Cloning, mRNA expression and transcriptional regulation of five retinoid X receptor subtypes in yellow catfish Pelteobagrus fulvidraco by insulin.


:Retinoid X receptors (RXRs) are members of the nuclear receptor superfamily and mediate development, reproduction, homeostasis and cell differentiation processes in vertebrates. In this study, full-length cDNA sequences of five rxr subtypes from yellow catfish Pelteobagrus fulvidraco were cloned. Their mRNA expression patterns in different tissues and transcriptional regulation by insulin were determined. Five P. fulvidraco rxr (Pf-rxr) subtypes differed in the length of cDNA sequence and the open reading frame, but shared the similar domain structures as in typical nuclear receptors. Phylogenetic analysis revealed that the five Pf-rxr subtypes were paralogous genes, and that Pf-rxrβa and Pf-rxrβb had arisen during a teleost-specific genome duplication event. Five subtypes of Pf-rxr were detected in all the tested tissues. Overlapping and distinct expression patterns were found for different Pf-rxr subtypes, suggesting functional redundancy and divergence of these duplicates. Intraperitoneal insulin injection and incubation reduced the mRNA expression of Pf-rxrgb, but not other subtypes, in the liver and hepatocytes of P. fulvidraco, respectively, suggesting that Pf-rxrgb is the dominant rxr subtype involved in the insulin signaling pathway in P. fulvidraco.


Gen Comp Endocrinol


Pan YX,Luo Z,Wu K,Zhang LH,Xu YH,Chen QL




Has Abstract


2016-01-01 00:00:00












  • Methyl farnesoate stimulates ecdysteroid secretion from crab Y-organs in vitro.

    abstract::Methyl farnesoate (MF) stimulation of ecdysteroid secretion by Y-organs of the crab, Cancer magister, is demonstrated. Isolated Y-organs that were incubated with a mandibular organ (MO) secreted significantly higher levels of ecdysteroids into the medium when compared to those incubated in the absence of a MO. MOs sec...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Tamone SL,Chang ES

    更新日期:1993-03-01 00:00:00

  • Circadian rhythms of thyroid secretion, morphometry, and cell division in prometamorphic and climax Rana tadpoles.

    abstract::Rhythmicity of thyroid follicle structure, cell division, and T4 secretion in vitro was studied in anuran tadpoles on a 12L:12D cycle with light onset at 0800 hr. Stage XIII Rana pipiens larvae had a significant circadian rhythm of follicle lumen diameter (acrophase 1052 hr). Follicle cell height was higher at 0900 hr...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Wright ML,Blanchard LS,Pikula A,Labieniec KE

    更新日期:1995-07-01 00:00:00

  • Molecular characterization of insulin-like androgenic gland hormone-binding protein gene from the oriental river prawn Macrobrachium nipponense and investigation of its transcriptional relationship with the insulin-like androgenic gland hormone gene.

    abstract::Insulin-like androgenic gland hormone-binding protein (IAGBP) has been investigated in crustaceans in vitro. However, the relationship between IAGBP and its putative binding protein partner insulin-like androgenic gland hormone (IAG) has not been studied at the transcriptional level in vivo. In the current study, we c...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Li F,Bai H,Xiong Y,Fu H,Jiang S,Jiang F,Jin S,Sun S,Qiao H,Zhang W

    更新日期:2015-05-15 00:00:00

  • Clarification of emu serum for peptide hormone assay using polyethylene glycol precipitation.

    abstract::Interference in radioimmunoassays (RIAs) was frequently encountered during endocrinological studies of the emu (Dromaius novaehollandiae). Interference was greatest when serum was cloudy or opaque. Such samples appeared seasonally, in spring and summer during the phase of fat deposition, and in the winter when females...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Van Cleeff JK,Blackberry MA,Blache D,Martin GB

    更新日期:2003-06-15 00:00:00

  • Influence of potassium thiocyanate on thyroid function of rainbow trout, Salmo gairdneri.

    abstract::Sublethal doses of potassium thiocyanate (KSCN), an iodide competitor, depressed thyroidal radioiodide uptake of fed laboratory rainbow trout at 11 degrees, but did not depress either plasma levels of T4 (L-thyroxine) and T3 (triiodo-L-thyronine) or radioiodide incorporation into plasma iodothyronines. Furthermore, pl...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Eales JG,Shostak S

    更新日期:1983-07-01 00:00:00

  • Characterization of stress response involved in chicken myopathy.

