Exocyst-Dependent Membrane Addition Is Required for Anaphase Cell Elongation and Cytokinesis in Drosophila.

Abstract:

:Mitotic and cytokinetic processes harness cell machinery to drive chromosomal segregation and the physical separation of dividing cells. Here, we investigate the functional requirements for exocyst complex function during cell division in vivo, and demonstrate a common mechanism that directs anaphase cell elongation and cleavage furrow progression during cell division. We show that onion rings (onr) and funnel cakes (fun) encode the Drosophila homologs of the Exo84 and Sec8 exocyst subunits, respectively. In onr and fun mutant cells, contractile ring proteins are recruited to the equatorial region of dividing spermatocytes. However, cytokinesis is disrupted early in furrow ingression, leading to cytokinesis failure. We use high temporal and spatial resolution confocal imaging with automated computational analysis to quantitatively compare wild-type versus onr and fun mutant cells. These results demonstrate that anaphase cell elongation is grossly disrupted in cells that are compromised in exocyst complex function. Additionally, we observe that the increase in cell surface area in wild type peaks a few minutes into cytokinesis, and that onr and fun mutant cells have a greatly reduced rate of surface area growth specifically during cell division. Analysis by transmission electron microscopy reveals a massive build-up of cytoplasmic astral membrane and loss of normal Golgi architecture in onr and fun spermatocytes, suggesting that exocyst complex is required for proper vesicular trafficking through these compartments. Moreover, recruitment of the small GTPase Rab11 and the PITP Giotto to the cleavage site depends on wild-type function of the exocyst subunits Exo84 and Sec8. Finally, we show that the exocyst subunit Sec5 coimmunoprecipitates with Rab11. Our results are consistent with the exocyst complex mediating an essential, coordinated increase in cell surface area that potentiates anaphase cell elongation and cleavage furrow ingression.

journal_name

PLoS Genet

journal_title

PLoS genetics

authors

Giansanti MG,Vanderleest TE,Jewett CE,Sechi S,Frappaolo A,Fabian L,Robinett CC,Brill JA,Loerke D,Fuller MT,Blankenship JT

doi

10.1371/journal.pgen.1005632

subject

Has Abstract

pub_date

2015-11-03 00:00:00

pages

e1005632

issue

11

eissn

1553-7390

issn

1553-7404

pii

PGENETICS-D-15-01267

journal_volume

11

pub_type

杂志文章
  • BOD1 Is Required for Cognitive Function in Humans and Drosophila.

    abstract::Here we report a stop-mutation in the BOD1 (Biorientation Defective 1) gene, which co-segregates with intellectual disability in a large consanguineous family, where individuals that are homozygous for the mutation have no detectable BOD1 mRNA or protein. The BOD1 protein is required for proper chromosome segregation,...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1006022

    authors: Esmaeeli-Nieh S,Fenckova M,Porter IM,Motazacker MM,Nijhof B,Castells-Nobau A,Asztalos Z,Weißmann R,Behjati F,Tzschach A,Felbor U,Scherthan H,Sayfati SM,Ropers HH,Kahrizi K,Najmabadi H,Swedlow JR,Schenck A,Kuss AW

    更新日期:2016-05-11 00:00:00

  • Ribosome synthesis and MAPK activity modulate ionizing radiation-induced germ cell apoptosis in Caenorhabditis elegans.

    abstract::Synthesis of ribosomal RNA by RNA polymerase I (RNA pol I) is an elemental biological process and is key for cellular homeostasis. In a forward genetic screen in C. elegans designed to identify DNA damage-response factors, we isolated a point mutation of RNA pol I, rpoa-2(op259), that leads to altered rRNA synthesis a...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1003943

    authors: Eberhard R,Stergiou L,Hofmann ER,Hofmann J,Haenni S,Teo Y,Furger A,Hengartner MO

    更新日期:2013-11-01 00:00:00

  • Polymorphisms in the yeast galactose sensor underlie a natural continuum of nutrient-decision phenotypes.

