract::We wish to report two cases of rickets due to vitamin D deficiency secondary to underlying ichthyotic skin disorder. The first case is of an 8-year-old male with history of multiple fluid-filled lesions over the body that would rupture to heal with thickening and scaling of skin, suggestive of epidermolytic hyperkerat...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Kothari D,Doshi B,Garg G,Khopkar US

    更新日期:2013-05-01 00:00:00

  • Apoptosis and clinical severity in patients with psoriasis and HCV infection.

    abstract:BACKGROUND:It has been proposed that hepatitis C virus (HCV) antigens are involved in the pathogenesis of psoriasis and may contribute to severity of the disease. Increased expression of the apoptosis-regulating proteins p53 and tTG and decreased levels of bcl-2 in the keratinocytes of the skin of psoriatic patients ha...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Gabr SA,Berika MY,Alghadir AH

    更新日期:2014-05-01 00:00:00

  • Acetaminophen-induced cellulitis-like fixed drug eruption.

    abstract::Acetaminophen is a widely used analgesic drug. Its adverse reactions are rare but severe. An 89-year-old man developed an indurated edematous and erythematous plaque on his left arm 1 day after acetaminophen ingestion. Cellulitis was suspected and antibiotictherapy was started but there was no improvement of the rash;...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Fathallah N,Ben Salem C,Slim R,Boussofara L,Ghariani N,Bouraoui K

    更新日期:2011-03-01 00:00:00

  • Generalized granuloma annulare treated with monthly rifampicin, ofloxacin, and minocycline combination therapy.

    abstract::Granuloma annulare (GA) is a disease characterized by granulomatous inflammation of the dermis. A variant form of the disease, generalized granuloma annulare (GGA), can be observed in 15% of affected patients. Localized GA is likely to resolve spontaneously within months or a few years, whereas GGA can persist for dec...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Garg S,Baveja S

    更新日期:2013-05-01 00:00:00

  • Acrocyanosis: an overview.

    abstract:INTRODUCTION:It is a functional peripheral vascular disorder characterized by bluish discoloration of skin and mucous membrane due to diminished oxyhemoglobin. It may be due to central or local tissue oxygenation defects. It is a painful episodic disorder, where trophic changes and ulceration are very rare except in ne...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Das S,Maiti A

    更新日期:2013-11-01 00:00:00

  • Use of Topical Rapamycin in Facial Angiofibromas in Indian Skin Type.

    abstract:INTRODUCTION:Facial angiofibromas (FA) are the most visible cutaneous manifestations in patients with tuberous sclerosis (TS), often resulting in stigmatization of the affected individuals. Recent studies have suggested that topical rapamycin may be an effective treatment for angiofibromas. AIM:To study the safety and...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Viswanath V,Thakur P,Pund P

    更新日期:2016-01-01 00:00:00

  • The Evaluation of Endothelin-1 and Endothelin Receptor Type A Gene Polymorphisms in Patients with Vitiligo.

    abstract:BACKGROUND:Endothelin-1 (EDN1) and EDN receptor type A (EDNRA) are implicated in melanocyte functions. AIM AND OBJECTIVES:This study examines the role of EDN1 (G5665T and T-1370G) and EDNRA (C + 70G and G-231A) polymorphisms as a risk factor for vitiligo, and evaluates the relationship between genotypes and clinical c...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Bingül İ,Aydıngöz İE,Vural P,Doğru-Abbasoğlu S,Uysal M

    更新日期:2016-01-01 00:00:00

  • No Evidence of Human Papillomaviruses in Non-genital Seborrheic Keratosis.

    abstract:BACKGROUND:Seborrheic keratosis (SK) is a benign epidermal tumor of unknown etiology. Because of its wart-like morphology, Human papillomaviruses (HPVs) have been suggested as a possible causative agent. Viral involvement, however, has not been confirmed yet despite research and the association between HPVs and seborrh...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Meibodi NT,Nahidi Y,Meshkat Z,Esmaili H,Gharib M,Gholoobi A

    更新日期:2013-07-01 00:00:00

  • Immunotherapy with intralesional Candida albicans antigen in resistant or recurrent warts: a study.

    abstract:BACKGROUND:Warts are sometimes resistant or they tend to recur after every possible destructive therapy. Immunotherapy with skin-test antigens has been used as a viable therapeutic option in such recalcitrant cases. AIM:The aim of the study was to evaluate the response of resistant or recurrent warts to intralesional ...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Majid I,Imran S

    更新日期:2013-09-01 00:00:00

  • Topical Corticosteroids Abuse: A Clinical Study of Cutaneous Adverse Effects.

    abstract:Background:Topical corticosteroids (TC) are one of the most widely used agents in dermatology practice. Misuse of these agents may lead to a wide range of adverse effects. Aim:This study was conducted to assess the magnitude of abuse of topical corticosteroids (TC) and clinical patterns of cutaneous adverse effects am...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Meena S,Gupta LK,Khare AK,Balai M,Mittal A,Mehta S,Bhatri G

    更新日期:2017-11-01 00:00:00

  • POEMS Syndrome with Extensive Calciphylaxis: A Case Report and Literature Review.

    abstract::POEMS syndrome, a paraneoplastic disorder, caused by plasma cell dyscrasia, is characterized by polyneuropathy, organomegaly, endocrinopathy, M-protein, and skin changes. Calciphylaxis is a vasculopathy of subcutaneous arteries with calcification and skin ulceration, most commonly associated with end-stage renal disea...

    journal_title:Indian journal of dermatology



    authors: Liu CH,Chen YC,Chen TY,Huang HW,Lee JY

    更新日期:2020-11-01 00:00:00

  • Foot eczema: the role of patch test in determining the causative agent using standard series.

    abstract::Foot dermatitis refers to the predominant involvement of feet in the eczematous process. This study is undertaken to determine the clinical pattern and causative agent in foot eczema and to evaluate the role of patch testing in determining the causative agent of foot eczema. Data was collected from 50 patients with fo...

    journal_title:Indian journal of dermatology

    pub_type: 杂志文章


    authors: Priya KS,Kamath G,Martis J,D S,Shetty NJ,Bhat RM,Kishore BN

    更新日期:2008-01-01 00:00:00