    abstract::Myopathies (Woody Breast (WB) and White Striping (WS)) of broiler chickens have been correlated with fast growth. Recent studies reported that localized hypoxia and metabolic impairment may involve in these myopathies of birds. In order to better understand the stress response mechanisms affecting myopathies of broile...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Kang SW,Kidd MT Jr,Kadhim HJ,Shouse S,Orlowski SK,Hiltz J,Anthony NB,Kuenzel WJ,Kong BC

    更新日期:2020-09-01 00:00:00

  • Gene expression profiling during spermatogenesis in early maturing male Atlantic salmon parr testes.

    abstract::The initiation of sexual maturation and spermatogenesis are complex processes that require the highly coordinated regulation of a number of key genes. The endocrine system plays crucial roles in these processes, but the precise mechanisms involved in sexual maturation of fish are poorly understood. We investigated the...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Maugars G,Schmitz M

    更新日期:2008-11-01 00:00:00

  • Ecdysteroid levels in Daphnia magna during a molt cycle: determination by radioimmunoassay (RIA) and liquid chromatography-mass spectrometry (LC-MS).

    abstract::Despite their abundance and their enormous significance for various ecological processes, endocrine systems of microcrustaceans have been poorly investigated. Here, we used a highly sensitive radioimmunoassay (RIA) to determine free and conjugated ecdysteroid levels in whole-body extracts of adult Daphnia magna during...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Martin-Creuzburg D,Westerlund SA,Hoffmann KH

    更新日期:2007-03-01 00:00:00

  • Androgen and estrogen levels in the plasma of Pleurodeles waltl, Michah., during the annual cycle. II. Female cycle.

    abstract::Sex steroid levels in the plasma of the female Pleurodeles waltl were determined by radioimmunoassay of blood samples taken monthly from each newt during an 18-month period. Estradiol-17 beta levels were relatively high (greater than 1 ng/ml) but androgen levels were higher (2-30 ng/ml). Androgen and estradiol-17 beta...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Garnier DH

    更新日期:1985-12-01 00:00:00

  • Environmental influences on Adelie penguin breeding schedules, endocrinology, and chick survival.

    abstract::To understand how the social and physical environment influences behaviour, reproduction and survival, studies of underlying hormonal processes are crucial; in particular, interactions between stress and reproductive responses may have critical influences on breeding schedules. Several authors have examined the timing...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Ninnes CE,Waas JR,Ling N,Nakagawa S,Banks JC,Bell DG,Bright A,Carey PW,Chandler J,Hudson QJ,Ingram JR,Lyall K,Morgan DK,Stevens MI,Wallace J,Möstl E

    更新日期:2011-08-01 00:00:00

  • Locust diuretic hormone-stimulated synthesis and excretion of cyclic-AMP: a novel Malpighian tubule bioassay.

    abstract::Endogenous cyclic-AMP concentrations in Malpighian tubules of locusts increase as a result of diuretic hormone stimulation, and the level of increase is shown to be dependent on basal intracellular levels of cyclic-AMP. Furthermore, the increase in endogenous cyclic-AMP is in such excess as to cause excretion of high ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Rafaeli A,Pines M,Stern PS,Applebaum SW

    更新日期:1984-04-01 00:00:00

  • Sexual dimorphism in numbers of vasotocin-immunoreactive neurons in brain areas associated with reproductive behaviors in the roughskin newt.

    abstract::Vasotocin (VT) and vasopressin control many endocrine and neuroendocrine functions, including the regulation of reproductive behaviors. In the roughskin newt (Taricha granulosa), VT administration can enhance courtship behaviors in males and egg-laying behaviors in females. This study used immunohistochemistry to inve...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Moore FL,Richardson C,Lowry CA

    更新日期:2000-02-01 00:00:00

  • In vitro study of the direct ovarian effects of gonadotropin-releasing hormone (GnRH) in the frogs, Rana pipiens and Rana catesbeiana.

    abstract::Ovaries from the leopard frog, Rana pipiens, and bullfrog, R. catesbeiana, were used to study potential direct extrapituitary effects of gonadotropin-releasing hormone (GnRH). GnRH alone did not alter steroid secretion from ovaries in either species. Ovarian fragments containing mature follicles from R. pipiens were i...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Hubbard GM,Licht P

    更新日期:1985-11-01 00:00:00

  • Rodent insulin receptors are immunologically different from other mammalian insulin receptors.

