Differential regulation of receptivity in two uterine horns of a recipient mouse following asynchronous embryo transfer.

Abstract:

:Receptivity is a limited time in which uterine endometrium can establish a successful dialogue with blastocyst. This study was to investigate the effect of asynchronous embryo transfer on uterine receptivity in mice. Embryos under different stages were transferred into two oviduct sides of a recipient mouse on day 1 of pseudopregnancy. Our results showed the asynchronously transferred embryos can implant in all groups. Compared to zygote-transfer group, the length of implanted embryos is longer in 8-cell embryo- or blastocyst-transfer group. The levels of Snail and COX-2 immunostaining in blastocyst-transfer group are significantly stronger than that in zygote-transfer group. Embryos in blastocyst-transfer group migrate faster than that in zygote-transfer group within uterus. Blastocysts are in a state of developmental delay after they are transferred into oviducts, and they are reactivated and implanted rapidly in uterus. The developmental rate to newborn in zygote-transfer group is obviously higher than that in blastocyst-transfer group, suggesting that a delay in embryo development and implantation will lead to a decrease of litter size. These results indicated that the window of implantation is differentially regulated in two uterine horns of a recipient by embryos at different stages.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Li SJ,Wang TS,Qin FN,Huang Z,Liang XH,Gao F,Song Z,Yang ZM

doi

10.1038/srep15897

subject

Has Abstract

pub_date

2015-11-04 00:00:00

pages

15897

issn

2045-2322

pii

srep15897

journal_volume

5

pub_type

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