    abstract::In nature, microbes often need to "decide" which of several available nutrients to utilize, a choice that depends on a cell's inherent preference and external nutrient levels. While natural environments can have mixtures of different nutrients, phenotypic variation in microbes' decisions of which nutrient to utilize i...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1006766

    authors: Lee KB,Wang J,Palme J,Escalante-Chong R,Hua B,Springer M

    更新日期:2017-05-24 00:00:00

  • Correction: Functional male accessory glands and fertility in Drosophila require novel ecdysone receptor.

    abstract::[This corrects the article DOI: 10.1371/journal.pgen.1006788.]. ...

    journal_title:PLoS genetics

    pub_type: 已发布勘误

    doi:10.1371/journal.pgen.1006893

    authors: Sharma V,Pandey AK,Kumar A,Misra S,Gupta HPK,Gupta S,Singh A,Buehner NA,Ravi Ram K

    更新日期:2017-07-12 00:00:00

  • Limiting of the innate immune response by SF3A-dependent control of MyD88 alternative mRNA splicing.

    abstract::Controlling infectious disease without inducing unwanted inflammatory disease requires proper regulation of the innate immune response. Thus, innate immunity needs to be activated when needed during an infection, but must be limited to prevent damage. To accomplish this, negative regulators of innate immunity limit th...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1003855

    authors: De Arras L,Alper S

    更新日期:2013-10-01 00:00:00

  • Genetic tests for ecological and allopatric speciation in anoles on an island archipelago.

    abstract::From Darwin's study of the Galapagos and Wallace's study of Indonesia, islands have played an important role in evolutionary investigations, and radiations within archipelagos are readily interpreted as supporting the conventional view of allopatric speciation. Even during the ongoing paradigm shift towards other mode...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000929

    authors: Thorpe RS,Surget-Groba Y,Johansson H

    更新日期:2010-04-29 00:00:00

  • The Zic family homologue Odd-paired regulates Alk expression in Drosophila.

    abstract::The Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) plays a critical role in the specification of founder cells (FCs) in the Drosophila visceral mesoderm (VM) during embryogenesis. Reporter gene and CRISPR/Cas9 deletion analysis reveals enhancer regions in and upstream of the Alk locus that influence t...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1006617

    authors: Mendoza-García P,Hugosson F,Fallah M,Higgins ML,Iwasaki Y,Pfeifer K,Wolfstetter G,Varshney G,Popichenko D,Gergen JP,Hens K,Deplancke B,Palmer RH

    更新日期:2017-04-03 00:00:00

  • Recovery from an acute infection in C. elegans requires the GATA transcription factor ELT-2.

    abstract::The mechanisms involved in the recognition of microbial pathogens and activation of the immune system have been extensively studied. However, the mechanisms involved in the recovery phase of an infection are incompletely characterized at both the cellular and physiological levels. Here, we establish a Caenorhabditis e...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1004609

    authors: Head B,Aballay A

    更新日期:2014-10-23 00:00:00

  • A genome-wide association study of pulmonary function measures in the Framingham Heart Study.

    abstract::The ratio of forced expiratory volume in one second to forced vital capacity (FEV(1)/FVC) is a measure used to diagnose airflow obstruction and is highly heritable. We performed a genome-wide association study in 7,691 Framingham Heart Study participants to identify single-nucleotide polymorphisms (SNPs) associated wi...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000429

    authors: Wilk JB,Chen TH,Gottlieb DJ,Walter RE,Nagle MW,Brandler BJ,Myers RH,Borecki IB,Silverman EK,Weiss ST,O'Connor GT

    更新日期:2009-03-01 00:00:00

  • Ataxin1L is a regulator of HSC function highlighting the utility of cross-tissue comparisons for gene discovery.

    abstract::Hematopoietic stem cells (HSCs) are rare quiescent cells that continuously replenish the cellular components of the peripheral blood. Observing that the ataxia-associated gene Ataxin-1-like (Atxn1L) was highly expressed in HSCs, we examined its role in HSC function through in vitro and in vivo assays. Mice lacking Atx...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1003359

    authors: Kahle JJ,Souroullas GP,Yu P,Zohren F,Lee Y,Shaw CA,Zoghbi HY,Goodell MA

    更新日期:2013-03-01 00:00:00

  • Chromosome 9p21 SNPs Associated with Multiple Disease Phenotypes Correlate with ANRIL Expression.