    abstract::Four monoclonal antibodies (MA-5, MA-10, MA-20, and MA-51) and one polyclonal antibody (ARS-2) against human insulin receptor were used to immunoprecipitate the insulin receptor from several species which had been photolabeled with N epsilon B29-monoazido-benzoyl-[125I]iodoinsulin. All four monoclonal antibodies immun...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Tong ZQ,Jack E,Moule M,Goldfine ID,Yip CC

    更新日期:1994-06-01 00:00:00

  • Developmental expression patterns of FTZ-F1 homologues in zebrafish (Danio rerio).

    abstract::The fushi tarazu factor 1 (FTZ-F1) gene family constitutes a subgroup of orphan nuclear receptors which can be divided into two groups (LRH/FTF- and SF-1/Ad4BP-like) based on sequence homology, function, and tissue distribution. Analysis of zebrafish FTZ-F1 homologues (zFF1 and ff1b) during embryogenesis indicated dis...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: von Hofsten J,Jones I,Karlsson J,Olsson PE

    更新日期:2001-02-01 00:00:00

  • Distribution and subcellular localization of glucocorticoid receptor-immunoreactive neurons in the developing and adult male zebra finch brain.

    abstract::Stress has long lasting effects on physiology, development, behavior, reproductive success and the survival of an individual. These effects are mediated by glucocorticoids, such as corticosterone, via glucocorticoid receptors (GR), however the exact mechanisms underlying these effects are unknown. GR have been widely ...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Shahbazi M,Schmidt M,Carruth LL

    更新日期:2011-12-01 00:00:00

  • Changes in plasma hormone levels correlate with fledging in nestling Leach's storm-petrels.

    abstract::Leach's storm-petrels accumulate large amounts of body mass throughout the nestling period. Approximately 4days before fledging, nestlings weigh 50-100% more than adults. In order to shed this excess mass, nestlings engage in behavioral anorexia, and leave the burrow when they are light enough to fly. During this pref...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Kozlowski CP,Mauck RA,O'Reilly KM,Philipsborn J,Ricklefs RE

    更新日期:2010-10-01 00:00:00

  • Production of biologically active tethered tilapia LHbetaalpha by the methylotrophic yeast Pichia pastoris.

    abstract::In fish, luteinizing hormone (LH) stimulates processes leading to final oocyte maturation and ovulation in females, and spermiation in males. The hormone is a heterodimeric glycoprotein composed of two non-covalently associated subunits. In this study, we describe the expression of tilapia LH (tLH) as a biologically a...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Kasuto H,Levavi-Sivan B

    更新日期:2005-02-01 00:00:00

  • Spexin as a neuroendocrine signal with emerging functions.

    abstract::Spexin (SPX), a novel peptide coevolved with the galanin/kisspeptin family, was first identified by bioinformatics prior to its protein purification/functional studies. Its mature peptide is highly conserved among different vertebrate classes. Based on the studies in mammals and fish models, SPX was found to be widely...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章,评审


    authors: Ma A,Bai J,He M,Wong AOL

    更新日期:2018-09-01 00:00:00

  • Somatostatin-14 and somatostatin-25 stimulate glycogenolysis in rainbow trout, Oncorhynchus mykiss, liver incubated in vitro: a systemic role for somatostatins.

    abstract::The effects of somatostatin-14 (SS-14) and salmon somatostatin-25 (sSS-25) on hepatic glycogenolysis were studied by incubating rainbow trout liver pieces in vitro. Glycogen content in untreated liver pieces cultured for 3 and 5 hr was 28.6 +/- 7.6 mg/g fresh wt. and 21.5 +/- 6.6 U, respectively. Treatment of liver pi...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Eilertson CD,O'Connor PK,Sheridan MA

    更新日期:1991-05-01 00:00:00

  • Immunohistochemical localization of beta-endorphin-like material in the urodele and anuran amphibian tissues.

    abstract::In the present study, we have localized for the first time beta-endorphin (beta-EP)-like material in the adult and larval urodele and anuran tissues using immunohistochemical techniques. In the adult Notophthalmus viridescens and Ambystoma mexicanum, strong immunoreactivity to beta-EP antisera was observed in the regi...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Vethamany-Globus S

    更新日期:1989-08-01 00:00:00

  • Effects of recombinant eel growth hormone on the uptake of [35S]sulfate by ceratobranchial cartilages of the Japanese eel, Anguilla japonica.

    abstract::Effects of growth hormone (GH) on the synthesis of mucopolysaccharide by ceratobranchial cartilages of the Japanese eel, Anguilla japonica, were examined by monitoring the in vitro uptake of [35S]sulfate. The [35S]sulfate uptake decreased rapidly to one-third of the initial level during the first 3 days after hypophys...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Duan CM,Inui Y