    abstract::Single nucleotide polymorphisms (SNPs) on chromosome 9p21 are associated with coronary artery disease, diabetes, and multiple cancers. Risk SNPs are mainly non-coding, suggesting that they influence expression and may act in cis. We examined the association between 56 SNPs in this region and peripheral blood expressio...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000899

    authors: Cunnington MS,Santibanez Koref M,Mayosi BM,Burn J,Keavney B

    更新日期:2010-04-08 00:00:00

  • Combinations of DIPs and Dprs control organization of olfactory receptor neuron terminals in Drosophila.

    abstract::In Drosophila, 50 classes of olfactory receptor neurons (ORNs) connect to 50 class-specific and uniquely positioned glomeruli in the antennal lobe. Despite the identification of cell surface receptors regulating axon guidance, how ORN axons sort to form 50 stereotypical glomeruli remains unclear. Here we show that the...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1007560

    authors: Barish S,Nuss S,Strunilin I,Bao S,Mukherjee S,Jones CD,Volkan PC

    更新日期:2018-08-13 00:00:00

  • The C-terminal of CASY-1/Calsyntenin regulates GABAergic synaptic transmission at the Caenorhabditis elegans neuromuscular junction.

    abstract::The C. elegans ortholog of mammalian calsyntenins, CASY-1, is an evolutionarily conserved type-I transmembrane protein that is highly enriched in the nervous system. Mammalian calsyntenins are strongly expressed at inhibitory synapses, but their role in synapse development and function is still elusive. Here, we repor...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1007263

    authors: Thapliyal S,Vasudevan A,Dong Y,Bai J,Koushika SP,Babu K

    更新日期:2018-03-12 00:00:00

  • Unique genetic signatures of local adaptation over space and time for diapause, an ecologically relevant complex trait, in Drosophila melanogaster.

    abstract::Organisms living in seasonally variable environments utilize cues such as light and temperature to induce plastic responses, enabling them to exploit favorable seasons and avoid unfavorable ones. Local adapation can result in variation in seasonal responses, but the genetic basis and evolutionary history of this varia...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1009110

    authors: Erickson PA,Weller CA,Song DY,Bangerter AS,Schmidt P,Bergland AO

    更新日期:2020-11-20 00:00:00

  • The (p)ppGpp-binding GTPase Era promotes rRNA processing and cold adaptation in Staphylococcus aureus.

    abstract::Ribosome assembly cofactors are widely conserved across all domains of life. One such group, the ribosome-associated GTPases (RA-GTPase), act as molecular switches to coordinate ribosome assembly. We previously identified the Staphylococcus aureus RA-GTPase Era as a target for the stringent response alarmone (p)ppGpp,...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1008346

    authors: Wood A,Irving SE,Bennison DJ,Corrigan RM

    更新日期:2019-08-29 00:00:00

  • PNUTS/PP1 regulates RNAPII-mediated gene expression and is necessary for developmental growth.

    abstract::In multicellular organisms, tight regulation of gene expression ensures appropriate tissue and organismal growth throughout development. Reversible phosphorylation of the RNA Polymerase II (RNAPII) C-terminal domain (CTD) is critical for the regulation of gene expression states, but how phosphorylation is actively mod...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1003885

    authors: Ciurciu A,Duncalf L,Jonchere V,Lansdale N,Vasieva O,Glenday P,Rudenko A,Vissi E,Cobbe N,Alphey L,Bennett D

    更新日期:2013-10-01 00:00:00

  • A genome-wide characterization of microRNA genes in maize.

    abstract::MicroRNAs (miRNAs) are small, non-coding RNAs that play essential roles in plant growth, development, and stress response. We conducted a genome-wide survey of maize miRNA genes, characterizing their structure, expression, and evolution. Computational approaches based on homology and secondary structure modeling ident...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000716

    authors: Zhang L,Chia JM,Kumari S,Stein JC,Liu Z,Narechania A,Maher CA,Guill K,McMullen MD,Ware D