    更新日期:1990-08-01 00:00:00

  • Age- and gender-associated changes in the concentrations of serum TGF-1β, DHEA-S and IGF-1 in healthy captive baboons (Papio hamadryas anubis).

    abstract::Age-related changes in the concentration of factors like TGF-1β, DHEA-S and IGF-1 may increase the risk of disease and illnesses in advanced life. A better understanding of these changes would aid in the development of more appropriate treatments and/or preventative care for many conditions associated with age. Due to...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Willis EL,Wolf RF,White GL,McFarlane D

    更新日期:2014-01-01 00:00:00

  • Anandamide and AM251, via water, modulate food intake at central and peripheral level in fish.

    abstract::The endocannabinoid system is a major regulator of food intake in many animal species. Studies conducted so far have mostly focused on mammals, and, therefore, in this study, the role of the endocannabinoid system in food intake in the sea bream Sparus aurata was investigated. The effect of different doses of the endo...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Piccinetti CC,Migliarini B,Petrosino S,Di Marzo V,Carnevali O

    更新日期:2010-04-01 00:00:00

  • Isolation and structural characterization of proglucagon-derived peptides, pancreatic polypeptide, and somatostatin from the urodele Amphiuma tridactylum.

    abstract::The expression of the preproglucagon gene in vertebrates is markedly species- and tissue-dependent. Three peptides derived from the posttranslational processing of preproglucagon were isolated from an extract of the pancreas of the urodele Amphiuma tridactylum (threetoed amphiuma). The primary structures of the peptid...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Cavanaugh ES,Nielsen PF,Conlon JM

    更新日期:1996-01-01 00:00:00

  • Effects of fasting on the central expression of appetite-regulating and reproductive hormones in wild-type and Casper zebrafish (Danio rerio).

    abstract::Appetite and reproduction are closely related functions that are both regulated by brain hormones. Appetite stimulators include orexin and neuropeptide Y (NPY), and reproductive hormones include gonadotropin-releasing hormone (GnRH), gonadotropin-inhibitory hormone (GnIH), kisspeptin, and neurokinin B (NKB). GnRH stim...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: London S,Volkoff H

    更新日期:2019-10-01 00:00:00

  • Effects of corticosteroids and protein synthesis inhibitors on activities of oxidative enzymes in a bony fish, Anabas testudineus (Bloch).

    abstract::The activities of oxidative enzymes of hepatic mitochondria of Anabas testudineus increased following exogenous administration of hydrocortisone and corticosterone. The inhibitors of protein synthesis prevented the hormone-mediated increase of hepatic enzymes, suggesting that corticosteroids regulate the oxidative met...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Ignatius J,Oommen OV

    更新日期:1990-05-01 00:00:00

  • Reproduction phase-related expression of GnRH-like immunoreactivity in the olfactory receptor neurons, their projections to the olfactory bulb and in the nervus terminalis in the female Indian major carp Cirrhinus mrigala (Ham.).

    abstract::The reproductive biology of the Indian major carp Cirrhinus mrigala is tightly synchronized with the seasonal changes in the environment. While the ovaries show growth from February through June, the fish spawn in July-August to coincide with the monsoon; thereafter the fish pass into the postspawning and resting phas...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Biju KC,Singru PS,Schreibman MP,Subhedar N

    更新日期:2003-10-01 00:00:00

  • Differential regulation of gonadotropins (FSH and LH) and growth hormone (GH) by neuroendocrine, endocrine, and paracrine factors in the zebrafish--an in vitro approach.

    abstract::Recently, zebrafish has quickly risen as a model species for functional analysis of the brain-pituitary-gonad axis. However, one of the hurdles for such work in this popular model organism is the small size of its pituitary gland, which makes it difficult to investigate the regulation of pituitary hormone expression a...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章


    authors: Lin SW,Ge W

    更新日期:2009-01-15 00:00:00

  • Consequences of steroid-5α-reductase deficiency and inhibition in vertebrates.

    abstract::In 1974, a lack of 5α-dihydrotestosterone (5α-DHT), the most potent androgen across species except for fish, was shown to be the origin of a type of pseudohermaphrodism in which boys have female-like external genitalia. This human intersex condition is linked to a mutation in the steroid-5α-reductase type 2 (SRD5α2) g...

    journal_title:General and comparative endocrinology

    pub_type: 杂志文章,评审


    authors: Robitaille J,Langlois VS

    更新日期:2020-05-01 00:00:00