    更新日期:2009-11-01 00:00:00

  • Windei, the Drosophila homolog of mAM/MCAF1, is an essential cofactor of the H3K9 methyl transferase dSETDB1/Eggless in germ line development.

    abstract::The epigenetic regulation of gene expression by the covalent modification of histones is a fundamental mechanism required for the proper differentiation of germ line cells during development. Trimethylation of histone 3 lysine 9 (H3K9me3) leads to chromatin silencing and the formation of heterochromatin by recruitment...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000644

    authors: Koch CM,Honemann-Capito M,Egger-Adam D,Wodarz A

    更新日期:2009-09-01 00:00:00

  • Biased, non-equivalent gene-proximal and -distal binding motifs of orphan nuclear receptor TR4 in primary human erythroid cells.

    abstract::We previously reported that TR2 and TR4 orphan nuclear receptors bind to direct repeat (DR) elements in the ε- and γ-globin promoters, and act as molecular anchors for the recruitment of epigenetic corepressors of the multifaceted DRED complex, thereby leading to ε- and γ-globin transcriptional repression during defin...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1004339

    authors: Shi L,Sierant MC,Gurdziel K,Zhu F,Cui S,Kolodziej KE,Strouboulis J,Guan Y,Tanabe O,Lim KC,Engel JD

    更新日期:2014-05-08 00:00:00

  • SU(VAR)3-7 links heterochromatin and dosage compensation in Drosophila.

    abstract::In Drosophila, dosage compensation augments X chromosome-linked transcription in males relative to females. This process is achieved by the Dosage Compensation Complex (DCC), which associates specifically with the male X chromosome. We previously found that the morphology of this chromosome is sensitive to the amounts...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000066

    authors: Spierer A,Begeot F,Spierer P,Delattre M

    更新日期:2008-05-02 00:00:00

  • Sex reversal in zebrafish fancl mutants is caused by Tp53-mediated germ cell apoptosis.

    abstract::The molecular genetic mechanisms of sex determination are not known for most vertebrates, including zebrafish. We identified a mutation in the zebrafish fancl gene that causes homozygous mutants to develop as fertile males due to female-to-male sex reversal. Fancl is a member of the Fanconi Anemia/BRCA DNA repair path...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1001034

    authors: Rodríguez-Marí A,Cañestro C,Bremiller RA,Nguyen-Johnson A,Asakawa K,Kawakami K,Postlethwait JH

    更新日期:2010-07-22 00:00:00

  • Common genetic variants and modification of penetrance of BRCA2-associated breast cancer.

    abstract::The considerable uncertainty regarding cancer risks associated with inherited mutations of BRCA2 is due to unknown factors. To investigate whether common genetic variants modify penetrance for BRCA2 mutation carriers, we undertook a two-staged genome-wide association study in BRCA2 mutation carriers. In stage 1 using ...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1001183

    authors: Gaudet MM,Kirchhoff T,Green T,Vijai J,Korn JM,Guiducci C,Segrè AV,McGee K,McGuffog L,Kartsonaki C,Morrison J,Healey S,Sinilnikova OM,Stoppa-Lyonnet D,Mazoyer S,Gauthier-Villars M,Sobol H,Longy M,Frenay M,GEMO Study

    更新日期:2010-10-28 00:00:00

  • Phoenix is required for mechanosensory hair cell regeneration in the zebrafish lateral line.

    abstract::In humans, the absence or irreversible loss of hair cells, the sensory mechanoreceptors in the cochlea, accounts for a large majority of acquired and congenital hearing disorders. In the auditory and vestibular neuroepithelia of the inner ear, hair cells are accompanied by another cell type called supporting cells. Th...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000455

    authors: Behra M,Bradsher J,Sougrat R,Gallardo V,Allende ML,Burgess SM

    更新日期:2009-04-01 00:00:00

  • Genome sequence of the plant growth promoting endophytic bacterium Enterobacter sp. 638.

    abstract::Enterobacter sp. 638 is an endophytic plant growth promoting gamma-proteobacterium that was isolated from the stem of poplar (Populus trichocarpaxdeltoides cv. H11-11), a potentially important biofuel feed stock plant. The Enterobacter sp. 638 genome sequence reveals the presence of a 4,518,712 bp chromosome and a 157...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1000943

    authors: Taghavi S,van der Lelie D,Hoffman A,Zhang YB,Walla MD,Vangronsveld J,Newman L,Monchy S

    更新日期:2010-05-13 00:00:00

  • Deep sequencing of plant and animal DNA contained within traditional Chinese medicines reveals legality issues and health safety concerns.

    abstract::Traditional Chinese medicine (TCM) has been practiced for thousands of years, but only within the last few decades has its use become more widespread outside of Asia. Concerns continue to be raised about the efficacy, legality, and safety of many popular complementary alternative medicines, including TCMs. Ingredients...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1002657

    authors: Coghlan ML,Haile J,Houston J,Murray DC,White NE,Moolhuijzen P,Bellgard MI,Bunce M

    更新日期:2012-01-01 00:00:00

  • Positive selection for new disease mutations in the human germline: evidence from the heritable cancer syndrome multiple endocrine neoplasia type 2B.

    abstract::Multiple endocrine neoplasia type 2B (MEN2B) is a highly aggressive thyroid cancer syndrome. Since almost all sporadic cases are caused by the same nucleotide substitution in the RET proto-oncogene, the calculated disease incidence is 100-200 times greater than would be expected based on the genome average mutation fr...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1002420

    authors: Choi SK,Yoon SR,Calabrese P,Arnheim N

    更新日期:2012-01-01 00:00:00

  • Genetic interactions due to constitutive and inducible gene regulation mediated by the unfolded protein response in C. elegans.

    abstract::The unfolded protein response (UPR) is an adaptive signaling pathway utilized to sense and alleviate the stress of protein folding in the endoplasmic reticulum (ER). In mammals, the UPR is mediated through three proximal sensors PERK/PEK, IRE1, and ATF6. PERK/PEK is a protein kinase that phosphorylates the alpha subun...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.0010037

    authors: Shen X,Ellis RE,Sakaki K,Kaufman RJ

    更新日期:2005-09-01 00:00:00

  • The topoisomerase 3α zinc-finger domain T1 of Arabidopsis thaliana is required for targeting the enzyme activity to Holliday junction-like DNA repair intermediates.

    abstract::Topoisomerase 3α, a class I topoisomerase, consists of a TOPRIM domain, an active centre and a variable number of zinc-finger domains (ZFDs) at the C-terminus, in multicellular organisms. Whereas the functions of the TOPRIM domain and the active centre are known, the specific role of the ZFDs is still obscure. In cont...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1007674

    authors: Dorn A,Röhrig S,Papp K,Schröpfer S,Hartung F,Knoll A,Puchta H

    更新日期:2018-09-17 00:00:00

  • The C. elegans Discoidin Domain Receptor DDR-2 Modulates the Met-like RTK-JNK Signaling Pathway in Axon Regeneration.

    abstract::The ability of specific neurons to regenerate their axons after injury is governed by cell-intrinsic regeneration pathways. However, the signaling pathways that orchestrate axon regeneration are not well understood. In Caenorhabditis elegans, initiation of axon regeneration is positively regulated by SVH-2 Met-like gr...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1006475

    authors: Hisamoto N,Nagamori Y,Shimizu T,Pastuhov SI,Matsumoto K

    更新日期:2016-12-16 00:00:00

  • Recurrent loss of specific introns during angiosperm evolution.

    abstract::Numerous instances of presence/absence variations for introns have been documented in eukaryotes, and some cases of recurrent loss of the same intron have been suggested. However, there has been no comprehensive or phylogenetically deep analysis of recurrent intron loss. Of 883 cases of intron presence/absence variati...

    journal_title:PLoS genetics

    pub_type: 杂志文章

    doi:10.1371/journal.pgen.1004843

    authors: Wang H,Devos KM,Bennetzen JL

    更新日期:2014-12-04 00:00:00